DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. DIP original experiment: DIP-261771X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. DIP original experiment: DIP-261769X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Scherr N., Honnappa S., Kunz G., Mueller P., Jayachandran R., Winkler F., Pieters J., Steinmetz MO. DIP original experiment: DIP-261770X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural basis for the specific inhibition of protein kinase G, a virulence factor of Mycobacterium tuberculosis. pkng_myctu Serine/threonine-protein kinase PknG MTCY22G10.06c Rv0410c pknG protein protein myctu Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) MAKASETERSGPGTQPADAQTATSATVRPLSTQAVFRPDFGDEDNFPHPTLGPDTEPQDRMATTSRVRPPVRRLGGGLVEIPRAPDIDPLEALMTNPVVPESKRFCWNCGRPVGRSDSETKGASEGWCPYCGSPYSFLPQLNPGDIVAGQYEVKGCIAHGGLGWIYLALDRNVNGRPVVLKGLVHSGDAEAQAMAMAERQFLAEVVHPSIVQIFNFVEHTDRHGDPVGYIVMEYVGGQSLKRSKGQKLPVAEAIAYLLEILPALSYLHSIGLVYNDLKPENIMLTEEQLKLIDLGAVSRINSFGYLYGTPGFQAPEIVRTGPTVATDIYTVGRTLAALTLDLPTRNGRYVDGLPEDDPVLKTYDSYGRLLRRAIDPDPRQRFTTAEEMSAQLTGVLREVVAQDTGVPRPGLSTIFSPSRSTFGVDLLVAHTDVYLDGQVHAEKLTANEIVTALSVPLVDPTDVAASVLQATVLSQPVQTLDSLRAARHGALDADGVDFSESVELPLMEVRALLDLGDVAKATRKLDDLAERVGWRWRLVWYRAVAELLTGDYDSATKHFTEVLDTFPGELAPKLALAATAELAGNTDEHKFYQTVWSTNDGVISAAFGLARARSAEGDRVGAVRTLDEVPPTSRHFTTARLTSAVTLLSGRSTSEVTEEQIRDAARRVEALPPTEPRVLQIRALVLGGALDWLKDNKASTNHILGFPFTSHGLRLGVEASLRSLARVAPTQRHRYTLVDMANKVRPTSTF DIP protein P9WI73 original sequence version: 2 DIP protein P9WI73 original sequence version: 14 CFC6E54DBE19569F pkng-1 2 4 unspecified role unspecified role unspecified role unspecified role Region 74-750 Region 74-750 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction pkng-pkng-1 3 4 enzyme target enzyme target substrate substrate unspecified role unspecified role P9WI73:p.Lys181Met K181M mutation mutation certain certain sequence position certain certain certain sequence position certain K M This feature has been corrected as a result of our quality control procedures. The original label was 'K181M'. Wed May 02 14:40:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys181met' Tue Jun 30 19:07:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys181Met' ecolx Escherichia coli 4 enzyme enzyme unspecified role unspecified role Region 74-750 Region 74-750 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation pkng-2 1 4 putative self putative self unspecified role unspecified role P9WI73:p.[Cys106Ser;Cys109Ser;Cys128Ser;Cys131Ser] C106/109/128/131S mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 18:43:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys106ser,cys109ser,cys128ser,cys131ser' Tue Jun 30 19:19:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys106Ser;Cys109Ser;Cys128Ser;Cys131Ser]' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation true