DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1. Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1. Nature 1999 imex curation Qin H., Srinivasula SM., Wu G., Fernandes-Alnemri T., Alnemri ES., Shi Y. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Qin H., Srinivasula SM., Wu G., Fernandes-Alnemri T., Alnemri ES., Shi Y. DIP original experiment: DIP-265122X Nature 1999 IMEx Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1. Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1. Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1. Nature 1999 imex curation Qin H., Srinivasula SM., Wu G., Fernandes-Alnemri T., Alnemri ES., Shi Y. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Qin H., Srinivasula SM., Wu G., Fernandes-Alnemri T., Alnemri ES., Shi Y. DIP original experiment: DIP-265120X Nature 1999 IMEx Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1. Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1. Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1. Nature 1999 imex curation Qin H., Srinivasula SM., Wu G., Fernandes-Alnemri T., Alnemri ES., Shi Y. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Qin H., Srinivasula SM., Wu G., Fernandes-Alnemri T., Alnemri ES., Shi Y. DIP original experiment: DIP-265121X Nature 1999 IMEx Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1. apaf_human Apoptotic protease-activating factor 1 APAF1 KIAA0413 protein protein human Homo sapiens Human MDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVVSSSSGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTEKNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINKKNITNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQEDMEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYCDFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRLQLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIFQSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHDATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGDDNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAGGYIKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQTLE DIP protein O14727 original sequence version: 177 DIP protein O14727 original sequence version: 127 0750D05817AC9B3B casp9_human Caspase-9 ICE-like apoptotic protease 6 Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 CASP9 MCH6 protein protein human Homo sapiens Human MDEADRRLLRRCRLRLVEELQVDQLWDALLSRELFRPHMIEDIQRAGSGSRRDQARQLIIDLETRGSQALPLFISCLEDTGQDMLASFLRTNRQAAKLSKPTLENLTPVVLRPEIRKPEVLRPETPRPVDIGSGGFGDVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS DIP protein P55211 original sequence version: 174 DIP protein P55211 original sequence version: 167 DIP protein P55211 original sequence version: 124 DIP protein P55211 original sequence version: 125 DIP protein P55211 original sequence version: 123 DIP protein P55211 original sequence version: 153 78E0180DF2A3BDD2 apaf1-casp9-5 2 5 unspecified role unspecified role unspecified role unspecified role 1-112 Binding region 1-112 Binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role 1-97 Binding region 1-97 Binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction apaf1-casp9-6 1 5 unspecified role unspecified role unspecified role unspecified role 1-112 Binding region 1-112 Binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain P55211:p.Arg11Ala R11A Mutatiion mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R11A Mutatiion'. Wed May 02 18:16:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg11ala' Tue Jun 30 17:15:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg11Ala' P55211:p.Arg13Ala R13A Mutatiion mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R13A Mutatiion'. Wed May 02 16:46:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg13ala' Tue Jun 30 18:55:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg13Ala' P55211:p.Arg52Ala R52A Mutatiion mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R52A Mutatiion'. Wed May 02 18:36:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg52ala' Tue Jun 30 18:56:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg52Ala' P55211:p.Arg56Ala R56A Mutatiion mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R56A Mutatiion'. Wed May 02 19:18:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg56ala' Tue Jun 30 20:17:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg56Ala' ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role 1-97 Binding region 1-97 Binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction apaf1-casp9-7 3 4 unspecified role unspecified role unspecified role unspecified role 1-97 Binding region 1-97 Binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain O14727:p.Asp27Ala D27A Mutatiion mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D27A Mutatiion'. Wed May 02 15:39:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp27ala' Tue Jun 30 17:28:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp27Ala' O14727:p.Ser31Ala S31A Mutatiion mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:13:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser31ala' Tue Jun 30 18:42:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser31Ala' O14727:p.Glu40Ala E40A Mutatiion mutation disrupting mutation disrupting interaction mutation disrupting substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E40A Mutatiion'. Wed May 02 20:27:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu40ala' Tue Jun 30 18:52:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu40Ala' ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role 1-112 Binding region 1-112 Binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction