DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. PLoS biology 2006 imex curation Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. DIP original experiment: DIP-66456X PLoS biology 2006 IMEx Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. PLoS biology 2006 imex curation Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. Only protein-protein interactions ecolx Escherichia coli nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. DIP original experiment: DIP-66455X PLoS biology 2006 IMEx Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. PLoS biology 2006 imex curation Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. DIP original experiment: DIP-66461X PLoS biology 2006 IMEx Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. PLoS biology 2006 imex curation Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. DIP original experiment: DIP-66459X PLoS biology 2006 IMEx Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. PLoS biology 2006 imex curation Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. Only protein-protein interactions ecolx Escherichia coli nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. DIP original experiment: DIP-66458X PLoS biology 2006 IMEx Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. PLoS biology 2006 imex curation Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. DIP original experiment: DIP-66462X PLoS biology 2006 IMEx Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. PLoS biology 2006 imex curation Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. DIP original experiment: DIP-66460X PLoS biology 2006 IMEx Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. PLoS biology 2006 imex curation Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lu J., Machius M., Dulubova I., Dai H., Südhof TC., Tomchick DR., Rizo J. DIP original experiment: DIP-66457X PLoS biology 2006 IMEx Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch. un13a_rat Protein unc-13 homolog A Unc13a Unc13h1 Munc13-1 protein protein rat Rattus norvegicus (Rat) Rat MSLLCVGVKKAKFDGAQEKFNTYVTLKVQNVKSTTIAVRGSQPSWEQDFMFEINRLDLGLTVEVWNKGLIWDTMVGTVWIPLRTIRQSNEEGPGEWLTLDSQAIMADSEICGTKDPTFHRILLDAHFELPLDIPEEEARYWAKKLEQLNAMRDQDEYSFQDQQDKPLPVPSSQCCNWNYFGWGEQNDDPDSAVDDRDSDYRSETSNSIPPPYYTTSQPNASVHQYSVRPPPLGSRESYSDSMHSYEEFSEPRALSPTGSSRYASSGELSQGSSQLSEDFDPDEHSLQGSELDDERDRDSYHSCHSSVSYHKDSPRWDQDEEDLEDLEDLEDEELPEEEELEEEELEEEEELEEEELELEEEEEVPDDLASYTQQEDTTVAEPKEFKRISFPTAAPQKEDKVSAVPIEAPDVSKGIPKAATPEEKAAAECAQEAEPPKSEESFRSREAEEGQEGQDAMSRAKANWLRAFNKVRMQLQEARGEGEMSKSLWFKGGPGGGLIIIDSMPDIRKRKPIPLVSDLAMSLVQSRKAGITSALASSTLNNEELKNHVYKKTLQALIYPISCTTPHNFEVWTATTPTYCYECEGLLWGIARQGMRCTECGVKCHEKCQDLLNADCLQRAAEKSSKHGAEDRTQNIIMVLKDRMKIRERNKPEIFELIQEVFAVTKSAHTQQMKAVKQSVLDGTSKWSAKISITVVCAQGLQAKDKTGSSDPYVTVQVGKTKKRTKTIYGNLNPVWEENFHFECHNSSDRIKVRVLDEDDDIKSRVKQRFKRESDDFLGQTIIEVRTLSGEMDVWYNLDKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHFVTDVQNNGVVKIPDAKGDDAWKVYYDETAQEIVDEFAMRYGVESIYQAMTHFACLSSKYMCPGVPAVMSTLLANINAYYAHTTASTNVSASDRFAASNFGKERFVKLLDQLHNSLRIDLSMYRNNFPASSPERLQDLKSTVDLLTSITFFRMKVQELQSPPRASQVVKDCVKACLNSTYEYIFNNCHELYGREYQTDPAKKGEVPPEEQGPSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWSLFAQDMKYAMEEHDKHRLCKSADYMNLHFKVKWLYNEYVAELPTFKDRVPEYPAWFEPFVIQWLDENEEVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGHYMRRFAKTISNVLLQYADIVSKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGGKELDAEASGTLKELQVKLNNVLDELSHVFATSFQPHIEECVRQMGDILSQVKGTGNVPASACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMERTIVLPPLTDQTMIGTLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAKSLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKTFVQTQSAQVHGGKGTRFTLSEDVCPEMGSGVEDPVGEVSVHVELFTHPGTGEQKVTVKVVAANDLKWQTSGIFRPFIEVNIVGPQLSDKKRKFATKSKNNSWAPKYNESFQFSLSADAGPECYELQVCVKDYCFAREDRTVELAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLKSDTRSAEEGGAAPAP DIP protein Q62768 original sequence version: 49 DIP protein Q62768-1 original sequence version: 90 rims2_rat Regulating synaptic membrane exocytosis protein 2 Rims2 Rim2 Rab-3-interacting molecule 2 protein protein rat Rattus norvegicus (Rat) Rat MSAPLGPRGRPAPTPAASQPPPQPEMPDLSHLTEEERKIIQAVMDRQKKEEEKEQSVLKKLHQQFEMYKEQVKKMGEESQQQQEQKGDAPTCGICHKTKFADGCGHNCSYCQTKFCARCGGRVSLRSNKVMWVCNLCRKQQEILTKSGAWFYNSGSNTPQQPDQKALRGLRSEEAPQEKKAKLHEQTQFQGPPGDSSVPAVERGRAHGLTRQDSIKNGSGMKHQIASDMPSDRKRSPSVSRDQNRRYDQSEEREEYSQYVPSDSTMPRSPSDYADRRSQREPQFYEEPDHLNYRDSNRRGHRHSKEYIVDDEDVESRDEYERQRREEEYQARYRSDPNLARYPVKPQPYEEQMRIHAEVSRARHERRHSDVSLANAELEDSRISLLRMDRPSRQRSVSERRAAMENQRSYSMERTREAQGQSSYPQRTTNHSPPTPRRSPIPLDRPELRRADSLRKQHHLDPSSAVRKTKREKMETMLRNDSLSSDQSESVRPPPPRPHKSKKGGKMRQVSLSSSEEELASTPEYTSCDDVEIESESVGEKGDMEYSWLEHASWHSSEASPMSLHPVTWQPSKDGDRLIGRILLNKRLKDGSVPRDSGAMLGLKVVGGKMTESGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLLQGATFEEVYNIILESKPEPQVELVVSRPIGDMPRIPDSTHAQLESSSSSFESQKMDRPSISVTSPMSPGMLRDVPQFLSGQLSSQSLSRRTTPFVPRVQIKLWFDKVGHQLIVTILGAKDLPSREDGRPRNPYVKIYFLPDRSDKNKRRTKTVKKTLEPKWNQTFIYSPVHRREFRERMLEITLWDQARVREEESEFLGEILIELETALLDDEPHWYKLQTHDVSSLPLPHPSPYMPRRQLHGESPTRRLQRSKRISDSEVSDYDCEDGVGVVSDYRHDGRDLQSSTLSVPEQVMSSNHCSPSGSPHRVDVIGRTRSWSPSVPPPQRNVEQGLRGTRATGHYNTISRMDRHRVMDDHYSSERDSHFLTLPRSRHRQTSEHHHRDGRDCEAADRQPYHRSRSTEQRPLLERTTTRSRSSERADTNLMRSMPSLMTGRSAPPSPALSRSHPRTGSVQTSPSSTPVTGRRGRQLPQLPPKGTLERMITEDMDSTRKRNSGAMDIEERNRQMKLNKYKQVAGSDPRLEQDYHSKYRSGWDPHRGADTVSTKSSDSDVSDVSAVSRTSSASRFSSTSYMSVQSERPRGNRKISVFTSKMQSRQMGVSGKSMAKSTSISGDMCSLEKNDGSQSDTAVGALGTSGKKRRSSIGAKMVAIVGLSRKSRSASQLSQTEGGGKKLRSTVQRSTETGLAVEMRNWMTRQASRESTDGSMNSYSSEGNLIFPGVRLASDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLDNGVCIAKKKTKVARKTLEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELELSNMVIGWFKLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYSRS DIP protein Q9JIS1 original sequence version: 46 DIP protein Q9JIS1 original sequence version: 1(def) mint Rab effector involved in exocytosis. May act as scaffold protein D76967BB36D9704E unc13a-1 1 9 unspecified role unspecified role unspecified role unspecified role C2A domain 3-150 C2A domain 3-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction unc13a-2 4 9 unspecified role unspecified role unspecified role unspecified role C2A domain 3-150 C2A domain 3-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rims2-unc13a-1 3 10 unspecified role unspecified role unspecified role unspecified role Region 82-142 Region 82-142 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role C2A domain 3-150 C2A domain 3-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction unc13a-3 8 9 unspecified role unspecified role unspecified role unspecified role C2A domain 3-150 C2A domain 3-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rims2-unc13a-2 7 10 unspecified role unspecified role unspecified role unspecified role Region 82-142 Region 82-142 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role C2A domain 3-150 C2A domain 3-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rims2-unc13a-3 6 10 unspecified role unspecified role unspecified role unspecified role Region 82-142 Region 82-142 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role C2A domain 3-150 C2A domain 3-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction unc13a-4 2 9 unspecified role unspecified role unspecified role unspecified role C2A domain 3-150 C2A domain 3-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15N-label 15N-label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction rims2-unc13a-4 5 10 unspecified role unspecified role unspecified role unspecified role Region 82-142 Region 82-142 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 9 unspecified role unspecified role unspecified role unspecified role C2A domain 3-150 C2A domain 3-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15N-label 15N-label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction