IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Molecular and cellular biology 2005 imex curation Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ., Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ. I have not included the DNA binding data as I have no idea how to represent a Holiday Junction in the editor. brill@mbcl.rutgers.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column western blot western blot Immuno blot Immuno blot Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ., Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ. I have not included the DNA binding data as I have no idea how to represent a Holiday Junction in the editor. brill@mbcl.rutgers.edu Accepted 2017-MAY-16 AT 12:47 BST AT 12:47 BST by MGT Molecular and cellular biology 2005 Only protein-protein interactions imex curation imex curation Its density gradient centrifugation we tend not to take - I've no problem with this. Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Molecular and cellular biology 2005 imex curation Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ., Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ. I have not included the DNA binding data as I have no idea how to represent a Holiday Junction in the editor. brill@mbcl.rutgers.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ., Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ. I have not included the DNA binding data as I have no idea how to represent a Holiday Junction in the editor. brill@mbcl.rutgers.edu Accepted 2017-MAY-16 AT 12:47 BST AT 12:47 BST by MGT Molecular and cellular biology 2005 Only protein-protein interactions imex curation imex curation Its density gradient centrifugation we tend not to take - I've no problem with this. Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Molecular and cellular biology 2005 imex curation Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ., Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ. I have not included the DNA binding data as I have no idea how to represent a Holiday Junction in the editor. brill@mbcl.rutgers.edu 8-Jun-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ., Mullen JR., Nallaseth FS., Lan YQ., Slagle CE., Brill SJ. I have not included the DNA binding data as I have no idea how to represent a Holiday Junction in the editor. brill@mbcl.rutgers.edu Accepted 2017-MAY-16 AT 12:47 BST AT 12:47 BST by MGT Molecular and cellular biology 2005 Only protein-protein interactions imex curation imex curation Its density gradient centrifugation we tend not to take - I've no problem with this. sgs1_yeast ATP-dependent helicase SGS1 SGS1 TPS1 YMR190C YM9646.02C Helicase TPS1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVTKPSHNLRREHKWLKETATLQEDKDFVFQAIQKHIANKRPKTNSPPTTPSKDECGPGTTNFITSIPASGPTNTATKQHEVMQTLSNDTEWLSYTATSNQYADVPMVDIPASTSVVSNPRTPNGSKTHNFNTFRPHMASSLVENDSSRNLGSRNNNKSVIDNSSIGKQLENDIKLEVIRLQGSLIMALKEQSKLLLQKCSIIESTSLSEDAKRLQLSRDIRPQLSNMSIRIDSLEKEIIKAKKDGMSKDQSKGRSQVSSQDDNIISSILPSPLEYNTSSRNSNLTSTTATTVTKALAITGAKQNITNNTGKNSNNDSNNDDLIQVLDDEDDIDCDPPVILKEGAPHSPAFPHLHMTSEEQDELTRRRNMRSREPVNYRIPDRDDPFDYVMGKSLRDDYPDVEREEDELTMEAEDDAHSSYMTTRDEEKEENELLNQSDFDFVVNDDLDPTQDTDYHDNMDVSANIQESSQEGDTRSTITLSQNKNVQVILSSPTAQSVPSNGQNQIGVEHIDLLEDDLEKDAILDDSMSFSFGRQHMPMSHSDLELIDSEKENEDFEEDNNNNGIEYLSDSDLERFDEERENRTQVADIQELDNDLKIITERKLTGDNEHPPPSWSPKIKREKSSVSQKDEEDDFDDDFSLSDIVSKSNLSSKTNGPTYPWSDEVLYRLHEVFKLPGFRPNQLEAVNATLQGKDVFVLMPTGGGKSLCYQLPAVVKSGKTHGTTIVISPLISLMQDQVEHLLNKNIKASMFSSRGTAEQRRQTFNLFINGLLDLVYISPEMISASEQCKRAISRLYADGKLARIVVDEAHCVSNWGHDFRPDYKELKFFKREYPDIPMIALTATASEQVRMDIIHNLELKEPVFLKQSFNRTNLYYEVNKKTKNTIFEICDAVKSRFKNQTGIIYCHSKKSCEQTSAQMQRNGIKCAYYHAGMEPDERLSVQKAWQADEIQVICATVAFGMGIDKPDVRFVYHFTVPRTLEGYYQETGRAGRDGNYSYCITYFSFRDIRTMQTMIQKDKNLDRENKEKHLNKLQQVMAYCDNVTDCRRKLVLSYFNEDFDSKLCHKNCDNCRNSANVINEERDVTEPAKKIVKLVESIQNERVTIIYCQDVFKGSRSSKIVQANHDTLEEHGIGKSMQKSEIERIFFHLITIRVLQEYSIMNNSGFASSYVKVGPNAKKLLTGKMEIKMQFTISAPNSRPSTSSSFQANEDNIPVIAQKSTTIGGNVAANPPRFISAKEHLRSYTYGGSTMGSSHPITLKNTSDLRSTQELNNLRMTYERLRELSLNLGNRMVPPVGNFMPDSILKKMAAILPMNDSAFATLGTVEDKYRRRFKYFKATIADLSKKRSSEDHEKYDTILNDEFVNRAAASSNGIAQSTGTKSKFFGANLNEAKENEQIINQIRQSQLPKNTTSSKSGTRSISKSSKKSANGRRGFRNYRGHYRGRK 0DC320B41756A3C3 0DC320B41756A3C3 top3_yeast DNA topoisomerase 3 TOP3 EDR1 YLR234W L8083.3 DNA topoisomerase III protein protein yeast Saccharomyces cerevisiae Baker's yeast MKVLCVAEKNSIAKAVSQILGGGRSTSRDSGYMYVKNYDFMFSGFPFARNGANCEVTMTSVAGHLTGIDFSHDSHGWGKCAIQELFDAPLNEIMNNNQKKIASNIKREARNADYLMIWTDCDREGEYIGWEIWQEAKRGNRLIQNDQVYRAVFSHLERQHILNAARNPSRLDMKSVHAVGTRIEIDLRAGVTFTRLLTETLRNKLRNQATMTKDGAKHRGGNKNDSQVVSYGTCQFPTLGFVVDRFERIRNFVPEEFWYIQLVVENKDNGGTTTFQWDRGHLFDRLSVLTFYETCIETAGNVAQVVDLKSKPTTKYRPLPLTTVELQKNCARYLRLNAKQSLDAAEKLYQKGFISYPRTETDTFPHAMDLKSLVEKQAQLDQLAAGGRTAWASYAASLLQPENTSNNNKFKFPRSGSHDDKAHPPIHPIVSLGPEANVSPVERRVYEYVARHFLACCSEDAKGQSMTLVLDWAVERFSASGLVVLERNFLDVYPWARWETTKQLPRLEMNALVDIAKAEMKAGTTAPPKPMTESELILLMDTNGIGTDATIAEHIDKIQVRNYVRSEKVGKETYLQPTTLGVSLVHGFEAIGLEDSFAKPFQRREMEQDLKKICEGHASKTDVVKDIVEKYRKYWHKTNACKNTLLQVYDRVKASM 51DF78936A88B4F4 51DF78936A88B4F4 rmi1_yeast RecQ-mediated genome instability protein 1 RMI1 NCE4 YPL024W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSFSSILSQDITDDITPPAYSATLGSREQIVFRAYQNEPWLAGTASNLILDKKLVIVDRELLFQVLMVENITKSKLTQIDDIKTKLDPKKQKVDRLRSGAQGNGAKKYEVITQVDMEDDGNVADNNCAKENNSNNNSSAAKNKAVFKLTLQSKSGDVFFAINSTPISWSSCMLGSKIVILPGTVFNRGVFILKDSQVIFLGGINRVWNENRDQKFCDYLESKLQRDKQLVNGGSKKRKAND 65EF152F50BF0084 65EF152F50BF0084 rmi1-sgs1-1 3 6 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig. 4A sgs1-rmi1-2 3 6 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig. 4A top3-rmi1-1 3 6 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role bait bait c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range association association Fig. 4A top3-rmi1-2 3 6 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role bait bait c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4A Interaction observed in SGS1-/- rmi1-top3-1 3 6 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4A Interaction observed in SGS1-/- rmi1-sgs1-2 1 6 unspecified role unspecified role neutral component neutral component c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role neutral component neutral component c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4B rmi1-top3-2 2 6 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 5B rmi1-sgs1-3 2 6 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 5D, 5F top3-rmi1-3 2 5 unspecified role unspecified role bait bait c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 5A sgs1-rmi1-3 2 6 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait c-terminal v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 5 C/E