IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Molecular cell 2007 imex curation Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions ecoli Escherichia coli (strain K12) crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) Cross linking agent was EGS -ethylene glycol bis (succinimidylsuccinate) E_Alnemri@lac.jci.tju.edu Accepted 2017-MAY-31 AT 11:45 BST AT 11:45 BST by ORCHARD Molecular cell 2007 imex curation I have added 2 "domains" - but there is no InterPro XRef for the CC domain.; EBI-14036327 :- I have removed the mutation from one of the PSTPIP1 mols.- one without GST tag. The fully WT one is meant to be in (EBI-14036313) ; have added "mutation with no effect" to mutations, and deleted the 2 duplicate interactions. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Molecular cell 2007 imex curation Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu Accepted 2017-MAY-31 AT 11:45 BST AT 11:45 BST by ORCHARD Molecular cell 2007 imex curation I have added 2 "domains" - but there is no InterPro XRef for the CC domain.; EBI-14036327 :- I have removed the mutation from one of the PSTPIP1 mols.- one without GST tag. The fully WT one is meant to be in (EBI-14036313) ; have added "mutation with no effect" to mutations, and deleted the 2 duplicate interactions. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Molecular cell 2007 imex curation Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu Accepted 2017-MAY-31 AT 11:45 BST AT 11:45 BST by ORCHARD Molecular cell 2007 imex curation I have added 2 "domains" - but there is no InterPro XRef for the CC domain.; EBI-14036327 :- I have removed the mutation from one of the PSTPIP1 mols.- one without GST tag. The fully WT one is meant to be in (EBI-14036313) ; have added "mutation with no effect" to mutations, and deleted the 2 duplicate interactions. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Molecular cell 2007 imex curation Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu Accepted 2017-MAY-31 AT 11:45 BST AT 11:45 BST by ORCHARD Molecular cell 2007 imex curation I have added 2 "domains" - but there is no InterPro XRef for the CC domain.; EBI-14036327 :- I have removed the mutation from one of the PSTPIP1 mols.- one without GST tag. The fully WT one is meant to be in (EBI-14036313) ; have added "mutation with no effect" to mutations, and deleted the 2 duplicate interactions. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Molecular cell 2007 imex curation Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column anti tag western anti tag western blot anti tag western Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu Accepted 2017-MAY-31 AT 11:45 BST AT 11:45 BST by ORCHARD Molecular cell 2007 imex curation I have added 2 "domains" - but there is no InterPro XRef for the CC domain.; EBI-14036327 :- I have removed the mutation from one of the PSTPIP1 mols.- one without GST tag. The fully WT one is meant to be in (EBI-14036313) ; have added "mutation with no effect" to mutations, and deleted the 2 duplicate interactions. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Molecular cell 2007 imex curation Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions ecoli Escherichia coli (strain K12) crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) I have not added Cross linking for cell lysates as the presence of other proteins could give the MWt values detected;however text states the E.coli protein is "purified" but NO details are given for this purification in the Methods section. Cross linking agent was EGS -ethylene glycol bis (succinimidylsuccinate) E_Alnemri@lac.jci.tju.edu Accepted 2017-MAY-31 AT 11:45 BST AT 11:45 BST by ORCHARD Molecular cell 2007 imex curation I have added 2 "domains" - but there is no InterPro XRef for the CC domain.; EBI-14036327 :- I have removed the mutation from one of the PSTPIP1 mols.- one without GST tag. The fully WT one is meant to be in (EBI-14036313) ; have added "mutation with no effect" to mutations, and deleted the 2 duplicate interactions. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Pyrin activates the ASC pyroptosome in response to engagement by autoinflammatory PSTPIP1 mutants. Molecular cell 2007 imex curation Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yu JW., Fernandes-Alnemri T., Datta P., Wu J., Juliana C., Solorzano L., McCormick M., Zhang Z., Alnemri ES. Results obtained using a chimeric Trim5A and Pyrin molecule have not been included as they are of NO biological relevance. (Fig 5) E_Alnemri@lac.jci.tju.edu Accepted 2017-MAY-31 AT 11:45 BST AT 11:45 BST by ORCHARD Molecular cell 2007 imex curation I have added 2 "domains" - but there is no InterPro XRef for the CC domain.; EBI-14036327 :- I have removed the mutation from one of the PSTPIP1 mols.- one without GST tag. The fully WT one is meant to be in (EBI-14036313) ; have added "mutation with no effect" to mutations, and deleted the 2 duplicate interactions. ppip1_human Proline-serine-threonine phosphatase-interacting protein 1 CD2-binding protein 1 H-PIP PSTPIP1 CD2BP1 protein protein human Homo sapiens Human MMPQLQFKDAFWCRDFTAHTGYEVLLQRLLDGRKMCKDMEELLRQRAQAEERYGKELVQIARKAGGQTEINSLRASFDSLKQQMENVGSSHIQLALTLREELRSLEEFRERQKEQRKKYEAVMDRVQKSKLSLYKKAMESKKTYEQKCRDADDAEQAFERISANGHQKQVEKSQNKARQCKDSATEAERVYRQSIAQLEKVRAEWEQEHRTTCEAFQLQEFDRLTILRNALWVHSNQLSMQCVKDDELYEEVRLTLEGCSIDADIDSFIQAKSTGTEPPAPVPYQNYYDREVTPLTSSPGIQPSCGMIKRFSGLLHGSPKTTSLAASAASTETLTPTPERNEGVYTAIAVQEIQGNPASPAQEYRALYDYTAQNPDELDLSAGDILEVILEGEDGWWTVERNGQRGFVPGSYLEKL 97818150B3D5D600 mefv_human Pyrin MEFV MEF Marenostrin TRIM20 protein protein human Homo sapiens Human MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIQEYSTQENGTDDSAASSSLGENKPRSLKTPDHPEGNEGNGPRPYGGGAASLRCSQPEAGRGLSRKPLSKRREKASEGLDAQGKPRTRSPALPGGRSPGPCRALEGGQAEVRLRRNASSAGRLQGLAGGAPGQKECRPFEVYLPSGKMRPRSLEVTISTGEKAPANPEILLTLEEKTAANLDSATEPRARPTPDGGASADLKEGPGNPEHSVTGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSPGCPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHKKKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQMVGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLLHQKSEFVEKSTKYFSETLRSEMEMFNVPELIGAQAHAVNVILDAETAYPNLIFSDDLKSVRLGNKWERLPDGPQRFDSCIIVLGSPSFLSGRRYWEVEVGDKTAWILGACKTSISRKGNMTLSPENGYWVVIMMKENEYQASSVPPTRLLIKEPPKRVGIFVDYRVGSISFYNVTARSHIYTFASCSFSGPLQPIFSPGTRDGGKNTAPLTICPVGGQGPD DIP protein O15553 original sequence version: 151 Defects in MEFV are the cause of familial Mediterranean fever autosomal recessive (ARFMF) [MIM:249100]. ARFMF is an inherited disorder characterized by recurrent episodic fever, serosal inflammation and pain in the abdomen, chest or joints. ARFMF is frequently complicated by amyloidosis, which leads to renal failure and can be prophylactically treated with colchicine. ARFMF primarily affects ancestral ethnic groups living around the Mediterranean basin: North African Jews, Armenians, Arabs and Turks. The disease is also distributed in other populations including Greeks, Cypriots, Italians and Spanish, although at a lower prevalence Probably controls the inflammatory response in myelomonocytic cells at the level of the cytoskeleton organization mint Defects in MEFV are the cause of familial Mediterranean fever autosomal dominant (ADFMF) [MIM:134610]. ADFMF is characterized by periodic fever, serosal inflammation and pain in the abdomen, chest or joints as seen also in the autosomal recessive form of the disease. It is associated with renal amyloidosis and characterized by colchicine unresponsiveness 3692E5E6E9FC8204 asc_human Apoptosis-associated speck-like protein containing a CARD PYD and CARD domain-containing protein Target of methylation-induced silencing 1 Caspase recruitment domain-containing protein 5 ASC CARD5 TMS1 PYCARD protein protein human Homo sapiens Human MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS DIP protein Q9ULZ3 original sequence version: 116 455987286586F46A 365t34634-1 2 9 unspecified role unspecified role bait bait c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role prey prey physical association physical association 3E PSTPIP1, (WT)A230T and E250Q mutants. The interaction between pyrin and ASC was enhanced by coexpression of pyrin with WT PSTPIP1 and further enhanced by coexpression with the disease-associated PSTPIP1 mutants. mefv-4 1 9 unspecified role unspecified role neutral component neutral component purified purified coiled_coil_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n_terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 4 B/C, Supp 1B mefv-5 6 9 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain cc_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 4 A/C mefv-pstpip1-1 3 9 unspecified role unspecified role bait bait c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range b_box required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey O43586:p.Glu250Gln mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 16:02:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu250gln' Tue Jun 30 19:43:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu250Gln' c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range O43586:p.Ala230Thr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain A T Wed May 02 14:44:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala230thr' Tue Jun 30 19:43:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala230Thr' physical association physical association 5 D/E/F It is not easy to work out when they use the chimera and when not. I think 5 D/E do not use the chimera at all, and 5F does use it - but not for full length Pyrin. mefv-mefv-2 4 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined b_box required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 5G mefv-mefv-3 4 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined b_box required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined pyd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 inhibitor inhibitor unspecified role unspecified role O43586:p.Ala230Thr mutation mutation certain certain sequence position certain certain certain sequence position certain A T Wed May 02 17:59:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala230thr' Tue Jun 30 18:14:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala230Thr' c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) physical association physical association 5G-2 pstpip1-11 1 8 unspecified role unspecified role neutral component neutral component c-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp 1B pstpip1-pstpip1-1 7 8 unspecified role unspecified role prey prey c-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp 1C pstpip1-10 5 8 unspecified role unspecified role neutral component neutral component O43586:p.Ala230Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A T Wed May 02 18:48:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala230thr' Tue Jun 30 16:04:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala230Thr' c-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp 1Dii pstpip1-pstpip1-3 5 8 unspecified role unspecified role neutral component neutral component c-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O43586:p.Ala230Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A T Wed May 02 14:55:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala230thr' Tue Jun 30 18:47:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala230Thr' 8 unspecified role unspecified role neutral component neutral component c-terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O43586:p.Ala230Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A T Wed May 02 14:43:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala230thr' Tue Jun 30 17:58:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala230Thr' physical association physical association Supp 1Diii 3taester-1 5 9 unspecified role unspecified role neutral component neutral component LN-BB-CC_regions sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain n_terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp 1E mefv-1 5 9 unspecified role unspecified role neutral component neutral component LN-BB-CC_regions sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain n_terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role neutral component neutral component LN-BB-CC_regions sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain n_terminus t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp 1Ei mefv-pstpip1-2 4 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O43586:p.Ala230Thr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain A T Wed May 02 15:36:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala230thr' Tue Jun 30 16:09:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala230Thr' in vitro In vitro physical association physical association Supp 4 A/B mefv-pstpip1-3 4 9 unspecified role unspecified role bait bait c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O43586:p.Ala230Thr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain A T Wed May 02 15:14:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala230thr' Tue Jun 30 15:47:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala230Thr' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro association association Supp 4B