DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261481X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261477X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261480X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261482X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261479X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhao G., Zhou X., Wang L., Li G., Schindelin H., Lennarz WJ. DIP original experiment: DIP-261478X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Studies on peptide:N-glycanase-p97 interaction suggest that p97 phosphorylation modulates endoplasmic reticulum-associated degradation. ngly1_mouse Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase Ngly1 N-glycanase 1 Peptide:N-glycanase protein protein mouse Mus musculus Mouse MASATLGSSSSSASPAVAELCQNTPETFLEASKLLLTYADNILRNPSDEKYRSIRIGNTAFSTRLLPVRGAVECLFEMGFEEGETHLIFPKKASVEQLQKIRDLIAIERSSRLDGSSKKVQFSQHPAAAKLPLEQSEDPAGLIRHSGNQTGQLPSLPSAPMVVGDSTILKVLQSNIQHVQLYENPVLQEKALTCIPVSELKRKAQEKLFRARKLDKGTNVSDEDFLLLELLHWFKEEFFRWVNNIVCSKCGGETRSRDEALLPNDDELKWGAKNVENHYCDACQLSNRFPRYNNPEKLLETRCGRCGEWANCFTLCCRALGFEARYVWDYTDHVWTEVYSPSQQRWLHCDACEDVCDKPLLYEIGWGKKLSYIIAFSKDEVVDVTWRYSCKHDEVMSRRTKVKEELLRETINGLNKQRQLSLSESRRKELLQRIIVELVEFISPKTPRPGELGGRVSGSLAWRVARGETGLERKEILFIPSENEKISKQFHLRYDIVRDRYIRVSDNNINISGWENGVWKMESIFRKVEKDWNMVYLARKEGSSFAYISWKFECGSAGLKVDTVSIRTSSQSFESGSVRWKLRSETAQVNLLGDKNLRSYNDFSGATEVTLEAELSRGDGDVAWQHTQLFRQSLNDSGENGLEIIITFNDL DIP protein Q9JI78 original sequence version: 87 F9D7A35726482F81 tera_mouse Transitional endoplasmic reticulum ATPase Vcp 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein mouse Mus musculus Mouse MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG DIP protein Q01853 original sequence version: 84 DIP protein Q01853 original sequence version: 112 DIP protein Q01853 original sequence version: 152 DIP protein Q01853 original sequence version: 136 DIP protein Q01853 original sequence version: 156 DIP protein Q01853 original sequence version: 149 501B721D3A77BA8A ngly1-vcp-3 2 7 unspecified role unspecified role unspecified role unspecified role Region 12-111 Region 12-111 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 801-806 Region 801-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction ngly1-vcp-4 1 7 unspecified role unspecified role unspecified role unspecified role Q9JI78:p.Arg55Glu R55E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R55E'. Wed May 02 15:54:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg55glu' Tue Jun 30 19:16:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg55Glu' ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ngly1-vcp-5 3 7 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 1-130 Region 1-130 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 8 unspecified role unspecified role prey prey Q01853:p.Tyr805Glu Y805E/F mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y E Wed May 02 15:12:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr805glu' Tue Jun 30 15:56:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr805Glu' His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q01853:p.Tyr805Phe mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Y F Wed May 02 16:08:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr805phe' Tue Jun 30 17:09:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr805Phe' ecolx Escherichia coli direct interaction direct interaction ngly1-vcp-6 4 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 767-806 Region 767-806 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ngly1-vcp-7 6 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ngly1-vcp-8 5 7 unspecified role unspecified role unspecified role unspecified role Q9JI78:p.Arg55Glu R55E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R E This feature has been corrected as a result of our quality control procedures. The original label was 'R55E'. Wed May 02 18:12:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg55glu' Tue Jun 30 19:47:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg55Glu' Q9JI78:p.Asn58Asp N58D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain N D This feature has been corrected as a result of our quality control procedures. The original label was 'N58D'. Wed May 02 16:48:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn58asp' Tue Jun 30 19:52:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn58Asp' ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Region 797-806 Region 797-806 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pY805 pY805 optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm in vitro In vitro direct interaction direct interaction