DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Nature structural & molecular biology 2007 imex curation Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66832X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Nature structural & molecular biology 2007 imex curation Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66830X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Nature structural & molecular biology 2007 imex curation Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66828X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Nature structural & molecular biology 2007 imex curation Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66831X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Nature structural & molecular biology 2007 imex curation Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66829X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. Nature structural & molecular biology 2007 imex curation Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Schwarz-Romond T., Fiedler M., Shibata N., Butler PJ., Kikuchi A., Higuchi Y., Bienz M. DIP original experiment: DIP-66827X Nature structural & molecular biology 2007 IMEx The DIX domain of Dishevelled confers Wnt signaling by dynamic polymerization. axin1_rat Axin-1 Axin1 Axin Axis inhibition protein 1 protein protein rat Rattus norvegicus (Rat) Rat MNVQEQGFPLDLGASFTEDAPRPPVPGEEGELVSTDSRPVNHSFCSGKGTSIKSETSTATPRRSDLDLGYEPEGSASPTPPYLRWAESLHSLLDDQDGISLFRTFLKQEGCADLLDFWFACSGFRKLEPCDSNEEKRLKLARAIYRKYILDSNGIVSRQTKPATKSFIKDCVMKQQIDPAMFDQAQTEIQSTMEENTYPSFLKSDIYLEYTRTGSESPKVCSDQSSGSGTGKGMSGYLPTLNEDEEWKCDQDADEDDGRDSVPPSRLTQKLLLETAAPRAPSSRRYNEGRELRYGSWREPVNPYYVNSGYALAPATSANDSEQQSLSSDADTLSLTDSSVDGIPPYRIRKQHRREMQESVQVNGRVPLPHIPRTYRMPKEIRVEPQKFAEELIHRLEAVQRTREAEEKLEERLKRVRMEEEGEDGEMPSGPMASHKLPSVPAWHHFPPRYVDMGCSGLRDAHEENPESILDEHVQRVMRTPGCQSPGPGHRSPDSGHVAKTAVLGGTASGHGKHAPKLGLKLDSAGLHHHRHVHHHVHHNSARPKEQMEAEAARRVQSSFSWGPETHGHAKPRSYSESTGTNPSAGDLAFGGKASAPSKRNTKKAESGKNASAEVPSTTEDAEKNQKIMQWIIEGEKEISRHRKAGHGSSGMRKQQAHESSRPLSIERPGAVHPWVSAQLRNSVQPSHLFIQDPTMPPNPAPNPLTQLEEARRRLEEEEKRANKLPSKQRTKSQRKAGGGSAPPCDSIVVAYYFCGEPIPYRTLVRGRAVTLGQFKELLTKKGSYRYYFKKVSDEFDCGVVFEEVREDEAILPVFEEKIIGKVEKVD BB1EE9CCECF6D487 BB1EE9CCECF6D487 dvl2_mouse Segment polarity protein dishevelled homolog DVL-2 Dvl2 DSH homolog 2 protein protein mouse Mus musculus Mouse MAGSSAGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDTPQPEVAPPAHESRTELVPPPPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRDRPRRRDSSEHGAGGHRPGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEDDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRMERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGAFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHMDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESELSSRGGSLRRGGEPGGTGDGGPPPSRGSTGAPPNLRALPGLHPYGAPSGMALPYNPMMVVMMPPPPPPVSTAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM DIP protein Q60838 original sequence version: 132 DIP protein Q60838 original sequence version: 63 DIP protein Q60838 original sequence version: 125 DIP protein Q60838 original sequence version: 69 DIP protein Q60838 original sequence version: 91 928A527143BBBBB0 dvl2-1 4 8 unspecified role unspecified role neutral component neutral component 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain Q60838:p.Tyr27Asp Y27D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y D This feature has been corrected as a result of our quality control procedures. The original label was 'Y27D mutation'. Wed May 02 15:25:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr27asp' Tue Jun 30 18:09:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr27Asp' Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F S This feature has been corrected as a result of our quality control procedures. The original label was 'F43S mutation'. Wed May 02 16:04:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe43ser' Tue Jun 30 19:07:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe43Ser' Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V67A K68A mutation'. Wed May 02 18:11:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val67ala,lys68ala' Tue Jun 30 15:53:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val67Ala;Lys68Ala]' ecolx Escherichia coli direct interaction direct interaction raxn1-1 1 7 unspecified role unspecified role unspecified role unspecified role 713-832 binding region 713-832 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction dvl2-dvl2-7 6 8 unspecified role unspecified role prey prey 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain Q60838:p.Tyr27Asp Y27D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y D This feature has been corrected as a result of our quality control procedures. The original label was 'Y27D mutation'. Wed May 02 14:53:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr27asp' Tue Jun 30 17:21:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr27Asp' Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F S This feature has been corrected as a result of our quality control procedures. The original label was 'F43S mutation'. Wed May 02 15:47:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe43ser' Tue Jun 30 20:12:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe43Ser' Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V67A K68A mutation'. Wed May 02 18:33:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val67ala,lys68ala' Tue Jun 30 17:10:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val67Ala;Lys68Ala]' in vitro In vitro 8 unspecified role unspecified role bait bait 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q60838:p.Tyr27Asp Y27D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y D This feature has been corrected as a result of our quality control procedures. The original label was 'Y27D mutation'. Wed May 02 15:26:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr27asp' Tue Jun 30 16:13:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr27Asp' Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F S This feature has been corrected as a result of our quality control procedures. The original label was 'F43S mutation'. Wed May 02 15:20:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe43ser' Tue Jun 30 17:28:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe43Ser' Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V67A K68A mutation'. Wed May 02 19:01:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val67ala,lys68ala' Tue Jun 30 16:45:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val67Ala;Lys68Ala]' ecolx Escherichia coli direct interaction direct interaction dvl2-dvl2-8 3 8 unspecified role unspecified role prey prey 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F S This feature has been corrected as a result of our quality control procedures. The original label was 'F43S mutation'. Wed May 02 15:08:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe43ser' Tue Jun 30 18:27:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe43Ser' Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V67A K68A mutation'. Wed May 02 15:00:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val67ala,lys68ala' Tue Jun 30 18:57:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val67Ala;Lys68Ala]' HA Tag HA Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 8 unspecified role unspecified role bait bait FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V67A K68A mutation'. Wed May 02 20:14:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val67ala,lys68ala' Tue Jun 30 17:42:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val67Ala;Lys68Ala]' Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F S This feature has been corrected as a result of our quality control procedures. The original label was 'F43S mutation'. Wed May 02 14:27:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe43ser' Tue Jun 30 20:19:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe43Ser' human Homo sapiens Human physical association physical association dvl2-2 5 8 unspecified role unspecified role neutral component neutral component 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain Q60838:p.Tyr27Asp Y27D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y D This feature has been corrected as a result of our quality control procedures. The original label was 'Y27D mutation'. Wed May 02 15:37:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr27asp' Tue Jun 30 15:34:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr27Asp' Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F S This feature has been corrected as a result of our quality control procedures. The original label was 'F43S mutation'. Wed May 02 15:18:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe43ser' Tue Jun 30 15:54:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe43Ser' Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V67A K68A mutation'. Wed May 02 14:29:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val67ala,lys68ala' Tue Jun 30 15:37:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val67Ala;Lys68Ala]' ecolx Escherichia coli direct interaction direct interaction dvl2-3 2 8 unspecified role unspecified role neutral component neutral component 1-114 binding region 1-114 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain Q60838:p.Tyr27Asp Y27D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y D This feature has been corrected as a result of our quality control procedures. The original label was 'Y27D mutation'. Wed May 02 19:10:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr27asp' Tue Jun 30 19:28:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr27Asp' Q60838:p.Phe43Ser F43S mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F S This feature has been corrected as a result of our quality control procedures. The original label was 'F43S mutation'. Wed May 02 17:29:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe43ser' Tue Jun 30 17:46:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe43Ser' Q60838:p.[Val67Ala;Lys68Ala] V67A K68A mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V67A K68A mutation'. Wed May 02 17:29:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val67ala,lys68ala' Tue Jun 30 18:43:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val67Ala;Lys68Ala]' ecolx Escherichia coli direct interaction direct interaction