DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262978X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262973X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262976X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262977X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262966X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262965X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast pull down pull down western blot western blot Immuno blot Immuno blot Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262967X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262974X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262964X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262969X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262968X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262975X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262972X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262970X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. UBA domains of DNA damage-inducible proteins interact with ubiquitin. Nature structural biology 2001 imex curation Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. Only protein-protein interactions in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Bertolaet BL., Clarke DJ., Wolff M., Watson MH., Henze M., Divita G., Reed SI. DIP original experiment: DIP-262971X Nature structural biology 2001 IMEx UBA domains of DNA damage-inducible proteins interact with ubiquitin. ddi1_yeast DNA damage-inducible protein 1 DDI1 v-SNARE-master 1 VSM1 YER143W protein protein yeast Saccharomyces cerevisiae Baker's yeast MDLTISNELTGEIYGPIEVSEDMALTDLIALLQADCGFDKTKHDLYYNMDILDSNRTQSLKELGLKTDDLLLIRGKISNSIQTDAATLSDEAFIEQFRQELLNNQMLRSQLILQIPGLNDLVNDPLLFRERLGPLILQRRYGGYNTAMNPFGIPQDEYTRLMANPDDPDNKKRIAELLDQQAIDEQLRNAIEYTPEMFTQVPMLYINIEINNYPVKAFVDTGAQTTIMSTRLAKKTGLSRMIDKRFIGEARGVGTGKIIGRIHQAQVKIETQYIPCSFTVLDTDIDVLIGLDMLKRHLACVDLKENVLRIAEVETSFLSEAEIPKSFQEGLPAPTSVTTSSDKPLTPTKTSSTLPPQPGAVPALAPRTGMGPTPTGRSTAGATTATGRTFPEQTIKQLMDLGFPRDAVVKALKQTNGNAEFAASLLFQ B43425B029B7658B B43425B029B7658B ubiq_yeast Ubiquitin UBI1 RPL40A YIL148W UBI2 RPL40B YKR094C UBI3 RPS31 YLR167W L9470.14 UBI4 SCD2 YLL039C protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG obsolete protein 9AC936269D38403E rad23_yeast UV excision repair protein RAD23 SYGP-ORF29 YEL037C RAD23 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSLTFKNFKKEKVPLDLEPSNTILETKTKLAQSISCEESQIKLIYSGKVLQDSKTVSECGLKDGDQVVFMVSQKKSTKTKVTEPPIAPESATTPGRENSTEASPSTDASAAPAATAPEGSQPQEEQTATTERTESASTPGFVVGTERNETIERIMEMGYQREEVERALRAAFNNPDRAVEYLLMGIPENLRQPEPQQQTAAAAEQPSTAATTAEQPAEDDLFAQAAQGGNASSGALGTTGGATDAAQGGPPGSIGLTVEDLLSLRQVVSGNPEALAPLLENISARYPQLREHIMANPEVFVSMLLEAVGDNMQDVMEGADDMVEGEDIEVTGEAAAAGLGQGEGEGSFQVDYTPEDDQAISRLCELGFERDLVIQVYFACDKNEEAAANILFSDHAD B3F0436DAB60B833 B3F0436DAB60B833 ub-ddi1-1 9 17 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role prey prey UBA UBA Domain/necessary required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rad23-ub-1 10 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction ub-ddi1-2 8 17 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role prey prey UBA UBA Domain/sufficient sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ub-ddi1-3 1 17 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role prey prey P40087:p.Lys413Ile K413I Mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain K I This feature has been corrected as a result of our quality control procedures. The original label was 'K413I Mutation'. Wed May 02 17:38:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys413ile' Tue Jun 30 15:53:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys413Ile' P40087:p.Gly417Arg G417R Mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G R This feature has been corrected as a result of our quality control procedures. The original label was 'G417R Mutation'. Wed May 02 15:47:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly417arg' Tue Jun 30 16:33:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly417Arg' P40087:p.Gly417Val G417V Mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain G V This feature has been corrected as a result of our quality control procedures. The original label was 'G417V Mutation'. Wed May 02 16:48:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly417val' Tue Jun 30 20:13:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly417Val' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ub-rad23-1 6 18 unspecified role unspecified role prey prey 78-354 binding region 78-354 binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 17 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ub-ddi1-4 12 17 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role prey prey P40087:p.Leu426Ala L426A Mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L426A Mutation'. Wed May 02 17:13:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu426ala' Tue Jun 30 18:00:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu426Ala' P40087:p.Val408Leu V408L Mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain V L This feature has been corrected as a result of our quality control procedures. The original label was 'V408L Mutation'. Wed May 02 16:33:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val408leu' Tue Jun 30 20:05:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val408Leu' P40087:p.Ala407Leu V403E Mutation mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain A L Wed May 02 15:59:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala407leu' Tue Jun 30 18:00:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala407Leu' P40087:p.Leu412Pro L412P Mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L P This feature has been corrected as a result of our quality control procedures. The original label was 'L412P Mutation'. Wed May 02 14:38:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu412pro' Tue Jun 30 16:09:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu412Pro' P40087:p.Leu426His L426H Mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L H This feature has been corrected as a result of our quality control procedures. The original label was 'L426H Mutation'. Wed May 02 17:41:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu426his' Tue Jun 30 17:07:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu426His' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ddi1-ub-1 14 17 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role bait bait His Tag His Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association ub-ddi1-5 5 17 unspecified role unspecified role bait bait GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ub-rad23-2 3 18 unspecified role unspecified role prey prey P32628:p.Leu183Ala L183A Mutation mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L183A Mutation'. Wed May 02 17:05:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu183ala' Tue Jun 30 15:41:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu183Ala' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 17 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rad23-ub-2 15 18 unspecified role unspecified role bait bait His Tag His Tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 17 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction ddi1-ub-2 13 17 unspecified role unspecified role unspecified role unspecified role Dansyl Label Dansyl Label dansyl label dansyl label 5-dimethylaminonaphthalene-1-sulfonyl tag dansyl tag 5-dimethylaminonaphthalene-1-sulfonyl tag predetermined featur predetermined feature predetermined featur c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ub-rad23-3 7 18 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 17 unspecified role unspecified role bait bait GST Tag GST Tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ub-rad23-4 4 18 unspecified role unspecified role prey prey P32628:p.Leu392Ala L392A Mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L392A Mutation'. Wed May 02 16:45:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu392ala' Tue Jun 30 19:11:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu392Ala' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 17 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ddi1-ub-3 11 17 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction rad23-ub-3 2 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role Dansyl Label Dansyl Label dansyl label dansyl label 5-dimethylaminonaphthalene-1-sulfonyl tag dansyl tag 5-dimethylaminonaphthalene-1-sulfonyl tag predetermined featur predetermined feature predetermined featur c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction