DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Benson MD., Li QJ., Kieckhafer K., Dudek D., Whorton MR., Sunahara RK., Iñiguez-Lluhí JA., Martens JR. Only protein-protein interactions chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey pull down pull down anti tag western anti tag western blot anti tag western Benson MD., Li QJ., Kieckhafer K., Dudek D., Whorton MR., Sunahara RK., Iñiguez-Lluhí JA., Martens JR. DIP original experiment: DIP-261000X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Benson MD., Li QJ., Kieckhafer K., Dudek D., Whorton MR., Sunahara RK., Iñiguez-Lluhí JA., Martens JR. Only protein-protein interactions chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey pull down pull down anti tag western anti tag western blot anti tag western Benson MD., Li QJ., Kieckhafer K., Dudek D., Whorton MR., Sunahara RK., Iñiguez-Lluhí JA., Martens JR. DIP original experiment: DIP-260999X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Benson MD., Li QJ., Kieckhafer K., Dudek D., Whorton MR., Sunahara RK., Iñiguez-Lluhí JA., Martens JR. Only protein-protein interactions chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey pull down pull down anti tag western anti tag western blot anti tag western Benson MD., Li QJ., Kieckhafer K., Dudek D., Whorton MR., Sunahara RK., Iñiguez-Lluhí JA., Martens JR. DIP original experiment: DIP-260997X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Benson MD., Li QJ., Kieckhafer K., Dudek D., Whorton MR., Sunahara RK., Iñiguez-Lluhí JA., Martens JR. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Benson MD., Li QJ., Kieckhafer K., Dudek D., Whorton MR., Sunahara RK., Iñiguez-Lluhí JA., Martens JR. DIP original experiment: DIP-260996X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Benson MD., Li QJ., Kieckhafer K., Dudek D., Whorton MR., Sunahara RK., Iñiguez-Lluhí JA., Martens JR. Only protein-protein interactions chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey pull down pull down anti tag western anti tag western blot anti tag western Benson MD., Li QJ., Kieckhafer K., Dudek D., Whorton MR., Sunahara RK., Iñiguez-Lluhí JA., Martens JR. DIP original experiment: DIP-260998X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx SUMO modification regulates inactivation of the voltage-gated potassium channel Kv1.5. sumo1_human Small ubiquitin-related modifier 1 SUMO1 Sentrin Ubiquitin-like protein SMT3C Ubiquitin-homology domain protein PIC1 Ubiquitin-like protein UBL1 GAP-modifying protein 1 SMT3 homolog 3 SMT3C SMT3H3 UBL1 OK/SW-cl.43 protein protein human Homo sapiens Human MSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTHLKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQEQTGGHSTV DIP protein P63165 original sequence version: 36 DIP protein P63165 original sequence version: 40 DIP protein P63165 original sequence version: 124 DIP protein P63165 original sequence version: 125 DIP protein P63165 original sequence version: 83 DIP protein P63165 original sequence version: 85 DIP protein P63165 original sequence version: 81 DIP protein P63165 original sequence version: 84 DIP protein P63165 original sequence version: 103 DIP protein P63165 original sequence version: 100 DIP protein P63165 original sequence version: 95 DIP protein P63165 original sequence version: 98 DIP protein P63165 original sequence version: 101 DIP protein P63165-1 original sequence version: 131 DIP protein P63165 original sequence version: 102 DIP protein P63165 original sequence version: 122 DIP protein P63165 original sequence version: 62 DIP protein P63165 original sequence version: 119 DIP protein P63165 original sequence version: 82 89BE97D2D054FB33 sumo3_human Small ubiquitin-related modifier 3 SMT3 homolog 1 SUMO-2 SMT3H1 SUMO3 SMT3A Ubiquitin-like protein SMT3A protein protein human Homo sapiens Human MSEEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVPESSLAGHSF DIP protein P55854 original sequence version: 56 DIP protein P55854 original sequence version: 127 DIP protein P55854 original sequence version: 106 DIP protein P55854 original sequence version: 124 DIP protein P55854 original sequence version: 99 E337E34CB5B3B187 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG sumo2_human Small ubiquitin-related modifier 2 SMT3 homolog 2 Sentrin-2 HSMT3 SUMO-3 SUMO2 SMT3H2 SMT3B Ubiquitin-like protein SMT3B protein protein human Homo sapiens Human MADEKPKEGVKTENNDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYCERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGGVY DIP protein P61956 original sequence version: 39 DIP protein P61956 original sequence version: 79 DIP protein P61956 original sequence version: 81 DIP protein P61956 original sequence version: 77 DIP protein P61956 original sequence version: 98 DIP protein P61956 original sequence version: 116 DIP protein P61956 original sequence version: 141 F8F0426849BEF08B kcna5_human Potassium voltage-gated channel subfamily A member 5 KCNA5 HPCN1 Voltage-gated potassium channel HK2 Voltage-gated potassium channel subunit Kv1.5 protein protein human Homo sapiens Human MEIALVPLENGGAMTVRGGDEARAGCGQATGGELQCPPTAGLSDGPKEPAPKGRGAQRDADSGVRPLPPLPDPGVRPLPPLPEELPRPRRPPPEDEEEEGDPGLGTVEDQALGTASLHHQRVHINISGLRFETQLGTLAQFPNTLLGDPAKRLRYFDPLRNEYFFDRNRPSFDGILYYYQSGGRLRRPVNVSLDVFADEIRFYQLGDEAMERFREDEGFIKEEEKPLPRNEFQRQVWLIFEYPESSGSARAIAIVSVLVILISIITFCLETLPEFRDERELLRHPPAPHQPPAPAPGANGSGVMAPPSGPTVAPLLPRTLADPFFIVETTCVIWFTFELLVRFFACPSKAGFSRNIMNIIDVVAIFPYFITLGTELAEQQPGGGGGGQNGQQAMSLAILRVIRLVRVFRIFKLSRHSKGLQILGKTLQASMRELGLLIFFLFIGVILFSSAVYFAEADNQGTHFSSIPDAFWWAVVTMTTVGYGDMRPITVGGKIVGSLCAIAGVLTIALPVPVIVSNFNYFYHRETDHEEPAVLKEEQGTQSQGPGLDRGVQRKVSGSRGSFCKAGGTLENADSARRGSCPLEKCNVKAKSNVDLRRSLYALCLDTSRETDL DIP protein P22460 original sequence version: 104 DIP protein P22460-1 original sequence version: 138 A5B02B27F8396E3D ubc9_human SUMO-conjugating enzyme UBC9 UBE2I p18 SUMO-protein ligase UBC9 UBCE9 Ubiquitin carrier protein 9 Ubiquitin carrier protein I Ubiquitin-conjugating enzyme E2 I Ubiquitin-protein ligase I RING-type E3 SUMO transferase UBC9 protein protein human Homo sapiens Human MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS DIP protein P63279 original sequence version: 33 DIP protein P63279 original sequence version: 57 DIP protein P63279 original sequence version: 80 DIP protein P63279 original sequence version: 137 DIP protein P63279 original sequence version: 99 DIP protein P63279 original sequence version: 140 DIP protein P63279 original sequence version: 120 DIP protein P63279 original sequence version: 117 DIP protein P63279 original sequence version: 93 E2C826E9C8D0683D ube2i-kcna5-1 4 10 unspecified role unspecified role prey prey Region 1-556 Region 1-556 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 11 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association kcna5-sumo1-1 5 10 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Tag used for western blot. His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey 6 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association kcna5-sumo3-1 1 10 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Tag used for western blot. His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey 7 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association kcna5-ub-1 3 10 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Tag used for western blot. His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey 8 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association kcna5-sumo2-1 2 10 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Tag used for western blot. His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey 9 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey physical association physical association