DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit. CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hansen DV., Tung JJ., Jackson PK. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hansen DV., Tung JJ., Jackson PK. DIP original experiment: DIP-262497X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit. CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit. CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit. Proceedings of the National Academy of Sciences of the United States of America 2006 imex curation Hansen DV., Tung JJ., Jackson PK. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Hansen DV., Tung JJ., Jackson PK. DIP original experiment: DIP-262496X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx CaMKII and polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit. plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS DIP protein P53350 original sequence version: 169 DIP protein P53350 original sequence version: 170 DIP protein P53350 original sequence version: 164 DIP protein P53350 original sequence version: 154 DIP protein P53350 original sequence version: 91 DIP protein P53350 original sequence version: 145 DIP protein P53350 original sequence version: 121 DIP protein P53350 original sequence version: 118 DIP protein P53350 original sequence version: 123 DIP protein P53350 original sequence version: 119 DIP protein P53350 original sequence version: 138 DIP protein P53350 original sequence version: 163 DIP protein P53350 original sequence version: 178 178C2F13C10E8206 plk1_xenla Serine/threonine-protein kinase PLK1 plx1 Plx1 Polo-like kinase 1 plk1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MAQVAGKKLTVAPEAAKPPGIPGSSSAVKEIPEILVDPRTRRRYLRGRFLGKGGFAKCYEITDLESREVFAGKIVPKTMLLKPHQKDKMTMEIAIQRSLDHRHVVGFHGFFEDNDFVYVVLELCRRRSLLELHKRRKAVTEPEARYYLKQTISGCQYLHSNRVIHRDLKLGNLFLNDEMEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLGKKGHSFEVDIWSIGCIMYTLLVGKPPFETSCLKETYMRIKKNEYSIPKHINPVAAALIQKMLRSDPTSRPTIDDLLNDEFFTSGYIPSRLPTTCLTVPPRFSIAPSTIDQSLRKPLTAINKGQDSPLVEKQVAPAKEEEMQQPEFTEPADCYLSEMLQQLTCLNAVKPSERALIRQEEAEDPASIPIFWISKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLIMYNDGDSLQYIERNNTESYLNVRSYPTTLTKKITLLKYFRNYMSEHLLKAGANTTPREGDELARLPFLRTWFRTRSAIILHLSNGTVQINFFQDHTKIILCPLMAAVSYIDEKREFRTYKLSLIQEFGCCKELASRLRYARTMVEKLQSSKSAVAHVKASA DIP protein P70032 original sequence version: 107 DIP protein P70032 original sequence version: 61 2467195911F225E6 fbx43_xenla F-box only protein 43 fbxo43 emi2 erp1 fbx26 Early mitotic inhibitor 2 Emi1-related protein 1 F-box protein 26 Xerp1 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MANLLENFAAHHSMTAGAKKKADHQDTSVSQDSGYSDSLKGFSPDSHKSGNFLETVTEGYENSENIDPTLILSPIKYELSWGADTRESKQLAPLYETPRVGKKEFSLRRRLLISKATSGGNLDFDVSVCSAESCGREKSLRRIPSHEGSLSNSFADSPRDGSYEPIATSTLKTESESGTSCKKWRLSFAQQRSSTLDDSKSDSIPLPEVENISPVQHSLASSTDDSILYEETIFGAPTTPTCNFIVKEEFQTPISNLAANFRFNLCTPDVGHVSDFDISVTEDSAFHSLSLDKSQDSITDHEGSFQELIQKPRETSKAVNNKSRLRKLDRCRRLSTLRERGSQSEVEEEGNEVPVLSSAYKLKVARASVDEENEFSSDESRVHSLLSSDDLTGKPALRVLHEMLLRSTRKRPQQATVQDLLGSSGCFELPEDSLSRLIGRKMGLETFDILAELKNRNLKHILASILDLVNAASICSMCRVSRDWRDVVLQDKSAHQRRKAYIKKLKTEAEQGRQLSFEDSATRLNILSRSALRSVQIQARSAFRTPTSSLTPGDNKSIHSASKHQEYLKVAKTLFTDEALKPCPRCQYPAKYQALKKRGTCSRKDCGFDFCSLCLCTFHGSKECGTGSAKRIPKKEALPGSAQSKRNLKRL DIP protein Q8AXF4 original sequence version: 51 DIP protein Q8AXF4 original sequence version: 26 DIP protein Q8AXF4 original sequence version: 47 DIP protein Q8AXF4 original sequence version: 56 mint 48C53C789FE6491D fbxo43-plk1-1 2 5 unspecified role unspecified role bait bait Region 1-350 Region 1-350 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 4 unspecified role unspecified role prey prey xenla Xenopus laevis (African clawed frog) African clawed frog physical association physical association fbxo43-plk1-2 1 5 unspecified role unspecified role bait bait Region 1-350 Region 1-350 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 3 unspecified role unspecified role prey prey Region 326-603 Region 326-603 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction