DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Lariguet P., Schepens I., Hodgson D., Pedmale UV., Trevisan M., Kami C., de Carbonnel M., Alonso JM., Ecker JR., Liscum E., Fankhauser C. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lariguet P., Schepens I., Hodgson D., Pedmale UV., Trevisan M., Kami C., de Carbonnel M., Alonso JM., Ecker JR., Liscum E., Fankhauser C. DIP original experiment: DIP-263237X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Lariguet P., Schepens I., Hodgson D., Pedmale UV., Trevisan M., Kami C., de Carbonnel M., Alonso JM., Ecker JR., Liscum E., Fankhauser C. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Lariguet P., Schepens I., Hodgson D., Pedmale UV., Trevisan M., Kami C., de Carbonnel M., Alonso JM., Ecker JR., Liscum E., Fankhauser C. DIP original experiment: DIP-263240X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Lariguet P., Schepens I., Hodgson D., Pedmale UV., Trevisan M., Kami C., de Carbonnel M., Alonso JM., Ecker JR., Liscum E., Fankhauser C. Only protein-protein interactions arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Lariguet P., Schepens I., Hodgson D., Pedmale UV., Trevisan M., Kami C., de Carbonnel M., Alonso JM., Ecker JR., Liscum E., Fankhauser C. DIP original experiment: DIP-263238X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Lariguet P., Schepens I., Hodgson D., Pedmale UV., Trevisan M., Kami C., de Carbonnel M., Alonso JM., Ecker JR., Liscum E., Fankhauser C. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Lariguet P., Schepens I., Hodgson D., Pedmale UV., Trevisan M., Kami C., de Carbonnel M., Alonso JM., Ecker JR., Liscum E., Fankhauser C. DIP original experiment: DIP-263239X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. pks1_arath Protein PHYTOCHROME KINASE SUBSTRATE 1 PKS1 At2g02950 T17M13.12 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MVTLTPSSASTPKTSFDFMKNNNSHSSLYVSSSSYLSSKEDALVTTKKLMEPSKTLNMSINPKQEEFGDEKKMVKKAPEDPEIGVFGAEKYFNGDMDSDQGSSVLSLTNPEVERTVVDSKQSAKKSTGTPSVRSESSWNSQSVLLQNKLVNSCNSSFKEKKNSNGQIQKVTNNKKSFLANLGCKCACSDGDSVDVEEKTSVKRSADPNISVITMRSSADMNTELIKIQKQEELSQRKSLEVFGSPVAIEKKSSVVQKKLPLPPWKSRTEEDDTKSEGSDSSSDLFEIEGLTGNPKPFLTRQGSDPASPTCYAPSEVSVEWSIVTASAADFSVMSECATSPVRRNRPTQIPRIPITAKSAPQRRKSSSSSGGNGFLMSCKSHKSVMVSGDLDRRSSMNKTQPSYVPRFPMETTKPKSFETRRRISNSSISHTQSSLLYSQ DIP protein Q9SWI1 original sequence version: 82 7A0A825FF7D3F3FC phot1_arath Phototropin-1 Non-phototropic hypocotyl protein 1 Root phototropism protein 1 PHOT1 JK224 NPH1 RPT1 T6D9_110 At3g45780 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEPTEKPSTKPSSRTLPRDTRGSLEVFNPSTQLTRPDNPVFRPEPPAWQNLSDPRGTSPQPRPQQEPAPSNPVRSDQEIAVTTSWMALKDPSPETISKKTITAEKPQKSAVAAEQRAAEWGLVLKTDTKTGKPQGVGVRNSGGTENDPNGKKTTSQRNSQNSCRSSGEMSDGDVPGGRSGIPRVSEDLKDALSTFQQTFVVSDATKPDYPIMYASAGFFNMTGYTSKEVVGRNCRFLQGSGTDADELAKIRETLAAGNNYCGRILNYKKDGTSFWNLLTIAPIKDESGKVLKFIGMQVEVSKHTEGAKEKALRPNGLPESLIRYDARQKDMATNSVTELVEAVKRPRALSESTNLHPFMTKSESDELPKKPARRMSENVVPSGRRNSGGGRRNSMQRINEIPEKKSRKSSLSFMGIKKKSESLDESIDDGFIEYGEEDDEISDRDERPESVDDKVRQKEMRKGIDLATTLERIEKNFVITDPRLPDNPIIFASDSFLELTEYSREEILGRNCRFLQGPETDLTTVKKIRNAIDNQTEVTVQLINYTKSGKKFWNIFHLQPMRDQKGEVQYFIGVQLDGSKHVEPVRNVIEETAVKEGEDLVKKTAVNIDEAVRELPDANMTPEDLWANHSKVVHCKPHRKDSPPWIAIQKVLESGEPIGLKHFKPVKPLGSGDTGSVHLVELVGTDQLFAMKAMDKAVMLNRNKVHRARAEREILDLLDHPFLPALYASFQTKTHICLITDYYPGGELFMLLDRQPRKVLKEDAVRFYAAQVVVALEYLHCQGIIYRDLKPENVLIQGNGDISLSDFDLSCLTSCKPQLLIPSIDEKKKKKQQKSQQTPIFMAEPMRASNSFVGTEEYIAPEIISGAGHTSAVDWWALGILMYEMLYGYTPFRGKTRQKTFTNVLQKDLKFPASIPASLQVKQLIFRLLQRDPKKRLGCFEGANEVKQHSFFKGINWALIRCTNPPELETPIFSGEAENGEKVVDPELEDLQTNVF DIP protein O48963 original sequence version: 137 C9B68812F1B3A04E rpt3_arath Root phototropism protein 3 RPT3 JK218 NPH3 BTB/POZ domain-containing protein RPT3 Non-phototropic hypocotyl protein 3 MSJ1.17 At5g64330 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MMWESESDGGVGVGGGGGREYGDGVLSSNKHGGVKTDGFELRGQSWFVATDIPSDLLVKIGDMNFHLHKYPLLSRSGKMNRLIYESRDPDPTILILDDLPGGPEAFELASKFCYGVPVDLTATNISGLRCAAEYLEMTEDLEEGNLIFKTEAFLSYVVLSSWRDSILVLKSCEKLSPWAENLQIVRRCSESIAWKACSNPKGIRWAYTGKAPSPSTTNFAGSSPRWNESKDSSFYCSPSRNTNSQPVPPDWWFEDVSILRIDHFVRVITAIKVKGMRFELLGAVIMHYAGKWLPGLIKEGGVAIAPAMSSAIGGGLGLGGDEMSISCGSNSSGGSSGPDWKGGLHMVLSAGKTNGHQDSVACLAGLGISPKDQRMIVESLISIIPPQKDSVTCSFLLRLLRAANMLKVAPALITELEKRVGMQFEQATLQDLLIPGYNNKGETMYDVDLVQRLLEHFLVQEQTEGSSPSRMSPSPSQSMYADIPRGNNNNGGGGGGNNQNAKMRVARLVDSYLTEVARDRNLPLTKFQVLAEALPESARTCDDGLYRAIDSYLKAHPTLSEHERKRLCRVMDCQKLSMDACMHAAQNERLPLRVVVQVLFSEQVKISNALANTSLKESTTLGEAMGTYQPMIPNRKTLIEATPQSFQEGWAAAKKDINTLKFELETVKTKYVELQNEMEVMQRQFEKTGKVKNTPSSSAWTSGWKKLSKLTKMSGQESHDISSGGEQAGVDHPPPRKPRRWRNSIS DIP protein Q9FMF5 original sequence version: 93 7D91D4224A5F3549 phot1-pks1-1 1 7 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 5 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 6 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress association association pks1-phot1-1 4 5 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction pks1-rpt3-1 2 7 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction pks1-phot1-2 3 7 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 5 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress 6 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress association association