DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. Only protein-protein interactions in vitro In vitro bn-page blue native page bn-page predetermined predetermined participant predetermined Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. DIP original experiment: DIP-262897X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. DIP original experiment: DIP-262895X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. DIP original experiment: DIP-262896X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. DIP original experiment: DIP-262894X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Paschos A., Patey G., Sivanesan D., Gao C., Bayliss R., Waksman G., O'callaghan D., Baron C. DIP original experiment: DIP-262898X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Dimerization and interactions of Brucella suis VirB8 with VirB4 and VirB10 are required for its biological activity. virba_brusu Type IV secretion system protein virB10 BS1330_II0060 virB10 BRA0060 protein protein brusu brusu MTQENIPVQPGTLDGERGLPTVNENGSGRTRKVLLFLFVVGFIVVLLLLLVFHMRGNAENNHHSDKTMVQTSTVPMRTFKLPPPPPPPPPAPPEPPAPPPAPAMPIAEPAAAALSLPPLPDDTPAKDDVLDKSASALMVVTKSSGDTNAQTAGDTVVQTTNARIQALLDSQKNTKQDAGSLGTLLHGTQTDARMASLLRNRDFLLAKGSIINCALQTRLDSTVPGMAACVVTRNMYSDNGKVLLIERGSTISGEYDANVKQGMARIYVLWTRVKTPNGVVIDLDSPGADPLGGAGLPGYIDSHFWKRFGGALMLSTIETLGRYATQKVGGGGSNQINLNTGGGESTSNLASTALKDTINIPPTLYKNQGEEIGIYIARDLDFSSVYDVKPK DIP protein Q9RPX5 original sequence version: 60 mint A7720A28B30194B6 virb4_brusu Type IV secretion system protein virB4 virB4 BRA0066 BS1330_II0066 protein protein brusu brusu MGAQSKYAQQLNNERSLAPFIPFRSQVGPTTVITRDGDFVRTWRIAGLAFETQDKEELLIRKDQLNTLFRAIASNNVALWSHNVRRRTWDHLKSFFSNPFCDALDKKYYGSFSGYRMMSNELYLTVIYRPVPAKISRLFNVAVHRSHAEILQEQQLAIRKLDEIGNQIETSLRRYGGDDGRGIEVLSTYEDKHGALCSQQLEFYNFLLSGEWQKVRVPSCPLDEYLGTGWVYAGTETIEIRTANATRYARGIDFKDYASHTEPGILNGLMYSDYEYVITQSFSFMTKRDGKEFLTRQKQRLQNTEDGSASQIMEMDIAIDQLGRGDFVMGEYHYSLLVFAEDMETVRHNTSHAMNILQDNGFLATVIATATDAAFYAQLPCNWRYRPRVAGLTSLNFAGLSCFHNFRAGKRDGNPWGQALTLLKTPSGQPAYLNFHYSKGDEDNFDKKLLGNTRIIGQSGAGKTVLMNFCLAQAQKYLHNAPMGMCNVFFDKDQGAKGTILAIGGKYLAIRNGEPTGFNPFQMEPTAGNILFLEKLVQVLVSRDGQHVTTTDESRISHAIRTVMRMRPELRRLSTVLQNVTEGSDRQDRENSVAKRLAKWCFDDGTGKRGTFWWVLDCPQDQIDFNTHSNYGFDGTDFLDNADVRTPISMYLLHRMELAIDGRRFIYWMDEAWKWVDDEAFSEFANNKQLTIRKQNGLGVFATQMPSSLLNSKVASALVQQVATEIYLPNPKADYHEYTDGFKVTNEEFDIIRSMSEESRMFLVKQGHHSMICRLELNGFDDELAILSGSSDNNELLDQVIAEVGDDPSVWLPVFQERRKARIASSKSTGR DIP protein Q9RPY1 original sequence version: 58 virb8_brusu Type IV secretion system protein virB8 BRA0062 BS1330_II0062 virB8 protein protein brusu brusu MFGRKQSPQKSVKNGQGNAPSVYDEALNWEAAHVRLVEKSERRAWKIAGAFGTITVLLGIGIAGMLPLKQHVPYLVRVNAQTGAPDILTSLDEKSVSYDTVMDKYWLSQYVIARETYDWYTLQKDYETVGMLSSPSEGQSYASQFQGDKALDKQYGSNVRTSVTIVSIVPNGKGIGTVRFAKTTKRTNETGDGETTHWIATIGYQYVNPSLMSESARLTNPLGFNVTSYRVDPEMGVVQ DIP protein Q7CEG3 original sequence version: 74 68F44CBCF7018023 virb8-3 4 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction virb4-virb8-1 1 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Q7CEG3:p.Arg230Asp R230D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R D This feature has been corrected as a result of our quality control procedures. The original label was 'R230D'. Wed May 02 14:28:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg230asp' Tue Jun 30 17:19:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg230Asp' ecolx Escherichia coli direct interaction direct interaction virb8-4 2 8 unspecified role unspecified role unspecified role unspecified role Q7CEG3:p.Met102Arg M102R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M R This feature has been corrected as a result of our quality control procedures. The original label was 'M102R'. Wed May 02 18:22:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met102arg' Tue Jun 30 16:18:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met102Arg' Q7CEG3:p.Tyr105Arg Y105R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y R This feature has been corrected as a result of our quality control procedures. The original label was 'Y105R'. Wed May 02 15:01:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr105arg' Tue Jun 30 16:45:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr105Arg' Q7CEG3:p.Glu214Arg E214R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E R This feature has been corrected as a result of our quality control procedures. The original label was 'E214R'. Wed May 02 14:53:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu214arg' Tue Jun 30 18:53:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu214Arg' ecolx Escherichia coli direct interaction direct interaction virb10-virb8-4 5 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role Q7CEG3:p.Thr201Tyr T201Y mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain T Y This feature has been corrected as a result of our quality control procedures. The original label was 'T201Y'. Wed May 02 17:10:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr201tyr' Tue Jun 30 17:57:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr201Tyr' Q7CEG3:p.Thr201Ala T201A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'T201A'. Wed May 02 18:03:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr201ala' Tue Jun 30 18:42:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr201Ala' ecolx Escherichia coli direct interaction direct interaction virb8-5 3 8 unspecified role unspecified role unspecified role unspecified role Q7CEG3:p.Met102Arg M102R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain M R This feature has been corrected as a result of our quality control procedures. The original label was 'M102R'. Wed May 02 18:13:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met102arg' Tue Jun 30 16:16:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met102Arg' Q7CEG3:p.Tyr105Arg Y105R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain Y R This feature has been corrected as a result of our quality control procedures. The original label was 'Y105R'. Wed May 02 19:49:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr105arg' Tue Jun 30 17:44:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr105Arg' Q7CEG3:p.Glu214Arg E214R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E R This feature has been corrected as a result of our quality control procedures. The original label was 'E214R'. Wed May 02 18:19:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu214arg' Tue Jun 30 19:28:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu214Arg' ecolx Escherichia coli direct interaction direct interaction