DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Nature structural & molecular biology 2006 imex curation Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. DIP original experiment: DIP-66578X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Nature structural & molecular biology 2006 imex curation Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. DIP original experiment: DIP-66579X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. Nature structural & molecular biology 2006 imex curation Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Alam SL., Langelier C., Whitby FG., Koirala S., Robinson H., Hill CP., Sundquist WI. DIP original experiment: DIP-66577X Nature structural & molecular biology 2006 IMEx Structural basis for ubiquitin recognition by the human ESCRT-II EAP45 GLUE domain. vps36_human Vacuolar protein-sorting-associated protein 36 VPS36 C13orf9 EAP45 ELL-associated protein of 45 kDa ESCRT-II complex subunit VPS36 CGI-145 protein protein human Homo sapiens Human MDRFVWTSGLLEINETLVIQQRGVRIYDGEEKIKFDAGTLLLSTHRLIWRDQKNHECCMAILLSQIVFIEEQAAGIGKSAKIVVHLHPAPPNKEPGPFQSSKNSYIKLSFKEHGQIEFYRRLSEEMTQRRWENMPVSQSLQTNRGPQPGRIRAVGIVGIERKLEEKRKETDKNISEAFEDLSKLMIKAKEMVELSKSIANKIKDKQGDITEDETIRFKSYLLSMGIANPVTRETYGSGTQYHMQLAKQLAGILQVPLEERGGIMSLTEVYCLVNRARGMELLSPEDLVNACKMLEALKLPLRLRVFDSGVMVIELQSHKEEEMVASALETVSEKGSLTSEEFAKLVGMSVLLAKERLLLAEKMGHLCRDDSVEGLRFYPNLFMTQS DIP protein Q86VN1 original sequence version: 33 21E1E66F71BA7764 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG ub-vps36-1 3 5 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli 4 unspecified role unspecified role neutral component neutral component 1-139 binding region 1-139 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ub-vps36-2 2 5 unspecified role unspecified role bait bait GST Tag GST Tag experimental feature experimental feature predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal in vitro In vitro 4 unspecified role unspecified role prey prey 1-139 binding region 1-139 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction vps36-ub-1 1 5 unspecified role unspecified role prey prey ile44ala I44A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I44A mutation'. ecolx Escherichia coli 4 unspecified role unspecified role bait bait 1-139 binding region 1-139 binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain GST Tag GST Tag experimental feature experimental feature predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q86VN1:p.Val67Ala V67A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain V A This feature has been corrected as a result of our quality control procedures. The original label was 'V67A mutation'. Wed May 02 18:20:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val67ala' Tue Jun 30 17:26:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val67Ala' Q86VN1:p.Phe68Ala F68A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F68A mutation'. Wed May 02 17:24:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe68ala' Tue Jun 30 19:08:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe68Ala' Q86VN1:p.Glu70Ala E70A mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E70A mutation'. Wed May 02 16:43:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu70ala' Tue Jun 30 19:38:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu70Ala' ecolx Escherichia coli direct interaction direct interaction