IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro gtpase assay gtpase assay GTPase gtp hydrolisis gtp hydrolisis GTPase predetermined predetermined participant predetermined Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease cannot find aas for the construct used, so far Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. dls dynamic light scattering dls predetermined predetermined participant predetermined Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. Cross linking agent was DSS (disuccinimidyl suberate). After Cross linking samples were precipitated and proteolysis carried out, the resulting peptide mixtures were separated by SEC, followed by MS. Only High confidence results have been entered. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. Cross linking agent was DSS (disuccinimidyl suberate). Samples were separated on an SDS-PAGE (NuPage) gel. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy antibody detection identification by antibody antibody detection Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. A Gold-labeled secondary antibody was used to bind to primary antibodies. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. Cross linking agent was DSG (disuccinimidyl glutarate). After Cross linking samples were precipitated and proteolysis carried out, the resulting peptide mixtures were separated by SEC, followed by MS. Only High confidence results have been entered. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay tag visualisation tag visualisation Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] bn-page blue native page bn-page western blot western blot Immuno blot Immuno blot Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. Cross linking agent was DSS (disuccinimidyl suberate). Samples were separated on an BN-PAGE gel. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts Structural model of the dimeric Parkinson’s protein LRRK2 reveals a compact architecture involving distant interdomain contacts PNAS 2016 imex curation Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. bn-page blue native page bn-page weight by comassie molecular weight estimation by coomasie staining weight by comassie Giambattista Guaitoli, Giambattista Guaitoli Parkinsons - Interactions investigated in the context of Parkinsons disease Fig S4 says "rat brain" - see Data -Proc comment. The authors have stated that:- "The material used for EM came from transgenic mice, expressing FLAG-tagged murine forms of WT Lrrk2 as described in Ref. 29." iban.ubarretxena@mssm.edu,a.kortholt@rug.nl,johannes.gloeckner@dzne.de Accepted 2016-AUG-16 AT 11:13 BST AT 11:13 BST by ORCHARD PNAS 2016 imex curation gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 lrrk2_mouse Leucine-rich repeat serine/threonine-protein kinase 2 Lrrk2 protein protein mouse Mus musculus Mouse MASGACQGCEEEEEEEALKKLIVRLNNVQEGKQIETLLQLLEDMLVFTYSDRASKLFEDKNFHVPLLIVLDSYMRVASVQQAGWSLLCKLIEVCPGTLQSLIGPQDIGNDWEVLGIHRLILKMLTVHHANVNLSIVGLKALDLLLDSGKLTLLILDEECDIFLLIFDAMHRYSANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYTILLSTFGSFRRDKEIVYHVLCCLHSLAVTCSNVEVLMSGNVRCYNLVVEAMKAFPTNENIQEVSCSLFQKLTLGNFFNILVLNEVHVFVVKAVRQYPENAALQISALSCLALLTETIFLNQDLEERSETQEQSEEEDSEKLFWLEPCYKALVRHRKDKHVQEAACWALNNLLMYQNSLHEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAAAHALSTLLEQNVNFRKILLAKGVYLNVLELMQKHAHAPEVAESGCKMLSHLFEGSNPSLDTMAAVVPKILTVMKAHGTSLSVQLEALRAILHFVVPGLLEESREDSQCRPNVLRKQCFRTDIHKLVLVALNRFIGNPGIQKCGLKVISSLAHLPDATETLSLQGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIGTGHLLAKILASTLQRFKDVAEVQTTGLQTTLSILELSVSFSKLLVHYSFDVVIFHQMSSSVVEQKDEQFLNLCCKCFAKVAVDDELKNTMLERACDQNNSIMVECLLLLGADANQVKGATSLIYQVCEKESSPKLVELLLNGGCREQDVRKALTVSIQKGDSQVISLLLRKLALDLANNSICLGGFGIGKIDPSWLGPLFPDKSSNLRKQTNTGSVLARKVLRYQMRNTLQEGVASGSDGKFSEDALAKFGEWTFIPDSSMDSVFGQSDDLDSEGSESSFLVKRKSNSISVGEVYRDLALQRCSPNAQRHSNSLGPVFDHEDLLRRKRKILSSDESLRSSRLPSHMRQSDSSSSLASEREHITSLDLSANELKDIDALSQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLKCLIHLDLHSNKFTSFPSFVLKMPRITNLDASRNDIGPTVVLDPAMKCPSLKQLNLSYNQLSSIPENLAQVVEKLEQLLLEGNKISGICSPLSLKELKILNLSKNHIPSLPGDFLEACSKVESFSARMNFLAAMPALPSSITSLKLSQNSFTCIPEAIFSLPHLRSLDMSHNNIECLPGPAHWKSLNLRELIFSKNQISTLDFSENPHVWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHVGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKMKKPELGMQGATVGIDVRDWSIQIRGKRRKDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACISKITKELLNKRGFPTIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQPVVGQLIPDCYVELEKIILSERKAVPTEFPVINRKHLLQLVNEHQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQILTVKVDGCLKHPKGIISRRDVEKFLSKKKRFPKNYMMQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEEAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLHDIWTTGSRIMEGLRFPNEFDELAIQGKLPDPVKEYGCAPWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHILIPKNIIVECMVATNLNSKSATLWLGCGNTEKGQLSLFDLNTERYSYEEVADSRILCLALVHLAAEKESWVVCGTQSGALLVINVEEETKRHTLEKMTDSVTCLHCNSLAKQSKQSNFLLVGTADGNLMIFEDKAVKCKGAAPLKTLHIGDVSTPLMCLSESLNSSERHITWGGCGTKVFSFSNDFTIQKLIETKTNQLFSYAAFSDSNIIALAVDTALYIAKKNSPVVEVWDKKTEKLCELIDCVHFLKEVMVKLNKESKHQLSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHNFCDSVRAMATAQLGSLKNVMLVLGYKRKSTEGIQEQKEIQSCLSIWDLNLPHEVQNLEKHIEVRTELADKMRKTSVE DIP protein Q5S006 original sequence version: 57 706E25C173E36F98 moes_human Moesin Membrane-organizing extension spike protein MSN protein protein human Homo sapiens Human MPKTISVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWFFGLQYQDTKGFSTWLKLNKKVTAQDVRKESPLLFKFRAKFYPEDVSEELIQDITQRLFFLQVKEGILNDDIYCPPETAVLLASYAVQSKYGDFNKEVHKSGYLAGDKLLPQRVLEQHKLNKDQWEERIQVWHEEHRGMLREDAVLEYLKIAQDLEMYGVNYFSIKNKKGSELWLGVDALGLNIYEQNDRLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILALCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQMERAMLENEKKKREMAEKEKEKIEREKEELMERLKQIEEQTKKAQQELEEQTRRALELEQERKRAQSEAEKLAKERQEAEEAKEALLQASRDQKKTQEQLALEMAELTARISQLEMARQKKESEAVEWQQKAQMVQEDLEKTRAELKTAMSTPHVAEPAENEQDEQDENGAEASADLRADAMAKDRSEEERTTEAEKNERVQKHLKALTSELANARDESKKTANDMIHAENMRLGRDKYKTLRQIRQGNTKQRIDEFESM DIP protein P26038 original sequence version: 168 865A6C5CB14AE586 lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE DIP protein Q5S007 original sequence version: 36 DIP protein Q5S007 original sequence version: 68 DIP protein Q5S007 original sequence version: 64 26142A0CECBBC3F4 q8kc98_chlte CT1526 protein protein chlte Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / TLS) MSDLDVIRQIEQELGMQLEPVDKLKWYSKGYKLDKDQRVTAIGLYDCGSDTLDRIIQPLESLKSLSELSLSSNQITDISPLASLNSLSMLWLDRNQITDIAPLASLNSLSMLWLFGNKISDIAPLESLKSLTELQLSSNQITDIAPLASLKSLTELSLSGNNISDIAPLESLKSLTELSLSSNQITDIAPLASLKSLTELSLSSNQISDIAPLESLKSLTELQLSRNQISDIAPLESLKSLTELQLSSNQITDIAPLASLKSLTELQLSRNQISDIAPLESLNSLSKLWLNGNQITDIAPLASLNSLTELELSSNQITDIAPLASLKSLSTLWLSSNQISDIAPLASLESLSELSLSSNQISDISPLASLNSLTGFDVRRNPIKRLPETITGFDMEILWNDFSSSGFITFFDNPLESPPPEIVKQGKEAVRQYFQSIEEARSKGEALVHLQEIKVHLIGDGMAGKTSLLKQLIGETFDPKESQTHGLNVVTKQAPNIKGLENDDELKECLFHFWDFGGQEIMHASHQFFMTRSSVYMLLLDSRTDSNKHYWLRHIEKYGGKSPVIVVMNKIDENPSYNIEQKKINERFPAIENRFHRISCKNGDGVESIAKSLKSAVLHPDSIYGTPLAPSWIKVKEKLVEATTAQRYLNRTEVEKICNDSGITDPGERKTLLGYLNNLGIVLYFEALDLSEIYVLDPHWVTIGVYRIINSSKTKNGHLNTSALGYILNEEQIRCDEYDPAKNNKFTYTLLEQRYLLDIMKQFELCYDEGKGLFIIPSNLPTQIDNEPEITEGEPLRFIMKYDYLPSTIIPRLMIAMQHQILDRMQWRYGMVLKSQDHEGALAKVVAETKDSTITIAIQGEPRCKREYLSIIWYEIKKINANFTNLDVKEFIPLPGHPDELVEYKELLGLEKMGRDEYVSGKLEKVFSVSKMLDSVISKEERNKERLMGDINIKLENIGNPTIPIHQQVEVNVSQETVQHVENLQGFFENLKADILREAELEIDDPKERKRLANELELAENAITKMDAAVKSGKNKLKPDVKDRLGEFIDNLANENSRLRKGIALVMNGAEKVQKLARYYNNVAPFFDLPSVPPVLLGKEKT mint A8A3B242A43894A8 lrrk2-37 12 16 unspecified role unspecified role self self n_terminal_strep_flag_tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range self interaction self interaction 1A, Supp 2 C lrrk2-30 1 13 enzyme target enzyme target substrate substrate neutral component neutral component region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 enzyme enzyme neutral component neutral component n_terminal_strep_flag_tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. gtpase reaction gtpase reaction 1B gtp-lrrk2-7 9 15 enzyme target enzyme target substrate substrate neutral component neutral component lrrktide_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 16 enzyme enzyme neutral component neutral component n_terminal_strep_flag_tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 1B GDP may be present, but role is not clear, and the reaction occurs in absence of GDP - so not entered. lrrk2-31 4 16 unspecified role unspecified role self self purified purified n_terminal_strep_flag_tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range self interaction self interaction Supp 2A lrrk2-32 6 16 unspecified role unspecified role self self purified purified n_terminal_strep_flag_tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. self interaction self interaction 2B lrrk2-33 11 16 unspecified role unspecified role self self purified purified n_terminal_strep_flag_tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. self interaction self interaction 2C lrrk2-34 5 16 unspecified role unspecified role neutral component neutral component purified purified lys951 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys951 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1463 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1471 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1463 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1502 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain n_terminal_strep_flag_tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range wd40_domain sufficient to bind sufficient binding region sufficient to bind range range range range kinase_domain sufficient to bind sufficient binding region sufficient to bind range range range range lys825 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1502 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1539 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2030 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2478 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2415 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys951 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2515 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2520 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys951 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1463 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1539 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1356 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys951 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1623 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain kinase_domain sufficient to bind sufficient binding region sufficient to bind range range range range lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 16 unspecified role unspecified role neutral component neutral component purified purified n_terminal_strep_flag_tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ankyrin_domain sufficient to bind sufficient binding region sufficient to bind range range range range ankyrin_domain sufficient to bind sufficient binding region sufficient to bind range range range range lys756 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys773 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys947 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys947 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys951 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2030 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lrr_domains sufficient to bind sufficient binding region sufficient to bind range range range range lys639 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys739 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1996 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2148 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2478 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2078 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys756 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys756 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys825 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1460 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1460 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1471 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1499 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1499 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1499 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1502 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1539 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1539 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1620 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1833 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1833 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1952 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 42 99 100 43 85 44 102 45 103 46 87 47 104 48 105 49 107 50 108 51 89 52 54 81 90 55 93 56 112 57 58 114 94 59 95 60 96 61 97 62 98 63 64 91 65 92 66 84 67 101 86 68 69 88 70 106 71 109 110 72 111 73 113 74 75 115 116 76 82 77 78 83 self interaction self interaction 3, Supp 3, Dataset S1 I count 33 X links in both Table S1 and here. lrrk2-35 8 16 unspecified role unspecified role putative self putative self wd40_domain sufficient to bind sufficient binding region sufficient to bind range range range range kinase_domain sufficient to bind sufficient binding region sufficient to bind range range range range lys639 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys951 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys951 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2520 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain n_terminal_strep_flag_tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range lrr_domains sufficient to bind sufficient binding region sufficient to bind range range range range lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys951 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2520 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1249 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1316 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1910 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 16 unspecified role unspecified role putative self putative self lrr_domains sufficient to bind sufficient binding region sufficient to bind range range range range lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys756 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2030 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ankyrin_domain sufficient to bind sufficient binding region sufficient to bind range range range range lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys951 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2030 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys831 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1952 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys1952 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2030 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2030 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys2030 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys947 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys963 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys756 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain n_terminal_strep_flag_tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ankyrin_domain sufficient to bind sufficient binding region sufficient to bind range range range range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 119 163 120 142 144 121 148 122 159 123 124 149 160 125 147 127 128 145 153 129 161 130 131 151 132 143 154 133 134 146 135 150 152 136 158 137 157 138 139 156 140 155 self interaction self interaction Dataset S2 q8kc98_chlte-q8kc98_chlte-2 2 17 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Table S1 2.3A R work 0.214 R free 0.261 q8kc98_chlte-q8kc98_chlte-1 3 17 unspecified role unspecified role neutral component neutral component LRR-roc-cor sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role neutral component neutral component LRR-roc-cor sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 169 171 direct interaction direct interaction Dataset S9, supp Fig 10. 2345w-1 10 14 unspecified role unspecified role neutral component neutral component purified purified n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp 4A lrrk2-36 7 14 unspecified role unspecified role neutral component neutral component purified purified n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp 4C