DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. Nature structural & molecular biology 2007 imex curation Reese ML., Dakoji S., Bredt DS., Dötsch V. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Reese ML., Dakoji S., Bredt DS., Dötsch V. DIP original experiment: DIP-66615X Nature structural & molecular biology 2007 IMEx The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. Nature structural & molecular biology 2007 imex curation Reese ML., Dakoji S., Bredt DS., Dötsch V. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Reese ML., Dakoji S., Bredt DS., Dötsch V. DIP original experiment: DIP-66617X Nature structural & molecular biology 2007 IMEx The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. Nature structural & molecular biology 2007 imex curation Reese ML., Dakoji S., Bredt DS., Dötsch V. Only protein-protein interactions in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Reese ML., Dakoji S., Bredt DS., Dötsch V. DIP original experiment: DIP-66616X Nature structural & molecular biology 2007 IMEx The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. Nature structural & molecular biology 2007 imex curation Reese ML., Dakoji S., Bredt DS., Dötsch V. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Reese ML., Dakoji S., Bredt DS., Dötsch V. DIP original experiment: DIP-66614X Nature structural & molecular biology 2007 IMEx The guanylate kinase domain of the MAGUK PSD-95 binds dynamically to a conserved motif in MAP1a. map1a_rat Microtubule-associated protein 1A Map1a Mtap1a protein protein rat Rattus norvegicus (Rat) Rat MDGVAEFSEYVSETVDVPSPFDLLEPPTSGGFLKLSKPCCYIFPGGRGDSALFAVNGFNILVDGGSDRKSCFWKLVRHLDRIDSVLLTHIGADNLPGINGLLQRKVAELEEEQSQGSSSYSDWVKNLISPELGVVFFNVPDKLRLPDASRKAKRSIEEACLTLQHLNRLGIQAEPLYRVVSNTIEPLTLFHKMGVGRLDMYVLNPVKDSKEMQFLMQKWAGNSKAKTGIVLANGKEAEISVPYLTSITALVVWLPANPTEKIVRVLFPGNAPQNKILEGLEKLRHLDFLRYPVATQKDLAAGAVPANLKPSKIKHRADSKESLKAAPKTAVSKLAKREEVLEEGAKEARSELAKELAKTEKKAKEPSEKPPEKPSKSERVRGESSEALKAEKRRLIKDKAGKKHLKEKISKLEEKKDKEKKEIKKERKELKKEEGRKEEKKDAKKDEKRKDTKPEVKKLSKPDLKPFTPEVRKTLYKAKAPGRVKVDKGRAARGEKELSSEPRTPPAQKGAAPPAAVSGHRELALSSPEDLTQDFEELKREERGLLAEQRDTGLGEKPLPADATEQGHPSAAIQVTQPSGPVLEGEHVEREKEVVPDSPGDKGSTNRGPDSGAEVEKEKETWEERKQREAELGPENTAAREESEAEVKEDVIEKAELEEMEETHPSDEEGEETKAESFYQKHTQEALKASPKSREALGGRDLGFQGKAPEKETASFLSSLATPAGATEHVSYIQDETIPGYSETEQTISDEEIHDEPDERPAPPRFPTSTYDLSGPEGPGPFEASQAADSAVPASSSKTYGAPETELTYPPNMVAAPLAEEEHVSSATSITECDKLSSFATSVAEDQSVASLTAPQTEETGKSSLLLDTVTSIPSSRTEATQGLDYVPSAGTISPTSSLEEDKGFKSPPCEDFSVTGESEKKGETVGRGLSGEKAVGKEEKYVVTSEKLSGQYAAVFGAPGHTLPPGEPALGEVEERCLSPDDSTVKMASPPPSGPPSAAHTPFHQSPVEDKSEPRDFQEDSWGETKHSPGVSKEDSEEQTVKPGPEEGTSEEGKGPPTRSPQAQDMPVSIAGGQTGCTIQLLPEQDKAIVFETGEAGSNLGAGTLPGEVRTSTEEATEPQKDEVLRFTDQSLSPEDAESLSVLSVVSPDTTKQEATPRSPCSLKEQQPHKDLWPMVSPEDTQSLSFSEESPSKETSLDISSKQLSPESLGTLQFGELNLGKEERGPVMKAEDDSCHLAPVSIPEPHRATVSPSTDETPAGTLPGGSFSHSALSVDRKHSPGEITGPGGHFMTSDSSLTKSPESLSSPAMEDLAVEWEGKAPGKEKEPELKSETRQQKGQILPEKVAVVEQDLIIHQKDGALDEENKPGRQQDKTPEQKGRDLDEKDTAAELDKGPEPKEKDLDREDQGQRAGPPAEKDKASEQRDTDLQQTQATEPRDRAQERRDSEEKDKSLELRDRTPEEKDRILVQEDRAPEHSIPEPTQTDRAPDRKGTDDKEQKEEASEEKEQVLEQKDWALGKEGETLDQEARTAEQKDETLKEDKTQGQKSSFVEDKTTTSKETVLDQKSAEKADSVEQQDGAALEKTRALGLEESPAEGSKAREQEKKYWKEQDVVQGWRETSPTRGEPVGGQKEPVPAWEGKSPEQEVRYWRDRDITLQQDAYWRELSCDRKVWFPHELDGQGARPRYCEERESTFLDEGPDEQEITPLQHTPRSPWTSDFKDFQEPLPQKGLEVERWLAESPVGLPPEEEDKLTRSPFEIISPPASPPEMTGQRVPSAPGQESPVPDTESTAPMRNEPTTPSWLAEIPPWVPKDRPLPPAPLSPAPAPPTPAPEPHTPVPFSWGLAEYDSVVAAVQEGAAELEGGPYSPLGKDYRKAEGEREGEGGAGAPDSSSFSPKVPEAGESLATRDTEQTEPEQREPTPYPDERSFQYADIYEQMMLTGLGPACPTREPPLGASGDWPPHLSTKEEAAGCNTSAEKETSSPASPQNLQSDTPAFSYASLAGPAVPPRQEPDPGPNVEPSITPPAVPPRAPISLSKDLSPPLNGSTVSCSPDRRTPSPKETGRGHWDDGTNDSDLEKGAREQPEKETRSPSPHHPMPMGHSSLWPETEAYSSLSSDSHLGSVRPSLDFPASAFGFSSLQPAPPQLPSPAEPRSAPCGSLAFSGDRALALVPGTPTRTRHDEYLEVTKAPSLDSSLPQLPSPSSPGGPLLSNLPRPASPALSEGSSSEATTPVISSVAERFPPGLEAAEQSAEGLGSGKESAAHSLWDLTPLSPAPSASLDLAPAPAPAPAPAPGLPGDLGDGTLPCRPECTGELTKKPSPFLSPSGDHEANGPGETSLNPPGFVTATAEKEEAEAPHAWERGSWPEGAERSSRPDTLLSSEQPLRPGKSSGGPPCSLSSEVEAGPQGCATDPRPHCGELSPSFLNPPLPPSTDDSDLSTEEARLAGKGGRRRVGRPGATGGPCPMADETPPTSASDSGSSQSDSDVPPETEECPSITAEAALDSDEDGDFLPVDKAGGVSGTHHPRPGHDPPPTPLPDPRPSPPRPDVCMADPEGLSSESGRVERLREKGRPGRRAPGRAKPASPARRLDIRGKRSPTPGKGPVDRTSRTVPRPRSTPSQVTSAEEKDGHSPMSKGLVNGLKAGSTALGSKGGSGPPVYVDLAYIPNHCSGKTADQDFFRRVRASYYVVSGNDPANGEPSRAVLDALLEGKAQWGENLQVTLIPTHDTEVTREWYQQTHEQQQQLNVLVLASSSTVVMQDESFPACKIEF DIP protein P34926 original sequence version: 52 3DEF74427BA9D7D7 dlg4_rat Disks large homolog 4 Dlg4 Dlgh4 Postsynaptic density protein 95 Synapse-associated protein 90 Psd95 protein protein rat Rattus norvegicus (Rat) Rat MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL DIP protein P31016 original sequence version: 86 DIP protein P31016-1 original sequence version: 148 7922D4E8E0F9AD85 map1a-dlg4-14 4 5 unspecified role unspecified role bait bait 1862-1878 binding region 1862-1878 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role prey prey SH3GK SH3GK sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction dlg4-map1a-1 1 5 unspecified role unspecified role neutral component neutral component 1862-1878 binding region 1862-1878 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role neutral component neutral component SH3GK SH3GK sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction map1a-dlg4-15 3 5 unspecified role unspecified role bait bait 1869-1878 binding region 1869-1878 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role prey prey SH3GK SH3GK sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction dlg4-map1a-2 2 6 unspecified role unspecified role neutral component neutral component SH3GK SH3GK sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role neutral component neutral component 1862-1878 binding region 1862-1878 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Rhodamine Tag Rhodamine Tag dye labelled dye label dye labelled predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro direct interaction direct interaction