DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262733X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262734X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262738X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262725X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262726X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262735X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262728X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262727X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262730X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262737X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262740X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262741X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262732X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262731X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262739X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262729X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. Only protein-protein interactions in vitro In vitro epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Hanson SM., Francis DJ., Vishnivetskiy SA., Kolobova EA., Hubbell WL., Klug CS., Gurevich VV. DIP original experiment: DIP-262736X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Differential interaction of spin-labeled arrestin with inactive and active phosphorhodopsin. p08168-1 S-arrestin SAG 48 kDa protein Retinal S-antigen Rod photoreceptor arrestin protein protein bovin Bos taurus (Bovine) Bovine MKANKPAPNHVIFKKISRDKSVTIYLGKRDYIDHVERVEPVDGVVLVDPELVKGKRVYVSLTCAFRYGQEDIDVMGLSFRRDLYFSQVQVFPPVGASGATTRLQESLIKKLGANTYPFLLTFPDYLPCSVMLQPAPQDVGKSCGVDFEIKAFATHSTDVEEDKIPKKSSVRLLIRKVQHAPRDMGPQPRAEASWQFFMSDKPLRLAVSLSKEIYYHGEPIPVTVAVTNSTEKTVKKIKVLVEQVTNVVLYSSDYYIKTVAAEEAQEKVPPNSSLTKTLTLVPLLANNRERRGIALDGKIKHEDTNLASSTIIKEGIDKTVMGILVSYQIKVKLTVSGLLGELTSSEVATEVPFRLMHPQPEDPDTAKESFQDENFVFEEFARQNLKDAGEYKEEKTDQEAAMDE DIP protein P08168-1 original sequence version: 130 DIP protein P08168 original sequence version: 125 DIP protein P08168-1 original sequence version: 81 DIP protein P08168-1 original sequence version: 114 opsd_bovin Rhodopsin RHO protein protein bovin Bos taurus (Bovine) Bovine MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIMLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQVAPA DIP protein P02699 original sequence version: 157 DIP protein P02699 original sequence version: 158 DIP protein P02699 original sequence version: 88 DIP protein P02699 original sequence version: 160 DIP protein P02699 original sequence version: 152 DIP protein P02699 original sequence version: 136 DIP protein P02699 original sequence version: 135 DIP protein P02699 original sequence version: 141 mint Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal 33FDA196803E81F3 rho-sag-1 4 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role P08168-1:p.Leu77Cys L77C mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L C This feature has been corrected as a result of our quality control procedures. The original label was 'L77C'. Wed May 02 16:26:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu77cys' Tue Jun 30 19:41:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu77Cys' P08168-1:p.Phe79Cys F79C mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain F C This feature has been corrected as a result of our quality control procedures. The original label was 'F79C'. Wed May 02 14:50:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe79cys' Tue Jun 30 16:06:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe79Cys' Region 1-378 Region 1-378 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction rho-sag-2 15 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain Region 1-378 Region 1-378 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-3 2 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-4 9 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-5 11 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-6 5 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rho-sag-7 17 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-8 6 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-9 14 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-10 13 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-11 8 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-12 10 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-13 12 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-14 7 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-15 3 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-16 1 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rho-sag-17 16 19 unspecified role unspecified role unspecified role unspecified role bovin Bos taurus (Bovine) Bovine 18 unspecified role unspecified role unspecified role unspecified role R1-label R1-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction