DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. DIP original experiment: DIP-262775X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. DIP original experiment: DIP-262774X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. Only protein-protein interactions in vitro In vitro deamination deamination assay aminase assay deamination predetermined predetermined participant predetermined Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. DIP original experiment: DIP-262777X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. Only protein-protein interactions in vitro In vitro deamination deamination assay aminase assay deamination predetermined predetermined participant predetermined Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. DIP original experiment: DIP-262779X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. DIP original experiment: DIP-262776X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. Only protein-protein interactions in vitro In vitro deamination deamination assay aminase assay deamination predetermined predetermined participant predetermined Arita K., Shimizu T., Hashimoto H., Hidaka Y., Yamada M., Sato M. DIP original experiment: DIP-262778X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4. h4(110) Histone 4 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) SGRGKGGKGL h3(1423) Histone 3 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KAPRKQLATK padi4_human Protein-arginine deiminase type-4 PADI4 PADI5 PDI5 Protein-arginine deiminase type IV Peptidylarginine deiminase IV HL-60 PAD PAD4 protein protein human Homo sapiens Human MAQGTLIRVTPEQPTHAVCVLGTLTQLDICSSAPEDCTSFSINASPGVVVDIAHGPPAKKKSTGSSTWPLDPGVEVTLTMKVASGSTGDQKVQISYYGPKTPPVKALLYLTGVEISLCADITRTGKVKPTRAVKDQRTWTWGPCGQGAILLVNCDRDNLESSAMDCEDDEVLDSEDLQDMSLMTLSTKTPKDFFTNHTLVLHVARSEMDKVRVFQATRGKLSSKCSVVLGPKWPSHYLMVPGGKHNMDFYVEALAFPDTDFPGLITLTISLLDTSNLELPEAVVFQDSVVFRVAPWIMTPNTQPPQEVYACSIFENEDFLKSVTTLAMKAKCKLTICPEEENMDDQWMQDEMEIGYIQAPHKTLPVVFDSPRNRGLKEFPIKRVMGPDFGYVTRGPQTGGISGLDSFGNLEVSPPVTVRGKEYPLGRILFGDSCYPSNDSRQMHQALQDFLSAQQVQAPVKLYSDWLSVGHVDEFLSFVPAPDRKGFRLLLASPRSCYKLFQEQQNEGHGEALLFEGIKKKKQQKIKNILSNKTLREHNSFVERCIDWNRELLKRELGLAESDIIDIPQLFKLKEFSKAEAFFPNMVNMLVLGKHLGIPKPFGPVINGRCCLEEKVCSLLEPLGLQCTFINDFFTYHIRHGEVHCGTNVRRKPFSFKWWNMVP DIP protein Q9UM07 original sequence version: 130 DIP protein Q9UM07 original sequence version: 127 79D7AF7B3D307A3B h3(413) Histone 3 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) KQTARKSTGG h3(413)-padi4-1 2 10 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction h3(413)-padi4-2 3 10 enzyme target enzyme target substrate substrate unspecified role unspecified role Arg5 Arg5 cit L-citrulline (S)-2-amino-5-(carbamoylamino)pentanoic acid 2-amino-5-(aminocarbonyl)aminopentanoic acid L-citrulline MOD_RES Citrulline N5-(aminocarbonyl)ornithine N5-carbamoylornithine N5-carbamylornithine alpha-amino-delta-ureidovaleric acid delta-ureidonorvaline Cit Citrulline Deamidated Deamidation citrullinationr certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro 9 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli deamination deamination reaction deamination h4(110)-padi4-1 4 7 enzyme target enzyme target substrate substrate unspecified role unspecified role Arg3 Arg3 cit L-citrulline (S)-2-amino-5-(carbamoylamino)pentanoic acid 2-amino-5-(aminocarbonyl)aminopentanoic acid L-citrulline MOD_RES Citrulline N5-(aminocarbonyl)ornithine N5-carbamoylornithine N5-carbamylornithine alpha-amino-delta-ureidovaleric acid delta-ureidonorvaline Cit Citrulline Deamidated Deamidation citrullinationr certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro 9 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli deamination deamination reaction deamination h3(1423)-padi4-1 1 8 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction h3(1423)-padi4-2 6 8 enzyme target enzyme target substrate substrate unspecified role unspecified role Arg4 Arg4 cit L-citrulline (S)-2-amino-5-(carbamoylamino)pentanoic acid 2-amino-5-(aminocarbonyl)aminopentanoic acid L-citrulline MOD_RES Citrulline N5-(aminocarbonyl)ornithine N5-carbamoylornithine N5-carbamylornithine alpha-amino-delta-ureidovaleric acid delta-ureidonorvaline Cit Citrulline Deamidated Deamidation citrullinationr certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm in vitro In vitro 9 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli deamination deamination reaction deamination h4(110)-padi4-2 5 7 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction