DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. Structure (London, England : 1993) 2008 imex curation Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay anti tag western anti tag western blot anti tag western Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. DIP original experiment: DIP-68408X Structure (London, England : 1993) 2008 IMEx The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. Structure (London, England : 1993) 2008 imex curation Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. DIP original experiment: DIP-68403X Structure (London, England : 1993) 2008 IMEx The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. Structure (London, England : 1993) 2008 imex curation Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. DIP original experiment: DIP-68404X Structure (London, England : 1993) 2008 IMEx The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. Structure (London, England : 1993) 2008 imex curation Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. DIP original experiment: DIP-68405X Structure (London, England : 1993) 2008 IMEx The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. Structure (London, England : 1993) 2008 imex curation Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. DIP original experiment: DIP-68407X Structure (London, England : 1993) 2008 IMEx The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. Structure (London, England : 1993) 2008 imex curation Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay anti tag western anti tag western blot anti tag western Koenig P., Oreb M., Höfle A., Kaltofen S., Rippe K., Sinning I., Schleiff E., Tews I. DIP original experiment: DIP-68406X Structure (London, England : 1993) 2008 IMEx The GTPase cycle of the chloroplast import receptors Toc33/Toc34: implications from monomeric and dimeric structures. toc34_arath Translocase of chloroplast 34, chloroplastic TOC34 OEP34 MUG13.14 At5g05000 PPI3 Plastid protein import 3 34 kDa chloroplast outer envelope protein GTP-binding protein OEP34 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEKAAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVLLYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSNALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAWELRDSGLASRRS DIP protein Q38906 original sequence version: 66 1C90E435B2EE0441 toc34_pea Translocase of chloroplast 34 34 kDa chloroplast outer envelope protein GTP-binding protein OEP34 GTP-binding protein IAP34 TOC34 IAP34 OEP34 protein protein pea Pisum sativum (Garden pea) Garden pea MASQQQTVREWSGINTFAPATQTKLLELLGNLKQEDVNSLTILVMGKGGVGKSSTVNSIIGERVVSISPFQSEGPRPVMVSRSRAGFTLNIIDTPGLIEGGYINDMALNIIKSFLLDKTIDVLLYVDRLDAYRVDNLDKLVAKAITDSFGKGIWNKAIVALTHAQFSPPDGLPYDEFFSKRSEALLQVVRSGASLKKDAQASDIPVVLIENSGRCNKNDSDEKVLPNGIAWIPHLVQTITEVALNKSESIFVDKNLIDGPNPNQRGKLWIPLIFALQYLFLAKPIEALIRRDIATETKPAWETRDVGDRK DIP protein Q41009 original sequence version: 60 DIP protein Q41009 original sequence version: 90 00D5E79735BF041F toc34-1 2 8 unspecified role unspecified role unspecified role unspecified role Region 1-266 Region 1-266 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction toc34-toc34-1 6 8 unspecified role unspecified role prey prey Region 1-266 Region 1-266 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 8 unspecified role unspecified role bait bait Region 1-266 Region 1-266 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli direct interaction direct interaction toc34-2 3 8 unspecified role unspecified role unspecified role unspecified role Region 1-266 Region 1-266 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction toc34-6 4 8 unspecified role unspecified role unspecified role unspecified role Region 1-266 Region 1-266 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction toc34-4 5 7 unspecified role unspecified role unspecified role unspecified role Region 1-251 Region 1-251 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction toc34-toc34-2 1 7 unspecified role unspecified role prey prey Region 1-251 Region 1-251 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 7 unspecified role unspecified role bait bait Region 1-251 Region 1-251 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli direct interaction direct interaction