DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. Only protein-protein interactions mouse Mus musculus Mouse molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. DIP original experiment: DIP-68480X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. DIP original experiment: DIP-68477X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx f.4 Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. DIP original experiment: DIP-68475X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. DIP original experiment: DIP-68481X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. DIP original experiment: DIP-68479X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. DIP original experiment: DIP-68478X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. DIP original experiment: DIP-68476X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx f.4 Signal processing by its coil zipper domain activates IKK gamma. Signal processing by its coil zipper domain activates IKK gamma. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Bloor S., Ryzhakov G., Wagner S., Butler PJ., Smith DL., Krumbach R., Dikic I., Randow F. DIP original experiment: DIP-68482X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Signal processing by its coil zipper domain activates IKK gamma. ikka_mouse Inhibitor of nuclear factor kappa-B kinase subunit alpha I-kappa-B kinase 1 Conserved helix-loop-helix ubiquitous kinase Nuclear factor NF-kappa-B inhibitor kinase alpha Chuk Ikka protein protein mouse Mus musculus Mouse MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIKSCRLELSSKNRERWCHEIQIMKKLDHANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKTIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNSLCSLIVEPMESWLQLMLNWDPQQRGGPIDLTLKQPRCFALMDHILNLKIVHILNMTSAKIISFLLPCDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPPDHLYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGTVTPPVSAWLPPTLADREHPLTCVVTPQDGETLAQMIEENLNCLGHLSTIIREANEDQSSSLMSLDWSWLAE DIP protein Q60680 original sequence version: 82 DIP protein Q60680 original sequence version: 86 DIP protein Q60680 original sequence version: 113 DIP protein Q60680 original sequence version: 126 DIP protein Q60680 original sequence version: 158 3FEF5582AFF92233 traf6_mouse TNF receptor-associated factor 6 RING-type E3 ubiquitin transferase TRAF6 Traf6 E3 ubiquitin-protein ligase TRAF6 protein protein mouse Mus musculus Mouse MSLLNCENSCGSSQSSSDCCAAMAASCSAAVKDDSVSGSASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVNCPQCQRPFQKCQVNTHIIEDCPRRQVSCVNCAVSMAYEEKEIHDQSCPLANIICEYCGTILIREQMPNHYDLDCPTAPIPCTFSVFGCHEKMQRNHLARHLQENTQLHMRLLAQAVHNVNLALRPCDAASPSRGCRPEDPNYEETIKQLESRLVRQDHQIRELTAKMETQSMYVGELKRTIRTLEDKVAEMEAQQCNGIYIWKIGNFGMHLKSQEEERPVVIHSPGFYTGRPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIKDDTLLVRCEVSTRFDMGGLRKEGFQPRSTDAGV DIP protein P70196 original sequence version: 139 DIP protein P70196 original sequence version: 128 DIP protein P70196 original sequence version: 144 DIP protein P70196 original sequence version: 83 DIP protein P70196 original sequence version: 98 DIP protein P70196 original sequence version: 104 DIP protein P70196 original sequence version: 112 DIP protein P70196 original sequence version: 163 F8389250425E4E2D ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ikkb_mouse Inhibitor of nuclear factor kappa-B kinase subunit beta I-kappa-B kinase 2 Nuclear factor NF-kappa-B inhibitor kinase beta Ikbkb Ikkb Serine/threonine protein kinase IKBKB protein protein mouse Mus musculus Mouse MSWSPSLPTQTCGAWEMKERLGTGGFGNVIRWHNQATGEQIAIKQCRQELSPKNRNRWCLEIQIMRRLNHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRRYLNQFENCCGLREGAVLTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEKRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGAVKFSSSLPFPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPQYGPNGCFRALDDILNLKLVHVLNMVTGTVHTYPVTEDESLQSLKTRIQENTGILETDQELLQKAGLVLLPDKPATQCISDSKTNEGLTLDMDLVFLLDNSKINYETQITPRPPPESVSCILQEPKRNLSFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMSLLRNNSCLSKMKNAMASTAQQLKAKLDFFKTSIQIDLEKYKEQTEFGITSDKLLLAWREMEQAVEQCGRENDVKHLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRKLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDERTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNVSRLSHPGQLMSQPSSACDSLPESDKKSEELVAEAHALCSRLESALQDTVKEQDRSFTTLDWSWLQMEDEERCSLEQACD DIP protein O88351 original sequence version: 126 DIP protein O88351 original sequence version: 73 DIP protein O88351 original sequence version: 99 DIP protein O88351 original sequence version: 114 DIP protein O88351 original sequence version: 149 FED962F095449C5E nemo_mouse NF-kappa-B essential modulator NF-kappa-B essential modifier Inhibitor of nuclear factor kappa-B kinase subunit gamma IkB kinase-associated protein 1 Ikbkg Nemo protein protein mouse Mus musculus Mouse MNKHPWKNQLSEMVQPSGGPAEDQDMLGEESSLGKPAMLHLPSEQGTPETLQRCLEENQELRDAIRQSNQMLRERCEELLHFQVSQREEKEFLMCKFQEARKLVERLSLEKLDLRSQREQALKELEQLKKCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKDRQALEGRIRAVSEQVRQLESEREVLQQQHSVQVDQLRMQNQSVEAALRMERQAASEEKRKLAQLQAAYHQLFQDYDSHIKSSKGMQLEDLRQQLQQAEEALVAKQELIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERHAREKLVEKKEYLQEQLEQLQREFNKLKVGCHESARIEDMRKRHVETPQPPLLPAPAHHSFHLALSNQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE DIP protein O88522 original sequence version: 115 DIP protein O88522 original sequence version: 63 DIP protein O88522 original sequence version: 91 DIP protein O88522 original sequence version: 90 DIP protein O88522 original sequence version: 104 DIP protein O88522 original sequence version: 138 66C693C857A2D5E6 ikbkg-traf6-1 2 10 unspecified role unspecified role prey prey myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 13 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association ub-ikbkg-1 3 11 unspecified role unspecified role bait bait GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 13 unspecified role unspecified role prey prey 250-338 binding site 250-338 binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction ikbkb-ikbkg-1 1 12 unspecified role unspecified role neutral component neutral component mouse Mus musculus Mouse 13 unspecified role unspecified role neutral component neutral component mouse Mus musculus Mouse physical association physical association ub-ikbkg-2 8 11 unspecified role unspecified role bait bait ecolx Escherichia coli 13 unspecified role unspecified role prey prey AU1 tag AU1 tag tag tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association ikbkg-ikbkg-2 6 13 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 13 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ikbkg-ub-3 7 11 unspecified role unspecified role prey prey myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal human Homo sapiens Human 13 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association ub-ikbkg-3 4 11 unspecified role unspecified role bait bait GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ile44ala I44A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I44A mutation'. ecolx Escherichia coli 13 unspecified role unspecified role prey prey luciferase tag luciferase tag renilla luciferase renilla luciferase protein tag renilla luciferase predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association ikbkg-chuk-9 5 12 unspecified role unspecified role prey prey mouse Mus musculus Mouse 9 unspecified role unspecified role prey prey mouse Mus musculus Mouse 13 unspecified role unspecified role bait bait mouse Mus musculus Mouse association association