DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Molecular architecture of a eukaryotic DNA transposase. Molecular architecture of a eukaryotic DNA transposase. Nature structural & molecular biology 2005 imex curation Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. Only protein-protein interactions ecolx Escherichia coli tem transmission electron microscopy tem predetermined predetermined participant predetermined Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. DIP original experiment: DIP-254785X Nature structural & molecular biology 2005 IMEx Molecular architecture of a eukaryotic DNA transposase. Molecular architecture of a eukaryotic DNA transposase. Molecular architecture of a eukaryotic DNA transposase. Nature structural & molecular biology 2005 imex curation Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. DIP original experiment: DIP-254784X Nature structural & molecular biology 2005 IMEx Molecular architecture of a eukaryotic DNA transposase. Molecular architecture of a eukaryotic DNA transposase. Molecular architecture of a eukaryotic DNA transposase. Nature structural & molecular biology 2005 imex curation Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. DIP original experiment: DIP-254786X Nature structural & molecular biology 2005 IMEx Molecular architecture of a eukaryotic DNA transposase. Molecular architecture of a eukaryotic DNA transposase. Molecular architecture of a eukaryotic DNA transposase. Nature structural & molecular biology 2005 imex curation Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hickman AB., Perez ZN., Zhou L., Musingarimi P., Ghirlando R., Hinshaw JE., Craig NL., Dyda F. DIP original experiment: DIP-254783X Nature structural & molecular biology 2005 IMEx Molecular architecture of a eukaryotic DNA transposase. q25442_musdo protein protein musdo Musca domestica House fly MQKMDNLEVKAKINQGLYKITPRHKGTSFIWNVLADIQKEDDTLVEGWVFCRKCEKVLKYTTRQTSNLCRHKCCASLKQSRELKTVSADCKKEAIEKCAQWVVRDCRPFSAVSGSGFIDMIKFFIKVGAEYGEHVNVEELLPSPITLSRKVTSDAKEKKALISREIKSAVEKDGASATIDLWTDNYIKRNFLGVTLHYHENNELRDLILGLKSLDFERSTAENIYKKLKAIFLQFNVEDLSSIKFVTDRGANVVKSLANNIRINCSSHLLSNVLENSFEETPELNVPILACKNIVKYFKKANLQHRLRSSLKSECPTRWNSTYTMLRSILDNWESVIQILSEAGETQRIVHINKSIIQTMVNILDGFERIFKELQTCSSPSLCFVVPSILKVKEICSPDVGDVADIAKLKVNIIKNVRIIWEENLSIWHYTAFFFYPPALHMQQEKVAQIKEFCLSKMEDLELINRMSSFNELSATQLNQSDSNSHNSIDLTSHSKDISTTSFFFPQLTQNNSREPPVCPSDEFEFYRKEIVILSEDFKVMEWWNLNSKKYPKLSKLALSLLSIPASSAASERTFSLAGNIITEKRNRIGQQTVDSLLFLNSFYKNFCKLDI DIP protein Q25442 original sequence version: 51 q25442_musdo-q25442_musdo-1 3 5 unspecified role unspecified role unspecified role unspecified role Region 79-162 Region 79-162 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 79-612 Region 79-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction q25442_musdo-1 4 5 unspecified role unspecified role unspecified role unspecified role Region 79-612 Region 79-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q25442:p.[Arg369Ala;Phe503Ala;Phe504Ala] R369A/F503A/F504A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R369A/F503A/F504A'. Wed May 02 17:16:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg369ala,phe503ala,phe504ala' Tue Jun 30 18:28:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg369Ala;Phe503Ala;Phe504Ala]' ecolx Escherichia coli direct interaction direct interaction q25442_musdo-2 2 5 unspecified role unspecified role unspecified role unspecified role Region 79-612 Region 79-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction q25442_musdo-3 1 5 unspecified role unspecified role unspecified role unspecified role Region 79-612 Region 79-612 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction