UniProt UniProt http://www.uniprot.org Posttranscriptional regulation of HO expression by the Mkt1-Pbp1 complex. Posttranscriptional regulation of HO expression by the Mkt1-Pbp1 complex. Molecular and Cellular Biology 2004 imex curation Tadauchi T., Inada T., Matsumoto K., Irie K. g44177a@nucc.cc.nagoya-u.ac.jp 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tadauchi T., Inada T., Matsumoto K., Irie K. g44177a@nucc.cc.nagoya-u.ac.jp Accepted 2017-SEP-27 AT 08:58 BST AT 08:58 BST by MGT Molecular and Cellular Biology 2004 imex curation Posttranscriptional regulation of HO expression by the Mkt1-Pbp1 complex. Posttranscriptional regulation of HO expression by the Mkt1-Pbp1 complex. Molecular and Cellular Biology 2004 imex curation Tadauchi T., Inada T., Matsumoto K., Irie K. g44177a@nucc.cc.nagoya-u.ac.jp 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tadauchi T., Inada T., Matsumoto K., Irie K. g44177a@nucc.cc.nagoya-u.ac.jp Accepted 2017-SEP-27 AT 08:58 BST AT 08:58 BST by MGT Molecular and Cellular Biology 2004 imex curation Posttranscriptional regulation of HO expression by the Mkt1-Pbp1 complex. Posttranscriptional regulation of HO expression by the Mkt1-Pbp1 complex. Molecular and Cellular Biology 2004 imex curation Tadauchi T., Inada T., Matsumoto K., Irie K. g44177a@nucc.cc.nagoya-u.ac.jp 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tadauchi T., Inada T., Matsumoto K., Irie K. g44177a@nucc.cc.nagoya-u.ac.jp Accepted 2017-SEP-27 AT 08:58 BST AT 08:58 BST by MGT Molecular and Cellular Biology 2004 imex curation pbp1_yeast PAB1-binding protein 1 PBP1 MRS16 Poly(A)-binding protein-binding protein YGR178C protein protein yeast Saccharomyces cerevisiae Baker's yeast MKGNFRKRDSSTNSRKGGNSDSNYTNGGVPNQNNSSMFYENPEITRNFDDRQDYLLANSIGSDVTVTVTSGVKYTGLLVSCNLESTNGIDVVLRFPRVADSGVSDSVDDLAKTLGETLLIHGEDVAELELKNIDLSLDEKWENSKAQETTPARTNIEKERVNGESNEVTKFRTDVDISGSGREIKERKLEKWTPEEGAEHFDINKGKALEDDSASWDQFAVNEKKFGVKSTFDEHLYTTKINKDDPNYSKRLQEAERIAKEIESQGTSGNIHIAEDRGIIIDDSGLDEEDLYSGVDRRGDELLAALKSNSKPNSNKGNRYVPPTLRQQPHHMDPAIISSSNSNKNENAVSTDTSTPAAAGAPEGKPPQKTSKNKKSLSSKEAQIEELKKFSEKFKVPYDIPKDMLEVLKRSSSTLKSNSSLPPKPISKTPSAKTVSPTTQISAGKSESRRSGSNISQGQSSTGHTTRSSTSLRRRNHGSFFGAKNPHTNDAKRVLFGKSFNMFIKSKEAHDEKKKGDDASENMEPFFIEKPYFTAPTWLNTIEESYKTFFPDEDTAIQEAQTRFQQRQLNSMGNAVPGMNPAMGMNMGGMMGFPMGGPSASPNPMMNGFAAGSMGMYMPFQPQPMFYHPSMPQMMPVMGSNGAEEGGGNISPHVPAGFMAAGPGAPMGAFGYPGGIPFQGMMGSGPSGMPANGSAMHSHGHSRNYHQTSHHGHHNSSTSGHK 92005F3A2346193E 92005F3A2346193E mkt1_yeast Post-transcriptional regulator MKT1 MKT1 Inactive endonuclease MKT1 N2302 YNL085W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAIKSLESFLFERGLVGSYAIEALNNCTLDIDVNHYVSRLLTNKREQYLDAIGGFPTSLKMYLESDLKIFKDFNITPIFVFNGGLTYNQLEASGHFTAASASASISSTTTSSSGTNATTRSNTESVLLQRSRGWTQWNNLISSNQNSYIDQPIQPQEPFRHNTTIDSKAYQNDLIAYFIEHGYMYQVAPYSSWFQLAYLLNSAYIDAIYGPTDCLMLDCVDRFILGMEFPNKEFRFIDRSRVMKDLGCTHEEFIDIAMAVGNDLQPTTLPPLQIYPVPQLFDIALEMVLNTGTNFYAYQLSTTLQNDSKENIQNYQRGISALRYMPVLKDTGKVELFVQEIVVSEEDSEKNNKDGKKSNLSSPSSASSSASPATTVTKNASEKLTYEKSSTKEVRKPRDIPNDVHDFIGQMLPHEYYFYRSIGLVTGKLFDAIVTGVYPEEPPLGGGSSTSYKKLVSKSVEIFKNKEINLLTQPINRYYQIKQIKQVKWYAANEPTTLTNRMSPSMFETINHLIVKTETSDEKEFSISEFITTINGSSNMAKDFISEKVIFPNSVPIESKLNSPFNLLSTNFLRLLVLLEFFTFDFKEKLLEPTRWGEVFLKLNELNIDSKYHESVIIFLVFLKCDVLKLDEEVQPPAPSALSQATLRSYPEESLYVLLITRVLTLFQVDQKPSNYHGPIDKKTLIFRDHLSFIKENLNELFEAVLISSLTSGEFNRLSLDNFGWARKIVRYLPFKLDSPNTIMAMMWEFFLQKYLHNGNAKNDALSLVATEFNTYKSTPNLDEQFVESHRFLLEISKVMQELNAAKLIDENVFKLFTKAVEFTTTALSS BD976D8387E4F708 BD976D8387E4F708 mkt1-pbp1-1 3 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait physical association physical association mkt1-pbp1-2 2 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 14 12 physical association physical association Fig. 5A Two-hybrid analysis revealed that the COOH-terminal region of Mkt1 is required for its interaction with Pbp1 ​(Fig.5A). mkt1-pbp1-3 1 4 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 5B To confirm that Mkt1 binds Pbp1 in vivo, we performed immunoprecipitation analyses.