DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Nature 2006 imex curation Darieva Z., Bulmer R., Pic-Taylor A., Doris KS., Geymonat M., Sedgwick SG., Morgan BA., Sharrocks AD. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Darieva Z., Bulmer R., Pic-Taylor A., Doris KS., Geymonat M., Sedgwick SG., Morgan BA., Sharrocks AD. DIP original experiment: DIP-254831X Nature 2006 IMEx Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Nature 2006 imex curation Darieva Z., Bulmer R., Pic-Taylor A., Doris KS., Geymonat M., Sedgwick SG., Morgan BA., Sharrocks AD. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Darieva Z., Bulmer R., Pic-Taylor A., Doris KS., Geymonat M., Sedgwick SG., Morgan BA., Sharrocks AD. DIP original experiment: DIP-254832X Nature 2006 IMEx Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Nature 2006 imex curation Darieva Z., Bulmer R., Pic-Taylor A., Doris KS., Geymonat M., Sedgwick SG., Morgan BA., Sharrocks AD. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Darieva Z., Bulmer R., Pic-Taylor A., Doris KS., Geymonat M., Sedgwick SG., Morgan BA., Sharrocks AD. DIP original experiment: DIP-254833X Nature 2006 IMEx Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. Nature 2006 imex curation Darieva Z., Bulmer R., Pic-Taylor A., Doris KS., Geymonat M., Sedgwick SG., Morgan BA., Sharrocks AD. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Darieva Z., Bulmer R., Pic-Taylor A., Doris KS., Geymonat M., Sedgwick SG., Morgan BA., Sharrocks AD. DIP original experiment: DIP-254834X Nature 2006 IMEx Polo kinase controls cell-cycle-dependent transcription by targeting a coactivator protein. cdc5_yeast Cell cycle serine/threonine-protein kinase CDC5/MSD2 CDC5 MSD2 PKX2 YMR001C YM8270.03C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLGPLKAINDKQLNTRSKLVHTPIKGNTADLVGKENHFKQTKRLDPNNDHHHQPAQKKKREKLSALCKTPPSLIKTRGKDYHRGHFLGEGGFARCFQIKDDSGEIFAAKTVAKASIKSEKTRKKLLSEIQIHKSMSHPNIVQFIDCFEDDSNVYILLEICPNGSLMELLKRRKVLTEPEVRFFTTQICGAIKYMHSRRVIHRDLKLGNIFFDSNYNLKIGDFGLAAVLANESERKYTICGTPNYIAPEVLMGKHSGHSFEVDIWSLGVMLYALLIGKPPFQARDVNTIYERIKCRDFSFPRDKPISDEGKILIRDILSLDPIERPSLTEIMDYVWFRGTFPPSIPSTVMSEAPNFEDIPEEQSLVNFKDCMEKSLLLESMSSDKIQRQKRDYISSIKSSIDKLEEYHQNRPFLPHSLSPGGTKQKYKEVVDIEAQRRLNDLAREARIRRAQQAVLRKELIATSTNVIKSEISLRILASECHLTLNGIVEAEAQYKMGGLPKSRLPKIKHPMIVTKWVDYSNKHGFSYQLSTEDIGVLFNNGTTVLRLADAEEFWYISYDDREGWVASHYLLSEKPRELSRHLEVVDFFAKYMKANLSRVSTFGREEYHKDDVFLRRYTRYKPFVMFELSDGTFQFNFKDHHKMAISDGGKLVTYISPSHESTTYPLVEVLKYGEIPGYPESNFREKLTLIKEGLKQKSTIVTVD B5A25F1BBBEAA3DC B5A25F1BBBEAA3DC bfa1_yeast Mitotic check point protein BFA1 Cell cycle arrest protein BFA1 BFA1 YJR053W J1667 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSIRPLTLNGLDEPETSFEELNTTLPRFQSHETLTLEENVPPLSTSTYIPPPSSVGTSDTGTVFSNSTSAFWSNKQADDDQDMEVDQDDEFLNDFQEFQNKKDDFDDAIKTNFHLRNGCRTGPFKNDIFAEEFDRKLSLEDKPRLKQPRSMMELKPKRKLSNSVTSRNLRSGNSVRFKKSMPNLALVNPAIREEEEDEEREREDQREFNYKIDNDTQDTILAKFSSDDEGDFLTGFEELEGEAIDETISSNDKESADHPRFLKKSSSSLPLKISPAQYDIVKHDELLTPGLHRRQRDWNTQQELDSFKEKRSVRHCSNQNVQLNGPAKIKTIKQQIDHNTPMKKGSMIYNPKTMKWEGNENVLSKFSDVDTANRKALLIKNKLQRDADSKKQKYSDLQHARATSRNQKVIGNMILDEQNLRWVSVSEEEADPFAGIPEINLPPVGKSMKKRSSSPFLRSKSQVNTPFVSNDNDGVYQSTAAQARLRKYHSMRTLNGTTETPEISSTFHLSSRALEKFYHEENRWCKKLASWFIPRDETIISVDEETIMDESTVNSKRKSYMYEIRNMVINSTKD 1EA75D26FCF041DA 1EA75D26FCF041DA fkh2_yeast Fork head protein homolog 2 N2403 YNL2403C YNL068C FKH2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSNFNEMNELNMTQTNYGSTKYTAQHHQGVINAIISSLTAPDQPTTVSLQYSNDKNMATEIQAYAKLSGPNWTYYVKDLEVSIGRNTDPLNSALQENSDGVKNSYRVNIDLGPAKVVSRKHAIIKYNMNIGGWELHILGRNGAKVNFQRTHNGPNNPPIRLSSGTLLDIGGTQMMFILPDSDPVVAPICIEHLMPNLINMFGLEGNNNPLLRDIIKQSNYAKQRQLTSNQQIKGFKLYGSGGNAPFGSGANLGPSEQGIFNNNNNSKNKNGYFTSINPNYTASTTTSNTINPQAASPQGPPNTIIAANFVDSYKSSNAYPQALDFTSDLSHDENRNVKPPHSYATMITQAILSSPEGVISLADIYKYISSNYAYYRFAKSGWQNSIRHNLSLNKAFEKVPRRPNEPGKGMKWRISESYQQEFLNKWNTGKVGKIRRGSSVARQLQLHMAKFNSLPMEMDYRLSLNMAQPPKRQLQSHNVLEPSNNNIIEGFVQHVPSKGNLPASQQSQPPVSHQNQSQQPPPQEQRQEIQFTFADTQNRNIALARPIKTPQLQAPNSNANLNQNNMKEYKESLHPPAISISQMNRQSPNNALVSFTNACANSKIINNISDSADKSTNNNGGTKMNLPAISTSSLDENGNLEPTTTTSSGNSNSVPQTGTTTSSLAANSLRLSQPYDTLLRSPTKAFHITAMEAYTPERGSANRARSPLHSNSNNTNNNGANNSNLQTSGMENKQTGLVLDSNVLKSMESNNDNRRLTPSTSKSQNVKSSPGVWNLLQFSSTNNTPAADSGGNKRGFSINPDIKAKENENATSEKDSDSNSNDLETKDINSSPLKNQGGSTANAKELILDTDGAKISIINN 68A03F5EB7BB5CF3 68A03F5EB7BB5CF3 ndd1_yeast Nuclear division defective protein 1 NDD1 YOR372C protein protein yeast Saccharomyces cerevisiae Baker's yeast MDRDISYQQNYTSTGATATSSRQPSTDNNADTNFLKVMSEFKYNFNSPLPTTTQFPTPYSSNQYQQTQDHFANTDAHNSSSNESSLVENSILPHHQQIQQQQQQQQQQQQQQQALGSLVPPAVTRTDTSETLDDINVQPSSVLQFGNSLPSEFLVASPEQFKEFLLDSPSTNFNFFHKTPAKTPLRFVTDSNGAQQSTTENPGQQQNVFSNVDLNNLLKSNGKTPSSSCTGAFSRTPLSKIDMNLMFNQPLPTSPSKRFSSLSLTPYGRKILNDVGTPYAKALISSNSALVDFQKARKDITTNATSIGLENANNILQRTPLRSNNKKLFIKTPQDTINSTSTLTKDNENKQDIYGSSPTTIQLNSSITKSISKLDNSRIPLLASRSDNILDSNVDDQLFDLGLTRLPLSPTPNCNSLHSTTTGTSALQIPELPKMGSFRSDTGINPISSSNTVSFKSKSGNNNSKGRIKKNGKKPSKFQIIVANIDQFNQDTSSSSLSSSLNASSSAGNSNSNVTKKRASKLKRSQSLLSDSGSKSQARKSCNSKSNGNLFNSQ 1CF99115F2B1F23D 1CF99115F2B1F23D cdc5-fkh2p-1 1 7 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction bfa1p-cdc5-1 3 6 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli 5 enzyme enzyme unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast phosphorylation phosphorylation reaction phosphorylation cdc5-fkh2p-2 4 7 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 458-862 Region 458-862 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 enzyme enzyme unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast phosphorylation phosphorylation reaction phosphorylation cdc5-ndd1p-1 2 8 enzyme target enzyme target substrate substrate unspecified role unspecified role pS85 pS85 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations western blot western blot Immuno blot Immuno blot certain certain sequence position certain certain certain sequence position certain Q08887:p.Ser85Ala S85A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S85A'. Wed May 02 15:19:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser85ala' Tue Jun 30 20:06:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser85Ala' ecolx Escherichia coli 5 enzyme enzyme unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast phosphorylation phosphorylation reaction phosphorylation