DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Interplay between PIP3 and calmodulin regulation of olfactory cyclic nucleotide-gated channels. Interplay between PIP3 and calmodulin regulation of olfactory cyclic nucleotide-gated channels. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Brady JD., Rich ED., Martens JR., Karpen JW., Varnum MD., Brown RL. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Brady JD., Rich ED., Martens JR., Karpen JW., Varnum MD., Brown RL. DIP original experiment: DIP-263985X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Interplay between PIP3 and calmodulin regulation of olfactory cyclic nucleotide-gated channels. Interplay between PIP3 and calmodulin regulation of olfactory cyclic nucleotide-gated channels. Interplay between PIP3 and calmodulin regulation of olfactory cyclic nucleotide-gated channels. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Brady JD., Rich ED., Martens JR., Karpen JW., Varnum MD., Brown RL. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Brady JD., Rich ED., Martens JR., Karpen JW., Varnum MD., Brown RL. DIP original experiment: DIP-263986X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Interplay between PIP3 and calmodulin regulation of olfactory cyclic nucleotide-gated channels. Interplay between PIP3 and calmodulin regulation of olfactory cyclic nucleotide-gated channels. Interplay between PIP3 and calmodulin regulation of olfactory cyclic nucleotide-gated channels. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Brady JD., Rich ED., Martens JR., Karpen JW., Varnum MD., Brown RL. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting anti tag western anti tag western blot anti tag western Brady JD., Rich ED., Martens JR., Karpen JW., Varnum MD., Brown RL. DIP original experiment: DIP-263984X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Interplay between PIP3 and calmodulin regulation of olfactory cyclic nucleotide-gated channels. cngb1_bovin Cyclic nucleotide-gated cation channel beta-1 CNGB1 CNCG4 Cyclic nucleotide-gated channel beta-1 Cyclic nucleotide-gated cation channel 4 Cyclic nucleotide-gated cation channel modulatory subunit 240 kDa protein of rod photoreceptor CNG-channel Glutamic acid-rich protein Cyclic nucleotide-gated cation channel gamma protein protein bovin Bos taurus (Bovine) Bovine MLGWVQRVLPQPPGTPQKTKQEEEGTEPEPELEPKPETAPEETELEEVSLPPEEPCVGKEVAAVTLGPQGTQETALTPPTSLQAQVSVAPEAHSSPRGWVLTWLRKGVEKVVPQPAHSSRPSQNIAAGLESPDQQAGAQILGQCGTGGSDEPSEPSRAEDPGPGPWLLRWFEQNLEKMLPQPPKISEGWRDEPTDAALGPEPPGPALEIKPMLQAQESPSLPAPGPPEPEEEPIPEPQPTIQASSLPPPQDSARLMAWILHRLEMALPQPVIRGKGGEQESDAPVTCDVQTISILPGEQEESHLILEEVDPHWEEDEHQEGSTSTSPRTSEAAPADEEKGKVVEQTPRELPRIQEEKEDEEEEKEDGEEEEEEGREKEEEEGEEKEEEEGREKEEEEGEKKEEEGREKEEEEGGEKEDEEGREKEEEEGRGKEEEEGGEKEEEEGRGKEEVEGREEEEDEEEEQDHSVLLDSYLVPQSEEDRSEESETQDQSEVGGAQAQGEVGGAQALSEESETQDQSEVGGAQDQSEVGGAQAQGEVGGAQEQDGVGGAQDQSTSHQELQEEALADSSGVPATEEHPELQVEDADADSRPLIAEENPPSPVQLPLSPAKSDTLAVPGSATGSLRKRLPSQDDEAEELKMLSPAASPVVAWSDPTSPQGTDDQDRATSTASQNSAIINDRLQELVKLFKERTEKVKEKLIDPDVTSDEESPKPSPAKKAPEPAPEVKPAEAGQVEEEHYCEMLCCKFKRRPWKKYQFPQSIDPLTNLMYILWLFFVVLAWNWNCWLIPVRWAFPYQTPDNIHLWLLMDYLCDLIYLLDITVFQMRLQFVRGGDIITDKKEMRNNYVKSQRFKMDMLCLLPLDLLYLKFGVNPLLRLPRCLKYMAFFEFNNRLESILSKAYVYRVIRTTAYLLYSLHLNSCLYYWASAYEGLGSTHWVYDGVGNSYIRCYYWAVKTLITIGGLPDPRTLFEIVFQGLNYFTGVFAFSVMIGQMRDVVGAATAGQTYYRSCMDSTVKYMNFYKIPRSVQNRVKTWYEYTWHSQGMLDESELMVQLPDKMRLDLAIDVNYSIVSKVALFQGCDRQMIFDMLKRLRSVVYLPNDYVCKKGEIGREMYIIQAGQVQVLGGPDGKSVLVTLKAGSVFGEISLLAVGGGNRRTANVVAHGFTNLFILDKKDLNEILVHYPESQKLLRKKARRMLRNNNKPKEKSVLILPPRAGTPKLFNAALAAAGKMGAKGGRGGRLALLRARLKELAALEAAARQQQLLEQAKSSEDAAVGEEGSASPEQPPRPEPPAPEAPAPEPTAPEPLAPEAPAPEAPAPSSPPPASQERPEGDKDAARPEEHPVRIHVTLGPDPSEQILLVEVPEKQEEKEKKEEETEEKEEGEEARKEKEEE DIP protein Q28181 original sequence version: 104 mint EE6DA559BE3744A7 cnga2_rat Cyclic nucleotide-gated olfactory channel Cyclic nucleotide-gated cation channel 2 Cyclic nucleotide-gated channel alpha-2 Cyclic nucleotide-gated olfactory channel subunit OCNC1 Cnga2 Cncg2 protein protein rat Rattus norvegicus (Rat) Rat MMTEKSNGVKSSPANNHNHHPPPSIKANGKDDHRAGSRPQSVAADDDTSPELQRLAEMDTPRRGRGGFQRIVRLVGVIRDWANKNFREEEPRPDSFLERFRGPELQTVTTHQGDDKGGKDGEGKGTKKKFELFVLDPAGDWYYRWLFVIAMPVLYNWCLLVARACFSDLQRNYFVVWLVLDYFSDTVYIADLIIRLRTGFLEQGLLVKDPKKLRDNYIHTLQFKLDVASIIPTDLIYFAVGIHSPEVRFNRLLHFARMFEFFDRTETRTSYPNIFRISNLVLYILVIIHWNACIYYVISKSIGFGVDTWVYPNITDPEYGYLAREYIYCLYWSTLTLTTIGETPPPVKDEEYLFVIFDFLIGVLIFATIVGNVGSMISNMNATRAEFQAKIDAVKHYMQFRKVSKDMEAKVIKWFDYLWTNKKTVDEREVLKNLPAKLRAEIAINVHLSTLKKVRIFQDCEAGLLVELVLKLRPQVFSPGDYICRKGDIGKEMYIIKEGKLAVVADDGVTQYALLSAGSCFGEISILNIKGSKMGNRRTANIRSLGYSDLFCLSKDDLMEAVTEYPDAKKVLEERGREILMKEGLLDENEVAASMEVDVQEKLEQLETNMDTLYTRFARLLAEYTGAQQKLKQRITVLETKMKQNHEDDYLSDGINTPEPTAAE DIP protein Q00195 original sequence version: 111 cnga4_rat Cyclic nucleotide-gated cation channel alpha-4 Cyclic nucleotide-gated channel alpha-4 Cyclic nucleotide-gated olfactory channel subunit OCNC2 Cnga4 Cgn2 protein protein rat Rattus norvegicus (Rat) Rat MSQDGKVKTTESTPPAPTKARKWLPVLDPSGDYYYWWLNTMVFPIMYNLIIVVCRACFPDLQHSYLVAWFVLDYTSDLLYLLDIGVRFHTGFLEQGILVVDKGMIASRYVRTWSFLLDLASLVPTDAAYVQLGPHIPTLRLNRFLRVPRLFEAFDRTETRTAYPNAFRIAKLMLYIFVVIHWNSCLYFALSRYLGFGRDAWVYPDPAQPGFERLRRQYLYSFYFSTLILTTVGDTPLPDREEEYLFMVGDFLLAVMGFATIMGSMSSVIYNMNTADAAFYPDHALVKKYMKLQHVNKRLERRVIDWYQHLQINKKMTNEVAILQHLPERLRAEVAVSVHLSTLSRVQIFQNCEASLLEELVLKLQPQTYSPGEYVCRKGDIGREMYIIREGQLAVVADDGVTQYAVLGAGLYFGEISIINIKGNMSGNRRTANIKSLGYSDLFCLSKEDLREVLSEYPQAQAVMEEKGREILLKMNKLDVNAEAAEIALQEATESRLKGLDQQLDDLQTKFARLLAELESSALKIAYRIERLEWQTREWPMPEDMGEADDEAEPGEGTSKDGEGKAGQAGPSGIE DIP protein Q64359 original sequence version: 106 calm_human Calmodulin CALM1 CALM CAM CAM1 CALM2 CAMB CALM3 CAM3 CAMC CAMIII CAM2 CALML2 protein protein human Homo sapiens Human MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK DIP protein P62158 original sequence version: 97 DIP protein P62158 original sequence version: 144 DIP protein P62158 original sequence version: 146 DIP protein P62158 original sequence version: 73 DIP protein P62158 original sequence version: 102 DIP protein P62158 original sequence version: 98 DIP protein P62158 original sequence version: 96 DIP protein P62158 original sequence version: 111 DIP protein P62158 original sequence version: 131 DIP protein P62158 original sequence version: 136 DIP protein P62158 original sequence version: 145 DIP protein P62158 original sequence version: 154 DIP protein P62158 original sequence version: 148 DIP protein P62158 original sequence version: 116 DIP protein P62158 original sequence version: 120 DIP protein P62158 original sequence version: 130 6B4BC3FCDE10727B cnga2-calm1-1 3 7 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal Out of date range Feature range: Line: 375, Character: 10: It is not consistent to have a 'c-terminal' range position with a value different from the sequence length because 'c-terminal' means the last amino acid of the participant.However, we can accept a position null or 0 if the participant sequence is not known. Actual position : (0-0), sequence length: 149, It is not consistent to have a 'c-terminal' range position with a value different from the sequence length because 'c-terminal' means the last amino acid of the participant.However, we can accept a position null or 0 if the participant sequence is not known. Actual position : (0-0), sequence length: 149 ecolx Escherichia coli 5 unspecified role unspecified role bait bait Region 61-90 Region 61-90 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 1-138 Region 1-138 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cnga4-calm1-1 2 6 unspecified role unspecified role bait bait Region 271-339 Region 271-339 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal Out of date range Feature range: Line: 550, Character: 10: It is not consistent to have a 'c-terminal' range position with a value different from the sequence length because 'c-terminal' means the last amino acid of the participant.However, we can accept a position null or 0 if the participant sequence is not known. Actual position : (0-0), sequence length: 149, It is not consistent to have a 'c-terminal' range position with a value different from the sequence length because 'c-terminal' means the last amino acid of the participant.However, we can accept a position null or 0 if the participant sequence is not known. Actual position : (0-0), sequence length: 149 ecolx Escherichia coli direct interaction direct interaction cngb1-calm1-1 1 4 unspecified role unspecified role bait bait Region 677-764 Region 677-764 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal Out of date range Feature range: Line: 892, Character: 10: It is not consistent to have a 'c-terminal' range position with a value different from the sequence length because 'c-terminal' means the last amino acid of the participant.However, we can accept a position null or 0 if the participant sequence is not known. Actual position : (0-0), sequence length: 149, It is not consistent to have a 'c-terminal' range position with a value different from the sequence length because 'c-terminal' means the last amino acid of the participant.However, we can accept a position null or 0 if the participant sequence is not known. Actual position : (0-0), sequence length: 149 ecolx Escherichia coli direct interaction direct interaction