DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Mechanism of degradation of CPEB during Xenopus oocyte maturation. Mechanism of degradation of CPEB during Xenopus oocyte maturation. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Setoyama D., Yamashita M., Sagata N. Only protein-protein interactions xenla Xenopus laevis (African clawed frog) African clawed frog anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Setoyama D., Yamashita M., Sagata N. DIP original experiment: DIP-111340X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Mechanism of degradation of CPEB during Xenopus oocyte maturation. Mechanism of degradation of CPEB during Xenopus oocyte maturation. Mechanism of degradation of CPEB during Xenopus oocyte maturation. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Setoyama D., Yamashita M., Sagata N. Only protein-protein interactions xenla Xenopus laevis (African clawed frog) African clawed frog pull down pull down anti tag western anti tag western blot anti tag western Setoyama D., Yamashita M., Sagata N. DIP original experiment: DIP-111339X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx f.1, f.2 Mechanism of degradation of CPEB during Xenopus oocyte maturation. Mechanism of degradation of CPEB during Xenopus oocyte maturation. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Setoyama D., Yamashita M., Sagata N. Only protein-protein interactions xenla Xenopus laevis (African clawed frog) African clawed frog pull down pull down anti tag western anti tag western blot anti tag western Setoyama D., Yamashita M., Sagata N. DIP original experiment: DIP-111341X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx f.3 cpe1a_xenla Cytoplasmic polyadenylation element-binding protein 1-A 58 kDa CPE-binding protein cpeb1-a cpeb protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAFPLKDDLGRAKDCWGCPSDTPALSTCSNADIFRRINAMLDNSLDFTGVCTTPNTKGKCEHLQDYQDTEGPAASRMLFSTSHEPLPRGLPDTNDLCLGLQSLSLTGWDRPWSTQDSEAGGHSSTPTAAQSVFSMLNSPMGKPSPLGFLTFDPIGSDLMEKYPTPLLRSSRLDSRSILDSRSSSPSDSDTSGFSSGSDHLSDLISSLRISPPLPFLPLGGGVSRDPLKLGIGSRLDQDHAALAAATVSPLGITKGWPSTSVWPSWDLLDSAEDPFSIEREARLHRQAAAVNEATCTWSGQLPPRNYKNPVYSCKVFLGGVPWDITETGLINTFRVFGALSVEWPGKDGKHPRCPPKGNMPKGYVYLVFESEKSVRALLQACSQDLLSQDGLSEHYFKMSSRRMRCKEVQVIPWVLADSNFVRSPSQRLDPSKTVFVGALHGMLNAEALASIMNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVSAAFVEIKTAKFTKKVQIDPYLEDSVCQVCNAQPGPFFCRDQVCFKYFCRSCWHWQHSMEILRHHRPLMRNQKSRDSS EF812949F5B4DE54 EF812949F5B4DE54 trcb_xenla Beta-TrCP fbxw1 btrcp Beta-transducin repeat-containing protein protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MEGFSCSLQPPTASEREDCNRDEPPRKIITEKNTLRQTKLANGTSSMIVPKQRKLSANYEKEKELCVKYFEQWSECDQVEFVEHLISRMCHYQHGHINTYLKPMLQRDFITALPARGLDHIAENILSYLDAKSLCSAELVCKEWYRVTSDGMLWKKLIERMVRTDSLWRGLAERRGWGQYLFKNKPPDGKTPPNSFYRALYPKIIQDIETIESNWRCGRHSLQRIHCRSETSKGVYCLQYDDQKIVSGLRDNTIKIWDKNTLECKRVLMGHTGSVLCLQYDERVIITGSSDSTVRVWDVNTGEMLNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMASATDITLRRVLVGHRAAVNVVDFDDKYIVSASGDRTIKVWNTSTCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNTIRLWDIECGACLRVLEGHEELVRCIRFDNKRIVSGAYDGKIKVWDLVAALDPRAPAGTLCLRTLVEHSGRVFRLQFDEFQIVSSSHDDTILIWDFLNDPGLA DIP protein Q91854 original sequence version: 57 Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins. May participate in Wnt signaling mint 2A52EC19028127F3 plk1_xenla Serine/threonine-protein kinase PLK1 plx1 Plx1 Polo-like kinase 1 plk1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAQVAGKKLTVAPEAAKPPGIPGSSSAVKEIPEILVDPRTRRRYLRGRFLGKGGFAKCYEITDLESREVFAGKIVPKTMLLKPHQKDKMTMEIAIQRSLDHRHVVGFHGFFEDNDFVYVVLELCRRRSLLELHKRRKAVTEPEARYYLKQTISGCQYLHSNRVIHRDLKLGNLFLNDEMEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLGKKGHSFEVDIWSIGCIMYTLLVGKPPFETSCLKETYMRIKKNEYSIPKHINPVAAALIQKMLRSDPTSRPTIDDLLNDEFFTSGYIPSRLPTTCLTVPPRFSIAPSTIDQSLRKPLTAINKGQDSPLVEKQVAPAKEEEMQQPEFTEPADCYLSEMLQQLTCLNAVKPSERALIRQEEAEDPASIPIFWISKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLIMYNDGDSLQYIERNNTESYLNVRSYPTTLTKKITLLKYFRNYMSEHLLKAGANTTPREGDELARLPFLRTWFRTRSAIILHLSNGTVQINFFQDHTKIILCPLMAAVSYIDEKREFRTYKLSLIQEFGCCKELASRLRYARTMVEKLQSSKSAVAHVKASA DIP protein P70032 original sequence version: 107 DIP protein P70032 original sequence version: 61 2467195911F225E6 cpeb-plk1-1 3 4 unspecified role unspecified role bait bait GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q91572:p.Thr125Ala T125A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'T125A mutation'. Wed May 02 18:32:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr125ala' Tue Jun 30 18:03:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr125Ala' xenla Xenopus laevis (African clawed frog) African clawed frog 6 unspecified role unspecified role prey prey xenla Xenopus laevis (African clawed frog) African clawed frog physical association physical association cpeb-fbxw1-1 2 5 unspecified role unspecified role prey prey myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range xenla Xenopus laevis (African clawed frog) African clawed frog 4 unspecified role unspecified role bait bait GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q91572:p.Gly192Ala G192A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain G A This feature has been corrected as a result of our quality control procedures. The original label was 'G192A mutation'. Wed May 02 15:31:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly192ala' Tue Jun 30 16:16:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly192Ala' xenla Xenopus laevis (African clawed frog) African clawed frog physical association physical association fbxw1-cpeb-1 1 5 unspecified role unspecified role bait bait myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range xenla Xenopus laevis (African clawed frog) African clawed frog 4 unspecified role unspecified role prey prey xenla Xenopus laevis (African clawed frog) African clawed frog physical association physical association