DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. DIP original experiment: DIP-68317X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. DIP original experiment: DIP-68322X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. DIP original experiment: DIP-68320X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. DIP original experiment: DIP-68318X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. DIP original experiment: DIP-68319X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. DIP original experiment: DIP-68321X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Rajakulendran T., Sahmi M., Kurinov I., Tyers M., Therrien M., Sicheri F. DIP original experiment: DIP-68316X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling. q24171_drome ksr CG2899 Dmel\CG2899 EC3-1 EK3-1 KSR KSR-1 Ksr Ksr-1 SR3-1 dKsr l(3)j5E2 Dmel_CG2899 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSNNNAPASAPDTGSTNANDPISGSLSVDSNLVIIQDMIDLSANHLEGLRTQCAISSTLTQQEIRCLESKLVRYFSELLLAKMRLNERIPANGLVPHTTGNELRQWLRVVGLSQGTLTACLARLTTLEQSLRLSDEEIRQLLADSPSQREEEELRRLTRAMQNLRKCMESLESGTAASNNDPEQWHWDSWDRPTHIHRGSVGNIGLGNNSTASPRTHHRQHGVKGKNSALANSTNFKSGRQSPSATEELNSTQGSQLTLTLTPSPPNSPFTPSSGLSSSLNGTPQRSRGTPPPARKHQTLLSQSHVQVDGEQLARNRLPTDPSPDSHSSTSSDIFVDPNTNASSGGSSSNVLMVPCSPGVGHVGMGHAIKHRFTKALGFMATCTLCQKQVFHRWMKCTDCKYICHKSCAPHVPPSCGLPREYVDEFRHIKEQGGYASLPHVHGAAKGSPLVKKSTLGKPLHQQHGDSSSPSSSCTSSTPSSPALFQQRERELDQAGSSSSANLLPTPSLGKHQPSQFNFPNVTVTSSGGSGGVSLISNEPVPEQFPTAPATANGGLDSLVSSSNGHMSSLIGSQTSNASTAATLTGSLVNSTTTTSTCSFFPRKLSTAGVDKRTPFTSEYTDTHKSNDSDKTVSLSGSASTDSDRTPVRVDSTEDGDSGQWRQNSISLKEWDIPYGDLLLLERIGQGRFGTVHRALWHGDVAVKLLNEDYLQDEHMLETFRSEVANFKNTRHENLVLFMGACMNPPYLAIVTSLCKGNTLYTYIHQRREKFAMNRTLLIAQQIAQGMGYLHAREIIHKDLRTKNIFIENGKVIITDFGLFSSTKLLYCDMGLGVPHNWLCYLAPELIRALQPEKPRGECLEFTPYSDVYSFGTVWYELICGEFTFKDQPAESIIWQVGRGMKQSLANLQSGRDVKDLLMLCWTYEKEHRPQFARLLSLLEHLPKKRLARSPSHPVNLSRSAESVF DIP protein Q24171 original sequence version: 71 DIP protein Q24171 original sequence version: 109 EA9989C39AA161BF ave_drome Protein aveugle ave CG30476 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGEETINSTQNKTRTKTTRPKAVYLWTVSDVLKWYRRHCGEYTQYEQLFAQHDITGRALLRITDSSLQRMGVTDNRDREAIWREIVKQRLKTDIMEIRDMERLNIY DIP protein Q8ML92 original sequence version: 39 7DB605A43989F997 cnkr2_human Connector enhancer of kinase suppressor of ras 2 CNKSR2 CNK2 KIAA0902 KSR2 CNK homolog protein 2 protein protein human Homo sapiens Human MALIMEPVSKWSPSQVVDWMKGLDDCLQQYIKNFEREKISGDQLLRITHQELEDLGVSRIGHQELILEAVDLLCALNYGLETENLKTLSHKLNASAKNLQNFITGRRRSGHYDGRTSRKLPNDFLTSVVDLIGAAKSLLAWLDRSPFAAVTDYSVTRNNVIQLCLELTTIVQQDCTVYETENKILHVCKTLSGVCDHIISLSSDPLVSQSAHLEVIQLANIKPSEGLGMYIKSTYDGLHVITGTTENSPADRCKKIHAGDEVIQVNHQTVVGWQLKNLVNALREDPSGVILTLKKRPQSMLTSAPALLKNMRWKPLALQPLIPRSPTSSVATPSSTISTPTKRDSSALQDLYIPPPPAEPYIPRDEKGNLPCEDLRGHMVGKPVHKGSESPNSFLDQEYRKRFNIVEEDTVLYCYEYEKGRSSSQGRRESTPTYGKLRPISMPVEYNWVGDYEDPNKMKRDSRRENSLLRYMSNEKIAQEEYMFQRNSKKDTGKKSKKKGDKSNSPTHYSLLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGPIAGKSKRRISCKDLGRGDCEGWLWKKKDAKSYFSQKWKKYWFVLKDASLYWYINEEDEKAEGFISLPEFKIDRASECRKKYAFKACHPKIKSFYFAAEHLDDMNRWLNRINMLTAGYAERERIKQEQDYWSESDKEEADTPSTPKQDSPPPPYDTYPRPPSMSCASPYVEAKHSRLSSTETSQSQSSHEEFRQEVTGSSAVSPIRKTASQRRSWQDLIETPLTSSGLHYLQTLPLEDSVFSDSAAISPEHRRQSTLPTQKCHLQDHYGPYPLAESERMQVLNGNGGKPRSFTLPRDSGFNHCCLNAPVSACDPQDDVQPPEVEEEEEEEEEEGEAAGENIGEKSESREEKLGDSLQDLYRALEQASLSPLGEHRISTKMEYKLSFIKRCNDPVMNEKLHRLRILKSTLKAREGEVAIIDKVLDNPDLTSKEFQQWKQMYLDLFLDICQNTTSNDPLSISSEVDVITSSLAHTHSYIETHV DIP protein Q8WXI2 original sequence version: 52 E43DB0D8D72D954C q7knq9_drome cnk CNK Cnk Dmel\CG6556 EC2-3 EK2-3 anon-WO0140519.129 anon-WO0257455.27 l(2)k16314 sag CG6556 Dmel_CG6556 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAYINIAEWTPDQVTDWIKGLDESMKGYLYEFSKQEIGGRALLNIRPYELENLGMLRIGHQEIVLEAVENLRNFHYHLKNDNLQFMALHVATAAKNLHRELARNHAESTKIDTRILHDITRTIATLKPLVGSLERTPFRKQEMYREYCGNVLKCGLELATIAHRDRFALQPVPAIRQSAERLENLANFVIQDISDPMVLQPASLNLVTLKKRESELGFNIESSYNGIHRVTDIKYNSPAHNSGKIEDGDEIVQINYQTVVGWQHRTVLEHLREALPDVVLTVKKRPKHTKMFGQIYMQPYRLPSKKRNMAARWAAQMPSPRAAFLTLDTEQLATGGTGSAVPDPSKSLSSEKREVLTKANPVSSASDSDSSCSDIPTPTDPKLAAREIRLYYPKPRALLQRRNTICGCEYLSLKNSDLVVPSWHERKPGIGSPTNCDPGSPSIRDKSISFGYGLEMAARPTTCIGIAGDTSTDKARRMFHEARKLKQLTEDSQREFLVDRYKPGVSKVVRFDAKEDYVMKNEKFICNVENTILETFEPIPFADEGDEDALETLRNCKTENAEELLEAINLATKGQDLPLAEAINMPLLQQGRRGRLDKSHSTPAYDNSGEESDTPPAIEPRKEFLLVTPPAPPPRPRKQREMTPPAVPPPPPKPASMQPASSITSISIPVPVPVPAAVDPSEISELHTPSKSRTLTLKKKHSLMAKRRNTNLKLLGTGDIQGHLYRRKKNHRGVTYWARIYFVMLDTILYGFRSKQSTSASLVIFLPGFTVSLAKEVHSKPHAFKVYHTAKSFYFAAESLDALNQWVDFLRQASLKVPPSTGSKGGGDAKDLYSENDSSGEECDALVIQNLSTPSPQGNKESMSMSMTLSGGTPPSSAPIKHERGYLDSFRKFTNTFKSSAAKPSSDIPVPTEQYRSYRKVPGGSFGIQIGANTPGYHDPAMPPTQIPPLVGPKLSRSSSRSSVVSGTESAVSLSASILGPPASPAPTPTPTATPTSLQHQSSEESPSQSQSQSPSSKSSLKKAPFNFLHASNPNLVEFDFHTSKTLLPKMSVGNTLDHGHNIQGFVTLKDLMLRKQEEEAQEMYNNRVHLGVEKHKHARTESTASQQSAMSSSVTKPLEKLPKIQSVSLPKTPDYEISFKPDDESIKRTRTKEGQKLRDFGYELICGDEPSTSSISRQEHHHHHHHHQHHLQHVQQQQQLQHQQTQQQHSSKAKHFLRSQQLQLSSFLHKNSSGGSGKSSGSGADQKKSKGKSSGDRRFPFSKHSTGSAGSGCGPGHAMTMTLPLNKKSKSNHALDGAVGNGVTIIGSGSSIKKSQTYNQDLRDKIVGTKYDAHRKNSAPIPIFSKLSISGGTPAKPSKENRFLGSPLLHRTLFGHHHHQQQAVTPPSSADPDCDQEIFSQITLPTHNQAGYRSYRGPGILTTSTTNLCSSEGLQPPLPPAPPPPVNTSRQREEEQAEATISECAATPKVSSSGSVDSKAATPDYPNMECPPVFEPEIYSLSDTSLSRIVMRTPSSSGATSATASTTTTNNNNNNNNTNNNTNTNSSPSGGEHHQT DIP protein Q7KNQ9 original sequence version: 20 4A1D0E9B0AE24281 ave-cnksr2-1 3 9 unspecified role unspecified role bait bait Region 21-98 Region 21-98 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8ML92:p.[Arg57Glu;Arg61Glu] R57E/R61E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R57E/R61E'. Wed May 02 17:51:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg57glu,arg61glu' Tue Jun 30 16:02:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg57Glu;Arg61Glu]' Q8ML92:p.[Arg57Ala;Arg61Ala] R57A/R61A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R57A/R61A'. Wed May 02 20:02:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg57ala,arg61ala' Tue Jun 30 18:56:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg57Ala;Arg61Ala]' Q8ML92:p.Arg61Glu R61E mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R61E'. Wed May 02 17:52:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg61glu' Tue Jun 30 16:47:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg61Glu' ecolx Escherichia coli 10 unspecified role unspecified role prey prey Region 5-84 Region 5-84 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q8WXI2:p.[Glu68Arg;Asp71Arg] E68R/D71R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D R certain certain sequence position certain certain certain sequence position certain E R This feature has been corrected as a result of our quality control procedures. The original label was 'E68R/D71R'. Wed May 02 17:53:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu68arg,asp71arg' Tue Jun 30 16:47:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu68Arg;Asp71Arg]' Q8WXI2:p.Asp71Arg D71R mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D R This feature has been corrected as a result of our quality control procedures. The original label was 'D71R'. Wed May 02 16:54:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp71arg' Tue Jun 30 19:47:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp71Arg' Q8WXI2:p.Ile60Ala I60A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I60A'. Wed May 02 16:35:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile60ala' Tue Jun 30 16:04:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile60Ala' His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction ave-cnksr2-2 4 9 unspecified role unspecified role bait bait Region 21-98 Region 21-98 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role prey prey Region 5-84 Region 5-84 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ave-cnk-4 6 11 unspecified role unspecified role prey prey Region 2-549 Region 2-549 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q7KNQ9:p.[Glu66Arg;Glu69Arg] E66R/E69R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E R certain certain sequence position certain certain certain sequence position certain E R This feature has been corrected as a result of our quality control procedures. The original label was 'E66R/E69R'. Wed May 02 19:41:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu66arg,glu69arg' Tue Jun 30 18:20:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu66Arg;Glu69Arg]' drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role bait bait Q8ML92:p.[Arg57Ala;Arg61Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:23:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg57ala,arg61ala' Tue Jun 30 17:54:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg57Ala;Arg61Ala]' GST-tag GST-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8ML92:p.[Arg57Asp;Arg61Asp] R57A/D/R61A/D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R D certain certain sequence position certain certain certain sequence position certain R D Wed May 02 18:39:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg57asp,arg61asp' Tue Jun 30 20:20:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg57Asp;Arg61Asp]' Q8ML92:p.Arg61Asp R61D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R D This feature has been corrected as a result of our quality control procedures. The original label was 'R61D'. Wed May 02 20:21:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg61asp' Tue Jun 30 18:46:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg61Asp' ecolx Escherichia coli physical association physical association ave-cnksr2-3 7 9 unspecified role unspecified role unspecified role unspecified role Region 21-98 Region 21-98 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 5-84 Region 5-84 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ave-cnksr2-4 5 9 unspecified role unspecified role bait bait Region 21-98 Region 21-98 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role prey prey Region 5-84 Region 5-84 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ave-cnksr2-5 1 9 unspecified role unspecified role unspecified role unspecified role Region 21-98 Region 21-98 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role Region 5-84 Region 5-84 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ave-cnk-5 2 8 unspecified role unspecified role prey prey Region 656-966 Region 656-966 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 11 unspecified role unspecified role prey prey Region 2-549 Region 2-549 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly 9 unspecified role unspecified role bait bait GST-tag GST-tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli association association