IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition. The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition. Immunity 2007 imex curation Sullivan LC., Clements CS., Beddoe T., Johnson D., Hoare HL., Lin J., Huyton T., Hopkins EJ., Reid HH., Wilce MC., Kabat J., Borrego F., Coligan JE., Rossjohn J., Brooks AG. DSP=86? jamie.rossjohn@med.monash.edu.au,agbrooks@unimelb.edu.au 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sullivan LC., Clements CS., Beddoe T., Johnson D., Hoare HL., Lin J., Huyton T., Hopkins EJ., Reid HH., Wilce MC., Kabat J., Borrego F., Coligan JE., Rossjohn J., Brooks AG. DSP=86? jamie.rossjohn@med.monash.edu.au,agbrooks@unimelb.edu.au Accepted 2017-MAY-31 AT 11:33 BST AT 11:33 BST by ORCHARD Immunity 2007 imex curation added Kds as suggested The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition. The heterodimeric assembly of the CD94-NKG2 receptor family and implications for human leukocyte antigen-E recognition. Immunity 2007 imex curation Sullivan LC., Clements CS., Beddoe T., Johnson D., Hoare HL., Lin J., Huyton T., Hopkins EJ., Reid HH., Wilce MC., Kabat J., Borrego F., Coligan JE., Rossjohn J., Brooks AG. DSP=86? jamie.rossjohn@med.monash.edu.au,agbrooks@unimelb.edu.au 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Sullivan LC., Clements CS., Beddoe T., Johnson D., Hoare HL., Lin J., Huyton T., Hopkins EJ., Reid HH., Wilce MC., Kabat J., Borrego F., Coligan JE., Rossjohn J., Brooks AG. I added Kd values for mutants in table S2 (only a few) - but then saw Table 2 and decided not to add any more. Send back if you really want to Kd Values added for every mutant. I have (approximately) meant to add "mutation with no effect/decreasing/disrupting -as per text, notably: "less than a 3-fold change in affinity was insignificant, a 3- to 8-fold indicated a moderate effect, and >8-fold indicated a significant effect on the interaction." DSP=86? jamie.rossjohn@med.monash.edu.au,agbrooks@unimelb.edu.au Accepted 2017-MAY-31 AT 11:33 BST AT 11:33 BST by ORCHARD Immunity 2007 imex curation added Kds as suggested nkg2c_human NKG2-C type II integral membrane protein KLRC2 NKG2C CD159 antigen-like family member C NK cell receptor C NKG2-C-activating NK receptor protein protein human Homo sapiens Human MSKQRGTFSEVSLAQDPKRQQRKPKGNKSSISGTEQEIFQVELNLQNPSLNHQGIDKIYDCQGLLPPPEKLTAEVLGIICIVLMATVLKTIVLIPFLEQNNSSPNTRTQKARHCGHCPEEWITYSNSCYYIGKERRTWEESLLACTSKNSSLLSIDNEEEMKFLASILPSSWIGVFRNSSHHPWVTINGLAFKHKIKDSDNAELNCAVLQVNRLKSAQCGSSMIYHCKHKL nkg2a_human NKG2-A/NKG2-B type II integral membrane protein KLRC1 CD159 antigen-like family member A NK cell receptor A NKG2-A/B-activating NK receptor NKG2A protein protein human Homo sapiens Human MDNQGVIYSDLNLPPNPKRQQRKPKGNKNSILATEQEITYAELNLQKASQDFQGNDKTYHCKDLPSAPEKLIVGILGIICLILMASVVTIVVIPSTLIQRHNNSSLNTRTQKARHCGHCPEEWITYSNSCYYIGKERRTWEESLLACTSKNSSLLSIDNEEEMKFLSIISPSSWIGVFRNSSHHPWVTMNGLAFKHEIKDSDNAELNCAVLQVNRLKSAQCGSSIIYHCKHKL 93879A5C8D110C62 klrd1_human Natural killer cells antigen CD94 KLRD1 CD94 KP43 Killer cell lectin-like receptor subfamily D member 1 NK cell receptor protein protein human Homo sapiens Human MAVFKTTLWRLISGTLGIICLSLMSTLGILLKNSFTKLSIEPAFTPGPNIELQKDSDCCSCQEKWVGYRCNCYFISSEQKTWNESRHLCASQKSSLLQLQNTDELDFMSSSQQFYWIGLSYSEEHTAWLWENGSALSQYLFPSFETFNTKNCIAYNPNGNALDESCEDKNRYICKQQLI 01634D3832D4B1A7 hlae_human HLA class I histocompatibility antigen, alpha chain E MHC class I antigen E HLA-E HLA-6.2 HLAE protein protein human Homo sapiens Human MVDGTLLLLLSEALALTQTWAGSHSLKYFHTSVSRPGRGEPRFISVGYVDDTQFVRFDNDAASPRMVPRAPWMEQEGSEYWDRETRSARDTAQIFRVNLRTLRGYYNQSEAGSHTLQWMHGCELGPDGRFLRGYEQFAYDGKDYLTLNEDLRSWTAVDTAAQISEQKSNDASEAEHQRAYLEDTCVEWLHKYLEKGKETLLHLEPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQQDGEGHTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPVTLRWKPASQPTIPIVGIIAGLVLLGSVVSGAVVAAVIWRKKSSGGKGGSYSKAEWSDSAQGSESHSL 6B3001CA9F3B7FE3 hlag_human HLA class I histocompatibility antigen, alpha chain G HLAG HLA-G HLA-6.0 HLA G antigen MHC class I antigen G protein protein human Homo sapiens Human MVVMAPRTLFLLLSGALTLTETWAGSHSMRYFSAAVSRPGRGEPRFIAMGYVDDTQFVRFDSDSACPRMEPRAPWVEQEGPEYWEEETRNTKAHAQTDRMNLQTLRGYYNQSEASSHTLQWMIGCDLGSDGRLLRGYEQYAYDGKDYLALNEDLRSWTAADTAAQISKRKCEAANVAEQRRAYLEGTCVEWLHRYLENGKEMLQRADPPKTHVTHHPVFDYEATLRCWALGFYPAEIILTWQRDGEDQTQDVELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLMLRWKQSSLPTIPIMGIVAGLVVLAAVVTGAAVAAVLWRKKSSD DIP protein P17693 original sequence version: 150 DIP protein P17693 original sequence version: 89 0678E263BE8962FF p61769-pro_0000018779 Beta-2-microglobulin B2M CDABP0092 HDCMA22P protein protein human Homo sapiens Human IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM 21 119 B6EE395752B3322D klrc1-klrd1-1 1 4 unspecified role unspecified role neutral component neutral component ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 11 direct interaction direct interaction Table 1 2.5A RFree =0.27 RWork =0.234 We describe the 2.5 resolution crystal structure of CD94-NKG2A 245trwesfs-1 2 7 unspecified role unspecified role ancillary ancillary leader_sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P17693:p.Phe10Ile mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F I Wed May 02 18:41:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe10ile' Tue Jun 30 18:30:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe10Ile' P17693:p.Phe10Leu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F L Wed May 02 15:56:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe10leu' Tue Jun 30 17:43:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe10Leu' P17693:p.Phe10Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F V Wed May 02 15:57:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe10val' Tue Jun 30 19:29:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe10Val' P17693:p.Phe10Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 19:50:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe10ala' Tue Jun 30 17:11:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe10Ala' P17693:p.Phe10Tyr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F Y Wed May 02 17:55:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe10tyr' Tue Jun 30 19:52:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe10Tyr' P17693:p.Phe10Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F K Wed May 02 16:02:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe10lys' Tue Jun 30 19:05:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe10Lys' chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 24 16 26 28 30 physical association physical association Fig S2, Table S2 HLA-E∗0103 allele klrc1-b2m-1 2 7 unspecified role unspecified role ancillary ancillary leader_sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey P13747:p.Arg86Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:55:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg86ala' Tue Jun 30 20:23:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg86Ala' P13747:p.Asp90Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Wed May 02 18:15:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp90ala' Tue Jun 30 19:58:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp90Ala' P13747:p.Gln93Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 14:38:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln93ala' Tue Jun 30 17:10:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln93Ala' P13747:p.Arg96Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 19:01:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg96ala' Tue Jun 30 16:58:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg96Ala' P13747:p.Val97Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A Wed May 02 17:39:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val97ala' Tue Jun 30 17:15:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val97Ala' P13747:p.Arg100Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:30:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg100ala' Tue Jun 30 18:38:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg100Ala' P13747:p.Glu110Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:45:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu110ala' Tue Jun 30 17:58:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu110Ala' P13747:p.Arg129Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 17:46:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg129ala' Tue Jun 30 16:26:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg129Ala' P13747:p.Asp170Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:54:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp170ala' Tue Jun 30 15:54:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp170Ala' P13747:p.Glu175Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:55:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu175ala' Tue Jun 30 15:54:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu175Ala' P13747:p.His176Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H A Wed May 02 16:27:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his176ala' Tue Jun 30 18:14:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His176Ala' P13747:p.Asp183Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:58:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp183ala' Tue Jun 30 16:42:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp183Ala' P13747:p.Glu187Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Wed May 02 17:05:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu187ala' Tue Jun 30 17:59:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu187Ala' P13747:p.Thr235Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T A Wed May 02 15:52:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr235ala' Tue Jun 30 20:14:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr235Ala' ecd sufficient to bind sufficient binding region sufficient to bind range range range range P13747:p.Arg83Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:11:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg83ala' Tue Jun 30 15:38:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg83Ala' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 51 33 35 54 56 physical association physical association Fig 4A, Table 2 HLA-E∗0103 allele klrc1-b2m-2 2 7 unspecified role unspecified role ancillary ancillary leader_sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait Q13241:p.Gln79Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 19:29:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln79ala' Tue Jun 30 20:07:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln79Ala' Q13241:p.Gln112Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 19:59:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln112ala' Tue Jun 30 16:22:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln112Ala' Q13241:p.Phe114Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:25:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe114ala' Tue Jun 30 17:08:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe114Ala' Q13241:p.Thr146Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T A Wed May 02 17:17:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr146ala' Tue Jun 30 18:33:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr146Ala' Q13241:p.Asn148Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N A Wed May 02 15:31:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn148ala' Tue Jun 30 16:09:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn148Ala' Q13241:p.Asn158Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N A Wed May 02 20:31:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn158ala' Tue Jun 30 16:50:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn158Ala' Q13241:p.Asn160Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N A Wed May 02 18:50:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn160ala' Tue Jun 30 17:52:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn160Ala' Q13241:p.Leu162Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 16:27:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu162ala' Tue Jun 30 17:07:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu162Ala' Q13241:p.Asp163Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:27:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp163ala' Tue Jun 30 19:35:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp163Ala' Q13241:p.Glu164Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 14:53:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu164ala' Tue Jun 30 18:14:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu164Ala' Q13241:p.Asp168Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Wed May 02 16:14:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp168ala' Tue Jun 30 20:02:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp168Ala' ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 59 63 61 65 78 physical association physical association Fig 4B, Table 3-1 HLA-E∗0103 allele klrc1-b2m-3 2 7 unspecified role unspecified role ancillary ancillary leader_sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role bait bait P26715:p.Ser172Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:58:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser172ala' Tue Jun 30 16:36:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser172Ala' P26715:p.Asp200Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Wed May 02 15:10:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp200ala' Tue Jun 30 16:20:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp200Ala' P26715:p.Asp202Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Wed May 02 14:48:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp202ala' Tue Jun 30 19:54:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp202Ala' P26715:p.Gln212Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 18:33:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln212ala' Tue Jun 30 18:03:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln212Ala' P26715:p.Val213Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain V A Wed May 02 14:34:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val213ala' Tue Jun 30 17:29:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val213Ala' P26715:p.Arg215Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 20:17:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg215ala' Tue Jun 30 19:50:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg215Ala' P26715:p.Lys217Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Wed May 02 14:54:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys217ala' Tue Jun 30 19:45:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys217Ala' P26715:p.Gln220Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 15:20:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln220ala' Tue Jun 30 17:02:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln220Ala' P26715:p.Ser223Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:53:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser223ala' Tue Jun 30 18:15:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser223Ala' P26715:p.Met163Ile mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain M I Wed May 02 20:33:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met163ile' Tue Jun 30 19:14:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met163Ile' P26715:p.Ser167_Ser170delinsAlaSerIleLeu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain SIIS ASIL Tue May 09 10:46:18 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ser_ile_ile_ser167-170' Wed May 02 15:21:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser_ile_ile_ser167-170ala_ser_ile_leu' Tue Jun 30 17:12:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser167_Ser170delinsAlaSerIleLeu' P26715:p.Arg137Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Wed May 02 14:56:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg137ala' Tue Jun 30 17:58:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg137Ala' ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 83 81 85 99 101 physical association physical association Fig 4B, Table 3-2 HLA-E∗0103 allele klrc1-b2m-4 2 3 unspecified role unspecified role bait bait P26717:p.Ala165_Leu168delinsSerIleIleSer mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain ASIL SIIS Tue May 09 10:54:23 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'ala_ser_ile_ser165-168ser_ile_ile_ser' Wed May 02 19:28:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala_ser_ile_leu165-168ser_ile_ile_ser' Tue Jun 30 16:45:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala165_Leu168delinsSerIleIleSer' ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role ancillary ancillary leader_sequence sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role prey prey whole_molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey ecd sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait ecd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 105 113 107 111 109 physical association physical association Fig 4B, Table 3-3 HLA-E∗0103 allele It is stated in the text:- "we replaced residues 161 and residues 165–168 with the corresponding amino acids from NKG2A" - but the corresponding residue in NKG2A is also an "M" not an "I". however Figure 3A gives an I/M residue difference at residues 225/223 for NKG2A/C respectively. Mutation I161M:- there is no other "I" residue around this location apart from the one in the ASIL motif - but I dont think they are referring to that one (residue 167) so I have NOT entered any I161M mutation.