DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Monomeric myosin V uses two binding regions for the assembly of stable translocation complexes. Monomeric myosin V uses two binding regions for the assembly of stable translocation complexes. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Heuck A., Du TG., Jellbauer S., Richter K., Kruse C., Jaklin S., Müller M., Buchner J., Jansen RP., Niessing D. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Heuck A., Du TG., Jellbauer S., Richter K., Kruse C., Jaklin S., Müller M., Buchner J., Jansen RP., Niessing D. DIP original experiment: DIP-68763X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Monomeric myosin V uses two binding regions for the assembly of stable translocation complexes. Monomeric myosin V uses two binding regions for the assembly of stable translocation complexes. Monomeric myosin V uses two binding regions for the assembly of stable translocation complexes. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Heuck A., Du TG., Jellbauer S., Richter K., Kruse C., Jaklin S., Müller M., Buchner J., Jansen RP., Niessing D. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Heuck A., Du TG., Jellbauer S., Richter K., Kruse C., Jaklin S., Müller M., Buchner J., Jansen RP., Niessing D. DIP original experiment: DIP-68762X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx f.3, not clear for me, there are some additional interactors. Please check it out. Monomeric myosin V uses two binding regions for the assembly of stable translocation complexes. Monomeric myosin V uses two binding regions for the assembly of stable translocation complexes. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Heuck A., Du TG., Jellbauer S., Richter K., Kruse C., Jaklin S., Müller M., Buchner J., Jansen RP., Niessing D. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Heuck A., Du TG., Jellbauer S., Richter K., Kruse C., Jaklin S., Müller M., Buchner J., Jansen RP., Niessing D. DIP original experiment: DIP-68761X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx f.1 she3_yeast SWI5-dependent HO expression protein 3 YBR1005 YBR130C SHE3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDQDNTQTSSSKLAPHHNIFMANLESSPTKDRNTSSQNASSSRVIESLHDQIDMLTKTNLQLTTQSQNLLSKLELAQSKESKLLENLNLLKNENENLNSIFERKNKKLKELEKDYSELSNRYNEQKEKMDQLSKLAKNSSAIEQSCSEKLQNMEVNYNSLLESQNLYRDHYSDEISKLNEKIGLLELELSNQNLNYGSDTSSNSDIELNLNKFNDSVKDLKSLETEKDSKLSKIITHSLDELNLQSWLNLYQTNENLISTFAEKMDLKDVLKRNDEKISNKGAVVQTLKKNVQTQVESNNADALSSNNAQDMLPIKMVKLRKTPNTNDSSSNGNSSNNKRRSFYTASPLLSSGSIPKSASPVLPGVKRTASVRKPSSSSSKTNVTHNNDPSTSPTISVPPGVTRTVSSTHKKKGNSMVVHGAQS 590404D2E140BB7A 590404D2E140BB7A myo4_yeast Myosin-4 MYO4 SHE1 YAL029C FUN22 SWI5-dependent HO expression protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFEVGTKCWYPHKEQGWIGGEVTKNDFFEGTFHLELKLEDGETVSIETNSFENDDDHPTLPVLRNPPILESTDDLTTLSYLNEPAVLHAIKKRYMNGQIYTYSGIVLIAANPFDKVDHLYSREMIQNYSSKRKDELEPHLFAIAEEAYRFMVHEKANQTVVVSGESGAGKTVSAKYIMRYFASVQESNNREGEVEMSQIESQILATNPIMEAFGNAKTTRNDNSSRFGKYLQILFDENTTIRGSKIRTYLLEKSRLVYQPETERNYHIFYQILEGLPEPVKQELHLSSPKDYHYTNQGGQPNIAGIDEAREYKITTDALSLVGINHETQLGIFKILAGLLHIGNIEMKMTRNDASLSSEEQNLQIACELLGIDPFNFAKWIVKKQIVTRSEKIVTNLNYNQALIARDSVAKFIYSTLFDWLVDNINKTLYDPELDQQDHVFSFIGILDIYGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFSDNQPCIDLIENKLGILSLLDEESRLPSGSDESWASKLYSAFNKPPSNEVFSKPRFGQTKFIVSHYAVDVEYEVEGFIEKNRDSVSLGHLDVFKATTNPIFKQILDNRELRSDDAPEEQNTEKKIMIPARLSQKKPTLGSMFKKSLGELMAIINSTNVHYIRCIKPNSEKKPWEFDNLMVLSQLRACGVLETIRISCAGFPSRWTFDEFVQRYFLLTDYSLWSGILYNPDLPKEAIVNFCQSILDATISDSAKYQIGNTKIFFKAGMLAFLEKLRTNKMNEICIIIQKKIRARYYRLQYLQTMESIKKCQSQIRSLLVRTRVDHELKTRAAILLQTNIRALWKREYYRAAIGQIIKLQCTCKRKLILDSVNRKFMLMAAVIIQSYIRSYGHKTDYRTLKRSSILVQSAMRMQLARRRYIVLQKEVEERNIRASYGIGLLEEAIEFKNSFILNLEMLNDSYTRLTQLLQGDLSNIPSKQRQEYETIVNGYNDKISKLKTLQVEIMNTLNKKNALKERKKKQSSLIQSHMQSLAAIKGNKPSRLSDEVKSMKQELAFIENVIAQDFTTTYSANKNDKVKGLGIAGQQVKPKLVNVIRRESGNPDLLELLMDLNCYTLEVTEGYLKKVNVTEVNGDNVLGPIHVITTVVSSLVRNGLLIQSSKFISKVLLTVESIVMSLPKDETMLGGIFWLSNLSRLPAFAANQKTLYEANGGDEKDKLTLIYLNDLENETLKVFDKIYSTWLVKFMKHASAHIEIFDMVLNEKLFKNSGDEKFAKLFTFLNEFDAVLCKFQVVDSMHTKIFNDTLKYLNVMLFNDLITKCPALNWKYGYEVDRNIERLVSWFEPRIEDVRPNLIQIIQAVKILQLKISNLNEFKLLFDFWYALNPAQIQAILLKYKPANKGEAGVPNEILNYLANVIKRENLSLPGKMEIMLSAQFDSAKNHLRYDTSAITQNSNTEGLATVSKIIKLDRK E79C0FE72B041E95 E79C0FE72B041E95 myo4p-she3-1 2 5 unspecified role unspecified role bait bait GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 4 unspecified role unspecified role prey prey His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction myo4p-she3-3 3 5 unspecified role unspecified role unspecified role unspecified role 923-1471 923-1471 sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 4 unspecified role unspecified role unspecified role unspecified role 1-234 binding site 1-234 binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction she3-myo4p-1 1 5 unspecified role unspecified role prey prey HA tag HA tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 4 unspecified role unspecified role bait bait myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association