IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Cancer cell 2016 imex curation Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr Accepted 2016-SEP-02 AT 13:36 BST AT 13:36 BST by ORCHARD Cancer cell 2016 imex curation added to Fig 3B interaction; No! I fell into this trap before- one protein is on 2 lines:-"Guanine nucleotide-binding protein subunit beta-2-like 1" -so there are only 20 proteins!; comment deleted and 2 extra interactions added with "comment".; done; done Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Cancer cell 2016 imex curation Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? HA15 was detected/visualized after excitation at 405 nm. It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr Accepted 2016-SEP-02 AT 13:36 BST AT 13:36 BST by ORCHARD Cancer cell 2016 imex curation added to Fig 3B interaction; No! I fell into this trap before- one protein is on 2 lines:-"Guanine nucleotide-binding protein subunit beta-2-like 1" -so there are only 20 proteins!; comment deleted and 2 extra interactions added with "comment".; done; done Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Cancer cell 2016 imex curation Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down sequence tag sequence tag identification sequence tag Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr Accepted 2016-SEP-02 AT 13:36 BST AT 13:36 BST by ORCHARD Cancer cell 2016 imex curation added to Fig 3B interaction; No! I fell into this trap before- one protein is on 2 lines:-"Guanine nucleotide-binding protein subunit beta-2-like 1" -so there are only 20 proteins!; comment deleted and 2 extra interactions added with "comment".; done; done Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Cancer cell 2016 imex curation Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions human-melanoma Human melanoma melanoma Melanoma fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr Accepted 2016-SEP-02 AT 13:36 BST AT 13:36 BST by ORCHARD Cancer cell 2016 imex curation added to Fig 3B interaction; No! I fell into this trap before- one protein is on 2 lines:-"Guanine nucleotide-binding protein subunit beta-2-like 1" -so there are only 20 proteins!; comment deleted and 2 extra interactions added with "comment".; done; done Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Cancer cell 2016 imex curation Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions human-melanoma Human melanoma melanoma Melanoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr Accepted 2016-SEP-02 AT 13:36 BST AT 13:36 BST by ORCHARD Cancer cell 2016 imex curation added to Fig 3B interaction; No! I fell into this trap before- one protein is on 2 lines:-"Guanine nucleotide-binding protein subunit beta-2-like 1" -so there are only 20 proteins!; comment deleted and 2 extra interactions added with "comment".; done; done Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Cancer cell 2016 imex curation Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr Accepted 2016-SEP-02 AT 13:36 BST AT 13:36 BST by ORCHARD Cancer cell 2016 imex curation added to Fig 3B interaction; No! I fell into this trap before- one protein is on 2 lines:-"Guanine nucleotide-binding protein subunit beta-2-like 1" -so there are only 20 proteins!; comment deleted and 2 extra interactions added with "comment".; done; done Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Cancer cell 2016 imex curation Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro dsc differential scanning calorimetry dsc predetermined predetermined participant predetermined Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr Accepted 2016-SEP-02 AT 13:36 BST AT 13:36 BST by ORCHARD Cancer cell 2016 imex curation added to Fig 3B interaction; No! I fell into this trap before- one protein is on 2 lines:-"Guanine nucleotide-binding protein subunit beta-2-like 1" -so there are only 20 proteins!; comment deleted and 2 extra interactions added with "comment".; done; done Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Compounds Triggering ER Stress Exert Anti-Melanoma Effects and Overcome BRAF Inhibitor Resistance. Cancer cell 2016 imex curation Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr 15-Sep-2016: Contacted by IntAct-Help. Only protein-protein interactions human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cerezo M., Lehraiki A., Millet A., Rouaud F., Plaisant M., Jaune E., Botton T., Ronco C., Abbe P., Amdouni H., Passeron T., Hofman V., Mograbi B., Dabert-Gay AS., Debayle D., Alcor D., Rabhi N., Annicotte JS., Héliot L., Gonzalez-Pisfil M., Robert C., Moréra S., Virougoux A., Gual P., Ali MM., Bertolotto C., Hofman P., Ballotti R., Benhida R., Rocchi S. Cancer - Interactions investigated in the context of cancer As the authors have not replied to a request for information:- Results from a FRET experiment - Supplementary Figure 3A, have not been included as no cell line is stated here and associated references give several different cell lines. Interactions involving recombinant IRE1-alpha have not been entered as the species of origin is not given. cannot tell species of GFP-BiP - Origene= many species of BiP, and there is only a (human) "Recombinant BiP" given for ATPase assay, and BiP-NBD is Human. -species not given for Pull downs + other uses?. I have entered the BiP-NBD in fuzzy type as it is not directly quoted here, nor in the associated Addgene reference, but maybe not necessary to do so? It has been assumed that the protein BiP (GRP-78/HSPA5) is of Human origin in all constructs used; however this has not been confirmed by the authors. BiP-NBD has been assumed to be the "Nucleotide binding domain" amino acids 26-410 as given in the PDB entry 3iuc, referenced by Addgene plasmid 42447. benhida@unice.fr,srocchi@unice.fr Accepted 2016-SEP-02 AT 13:36 BST AT 13:36 BST by ORCHARD Cancer cell 2016 imex curation added to Fig 3B interaction; No! I fell into this trap before- one protein is on 2 lines:-"Guanine nucleotide-binding protein subunit beta-2-like 1" -so there are only 20 proteins!; comment deleted and 2 extra interactions added with "comment".; done; done anxa5_human Annexin A5 ANXA5 ANX5 ENX2 PP4 Annexin-5 Annexin V Lipocortin V Endonexin II Calphobindin I Placental anticoagulant protein I Placental anticoagulant protein 4 Thromboplastin inhibitor Vascular anticoagulant-alpha Anchorin CII protein protein human Homo sapiens Human MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD 45E14E3964BA4D1A e2ak3_human Eukaryotic translation initiation factor 2-alpha kinase 3 PRKR-like endoplasmic reticulum kinase Pancreatic eIF2-alpha kinase EIF2AK3 PEK PERK protein protein human Homo sapiens Human MERAISPGLLVRALLLLLLLLGLAARTVAAGRARGLPAPTAEAAFGLGAAAAPTSATRVPAAGAVAAAEVTVEDAEALPAAAGEQEPRGPEPDDETELRPRGRSLVIISTLDGRIAALDPENHGKKQWDLDVGSGSLVSSSLSKPEVFGNKMIIPSLDGALFQWDQDRESMETVPFTVESLLESSYKFGDDVVLVGGKSLTTYGLSAYSGKVRYICSALGCRQWDSDEMEQEEDILLLQRTQKTVRAVGPRSGNEKWNFSVGHFELRYIPDMETRAGFIESTFKPNENTEESKIISDVEEQEAAIMDIVIKVSVADWKVMAFSKKGGHLEWEYQFCTPIASAWLLKDGKVIPISLFDDTSYTSNDDVLEDEEDIVEAARGATENSVYLGMYRGQLYLQSSVRISEKFPSSPKALESVTNENAIIPLPTIKWKPLIHSPSRTPVLVGSDEFDKCLSNDKFSHEEYSNGALSILQYPYDNGYYLPYYKRERNKRSTQITVRFLDNPHYNKNIRKKDPVLLLHWWKEIVATILFCIIATTFIVRRLFHPHPHRQRKESETQCQTENKYDSVSGEANDSSWNDIKNSGYISRYLTDFEPIQCLGRGGFGVVFEAKNKVDDCNYAIKRIRLPNRELAREKVMREVKALAKLEHPGIVRYFNAWLEAPPEKWQEKMDEIWLKDESTDWPLSSPSPMDAPSVKIRRMDPFATKEHIEIIAPSPQRSRSFSVGISCDQTSSSESQFSPLEFSGMDHEDISESVDAAYNLQDSCLTDCDVEDGTMDGNDEGHSFELCPSEASPYVRSRERTSSSIVFEDSGCDNASSKEEPKTNRLHIGNHCANKLTAFKPTSSKSSSEATLSISPPRPTTLSLDLTKNTTEKLQPSSPKVYLYIQMQLCRKENLKDWMNGRCTIEERERSVCLHIFLQIAEAVEFLHSKGLMHRDLKPSNIFFTMDDVVKVGDFGLVTAMDQDEEEQTVLTPMPAYARHTGQVGTKLYMSPEQIHGNSYSHKVDIFSLGLILFELLYPFSTQMERVRTLTDVRNLKFPPLFTQKYPCEYVMVQDMLSPSPMERPEAINIIENAVFEDLDFPGKTVLRQRSRSLSSSGTKHSRQSNNSHSPLPSN 95FEB5DAE8D3D452 actn1_human Alpha-actinin-1 ACTN1 Alpha-actinin cytoskeletal isoform Non-muscle alpha-actinin-1 F-actin cross-linking protein protein protein human Homo sapiens Human MDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNAWGCLEQVEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLRQKDYETATLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDNLGALTQKRREALERTEKLLETIDQLYLEYAKRAAPFNNWMEGAMEDLQDTFIVHTIEEIQGLTTAHEQFKATLPDADKERLAILGIHNEVSKIVQTYHVNMAGTNPYTTITPQEINGKWDHVRQLVPRRDQALTEEHARQQHNERLRKQFGAQANVIGPWIQTKMEEIGRISIEMHGTLEDQLSHLRQYEKSIVNYKPKIDQLEGDHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMNEFRASFNHFDRDHSGTLGPEEFKACLISLGYDIGNDPQGEAEFARIMSIVDPNRLGVVTFQAFIDFMSRETADTDTADQVMASFKILAGDKNYITMDELRRELPPDQAEYCIARMAPYTGPDSVPGALDYMSFSTALYGESDL 6DA3E4D1A0289519 1433e_human 14-3-3 protein epsilon YWHAE protein protein human Homo sapiens Human MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ DIP protein P62258 original sequence version: 130 DIP protein P62258 original sequence version: 132 DIP protein P62258 original sequence version: 91 07817CCBD1F75B26 atf6a_human Cyclic AMP-dependent transcription factor ATF-6 alpha Activating transcription factor 6 alpha ATF6 protein protein human Homo sapiens Human MGEPAGVAGTMESPFSPGLFHRLDEDWDSALFAELGYFTDTDELQLEAANETYENNFDNLDFDLDLMPWESDIWDINNQICTVKDIKAEPQPLSPASSSYSVSSPRSVDSYSSTQHVPEELDLSSSSQMSPLSLYGENSNSLSSAEPLKEDKPVTGPRNKTENGLTPKKKIQVNSKPSIQPKPLLLPAAPKTQTNSSVPAKTIIIQTVPTLMPLAKQQPIISLQPAPTKGQTVLLSQPTVVQLQAPGVLPSAQPVLAVAGGVTQLPNHVVNVVPAPSANSPVNGKLSVTKPVLQSTMRNVGSDIAVLRRQQRMIKNRESACQSRKKKKEYMLGLEARLKAALSENEQLKKENGTLKRQLDEVVSENQRLKVPSPKRRVVCVMIVLAFIILNYGPMSMLEQDSRRMNPSVSPANQRRHLLGFSAKEAQDTSDGIIQKNSYRYDHSVSNDKALMVLTEEPLLYIPPPPCQPLINTTESLRLNHELRGWVHRHEVERTKSRRMTNNQQKTRILQGALEQGSNSQLMAVQYTETTSSISRNSGSELQVYYASPRSYQDFFEAIRRRGDTFYVVSFRRDHLLLPATTHNKTTRPKMSIVLPAININENVINGQDYEVMMQIDCQVMDTRILHIKSSSVPPYLRDQQRNQTNTFFGSPPAATEATHVVSTIPESLQ DIP protein P18850 original sequence version: 117 DIP protein P18850 original sequence version: 127 DIP protein P18850 original sequence version: 130 5EBD08CF4121D41A rs4x_human 40S ribosomal protein S4, X isoform RPS4X CCG2 RPS4 SCAR Single copy abundant mRNA protein SCR10 Small ribosomal subunit protein eS4 protein protein human Homo sapiens Human MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEERDKRLAAKQSSG 87200E545A8958B0 ern1_human Serine/threonine-protein kinase/endoribonuclease IRE1 ERN1 Inositol-requiring protein 1 Ire1-alpha Endoplasmic reticulum-to-nucleus signaling 1 IRE1 protein protein human Homo sapiens Human MPARRLLLLLTLLLPGLGIFGSTSTVTLPETLLFVSTLDGSLHAVSKRTGSIKWTLKEDPVLQVPTHVEEPAFLPDPNDGSLYTLGSKNNEGLTKLPFTIPELVQASPCRSSDGILYMGKKQDIWYVIDLLTGEKQQTLSSAFADSLCPSTSLLYLGRTEYTITMYDTKTRELRWNATYFDYAASLPEDDVDYKMSHFVSNGDGLVVTVDSESGDVLWIQNYASPVVAFYVWQREGLRKVMHINVAVETLRYLTFMSGEVGRITKWKYPFPKETEAKSKLTPTLYVGKYSTSLYASPSMVHEGVAVVPRGSTLPLLEGPQTDGVTIGDKGECVITPSTDVKFDPGLKSKNKLNYLRNYWLLIGHHETPLSASTKMLERFPNNLPKHRENVIPADSEKKSFEEVINLVDQTSENAPTTVSRDVEEKPAHAPARPEAPVDSMLKDMATIILSTFLLIGWVAFIITYPLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL A2DF808CCE015536 bip_human Endoplasmic reticulum chaperone BiP HSPA5 GRP78 Immunoglobulin heavy chain-binding protein 78 kDa glucose-regulated protein Binding-immunoglobulin protein Heat shock protein 70 family protein 5 Heat shock protein family A member 5 protein protein human Homo sapiens Human MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL DIP protein P11021 original sequence version: 127 DIP protein P11021 original sequence version: 137 DIP protein P11021 original sequence version: 185 59B7D8D85BC32A00 tbb3_human Tubulin beta-3 chain TUBB3 TUBB4 Tubulin beta-III Tubulin beta-4 chain protein protein human Homo sapiens Human MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPSGNYVGDSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTARGSQQYRALTVPELTQQMFDAKNMMAACDPRHGRYLTVATVFRGRMSMKEVDEQMLAIQSKNSSYFVEWIPNNVKVAVCDIPPRGLKMSSTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEGEMYEDDEEESEAQGPK DIP protein Q13509 original sequence version: 137 4B9CDE7DBA102949 ldhb_human L-lactate dehydrogenase B chain LDHB LDH heart subunit Renal carcinoma antigen NY-REN-46 protein protein human Homo sapiens Human MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL 3AD605DEED0D54A2 rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR DIP protein P63244 original sequence version: 71 257F91E369ED2044 1433z_human 14-3-3 protein zeta/delta YWHAZ Protein kinase C inhibitor protein 1 protein protein human Homo sapiens Human MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN DIP protein P63104 original sequence version: 144 D464DF2286BBFE60 actg_human Actin, cytoplasmic 2 ACTG1 ACTG Gamma-actin protein protein human Homo sapiens Human MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF DIP protein P63261 original sequence version: null 54D08F986964EFD5 ef2_human Elongation factor 2 EEF2 EF2 protein protein human Homo sapiens Human MVNFTVDQIRAIMDKKANIRNMSVIAHVDHGKSTLTDSLVCKAGIIASARAGETRFTDTRKDEQERCITIKSTAISLFYELSENDLNFIKQSKDGAGFLINLIDSPGHVDFSSEVTAALRVTDGALVVVDCVSGVCVQTETVLRQAIAERIKPVLMMNKMDRALLELQLEPEELYQTFQRIVENVNVIISTYGEGESGPMGNIMIDPVLGTVGFGSGLHGWAFTLKQFAEMYVAKFAAKGEGQLGPAERAKKVEDMMKKLWGDRYFDPANGKFSKSATSPEGKKLPRTFCQLILDPIFKVFDAIMNFKKEETAKLIEKLDIKLDSEDKDKEGKPLLKAVMRRWLPAGDALLQMITIHLPSPVTAQKYRCELLYEGPPDDEAAMGIKSCDPKGPLMMYISKMVPTSDKGRFYAFGRVFSGLVSTGLKVRIMGPNYTPGKKEDLYLKPIQRTILMMGRYVEPIEDVPCGNIVGLVGVDQFLVKTGTITTFEHAHNMRVMKFSVSPVVRVAVEAKNPADLPKLVEGLKRLAKSDPMVQCIIEESGEHIIAGAGELHLEICLKDLEEDHACIPIKKSDPVVSYRETVSEESNVLCLSKSPNKHNRLYMKARPFPDGLAEDIDKGEVSARQELKQRARYLAEKYEWDVAEARKIWCFGPDGTGPNILTDITKGVQYLNEIKDSVVAGFQWATKEGALCEENMRGVRFDVHDVTLHADAIHRGGGQIIPTARRCLYASVLTAQPRLMEPIYLVEIQCPEQVVGGIYGVLNRKRGHVFEESQVAGTPMFVVKAYLPVNESFGFTADLRSNTGGQAFPQCVFDHWQILPGDPFDNSSRPSQVVAETRKRKGLKEGIPALDNFLDKL 78BD1710236C0D9C clh1_human Clathrin heavy chain 1 Clathrin heavy chain on chromosome 17 CLTC CLH17 KIAA0034 CLTCL2 protein protein human Homo sapiens Human MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGILRTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEKWLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKVGYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFLLDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQRALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQICVQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQIKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQKYIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLKLLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDPHLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLIDQVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLILTAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEHIGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANTSGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDRCYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVDGKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTELAILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITMMNHPTDAWKEGQFKDIITKVANVELYYRAIQFYLEFKPLLLNDLLMVLSPRLDHTRAVNYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNISLAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEELLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKVDKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQPGFGYSM 6C4F2D54950079E2 clic1_human Chloride intracellular channel protein 1 CLIC1 NCC27 Nuclear chloride ion channel 27 Chloride channel ABP Regulatory nuclear chloride ion channel protein G6 protein protein human Homo sapiens Human MAEEQPQVELFVKAGSDGAKIGNCPFSQRLFMVLWLKGVTFNVTTVDTKRRTETVQKLCPGGQLPFLLYGTEVHTDTNKIEEFLEAVLCPPRYPKLAALNPESNTAGLDIFAKFSAYIKNSNPALNDNLEKGLLKALKVLDNYLTSPLPEEVDETSAEDEGVSQRKFLDGNELTLADCNLLPKLHIVQVVCKKYRGFTIPEAFRGVHRYLSNAYAREEFASTCPDDEEIELAYEQVAKALK 163EEB7481826A0A tba1b_human Tubulin alpha-1B chain TUBA1B Tubulin alpha-ubiquitous chain Alpha-tubulin ubiquitous Tubulin K-alpha-1 protein protein human Homo sapiens Human MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY 94355B4EC2086429 tba1c_human Tubulin alpha-1C chain TUBA1C TUBA6 Tubulin alpha-6 chain Alpha-tubulin 6 protein protein human Homo sapiens Human MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAVAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGADSADGEDEGEEY 0CFE7212CB5E14F9 if4a1_human Eukaryotic initiation factor 4A-I EIF4A1 EIF4A DDX2A ATP-dependent RNA helicase eIF4A-1 protein protein human Homo sapiens Human MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI DIP protein P60842 original sequence version: 125 DIP protein P60842 original sequence version: 59 DIP protein P60842 original sequence version: 63 DIP protein P60842 original sequence version: 85 DIP protein P60842 original sequence version: 86 DIP protein P60842 original sequence version: 92 DIP protein P60842-1 original sequence version: 114 DIP protein P60842 original sequence version: 106 6EF89939F3045420 pgam1_human Phosphoglycerate mutase 1 Phosphoglycerate mutase isozyme B BPG-dependent PGAM 1 PGAM1 CDABP0006 PGAMA protein protein human Homo sapiens Human MAAYKLVLIRHGESAWNLENRFSGWYDADLSPAGHEEAKRGGQALRDAGYEFDICFTSVQKRAIRTLWTVLDAIDQMWLPVVRTWRLNERHYGGLTGLNKAETAAKHGEAQVKIWRRSYDVPPPPMEPDHPFYSNISKDRRYADLTEDQLPSCESLKDTIARALPFWNEEIVPQIKEGKRVLIAAHGNSLRGIVKHLEGLSEEAIMELNLPTGIPIVYELDKNLKPIKPMQFLGDEETVRKAMEAVAAQGKAKK 6DC0852BEBB22409 aldoa_human Fructose-bisphosphate aldolase A Muscle-type aldolase Lung cancer antigen NY-LU-1 ALDOA ALDA protein protein human Homo sapiens Human MPYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY DIP protein P04075 original sequence version: null 0AAED80F755A7BE8 enoa_human Alpha-enolase ENO1 ENO1L1 MBPB1 MPB1 2-phospho-D-glycerate hydro-lyase Non-neural enolase Enolase 1 Phosphopyruvate hydratase C-myc promoter-binding protein MBP-1 MPB-1 Plasminogen-binding protein protein protein human Homo sapiens Human MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK DIP protein P06733 original sequence version: null A0ED663FCC15ADA5 actn4_human Alpha-actinin-4 ACTN4 Non-muscle alpha-actinin 4 protein protein human Homo sapiens Human MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL DIP protein O43707 original sequence version: 184 DIP protein O43707 original sequence version: 139 461580C3F22937D1 ha15 HA15 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ha15-hspa5-8 1 16 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 32 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 3B ha15-hspa5-1 1 16 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 32 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3C ha15-hspa5-2 8 16 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association 3D HA15 (CHEBI:132781) eif2ak3-hspa5-1 8 16 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association 3D-2 HA15 (CHEBI:132781) ern1-hspa5-3 8 13 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait physical association physical association 3D-3 HA15 (CHEBI:132781) ha15-hspa5-3 2 32 unspecified role unspecified role neutral component neutral component region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role neutral component neutral component colocalization colocalization 3E ha15-hspa5-5 3 19 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 31 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 29 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 25 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 26 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 21 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 16 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 32 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 18 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 9 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 14 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 24 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 20 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 27 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 30 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 23 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 17 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 28 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 12 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 22 unspecified role unspecified role prey prey human-a375 Human malignant melanoma a375 Human malignant melanoma ATCC catalog # CRL-1872 association association Table 1 ha15-hspa5-4 1 16 unspecified role unspecified role prey prey human-melanoma Human melanoma melanoma Melanoma 32 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp 2B eif2ak3-hspa5-2 5 16 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Supp 2 C/D HA15 (CHEBI:132781) hspa5-atf6-1 5 13 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association Supp 2 C HA15 (CHEBI:132781) ern1-hspa5-4 5 16 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Supp 2 C/D HA15 (CHEBI:132781) 24w5t-1 4 32 predetermined predetermined participant predetermined tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role fluorescence donor fluorescence donor 16 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined proximity proximity close-contact colocalization neighbourhood interaction Supp 3A ha15-hspa5-6 7 32 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range nbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp 3B ha15-hspa5-7 6 32 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range nbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp 3C ern1-hspa5-5 5 16 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 16 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait physical association physical association Supp 2 C/Di HA15 (CHEBI:132781) It is stated that the addition of recombinant BiP competes and diminishes dissociation of endogenous cellular BiP from PERK and IRE1-alpha. eif2ak3-hspa5-3 5 16 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 16 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Supp 2 C/Dii HA15 (CHEBI:132781) It is stated that the addition of recombinant BiP competes and diminishes dissociation of endogenous cellular BiP from PERK and IRE1-alpha.