DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Paul I., Cui J., Maynard EL. Only protein-protein interactions in vitro In vitro light scattering light scattering weight by comassie molecular weight estimation by coomasie staining weight by comassie Paul I., Cui J., Maynard EL. DIP original experiment: DIP-264245X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Paul I., Cui J., Maynard EL. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Paul I., Cui J., Maynard EL. DIP original experiment: DIP-264244X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Paul I., Cui J., Maynard EL. Only protein-protein interactions ecolx Escherichia coli comigration in sds comigration in sds page comigration in sds weight by staining molecular weight estimation by staining weight by staining Paul I., Cui J., Maynard EL. DIP original experiment: DIP-264242X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Paul I., Cui J., Maynard EL. Only protein-protein interactions ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Paul I., Cui J., Maynard EL. DIP original experiment: DIP-264243X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Zinc binding to the HCCH motif of HIV-1 virion infectivity factor induces a conformational change that mediates protein-protein interactions. vif_hv1h2 Virion infectivity factor SOR protein vif protein protein hv1h2 Human immunodeficiency virus type 1 group M subtype B (isolate HXB2) (HIV-1) HIV-1 MENRWQVMIVWQVDRMRIRTWKSLVKHHMYVSGKARGWFYRHHYESPHPRISSEVHIPLGDARLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPELADQLIHLYYFDCFSDSAIRKALLGHIVSPRCEYQAGHNKVGSLQYLALAALITPKKIKPPLPSVTKLTEDRWNKPQKTKGHRGSHTMNGH DIP protein P69723 original sequence version: 74 DIP protein P69723 original sequence version: 72 vif-1 3 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction vif-2 4 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction vif-3 2 5 unspecified role unspecified role unspecified role unspecified role Region 101-142 Region 101-142 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction vif-4 1 5 unspecified role unspecified role unspecified role unspecified role Region 101-142 Region 101-142 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction