DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. Structure (London, England : 1993) 2007 imex curation Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. DIP original experiment: DIP-66885X Structure (London, England : 1993) 2007 IMEx NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. Structure (London, England : 1993) 2007 imex curation Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. DIP original experiment: DIP-66887X Structure (London, England : 1993) 2007 IMEx NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. Structure (London, England : 1993) 2007 imex curation Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Morales B., Ramirez-Espain X., Shaw AZ., Martin-Malpartida P., Yraola F., Sánchez-Tilló E., Farrera C., Celada A., Royo M., Macias MJ. DIP original experiment: DIP-66886X Structure (London, England : 1993) 2007 IMEx NMR structural studies of the ItchWW3 domain reveal that phosphorylation at T30 inhibits the interaction with PPxY-containing ligands. py peptide Latent membrane protein 2 [EEPPPPYED] peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EEPPPPYED pypeptide Latent membrane protein 2 [SNEEPPPPYEDPYWGN] peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SNEEPPPPYEDPYWGN itch_mouse E3 ubiquitin-protein ligase Itchy Itch HECT-type E3 ubiquitin transferase Itchy homolog protein protein mouse Mus musculus Mouse MSDSGPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPTSKLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSESTTQNDDGCRTRDDTRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNTSTSEGATSGLIIPLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE DIP protein Q8C863 original sequence version: 44 905FDBE00A1EA7EA itch-itch-1 1 5 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 5 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 6 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction itch-itch-2 3 5 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 5 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 6 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction itch-itch-3 2 4 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 4 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 6 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role WW3 domain 399-432 WW3 domain 399-432 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction