DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. PLoS biology 2008 imex curation Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. DIP original experiment: DIP-68398X PLoS biology 2008 IMEx Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. PLoS biology 2008 imex curation Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. DIP original experiment: DIP-68393X PLoS biology 2008 IMEx Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. PLoS biology 2008 imex curation Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. DIP original experiment: DIP-68394X PLoS biology 2008 IMEx Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. PLoS biology 2008 imex curation Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. DIP original experiment: DIP-68397X PLoS biology 2008 IMEx Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. PLoS biology 2008 imex curation Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. DIP original experiment: DIP-68396X PLoS biology 2008 IMEx Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. PLoS biology 2008 imex curation Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. DIP original experiment: DIP-68392X PLoS biology 2008 IMEx Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. PLoS biology 2008 imex curation Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Xia F., Li J., Hickey GW., Tsurumi A., Larson K., Guo D., Yan SJ., Silver-Morse L., Li WX. DIP original experiment: DIP-68395X PLoS biology 2008 IMEx Raf activation is regulated by tyrosine 510 phosphorylation in Drosophila. kraf1_drome Raf homolog serine/threonine-protein kinase Raf Raf Protein pole-hole ph phl CG2845 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSESSTEGDSDLYDPLAEELHNVQLVKHVTRENIDALNAKFANLQEPPAMYLIEYQELTSKLHELEAKEQELMERLNSQDQQEDSSLVERFKEQPHYQNQTQILQQQRQLARVHHGNDLTDSLGSQPGSQCGTLTRQPKILLRAHLPNQQRTSVEVISGVRLCDALMKALKLRQLTPDMCEVSTTHSGRHIIPWHTDIGTLHVEEIFVRLLDKFPIRTHIKHQIIRKTFFSLVFCEGCRRLLFTGFYCSQCNFRFHQRCANRVPMLCQPFPMDSYYQLLLAENPDNGVGFPGRGTAVRFNMSSRSRSRRCSSSGSSSSSKPPSSSSGNHRQGRPPRISQDDRSNSAPNVCINNIRSVTSEVQRSLIMQARPPLPHPCTDHSNSTQASPTSTLKHNRPRARSADESNKNLLLRDAKSSEENWNILAEEILIGPRIGSGSFGTVYRAHWHGPVAVKTLNVKTPSPAQLQAFKNEVAMLKKTRHCNILLFMGCVSKPSLAIVTQWCEGSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDYLHAKNIIHRDLKSNNIFLHEDLSVKIGDFGLATAKTRWSGEKQANQPTGSILWMAPEVIRMQELNPYSFQSDVYAFGIVMYELLAECLPYGHISNKDQILFMVGRGLLRPDMSQVRSDAPQALKRLAEDCIKYTPKDRPLFRPLLNMLENMLRTLPKIHRSASEPNLTQSQLQNDEFLYLPSPKTPVNFNNFQFFGSAGNI DIP protein P11346 original sequence version: 98 DIP protein P11346 original sequence version: 96 DIP protein P11346 original sequence version: 130 B685A895D05048C1 dsor1_drome Dual specificity mitogen-activated protein kinase kinase dSOR1 Dsor1 CG15793 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSKNKLNLVLPPVNTEATVAAATVAPTPPFKTPSGTDTHSLLGKPKTSIDALTETLEGLDMGDTERKRIKMFLSQKEKIGELSDEDLEKLGELGSGNGGVVMKVRHTHTHLIMARKLIHLEVKPAIKKQILRELKVLHECNFPHIVGFYGAFYSDGEISICMEYMDGGSLDLILKRAGRIPESILGRITLAVLKGLSYLRDNHAIIHRDVKPSNILVNSSGEIKICDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSLGLSLVEMAIGMYPIPPPNTATLESIFADNAEESGQPTDEPRAMAIFELLDYIVNEPPPKLEHKIFSTEFKDFVDICLKKQPDERADLKTLLSHPWIRKAELEEVDISGWVCKTMDLPPSTPKRNTSPN DIP protein Q24324 original sequence version: 68 DIP protein Q24324 original sequence version: 97 9245CED1DA750C33 src64_drome Tyrosine-protein kinase Src64B Src64B Src1 CG7524 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGNKCCSKRQDQELALAYPTGGYKKSDYTFGQTHINSSGGGNMGGVLGQKHNNGGSLDSRYTPDPNHRGPLKIGGKGGVDIIRPRTTPTGVPGVVLKRVVVALYDYKSRDESDLSFMKGDRMEVIDDTESDWWRVVNLTTRQEGLIPLNFVAEERSVNSEDWFFENVLRKEADKLLLAEENPRGTFLVRPSEHNPNGYSLSVKDWEDGRGYHVKHYRIKPLDNGGYYIATNQTFPSLQALVMAYSKNALGLCHILSRPCPKPQPQMWDLGPELRDKYEIPRSEIQLLRKLGRGNFGEVFYGKWRNSIDVAVKTLREGTMSTAAFLQEAAIMKKFRHNRLVALYAVCSQEEPIYIVQEYMSKGSLLDFLREGDGRYLHFEDLIYIATQVASGMEYLESKQLIHRDLAARNVLIGENNVAKICDFGLARVIADDEYCPKQGSRFPVKWTAPEAIIYGKFSIKSDVWSYGILLMELFTYGQVPYPGMHSREVIENIERGFRMPKPTNHYFPDNIYQLLLQCWDAVPEKRPTFEFLNHYFESFSVTSEVPYREVQD DIP protein P00528 original sequence version: 104 4A63CF4F16562864 phl-phl-1 5 8 unspecified role unspecified role bait bait Region 1-367 Region 1-367 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role prey prey Region 368-739 Region 368-739 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P11346:p.Tyr510Glu Y510E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y E This feature has been corrected as a result of our quality control procedures. The original label was 'Y510E'. Wed May 02 18:08:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr510glu' Tue Jun 30 15:46:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr510Glu' V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association phl-src64b-1 3 10 enzyme enzyme unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 368-739 Region 368-739 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P11346:p.Tyr510Phe Y510F mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y F This feature has been corrected as a result of our quality control procedures. The original label was 'Y510F'. Wed May 02 14:45:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr510phe' Tue Jun 30 16:56:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr510Phe' His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction phl-src64b-2 6 10 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role bait bait Region 1-367 Region 1-367 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association dsor1-phl-1 1 9 enzyme target enzyme target substrate substrate unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 enzyme enzyme unspecified role unspecified role P11346:p.Tyr510Glu Y510E mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y E This feature has been corrected as a result of our quality control procedures. The original label was 'Y510E'. Wed May 02 15:08:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr510glu' Tue Jun 30 17:23:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr510Glu' V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction src64b-phl-1 2 10 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association dsor1-phl-2 7 9 enzyme target enzyme target substrate substrate unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 enzyme enzyme unspecified role unspecified role Region 368-739 Region 368-739 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction dsor1-phl-3 4 9 enzyme target enzyme target substrate substrate unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 enzyme enzyme unspecified role unspecified role P11346:p.Tyr510Glu Y510E mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y E This feature has been corrected as a result of our quality control procedures. The original label was 'Y510E'. Wed May 02 16:22:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr510glu' Tue Jun 30 16:33:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr510Glu' ecolx Escherichia coli direct interaction direct interaction