DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. Nature structural & molecular biology 2006 imex curation Shi H., Rojas R., Bonifacino JS., Hurley JH. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shi H., Rojas R., Bonifacino JS., Hurley JH. DIP original experiment: DIP-66231X Nature structural & molecular biology 2006 IMEx The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. Nature structural & molecular biology 2006 imex curation Shi H., Rojas R., Bonifacino JS., Hurley JH. Only protein-protein interactions human Homo sapiens Human coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip predetermined predetermined participant predetermined Shi H., Rojas R., Bonifacino JS., Hurley JH. DIP original experiment: DIP-66232X Nature structural & molecular biology 2006 IMEx The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. Nature structural & molecular biology 2006 imex curation Shi H., Rojas R., Bonifacino JS., Hurley JH. Only protein-protein interactions human Homo sapiens Human coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Shi H., Rojas R., Bonifacino JS., Hurley JH. DIP original experiment: DIP-66233X Nature structural & molecular biology 2006 IMEx The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. Nature structural & molecular biology 2006 imex curation Shi H., Rojas R., Bonifacino JS., Hurley JH. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Shi H., Rojas R., Bonifacino JS., Hurley JH. DIP original experiment: DIP-66234X Nature structural & molecular biology 2006 IMEx The retromer subunit Vps26 has an arrestin fold and binds Vps35 through its C-terminal domain. vp26a_human Vacuolar protein sorting-associated protein 26A VPS26A VPS26 Vesicle protein sorting 26A protein protein human Homo sapiens Human MSFLGGFFGPICEIDIVLNDGETRKMAEMKTEDGKVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRIEFVGQIELFNDKSNTHEFVNLVKELALPGELTQSRSYDFEFMQVEKPYESYIGANVRLRYFLKVTIVRRLTDLVKEYDLIVHQLATYPDVNNSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQLIKKEITGIGPSTTTETETIAKYEIMDGAPVKGESIPIRLFLAGYDPTPTMRDVNKKFSVRYFLNLVLVDEEDRRYFKQQEIILWRKAPEKLRKQRTNFHQRFESPESQASAEQPEM DIP protein O75436 original sequence version: 115 DIP protein O75436 original sequence version: 53 DIP protein O75436 original sequence version: 113 DIP protein O75436-1 original sequence version: 113 DIP protein O75436 original sequence version: 123 BD3B2EAA6CFCBFA9 pep8_yeast Carboxypeptidase Y-deficient protein 8 PEP8 GRD6 VPS26 VPT4 J1152 YJL053W Vacuolar protein-targeting protein 4 Vacuolar protein sorting-associated protein 26 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSIFFKPPIDIEILFDNEESRKHVDIATRSSNSSYKSMKESLPVYEDGESLGGIVTLRVRDSKKVDHLGIKVSVIGSIDMLKSHGSGNSSSKKVTSSTSSSSSNGSVDVRKNSVDQFLCQSYDLCPAGELQHSQSFPFLFRDLSKRYESYKGKNVDVAYYVKVTVMRKSTDISKIKRFWVYLYNSVTTAPNTLSANETKATTNDIAGGNYAADNASDNTQTKSTQGEAADVNQVLPISHSNNEPKPVRLDIGIENCLHIEFEYAKSQYSLKEVIVGRIYFLLTRLRIKHMELSLITRESSGLQTSNVMTDSTAIRYEIMDGSSVKGETIPIRLFLSGYDLTPNMSCNYFNVKNYLSLVIIDEDGRRYFKQSEITLYRTR 40D15594E70A9B58 40D15594E70A9B58 vps35_human Vacuolar protein sorting-associated protein 35 Vesicle protein sorting 35 Maternal-embryonic 3 VPS35 MEM3 TCCCTA00141 protein protein human Homo sapiens Human MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL DIP protein Q96QK1 original sequence version: 118 DIP protein Q96QK1 original sequence version: 44 DIP protein Q96QK1 original sequence version: 117 DIP protein Q96QK1 original sequence version: 127 28D2DD1C6B920A0A vps29_human Vacuolar protein sorting-associated protein 29 Vesicle protein sorting 29 PEP11 homolog VPS29 DC7 DC15 MDS007 protein protein human Homo sapiens Human MLVLVLGDLHIPHRCNSLPAKFKKLLVPGKIQHILCTGNLCTKESYDYLKTLAGDVHIVRGDFDENLNYPEQKVVTVGQFKIGLIHGHQVIPWGDMASLALLQRQFDVDILISGHTHKFEAFEHENKFYINPGSATGAYNALETNIIPSFVLMDIQASTVVTYVYQLIGDDVKVERIEYKKP DIP protein Q9UBQ0 original sequence version: 51 DIP protein Q9UBQ0-1 original sequence version: 117 DIP protein Q9UBQ0 original sequence version: 117 DIP protein Q9UBQ0 original sequence version: 129 6E0CDE6B720C9BF8 vps35-vps26a-8 1 5 unspecified role unspecified role prey prey 238-246 binding region 238-246 binding region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain O75436:p.[Ile235Asp;Met236Asp] I235D, M236D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M D certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I235D, M236D mutation'. Wed May 02 15:58:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile235asp,met236asp' Tue Jun 30 20:01:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile235Asp;Met236Asp]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association vps26a-vps35-4 2 7 unspecified role unspecified role prey prey human Homo sapiens Human 5 unspecified role unspecified role bait bait O75436:p.Gly238Pro G238P mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G P This feature has been corrected as a result of our quality control procedures. The original label was 'G238P mutation'. Wed May 02 20:01:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly238pro' Tue Jun 30 17:33:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly238Pro' 238-246 binding region 238-246 binding region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain MYC Tag MYC Tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range O75436:p.[Ile235Asp;Met236Asp] I235D, M236D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M D certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I235D, M236D mutation'. Wed May 02 16:26:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile235asp,met236asp' Tue Jun 30 16:03:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile235Asp;Met236Asp]' human Homo sapiens Human physical association physical association vps35-vps26p-1 4 6 unspecified role unspecified role prey prey 321-328 binding region 321-328 binding region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P40335:p.[Ile318Asp;Met319Asp] I318D, M319D mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M D certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I318D, M319D mutation'. Wed May 02 19:57:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile318asp,met319asp' Tue Jun 30 18:50:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile318Asp;Met319Asp]' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association vps26a-vps29-3 3 8 unspecified role unspecified role prey prey human Homo sapiens Human 5 unspecified role unspecified role bait bait 238-246 binding region 238-246 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain MYC Tag MYC Tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag full dna sequence full identification by DNA sequencing full dna sequence c-term range C-terminal range c-term range c-term range C-terminal range c-term range I235D, M236D mutation I235D, M236D mutation required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain O75436:p.Gly238Pro G238P mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G P This feature has been corrected as a result of our quality control procedures. The original label was 'G238P mutation'. Wed May 02 14:25:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly238pro' Tue Jun 30 18:57:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly238Pro' human Homo sapiens Human physical association physical association