IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer. Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer. Cancer cell 2016 imex curation Brisson L, Brisson L., Bański P., Sboarina M., Dethier C., Danhier P., Fontenille MJ., Van Hée VF., Vazeille T., Tardy M., Falces J., Bouzin C., Porporato PE., Frédérick R., Michiels C., Copetti T., Sonveaux P. Cancer - Interactions investigated in the context of cancer pierre.sonveaux@uclouvain.be 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions human-siha Homo sapiens SiHa cervical carcinoma cell line siha SiHa cells huamn cervical carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Brisson L, Brisson L., Bański P., Sboarina M., Dethier C., Danhier P., Fontenille MJ., Van Hée VF., Vazeille T., Tardy M., Falces J., Bouzin C., Porporato PE., Frédérick R., Michiels C., Copetti T., Sonveaux P. Cancer - Interactions investigated in the context of cancer The antibody used to detect V-ATPase A1 in Western Blotting is not stated;however the polyclonal antibody (H140, Santa Cruz) used in PLA is stated to possibly cross-react with ATP6V0A4. pierre.sonveaux@uclouvain.be Accepted 2017-JAN-09 AT 14:44 GMT AT 14:44 GMT by ORCHARD Cancer cell 2016 imex curation Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer. Lactate Dehydrogenase B Controls Lysosome Activity and Autophagy in Cancer. Cancer cell 2016 imex curation Brisson L, Brisson L., Bański P., Sboarina M., Dethier C., Danhier P., Fontenille MJ., Van Hée VF., Vazeille T., Tardy M., Falces J., Bouzin C., Porporato PE., Frédérick R., Michiels C., Copetti T., Sonveaux P. Cancer - Interactions investigated in the context of cancer pierre.sonveaux@uclouvain.be 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions human-siha Homo sapiens SiHa cervical carcinoma cell line siha SiHa cells huamn cervical carcinoma cells pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Brisson L, Brisson L., Bański P., Sboarina M., Dethier C., Danhier P., Fontenille MJ., Van Hée VF., Vazeille T., Tardy M., Falces J., Bouzin C., Porporato PE., Frédérick R., Michiels C., Copetti T., Sonveaux P. Cancer - Interactions investigated in the context of cancer The polyclonal antibody (H140, Santa Cruz) targeted to A1 subunit of V-ATPase is stated to also react with ATP6V0A4. However the ATP6V0A1 immunogen sequence has only 54% homology with the ATP6V0A4 so cross reactivity may not be significant. pierre.sonveaux@uclouvain.be Accepted 2017-JAN-09 AT 14:44 GMT AT 14:44 GMT by ORCHARD Cancer cell 2016 imex curation vpp1_human V-type proton ATPase 116 kDa subunit a1 ATP6V0A1 ATP6N1 ATP6N1A VPP1 Vacuolar proton translocating ATPase 116 kDa subunit a isoform 1 Clathrin-coated vesicle/synaptic vesicle proton pump 116 kDa subunit Vacuolar proton pump subunit 1 Vacuolar adenosine triphosphatase subunit Ac116 protein protein human Homo sapiens Human MGELFRSEEMTLAQLFLQSEAAYCCVSELGELGKVQFRDLNPDVNVFQRKFVNEVRRCEEMDRKLRFVEKEIRKANIPIMDTGENPEVPFPRDMIDLEANFEKIENELKEINTNQEALKRNFLELTELKFILRKTQQFFDEMADPDLLEESSSLLEPSEMGRGTPLRLGFVAGVINRERIPTFERMLWRVCRGNVFLRQAEIENPLEDPVTGDYVHKSVFIIFFQGDQLKNRVKKICEGFRASLYPCPETPQERKEMASGVNTRIDDLQMVLNQTEDHRQRVLQAAAKNIRVWFIKVRKMKAIYHTLNLCNIDVTQKCLIAEVWCPVTDLDSIQFALRRGTEHSGSTVPSILNRMQTNQTPPTYNKTNKFTYGFQNIVDAYGIGTYREINPAPYTIITFPFLFAVMFGDFGHGILMTLFAVWMVLRESRILSQKNENEMFSTVFSGRYIILLMGVFSMYTGLIYNDCFSKSLNIFGSSWSVRPMFTYNWTEETLRGNPVLQLNPALPGVFGGPYPFGIDPIWNIATNKLTFLNSFKMKMSVILGIIHMLFGVSLSLFNHIYFKKPLNIYFGFIPEIIFMTSLFGYLVILIFYKWTAYDAHTSENAPSLLIHFINMFLFSYPESGYSMLYSGQKGIQCFLVVVALLCVPWMLLFKPLVLRRQYLRRKHLGTLNFGGIRVGNGPTEEDAEIIQHDQLSTHSEDADEPSEDEVFDFGDTMVHQAIHTIEYCLGCISNTASYLRLWALSLAHAQLSEVLWTMVIHIGLSVKSLAGGLVLFFFFTAFATLTVAILLIMEGLSAFLHALRLHWVEFQNKFYSGTGFKFLPFSFEHIREGKFEE 80E4842CF6ADEE44 ldhb_human L-lactate dehydrogenase B chain LDHB LDH heart subunit Renal carcinoma antigen NY-REN-46 protein protein human Homo sapiens Human MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL 3AD605DEED0D54A2 aey5zh-1 2 3 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 5A atp6v0a1-ldhb-1 1 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 5B