DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structure (London, England : 1993) 2005 imex curation Stirnimann CU., Rozhkova A., Grauschopf U., Grütter MG., Glockshuber R., Capitani G. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Stirnimann CU., Rozhkova A., Grauschopf U., Grütter MG., Glockshuber R., Capitani G. DIP original experiment: DIP-132807X Structure (London, England : 1993) 2005 IMEx Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structure (London, England : 1993) 2005 imex curation Stirnimann CU., Rozhkova A., Grauschopf U., Grütter MG., Glockshuber R., Capitani G. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Stirnimann CU., Rozhkova A., Grauschopf U., Grütter MG., Glockshuber R., Capitani G. DIP original experiment: DIP-132805X Structure (London, England : 1993) 2005 IMEx Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structure (London, England : 1993) 2005 imex curation Stirnimann CU., Rozhkova A., Grauschopf U., Grütter MG., Glockshuber R., Capitani G. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Stirnimann CU., Rozhkova A., Grauschopf U., Grütter MG., Glockshuber R., Capitani G. DIP original experiment: DIP-132806X Structure (London, England : 1993) 2005 IMEx Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structure (London, England : 1993) 2005 imex curation Stirnimann CU., Rozhkova A., Grauschopf U., Grütter MG., Glockshuber R., Capitani G. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Stirnimann CU., Rozhkova A., Grauschopf U., Grütter MG., Glockshuber R., Capitani G. DIP original experiment: DIP-132808X Structure (London, England : 1993) 2005 IMEx Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structural basis and kinetics of DsbD-dependent cytochrome c maturation. Structure (London, England : 1993) 2005 imex curation Stirnimann CU., Rozhkova A., Grauschopf U., Grütter MG., Glockshuber R., Capitani G. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Stirnimann CU., Rozhkova A., Grauschopf U., Grütter MG., Glockshuber R., Capitani G. DIP original experiment: DIP-132804X Structure (London, England : 1993) 2005 IMEx Structural basis and kinetics of DsbD-dependent cytochrome c maturation. dsba_ecoli Thiol:disulfide interchange protein DsbA dsbA dsf ppfA b3860 JW3832 protein protein ecoli Escherichia coli (strain K12) MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEFFSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALGVEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKAAADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK DIP protein P0AEG4 original sequence version: 53 DIP protein P0AEG4 original sequence version: 48 10527E876FCAEE55 dsbd_ecoli Thiol:disulfide interchange protein DsbD dsbD C-type cytochrome biogenesis protein CycZ Inner membrane copper tolerance protein Protein-disulfide reductase cutA2 cycZ dipZ JW5734 b4136 protein protein ecoli Escherichia coli (strain K12) MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQNQHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTLPVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAAPQPVSVPQQEQPTAQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMALTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLTLMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYALGMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSALGVAFFGWAFITSLQAKRGWMRIVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNFTQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQANVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP DIP protein P36655 original sequence version: 117 41EFAA1C9EAC6C5F dsbe_ecoli Thiol:disulfide interchange protein DsbE dsbE Cytochrome c biogenesis protein CcmG ccmG yejQ JW2183 b2195 protein protein ecoli Escherichia coli (strain K12) MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKPVPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVGMNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHAGDLNPRVWEEEIKPLWEKYSKEAAQ DIP protein P0AA86 original sequence version: 48 A599F7B77806A6B5 dsbd-dsbe-1 5 8 electron acceptor electron acceptor unspecified role unspecified role Region 43-185 Region 43-185 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 electron donor electron donor unspecified role unspecified role N-terminal domain Region 20-162 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli redox reaction oxidoreductase activity electron transfer reaction redox reaction dsbd-dsbe-2 3 8 unspecified role unspecified role unspecified role unspecified role Region 43-185 Region 43-185 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 20-162 Region 20-162 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction dsbe-dsbd-1 4 8 unspecified role unspecified role bait bait Region 43-185 Region 43-185 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey Region 20-162 Region 20-162 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction dsba-dsbe-1 2 6 electron acceptor electron acceptor unspecified role unspecified role ecolx Escherichia coli 8 electron donor electron donor unspecified role unspecified role Region 43-185 Region 43-185 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli redox reaction oxidoreductase activity electron transfer reaction redox reaction dsbd-dsbe-3 1 8 unspecified role unspecified role unspecified role unspecified role Region 43-185 Region 43-185 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 20-162 Region 20-162 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli covalent binding covalent binding