DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Galisteo ML., Yang Y., Ureña J., Schlessinger J. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Galisteo ML., Yang Y., Ureña J., Schlessinger J. DIP original experiment: DIP-263204X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Galisteo ML., Yang Y., Ureña J., Schlessinger J. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Galisteo ML., Yang Y., Ureña J., Schlessinger J. DIP original experiment: DIP-263203X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Galisteo ML., Yang Y., Ureña J., Schlessinger J. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Galisteo ML., Yang Y., Ureña J., Schlessinger J. DIP original experiment: DIP-263205X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Galisteo ML., Yang Y., Ureña J., Schlessinger J. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Galisteo ML., Yang Y., Ureña J., Schlessinger J. DIP original experiment: DIP-263207X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Galisteo ML., Yang Y., Ureña J., Schlessinger J. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Galisteo ML., Yang Y., Ureña J., Schlessinger J. DIP original experiment: DIP-263206X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli. nck1_mouse Cytoplasmic protein NCK1 Nck1 NCK adaptor protein 1 protein protein mouse Mus musculus Mouse MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDTASPADDSFVDPGERLYDLNMPAFVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQIGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTIMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS DIP protein Q99M51 original sequence version: 108 671BBFC870A88EB9 ack1_mouse Activated CDC42 kinase 1 Tnk2 Ack1 Non-receptor protein tyrosine kinase Ack Tyrosine kinase non-receptor protein 2 protein protein mouse Mus musculus Mouse MQPEEGTGWLLELLSEVQLQQYFLRLRDDLNITRLSHFEYVKNEDLEKIGMGRPGQRRLWEAVKRRKAMCKRKSWMSKVFSGKRLEAEFPSQHSQSTFRKPSPTPGSLPGEGTLQSLTCLIGEKDLRLLEKLGDGSFGVVRRGEWDAPAGKTVSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNLIRLYGVVLTLPMKMVTELAPLGSLLDRLRKHQGHFLLGTLSRYAVQVAEGMAYLESKRFIHRDLAARNLLLATRDLVKIGDFGLMRALPQNDDHYVMQEHRKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTYGQEPWIGLNGSQILHKIDKEGERLPRPEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTDMRALQDFEEPDKLHIQMNDVITVIEGRAENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGNPMDPPDLLSVELSTSRPTQHLGRVKREPPPRPPQPAIFTQKTTYDPVSEDPDPLSSDFKRLGLRKPALPRGLWLAKPSARVPGTKADRSSGGEVTLIDFGEEPVVPTPRPCAPSLAQLAMDACSLLDKTPPQSPTRALPRPLHPTPVVDWDARPLPPPPAYDDVAQDEDDFEVCSINSTLVGAGLPAGPSQGETNYAFVPEQAQMPPALEDNLFLPPQGGGKPPSSVQTAEIFQALQQECMRQLQVPTGQLTPSPTPGGDDKPQVPPRVPIPPRPTRPRVELSPAPSGEEETSRWPGPASPPRVPPREPLSPQGSRTPSPLVPPGSSPLPHRLSSSPGKTMPTTQSFASDPKYATPQVIQAPGPRAGPCILPIVRDGRKVSSTHYYLLPERPPYLERYQRFLREAQSPEEPAALPVPPLLPPPSTPAPAAPTATVRPMPQAAPDPKANFSTNNSNPGARPPSLRAAARLPQRGCPGDGQEAARPADKVQMLQAMVHGVTTEECQAALQSHSWSVQRAAQYLKVEQLFGLGLRPRVECHKVLEMFDWNLEQAGCHLLGSCGPAHHKR DIP protein O54967 original sequence version: 135 mint Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR. Participates in clathrin-mediated endocytosis. May be involved both in adult synaptic function and plasticity and in brain development 4A029C67C350B89A p16333-1 Cytoplasmic protein NCK1 NCK1 NCK NCK adaptor protein 1 SH2/SH3 adaptor protein NCK-alpha protein protein human Homo sapiens Human MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS DIP protein P16333-1 original sequence version: 165 DIP protein P16333 original sequence version: 163 DIP protein P16333 original sequence version: 160 nck1-tnk2-1 2 7 unspecified role unspecified role prey prey mouse Mus musculus Mouse 6 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association tnk2-tnk2-1 5 7 unspecified role unspecified role prey prey human Homo sapiens Human 7 unspecified role unspecified role bait bait Region 395-445 Region 395-445 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association tnk2-nck1-1 1 7 unspecified role unspecified role bait bait mouse Mus musculus Mouse 6 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association nck1-tnk2-2 3 7 unspecified role unspecified role prey prey human Homo sapiens Human 8 unspecified role unspecified role bait bait P16333-1:p.Arg308Ala R308A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R308A'. Wed May 02 15:23:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg308ala' Tue Jun 30 16:07:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg308Ala' human Homo sapiens Human physical association physical association nck1-tnk2-3 4 7 unspecified role unspecified role prey prey O54967:p.Lys158Ala K158A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain K A This feature has been corrected as a result of our quality control procedures. The original label was 'K158A'. Wed May 02 16:17:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys158ala' Tue Jun 30 20:13:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys158Ala' human Homo sapiens Human 8 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association