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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
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      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Regulation of intracellular free calcium concentration during heterocyst differentiation by HetR and NtcA in Anabaena sp. PCC 7120.</fullName>
        </names>
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            <attribute name="publication title" nameAc="MI:1091">Regulation of intracellular free calcium concentration during heterocyst differentiation by HetR and NtcA in Anabaena sp. PCC 7120.</attribute>
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            <attribute name="publication year" nameAc="MI:0886">2006</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
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        <participantIdentificationMethod>
          <names>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Shi Y., Zhao W., Zhang W., Ye Z., Zhao J.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-263427X</attribute>
          <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
          <attribute name="publication year" nameAc="MI:0886">2006</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
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          <fullName>Regulation of intracellular free calcium concentration during heterocyst differentiation by HetR and NtcA in Anabaena sp. PCC 7120.</fullName>
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            <names>
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        </hostOrganismList>
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            <shortLabel>protease assay</shortLabel>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Shi Y., Zhao W., Zhang W., Ye Z., Zhao J.</attribute>
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          <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
          <attribute name="publication year" nameAc="MI:0886">2006</attribute>
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      <experimentDescription id="3">
        <names>
          <fullName>Regulation of intracellular free calcium concentration during heterocyst differentiation by HetR and NtcA in Anabaena sp. PCC 7120.</fullName>
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        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-23775" refType="imex-primary" refTypeAc="MI:0662"/>
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        <hostOrganismList>
          <hostOrganism ncbiTaxId="562">
            <names>
              <shortLabel>ecolx</shortLabel>
              <fullName>Escherichia coli</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>comigration in sds</shortLabel>
            <fullName>comigration in sds page</fullName>
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          <xref>
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          <names>
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            <fullName>molecular weight estimation by staining</fullName>
            <alias type="synonym" typeAc="MI:1041">weight by staining</alias>
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          <xref>
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        </participantIdentificationMethod>
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          <attribute name="publication year" nameAc="MI:0886">2006</attribute>
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          <attribute name="title" nameAc="MI:1091">Regulation of intracellular free calcium concentration during heterocyst differentiation by HetR and NtcA in Anabaena sp. PCC 7120.</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="4">
        <names>
          <shortLabel>q8yy42_noss1</shortLabel>
          <alias type="gene name" typeAc="MI:0301">ccbP</alias>
          <alias type="locus name" typeAc="MI:0305">alr1010</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q8YY42" version="65" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="BAB72967" version="TrEMBL_107" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15592098" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="BAB72967" version="TrEMBL_107" refType="gene"/>
          <secondaryRef db="ensemblbacteria" id="BAB72967" version="TrEMBL_107" refType="transcript"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR020994" version="TrEMBL_107"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR043067" version="TrEMBL_107"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2K2V" version="TrEMBL_107"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2P0P" version="TrEMBL_107"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2P0Q" version="TrEMBL_107"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3URG" version="TrEMBL_107"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_010995184.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-61233N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="103690">
          <names>
            <shortLabel>noss1</shortLabel>
            <fullName>Nostoc sp. (strain PCC 7120 / UTEX 2576)</fullName>
          </names>
        </organism>
        <sequence>MASVERDETREHRIETEIIVDAEDKEERAMGWYYYLDDTLEFPFMGKWKKKSRKTSTIEEKTVEVLGMAPDDECLKDMYVEVADIGGKDDDVYTAKLSDIEAIDVDDDTQEAIADWLYWLARGYKF</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein Q8YY42 original sequence version: 65</attribute>
        </attributeList>
      </interactor>
      <interactor id="5">
        <names>
          <shortLabel>hetr_noss1</shortLabel>
          <fullName>DNA-binding transcriptional activator HetR</fullName>
          <alias type="gene name" typeAc="MI:0301">hetR</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Heterocyst differentiation control protein</alias>
          <alias type="locus name" typeAc="MI:0305">alr2339</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P27709" version="81" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="BAB74038" version="SP_107" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15592082" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="BAB74038" version="SP_107" refType="gene"/>
          <secondaryRef db="ensemblbacteria" id="BAB74038" version="SP_107" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0003677" version="SP_107"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0004252" version="SP_107"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="SP_107"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0043158" version="SP_107"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR005319" version="SP_107"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR040949" version="SP_107"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR041935" version="SP_107"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR041936" version="SP_107"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4HRI" version="SP_107"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4LH9" version="SP_107"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4YNL" version="SP_107"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="4YRV" version="SP_107"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_010996495.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-61232N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="103690">
          <names>
            <shortLabel>noss1</shortLabel>
            <fullName>Nostoc sp. (strain PCC 7120 / UTEX 2576)</fullName>
          </names>
        </organism>
        <sequence>MSNDIDLIKRLGPSAMDQIMLYLAFSAMRTSGHRHGAFLDAAATAAKCAIYMTYLEQGQNLRMTGHLHHLEPKRVKIIVEEVRQALMEGKLLKTLGSQEPRYLIQFPYVWMEQYPWIPGRSRIPGTSLTSEEKRQIEHKLPSNLPDAQLVTSFEFLELIEFLHKRSQEDLPPEHRMELSEALAEHIKRRLLYSGTVTRIDSPWGMPFYALTRPFYAPADDQERTYIMVEDTARYFRMMKDWAEKRPNAMRALEELDVPPERWDEAMQELDEIIRTWADKYHQVGGIPMILQMVFGRKED</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein P27709 original sequence version: 81</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="6" imexId="IM-23775-1">
        <names>
          <shortLabel>ccbp-hetr-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="dip" dbAc="MI:0465" id="DIP-197869E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15592112" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-23775-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="7">
            <interactorRef>4</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>enzyme target</shortLabel>
                <fullName>enzyme target</fullName>
                <alias type="go synonym" typeAc="MI:0303">substrate</alias>
                <alias type="synonym" typeAc="MI:1041">substrate</alias>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0502" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-64" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
          <participant id="8">
            <interactorRef>5</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>enzyme</shortLabel>
                <fullName>enzyme</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0501" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-46" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="9">
                <names>
                  <shortLabel>P27709:p.Ser152Ala</shortLabel>
                  <fullName>S152A</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-15592117" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation disrupting rate</shortLabel>
                    <fullName>mutation disrupting interaction rate</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation disrupting rate</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1129" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>alanine scanning</shortLabel>
                    <fullName>alanine scanning</fullName>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456759" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureDetectionMethod>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="152"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="152"/>
                    <resultingSequence>
                      <originalSequence>S</originalSequence>
                      <newSequence>A</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">This feature has been corrected as a result of our quality control procedures. The original label was 'S152A'.</attribute>
                  <attribute name="remark-internal">Wed May 02 16:00:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser152ala'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 18:25:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser152Ala'</attribute>
                </attributeList>
              </feature>
              <feature id="10">
                <names>
                  <shortLabel>P27709:p.Ser179Asn</shortLabel>
                  <fullName>S179N</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-15592120" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation disrupting rate</shortLabel>
                    <fullName>mutation disrupting interaction rate</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation disrupting rate</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1129" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>substitut analysis</shortLabel>
                    <fullName>substitution analysis</fullName>
                    <alias type="synonym" typeAc="MI:1041">substitut analysis</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0810" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1218575" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureDetectionMethod>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="179"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="179"/>
                    <resultingSequence>
                      <originalSequence>S</originalSequence>
                      <newSequence>N</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">This feature has been corrected as a result of our quality control procedures. The original label was 'S179N'.</attribute>
                  <attribute name="remark-internal">Wed May 02 14:56:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser179asn'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 18:30:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser179Asn'</attribute>
                </attributeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>protein cleavage</shortLabel>
            <fullName>protein cleavage</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0570" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-705088" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14744292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
      </interaction>
      <interaction id="11" imexId="IM-23775-2">
        <names>
          <shortLabel>hetr-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="dip" dbAc="MI:0465" id="DIP-197870E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15592135" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-23775-2" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>2</experimentRef>
        </experimentList>
        <participantList>
          <participant id="12">
            <interactorRef>5</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>putative self</shortLabel>
                <fullName>putative self</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0898" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813295" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="13">
                <names>
                  <shortLabel>P27709:p.Ser152Ala</shortLabel>
                  <fullName>S152A</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-15592140" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation disrupting rate</shortLabel>
                    <fullName>mutation disrupting interaction rate</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation disrupting rate</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1129" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>alanine scanning</shortLabel>
                    <fullName>alanine scanning</fullName>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456759" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureDetectionMethod>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="152"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="152"/>
                    <resultingSequence>
                      <originalSequence>S</originalSequence>
                      <newSequence>A</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">This feature has been corrected as a result of our quality control procedures. The original label was 'S152A'.</attribute>
                  <attribute name="remark-internal">Wed May 02 17:01:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser152ala'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 17:28:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser152Ala'</attribute>
                </attributeList>
              </feature>
              <feature id="14">
                <names>
                  <shortLabel>P27709:p.Ser179Asn</shortLabel>
                  <fullName>S179N</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-15592143" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation disrupting rate</shortLabel>
                    <fullName>mutation disrupting interaction rate</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation disrupting rate</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1129" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>substitut analysis</shortLabel>
                    <fullName>substitution analysis</fullName>
                    <alias type="synonym" typeAc="MI:1041">substitut analysis</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0810" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1218575" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureDetectionMethod>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="179"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="179"/>
                    <resultingSequence>
                      <originalSequence>S</originalSequence>
                      <newSequence>N</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">This feature has been corrected as a result of our quality control procedures. The original label was 'S179N'.</attribute>
                  <attribute name="remark-internal">Wed May 02 17:06:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser179asn'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 17:57:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser179Asn'</attribute>
                </attributeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>protein cleavage</shortLabel>
            <fullName>protein cleavage</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0570" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-705088" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14744292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <intraMolecular>true</intraMolecular>
      </interaction>
      <interaction id="15" imexId="IM-23775-3">
        <names>
          <shortLabel>hetr-2</shortLabel>
        </names>
        <xref>
          <primaryRef db="dip" dbAc="MI:0465" id="DIP-197870E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15592157" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-23775-3" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>3</experimentRef>
        </experimentList>
        <participantList>
          <participant id="16">
            <interactorRef>5</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>putative self</shortLabel>
                <fullName>putative self</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0898" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1813295" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <featureList>
              <feature id="17">
                <names>
                  <shortLabel>P27709:p.Ser152Ala</shortLabel>
                  <fullName>S152A</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-15592162" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation disrupting rate</shortLabel>
                    <fullName>mutation disrupting interaction rate</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation disrupting rate</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1129" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>alanine scanning</shortLabel>
                    <fullName>alanine scanning</fullName>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-456759" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureDetectionMethod>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="152"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="152"/>
                    <resultingSequence>
                      <originalSequence>S</originalSequence>
                      <newSequence>A</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">This feature has been corrected as a result of our quality control procedures. The original label was 'S152A'.</attribute>
                  <attribute name="remark-internal">Wed May 02 17:38:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser152ala'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 18:44:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser152Ala'</attribute>
                </attributeList>
              </feature>
              <feature id="18">
                <names>
                  <shortLabel>P27709:p.Ser179Asn</shortLabel>
                  <fullName>S179N</fullName>
                </names>
                <xref>
                  <primaryRef db="intact" dbAc="MI:0469" id="EBI-15592165" refType="identity" refTypeAc="MI:0356"/>
                </xref>
                <featureType>
                  <names>
                    <shortLabel>mutation disrupting rate</shortLabel>
                    <fullName>mutation disrupting interaction rate</fullName>
                    <alias type="synonym" typeAc="MI:1041">mutation disrupting rate</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:1129" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-5528005" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureType>
                <featureDetectionMethod>
                  <names>
                    <shortLabel>substitut analysis</shortLabel>
                    <fullName>substitution analysis</fullName>
                    <alias type="synonym" typeAc="MI:1041">substitut analysis</alias>
                  </names>
                  <xref>
                    <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0810" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1218575" refType="identity" refTypeAc="MI:0356"/>
                    <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                  </xref>
                </featureDetectionMethod>
                <featureRangeList>
                  <featureRange>
                    <startStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </startStatus>
                    <begin position="179"/>
                    <endStatus>
                      <names>
                        <shortLabel>certain</shortLabel>
                        <fullName>certain sequence position</fullName>
                        <alias type="synonym" typeAc="MI:1041">certain</alias>
                      </names>
                      <xref>
                        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0335" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-540564" refType="identity" refTypeAc="MI:0356"/>
                        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                      </xref>
                    </endStatus>
                    <end position="179"/>
                    <resultingSequence>
                      <originalSequence>S</originalSequence>
                      <newSequence>N</newSequence>
                    </resultingSequence>
                  </featureRange>
                </featureRangeList>
                <attributeList>
                  <attribute name="remark-internal">This feature has been corrected as a result of our quality control procedures. The original label was 'S179N'.</attribute>
                  <attribute name="remark-internal">Wed May 02 15:32:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser179asn'</attribute>
                  <attribute name="remark-internal">Tue Jun 30 15:52:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser179Asn'</attribute>
                </attributeList>
              </feature>
            </featureList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="562">
                <names>
                  <shortLabel>ecolx</shortLabel>
                  <fullName>Escherichia coli</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <intraMolecular>true</intraMolecular>
      </interaction>
    </interactionList>
  </entry>
</entrySet>