DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. The Journal of biological chemistry 2001 imex curation Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. Only protein-protein interactions in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. DIP original experiment: DIP-262176X The Journal of biological chemistry 2001 IMEx Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. The Journal of biological chemistry 2001 imex curation Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. DIP original experiment: DIP-262177X The Journal of biological chemistry 2001 IMEx Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. The Journal of biological chemistry 2001 imex curation Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. DIP original experiment: DIP-262178X The Journal of biological chemistry 2001 IMEx Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. The Journal of biological chemistry 2001 imex curation Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Silversmith RE., Smith JG., Guanga GP., Les JT., Bourret RB. DIP original experiment: DIP-262179X The Journal of biological chemistry 2001 IMEx Alteration of a nonconserved active site residue in the chemotaxis response regulator CheY affects phosphorylation and interaction with CheZ. chey_ecoli Chemotaxis protein CheY cheY b1882 JW1871 protein protein ecoli Escherichia coli (strain K12) MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM DIP protein P0AE67 original sequence version: 95 DIP protein P0AE67 original sequence version: 56 DIP protein P0AE67 original sequence version: 96 E4B60B8A73DA14DC chez_ecoli Protein phosphatase CheZ cheZ b1881 JW1870 Chemotaxis protein CheZ protein protein ecoli Escherichia coli (strain K12) MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEAAEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPIDLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIERQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF DIP protein P0A9H9 original sequence version: 75 42308F5AC0685D27 chey-chez-3 4 6 unspecified role unspecified role unspecified role unspecified role F214C fluorescein label F214C fluorescein label fluor-5-maleimide fluorescein-5-maleimide label fluor-5-maleimide predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction chey-chez-5 1 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction chez-1 2 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction chey-chez-6 3 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction