DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. DIP original experiment: DIP-260561X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. DIP original experiment: DIP-260563X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. DIP original experiment: DIP-260559X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. DIP original experiment: DIP-260558X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. Only protein-protein interactions in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. DIP original experiment: DIP-260557X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. Only protein-protein interactions in vitro In vitro tem transmission electron microscopy tem predetermined predetermined participant predetermined Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. DIP original experiment: DIP-260562X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Structural insights into the p97-Ufd1-Npl4 complex. Proceedings of the National Academy of Sciences of the United States of America 2007 imex curation Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. Only protein-protein interactions in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Pye VE., Beuron F., Keetch CA., McKeown C., Robinson CV., Meyer HH., Zhang X., Freemont PS. DIP original experiment: DIP-260560X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Structural insights into the p97-Ufd1-Npl4 complex. ufd1_mouse Ubiquitin recognition factor in ER-associated degradation protein 1 Ufd1 Ubiquitin fusion degradation protein 1 homolog Ufd1l protein protein mouse Mus musculus Mouse MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERPVQHEESIEGEADHSGYAGEVGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKKVEEDEAGGRFIAFSGEGQSLRKKGRKP DIP protein P70362 original sequence version: 80 DIP protein P70362 original sequence version: 88 mint homomint Essential component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (By similarity). It may be involved in the development of some ectoderm-derived structures C85E06B1B910485D tera_mouse Transitional endoplasmic reticulum ATPase Vcp 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein mouse Mus musculus Mouse MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG DIP protein Q01853 original sequence version: 84 DIP protein Q01853 original sequence version: 112 DIP protein Q01853 original sequence version: 152 DIP protein Q01853 original sequence version: 136 DIP protein Q01853 original sequence version: 156 DIP protein Q01853 original sequence version: 149 501B721D3A77BA8A ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG C42A35397FFD9B52 npl4_rat Nuclear protein localization protein 4 homolog Nploc4 Npl4 protein protein rat Rattus norvegicus (Rat) Rat MAESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSSKSLHLLKIKHGDLLFLFPSSLAGPSSEMETSTSVGLKAFGAPHVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEVAAIYEPPQIGTQNSLELLEDPKAEVVDEIASKLGLRKVGWIFTDLVSEDTRKGTVRYSRNKDTYFLSSEECITAGDFQNKHPNICRLSPDGHFGSKFVTAVATGGPDNQVHFEGYQVSNQCMALVRDECLLPCKDAPELGYAKESSSEQYVPDVFYKDIDKFGNEITQLARPLPVEYLIIDITTTFPKDPVYTFSISQNPFPIENRDVLGETQDFHSLATYLSQNTSSVFLDTISDFHLLLFLVTNEVMPLQDSISLLLEAVRTRNEELAQTWKKSEQWATIEQLCSTVGVQLPGLHEFGAVGGSARAATSAMWACQHCTFMNQPGTGHCEMCSLPRT DIP protein Q9ES54 original sequence version: 87 DIP protein Q9ES54 original sequence version: 97 F6F57DDEA53594A3 nploc4-ufd1l-4 5 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction nploc4-ufd1l-5 6 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association nploc4-ufd1l-8 7 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction nploc4-ufd1l-9 4 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association nploc4-ufd1l-10 1 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction nploc4-ub-1 2 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association nploc4-ufd1l-11 3 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction