UniProt UniProt http://www.uniprot.org The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity. The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity. RNA (New York, N.Y.) 2000 imex curation Rho SB., Martinis SA. smartinis@uh.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 3 hybrid three hybrid 3-hybrid tri hybrid tri-hybrid assay 3 hybrid predetermined predetermined participant predetermined Rho SB., Martinis SA. The DNA binding domain -LexADB was fused to an RNA binding protein -MS2-coat protein rather than Hiv-1 RevM10. A third component is hybrid RNA comprised of MS2 RNA fused to the RNA of interest (Bait). The protein prey fused to B42-AD. smartinis@uh.edu Accepted 2017-OCT-09 AT 10:08 BST AT 10:08 BST by MGT RNA (New York, N.Y.) 2000 imex curation The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity. The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity. RNA (New York, N.Y.) 2000 imex curation Rho SB., Martinis SA. smartinis@uh.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rho SB., Martinis SA. smartinis@uh.edu Accepted 2017-OCT-09 AT 10:08 BST AT 10:08 BST by MGT RNA (New York, N.Y.) 2000 imex curation The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity. The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity. RNA (New York, N.Y.) 2000 imex curation Rho SB., Martinis SA. smartinis@uh.edu 15-Nov-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeast Saccharomyces cerevisiae Baker's yeast rna three hybrid rna three hybrid Three hybrid system rna 3-hybrid rna tri hybrid rna-three hybrid predetermined predetermined participant predetermined Rho SB., Martinis SA. The DNA binding domain -LexADB was fused to an RNA binding protein -MS2-coat protein rather than Hiv-1 RevM10. A third component is hybrid RNA comprised of MS2 RNA fused to the RNA of interest (Bait). The protein prey fused to B42-AD. smartinis@uh.edu Accepted 2017-OCT-09 AT 10:08 BST AT 10:08 BST by MGT RNA (New York, N.Y.) 2000 imex curation bi4_intron_yeast_mrna bI4 intron RNA mrna messenger rna mRNA mrna yeast Saccharomyces cerevisiae Baker's yeast UCAAAAUAUGGCCUUAUUAUUAAUUACAUAUGUAAUUAAUAUUUUAUGUGCUGUAUGCUGGAAAUCUUUAUUUAUUAAAUAUCAAUGAAAAAUUUAUAAUAAAACUCUAUAUUAUUUUAUUAUUCAAAAUAUUUUAAAUACAAAACAAUUAAAUAAUUUCGUAUUAAAAUUUAAUUGAACAAAGCAAUAUAAUAAAAUAAAUAUUGUAAGUGAUUUAUUUAAUCCCAAUAGAGUAAAAUAUUAUUAUAAAGAAGAUAAUCAGCAGGUAACCAAUAUAAAUUCUUCUAAUACUCACUUAACGAGUAAUAAAAAGAAUUUAUUAGUAGAUACUUCAGAGACUACACGCACACUAAAAAAUAAAUUUAAUUAUUUAUUAAAUAUUUUUAAUAUAAAAAAAAUAAAUCAAAUUAUUCUUAAAAGACAUUAUAGUAUUUAUAAAGAUAGUAAUAUUAGAUUUAACCAAUGAUUGGCCGGUUUAAUUGACGGAGAUGGUUAUUUUUGUAUUACUAAAAAUAAAUAUGCAUCUUGUGAAAUUCUUGUAGAAUUAAAAGAUGAAAAAAUGUUAAGACAAAUCCAAGAUAAAUUUGGUGGUUCUGUAAAAUUAAGAUCAGGUGUUAAGGCUAUUAGAUAUAGAUUACAAAAUAAAGAAGGUAUAAUUAAAUUAAUUAAUGCCGUUAAUGGUAAUAUUCGUAAUAGUAAAAGAUUAGUACAAUUUAAUAAAGUAUGUAUUUUAUUAAAUAUCGAUUUUAAAGAACCUAUUAAAUUAACUAAAGAUAAUGCUUGAUUUAUAGGGUUCUUUGAUGCUGAUGGUACUAUUAAUUAUUAUUAUUCCGGUAAAUUAAAAAUUAGACCUCAAUUAACUAUUAGCGUUACAAAUAAAUAUUUACAUGAUGUUGAAUACUAUAGAGAAGUAUUUGGUGGUAAUAUUUAUUUUGAUAAAGCUAAAAAUGGUUAUUUUAAAUGAUCUAUUAAUAAUAAAGAAUUACAUAAUAUUUUUUAUCUUUAUAAUAAAAGUUGUCCUUCUAAAUCUAAUAAAGGUAAACGUUUAUUUUUAAUUGAUAAAUUUUAUUAUUUAUAUGAUUUAUUAGCUUUUAAAGCACCUCAUAAUACUGCUUUAUAUAAAGCUUGAUUAAAAUUUAAUGAAAAAUGAAAUAAUAAUUAAAUUUUCUCCGUAUUCAUUAUUAUAUUAUCUAAUUUAUAAAAUAUUUAAAGAUUCCUUAUAAUAAUAUAACAUCUUUGUAAAUUAUUGUUAAAGAUAAUAUAAAUUAUUAUGAAUCGGUAGAUUAUAUUUUUACAAUCUUAUUAAAUAAAAUUCUGAUCAUUAAACAUGAUUGAAGAAAUAAUAAUAGUUUAUGAAAUAAGAUAGUGUAAUAUAAAUUUUUAUGAAGAUAUAGUCCAUUUUAUAUUUAUUAUAAAAG syic_human Isoleucine--tRNA ligase, cytoplasmic IARS1 IARS Isoleucyl-tRNA synthetase protein protein human Homo sapiens Human MLQQVPENINFPAEEEKILEFWTEFNCFQECLKQSKHKPKFTFYDGPPFATGLPHYGHILAGTIKDIVTRYAHQSGFHVDRRFGWDCHGLPVEYEIDKTLGIRGPEDVAKMGITEYNNQCRAIVMRYSAEWKSTVSRLGRWIDFDNDYKTLYPQFMESVWWVFKQLYDKGLVYRGVKVMPFSTACNTPLSNFESHQNYKDVQDPSVFVTFPLEEDETVSLVAWTTTPWTLPSNLAVCVNPEMQYVKIKDVARGRLLILMEARLSALYKLESDYEILERFPGAYLKGKKYRPLFDYFLKCKENGAFTVLVDNYVKEEEGTGVVHQAPYFGAEDYRVCMDFNIIRKDSLPVCPVDASGCFTTEVTDFAGQYVKDADKSIIRTLKEQGRLLVATTFTHSYPFCWRSDTPLIYKAVPSWFVRVENMVDQLLRNNDLCYWVPELVREKRFGNWLKDARDWTISRNRYWGTPIPLWVSDDFEEVVCIGSVAELEELSGAKISDLHRESVDHLTIPSRCGKGSLHRISEVFDCWFESGSMPYAQVHYPFENKREFEDAFPADFIAEGIDQTRGWFYTLLVLATALFGQPPFKNVIVNGLVLASDGQKMSKRKKNYPDPVSIIQKYGADALRLYLINSPVVRAENLRFKEEGVRDVLKDVLLPWYNAYRFLIQNVLRLQKEEEIEFLYNENTVRESPNITDRWILSFMQSLIGFFETEMAAYRLYTVVPRLVKFVDILTNWYVRMNRRRLKGENGMEDCVMALETLFSVLLSLCRLMAPYTPFLTELMYQNLKVLIDPVSVQDKDTLSIHYLMLPRVREELIDKKTESAVSQMQSVIELGRVIRDRKTIPIKYPLKEIVVIHQDPEALKDIKSLEKYIIEELNVRKVTLSTDKNKYGIRLRAEPDHMVLGKRLKGAFKAVMTSIKQLSSEELEQFQKTGTIVVEGHELHDEDIRLMYTFDQATGGTAQFEAHSDAQALVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSEGTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVLIQEKTQLKGSELEITLTRGSSLPGPACAYVNLNICANGSEQGGVLLLENPKGDNRLDLLKLKSVVTSIFGVKNTELAVFHDETEIQNQTDLLSLSGKTLCVTAGSAPSLINSSSTLLCQYINLQLLNAKPQECLMGTVGTLLLENPLGQNGLTHQGLLYEAAKVFGLRSRKLKLFLNETQTQEITEDIPVKTLNMKTVYVSVLPTTADF 73CB3967A6868005 syep_human Bifunctional glutamate/proline--tRNA ligase EPRS1 Bifunctional aminoacyl-tRNA synthetase Cell proliferation-inducing gene 32 protein EPRS GLNS Glutamatyl-prolyl-tRNA synthetase PARS QARS QPRS PIG32 protein protein human Homo sapiens Human MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY DIP protein P07814 original sequence version: 204 DIP protein P07814 original sequence version: 155 DIP protein P07814 original sequence version: 159 DIP protein P07814 original sequence version: 122 2CE4311076719403 sylm_yeast Leucine--tRNA ligase, mitochondrial NAM2 MSL1 YLR382C L3502.6 Leucyl-tRNA synthetase protein protein yeast Saccharomyces cerevisiae Baker's yeast MLSRPSSRFLSTKRGPGPAVKKLIAIGEKWKQKTTRGLPKQDTLNSGSKYILCQFPYPSGALHIGHLRVYVISDSLNRFYKQKGYNVIHPMGWDAFGLPAENAAIERSINPAIWTRDNIAKMKQQMQSMLANFDWDREITTCDPEYYKFTQWIFLKLFENGLAYRKEAEINWDPVDMTVLANEQVDAQGRSWRSGAIVEKKQLKQWFLGITKFAPKLKKHLNQLKDWPSNVKQMQKNWIGESVGAELVFKVADPKFENLIVFTTRPETLFAVQYVALALDHPIVQKYCEEMPDLKEFIQKSDQLPNDTKEGFQLPNIKAVNPLTKEEVPIFAAPYVVSSYGSAPSAVMGCPGHDNRDFEFWQTNCPGEHIKTCIAPFFDDASKVTEQERQRIIDTVPFTSTDGVLTKECGEHSGVLTVVARKSIMGMLNSEGLSKSVVRYKIRDWLISRQRYWGTPIPIIHCDNCGPVPVPESDLPVKLPELEGLDTKGNPLSTIDEFVNVACPSCGSPAKRETDTMDTFIDSSWYYFRFLDPKNTSKPFDREIASKNMPVDIYIGGVEHAILHLLYSRFIAKFLGSINAWSDPAGIFEPFKKLVTQGMVQGKTYVDPDSGKFLKPDELTFVNDSPDGNTVIIKSNGKVPVVSYEKMSKSKYNGADPNECILRHGPDATRAHILFQSPIADALNWDESKIVGIERWLQKVLHLTKNILSLEKDLAISKDYKTPTDLNDAEVKFHNDFQRFLKSITESFEVNLSLNTVISDYMKLTNILESALKKGEVRNEMIVQNLQKLVTVIYPAVPSISEEAAEMINSQMEWNQYRWPEVERTTESKFKKFQIVVNGRVKFMYTADKNFLKLGRDAVIETLMNLPEGRMYLMNKKIKKFVMKFNVISFLFHK 3862CEFFFB10B262 mbi4_yeast Intron-encoded RNA maturase bI4 RNA maturase SCbI4 BI4 SCBI4 Q0120 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAFRKSNVYLSLVNSYIIDSPQPSSINYWWNMGSLLGLCLVIQIVTGIFMAMHYSSNIELAFSSVEHIMRDVHNGYILRYLHANGASFFFMVMFMHMAKGLYYGSYRSPRVTLWNVGVIIFILTIATAFLGYCCVYGQMSHWGATVITNLFSAIPFVGNDIVSWLWGGFSVSNPTIQRFFALHYLVPFIIAAMVIMHLMALHIHGSSNPLGITGNLDRIPMHSYFIFKDLVTVFLFMLILALFVFYSPNTLGQNMALLLITYVINILCAVCWKSLFIKYQWKIYNKTTYYFIIQNILNTKQLNNFVLKFNWTKQYNKMNIVSDLFNPNRVKYYYKEDNQQVTNMNSSNTHLTSNKKNLLVDTSETTRTTKNKFNYLLNIFNMKKMNQIITKRHYSIYKDSNIRFNQWLAGLIDGDGYFCITKNKYASCEITVELKDEKMLRQIQDKFGGSVKLRSGVKAIRYRLQNKEGMIKLINAVNGNIRNSKRLVQFNKVCILLNIDFKEPIKLTKDNAWFMGFFDADGTINYYYSGKLKIRPQLTISVTNKYLHDVEYYREVFGGNIYFDKAKNGYFKWSINNKELHNIFYTYNKSCPSKSNKGKRLFLIDKFYYLYDLLAFKAPHNTALYKAWLKFNEKWNNN E0D89849FF75C858 bi4_intron_yeast_mrna-nam2-1 3 4 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig. 3 bi4_intron_yeast_mrna-bi4-1 3 4 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Fig. 3 bi4_intron_yeast_mrna-nam2-2 2 8 unspecified role unspecified role bait bait 4 unspecified role unspecified role ancillary ancillary 7 unspecified role unspecified role prey prey physical association physical association Fig. 5 bi4_intron_yeast_mrna-bi4-2 2 7 unspecified role unspecified role bait bait 4 unspecified role unspecified role ancillary ancillary 8 unspecified role unspecified role prey prey physical association physical association Fig. 5 eprs-iars-1 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig. 5 bi4-nam2-1 1 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait 8 unspecified role unspecified role bait bait physical association physical association Fig. 7 bi4_intron_yeast_mrna-bi4-6 1 7 unspecified role unspecified role bait bait 4 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Fig. 7