IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2. Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2. The EMBO journal 1998 imex curation Gerber A., Grosjean H., Melcher T., Keller W., Gerber A., Grosjean H., Melcher T., Keller W. Keller2@ubaclu.unibas.ch 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro deamination deamination assay aminase assay deamination nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Gerber A., Grosjean H., Melcher T., Keller W., Gerber A., Grosjean H., Melcher T., Keller W. Keller2@ubaclu.unibas.ch Accepted 2016-MAY-24 AT 08:18 BST AT 08:18 BST by ORCHARD The EMBO journal 1998 imex curation Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2. Tad1p, a yeast tRNA-specific adenosine deaminase, is related to the mammalian pre-mRNA editing enzymes ADAR1 and ADAR2. The EMBO journal 1998 imex curation Gerber A., Grosjean H., Melcher T., Keller W., Gerber A., Grosjean H., Melcher T., Keller W. Keller2@ubaclu.unibas.ch 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro deamination deamination assay aminase assay deamination weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Gerber A., Grosjean H., Melcher T., Keller W., Gerber A., Grosjean H., Melcher T., Keller W. To detect the Inosine, the tRNA was digested with P1 nuclease and the reaction products were separated by TLC Keller2@ubaclu.unibas.ch Accepted 2016-MAY-24 AT 08:18 BST AT 08:18 BST by ORCHARD The EMBO journal 1998 imex curation trna ala agc-1 trna transfer rna transfer ribonucleic acid tRNA transfer RNA trna bommo Bombyx mori Silk moth AACGGGGGCGUAGCUCAGAUGGUAGAGCGCUCGCUUAGCAUGCGAGAGGUACCGGGAUCGAUACCCGGCGCCUCCAAUCUCGACUGUCUUUUUCCA trna ala agc tRNA ala AGC yeast trna transfer rna transfer ribonucleic acid tRNA transfer RNA trna yeast Saccharomyces cerevisiae Baker's yeast GGGCGUGUGGCGUAGUCGGUAGCGCGCUCCCUUAGCAUGGGAGAGGUCUCCGGUUCGAUUCCGGACUCGUCCA tad1_yeast tRNA-specific adenosine deaminase 1 TAD1 tRNA-specific adenosine-37 deaminase HRA400 YGL243W protein protein yeast Saccharomyces cerevisiae Baker's yeast MVSCQGTRPCIVNLLTMPSEDKLGEEISTRVINEYSKLKSACRPIIRPSGIREWTILAGVAAINRDGGANKIEILSIATGVKALPDSELQRSEGKILHDCHAEILALRGANTVLLNRIQNYNPSSGDKFIQHNDEIPARFNLKENWELALYISRLPCGDASMSFLNDNCKNDDFIKIEDSDEFQYVDRSVKTILRGRLNFNRRNVVRTKPGRYDSNITLSKSCSDKLLMKQRSSVLNCLNYELFEKPVFLKYIVIPNLEDETKHHLEQSFHTRLPNLDNEIKFLNCLKPFYDDKLDEEDVPGLMCSVKLFMDDFSTEEAILNGVRNGFYTKSSKPLRKHCQSQVSRFAQWELFKKIRPEYEGISYLEFKSRQKKRSQLIIAIKNILSPDGWIPTRTDDVK 2AA9304C549026FF tad1-1 1 5 enzyme enzyme neutral component neutral component tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 4 enzyme target enzyme target substrate substrate neutral component neutral component Inosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined deamination deamination reaction deamination Fig 3 tad1-trna ala agc-1 2 5 enzyme enzyme neutral component neutral component tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris 4 enzyme target enzyme target substrate substrate neutral component neutral component Inosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain deamination deamination reaction deamination Fig 2, Table 1 tad1-trna ala agc-2 2 3 enzyme target enzyme target substrate substrate neutral component neutral component Inosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain g48a required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain G A label 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 enzyme enzyme neutral component neutral component tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range picpa Pichia pastoris (Yeast) Yeast Pichia pastoris deamination deamination reaction deamination Table 1