IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. Cancer cell 2016 imex curation Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer Dark Space no 15 HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-neuronal_precursor_cells(stem cells) Mouse Neuronal precursor cells/neural stem progenitor cells neuronal_precursor (stem cells) Neuronal Precursor Cells/neural stem progenitor cells ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Accepted 2016-MAR-03 AT 15:12 GMT AT 15:12 GMT by ORCHARD Cancer cell 2016 imex curation I have altered these. Figure 4F/Experiment -3 deleted (co-loc) as I dont think it should have been added, in retrospect. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. Cancer cell 2016 imex curation Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer Dark Space no 15 HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Accepted 2016-MAR-03 AT 15:12 GMT AT 15:12 GMT by ORCHARD Cancer cell 2016 imex curation I have altered these. Figure 4F/Experiment -3 deleted (co-loc) as I dont think it should have been added, in retrospect. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. Cancer cell 2016 imex curation Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer Dark Space no 15 HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-medulloblastoma Mouse medulloblastoma medulloblastoma Medulloblastoma ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer The authors confirm that Supplementary Figure 3F results were obtained from G3-MB cells. G3-MB cells; Group3 is a subgroup of (paediatric) medulloblastoma, here induced by c-Myc (Myc) overexpression in granule neuron progenitor cells (GNPs). (In humans Group 3 tumours are often associated with Myc ampification. They are associated with the worst prognosis of all the subgroups.) Granule neuron progenitors (GNPs) were infected with mouse stem cell virus (MSCV)-based retroviruses; these viruses either express RFP/GFP alone or co-express mouse Myc or Mycn cDNA. Infected GNP-enriched cerebellar cells were transplanted into the cortices or cerebellum of nude recipient mice. (Kawauchi et al.,) Extracted tumour cells were also grown in vitro as tumorspheres. HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Accepted 2016-MAR-03 AT 15:12 GMT AT 15:12 GMT by ORCHARD Cancer cell 2016 imex curation I have altered these. Figure 4F/Experiment -3 deleted (co-loc) as I dont think it should have been added, in retrospect. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. Cancer cell 2016 imex curation Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer Dark Space no 15 HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Accepted 2016-MAR-03 AT 15:12 GMT AT 15:12 GMT by ORCHARD Cancer cell 2016 imex curation I have altered these. Figure 4F/Experiment -3 deleted (co-loc) as I dont think it should have been added, in retrospect. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. Cancer cell 2016 imex curation Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer Dark Space no 15 HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-medulloblastoma Mouse medulloblastoma medulloblastoma Medulloblastoma ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer Sonic Hedgehog (SHH) medulloblastoma; here induced by MycN overexpression in granule neuron progenitor cells (GNPs). Granule neuron progenitors (GNPs) were infected with mouse stem cell virus (MSCV)-based retroviruses; these viruses either express RFP/GFP alone or co-express mouse Myc or Mycn cDNA. Infected GNP-enriched cerebellar cells were transplanted into the cortices or cerebellum of nude recipient mice. (Kawauchi et al.,) Extracted tumour cells were also grown in vitro as tumorspheres. HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Accepted 2016-MAR-03 AT 15:12 GMT AT 15:12 GMT by ORCHARD Cancer cell 2016 imex curation I have altered these. Figure 4F/Experiment -3 deleted (co-loc) as I dont think it should have been added, in retrospect. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. Cancer cell 2016 imex curation Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer Dark Space no 15 HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Accepted 2016-MAR-03 AT 15:12 GMT AT 15:12 GMT by ORCHARD Cancer cell 2016 imex curation I have altered these. Figure 4F/Experiment -3 deleted (co-loc) as I dont think it should have been added, in retrospect. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. The Interaction of Myc with Miz1 Defines Medulloblastoma Subgroup Identity. Cancer cell 2016 imex curation Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer Dark Space no 15 HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Vo BT., Wolf E., Kawauchi D., Gebhardt A., Rehg JE., Finkelstein D., Walz S., Murphy BL., Youn YH., Han YG., Eilers M., Roussel MF. Cancer - Interactions investigated in the context of cancer HA tagged Myc/Myc-N and mutants, Transfected Miz1 and mutant (delta POZ), and HA-MycNVD are stated by the authors to be of Human origin. The authors also state that the mutated Valine is V421 in MycN, as this residue is homologous to Valine 394 in c-Myc. Similarly, as used in a Pull-Down using Glutathione beads, "recombinant Miz1 protein" (E.coli expressed and GST tagged)" and "in-vitro translated, S-35 radiolabelled, Myc and MycN" are stated to be of Human origin. martin.eilers@biozentrum.uni-wuerzburg.de,martine.roussel@stjude.org Accepted 2016-MAR-03 AT 15:12 GMT AT 15:12 GMT by ORCHARD Cancer cell 2016 imex curation I have altered these. Figure 4F/Experiment -3 deleted (co-loc) as I dont think it should have been added, in retrospect. egr1_mouse_gene Mouse EGR1 gene gene gene mouse Mus musculus Mouse dcor_mouse_gene Ornithine decarboxylase Odc1 Odc gene gene mouse Mus musculus Mouse myc_mouse Myc proto-oncogene protein Proto-oncogene c-Myc Transcription factor p64 Myc protein protein mouse Mus musculus Mouse MPLNVNFTNRNYDLDYDSVQPYFICDEEENFYHQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGGGGNFSTADQLEMMTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSTSLSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKSCTSSDSTAFSPSSDSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQTPAKRSESGSSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAKLDSGRVLKQISNNRKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA DIP protein P01108 original sequence version: 146 DIP protein P01108 original sequence version: 167 3FCA39BFFD6FC59E zbt17_human Zinc finger and BTB domain-containing protein 17 ZBTB17 MIZ1 Zinc finger protein 60 Zinc finger protein 151 Myc-interacting zinc finger protein 1 ZNF151 ZNF60 protein protein human Homo sapiens Human MDFPQHSQHVLEQLNQQRQLGLLCDCTFVVDGVHFKAHKAVLAACSEYFKMLFVDQKDVVHLDISNAAGLGQVLEFMYTAKLSLSPENVDDVLAVATFLQMQDIITACHALKSLAEPATSPGGNAEALATEGGDKRAKEEKVATSTLSRLEQAGRSTPIGPSRDLKEERGGQAQSAASGAEQTEKADAPREPPPVELKPDPTSGMAAAEAEAALSESSEQEMEVEPARKGEEEQKEQEEQEEEGAGPAEVKEEGSQLENGEAPEENENEESAGTDSGQELGSEARGLRSGTYGDRTESKAYGSVIHKCEDCGKEFTHTGNFKRHIRIHTGEKPFSCRECSKAFSDPAACKAHEKTHSPLKPYGCEECGKSYRLISLLNLHKKRHSGEARYRCEDCGKLFTTSGNLKRHQLVHSGEKPYQCDYCGRSFSDPTSKMRHLETHDTDKEHKCPHCDKKFNQVGNLKAHLKIHIADGPLKCRECGKQFTTSGNLKRHLRIHSGEKPYVCIHCQRQFADPGALQRHVRIHTGEKPCQCVMCGKAFTQASSLIAHVRQHTGEKPYVCERCGKRFVQSSQLANHIRHHDNIRPHKCSVCSKAFVNVGDLSKHIIIHTGEKPYLCDKCGRGFNRVDNLRSHVKTVHQGKAGIKILEPEEGSEVSVVTVDDMVTLATEALAATAVTQLTVVPVGAAVTADETEVLKAEISKAVKQVQEEDPNTHILYACDSCGDKFLDANSLAQHVRIHTAQALVMFQTDADFYQQYGPGGTWPAGQVLQAGELVFRPRDGAEGQPALAETSPTAPECPPPAE DIP protein Q13105 original sequence version: 110 C159D177C8A2D4A3 tmm11_mouse_gene Transmembrane protein 11, mitochondrial Tmem11 gene gene mouse Mus musculus Mouse myc_human Myc proto-oncogene protein BHLHE39 Transcription factor p64 MYC Proto-oncogene c-Myc Class E basic helix-loop-helix protein 39 protein protein human Homo sapiens Human MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA DIP protein P01106 original sequence version: 195 DIP protein P01106 original sequence version: 196 DIP protein P01106 original sequence version: 148 DIP protein P01106-1 original sequence version: 161 DIP protein P01106 original sequence version: 182 DIP protein P01106 original sequence version: 197 DIP protein P01106-1 original sequence version: 208 DIP protein P01106 original sequence version: 121 DIP protein P01106-1 original sequence version: 144 DIP protein P01106 original sequence version: 144 ED5C028029A4C5D1 mycn_human N-myc proto-oncogene protein MYCN NMYC BHLHE37 Class E basic helix-loop-helix protein 37 protein protein human Homo sapiens Human MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC DIP protein P04198 original sequence version: 157 560E885602E30DAD pcbp1_mouse_gene Poly(rC)-binding protein 1 Pcbp1 gene gene mouse Mus musculus Mouse mouse_ccnd1_gene Ccnd1/G1/S-specific cyclin-D1 Mouse Gene gene gene mouse Mus musculus Mouse foxg1_mouse_gene Forkhead box protein G1 Foxg1 Fkhl1 Foxg1b Hfhbf1 gene gene mouse Mus musculus Mouse vps72_mouse_gene Vacuolar protein sorting-associated protein 72 homolog Vps72 Tcfl1 Yl1 gene gene mouse Mus musculus Mouse noct_mouse_gene Nocturnin Noct Ccr4 Ccrn4l Noc gene gene mouse Mus musculus Mouse zbt17_mouse Zinc finger and BTB domain-containing protein 17 Zbtb17 Zfp100 Znf151 LP-1 Polyomavirus late initiator promoter-binding protein Zinc finger protein 100 Zinc finger protein 151 Zinc finger protein Z13 protein protein mouse Mus musculus Mouse MDFPQHSQRVLEQLNQQRQLGLLCDCTFVVDGVDFKAHKAVLAACSEYFKMLFVDQKDVVHLDISNAAGLGQVLEFMYTAKLSLSPENVDDVLAVASFLQMQDIVTACHTLKSLAEPSSTTGESADASAVEGGDKRAKDEKAAATMLSRLDQARGSSSTGPGRELKEERGGQAESASSGAEQTEKADAPREPPPVELKPDPTSSMAAAEAEALSESSEQEMEVEPASKGEDGQEEEGAGPATVKEEGMHLDNGEPPEENEESAGTDSGQELGMEGQNLRSGTYGDRTESKAYGSIIHKCEDCGKEFTHTGNFKRHIRIHTGEKPFSCRECSKAFSDPAACKAHEKTHSPLKPYGCEECGKSYRLISLLNLHKKRHSGEARYRCGDCGKLFTTSGNLKRHQLVHSGQKPYQCDYCGRSFSDPTSKMRHLETHDTDKEHKCPHCDKKFNQVGNLKAHLKIHIADGPLKCRECGKQFTTSGNLKRHLRIHSGEKPYVCTHCQRQFADPGALQRHVRIHTGEKPCQCVICGKAFTQASSLIAHVRQHTGEKPYVCERCGKRFVQSSQLANHIRHHDNIRPHKCSVCSKAFVNVGDLSKHIIIHTGEKPYLCDKCGRGFNRVDNLRSHVKTVHQGKAGIKILEPEEGGEVSVVTVDDMVTLATEALAATAVTQLTVVPVGAAVTADETEVLKAEISKAVKQVQEEDPNTHILYACDSCGDKFLDANSLAQHVRIHTAQALVMFQTDADFYQQYGPGSTWPAGQMLQAGELVFRPRDGTEGQPTLAESPPTAPDCLPPAE DIP protein Q60821 original sequence version: 89 54CF6CF0D2C60303 atoh1_mouse_gene Protein atonal homolog 1 gene gene mouse Mus musculus Mouse ck2n1_mouse_gene Calcium/calmodulin-dependent protein kinase II inhibitor 1 Camk2n1 gene gene mouse Mus musculus Mouse myc-noct-1 3 10 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3B zbtb17-noct-2 3 20 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3B-2 zbtb17-noct-1 1 20 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3B-2 myc-atoh1_mouse_gene-1 3 10 unspecified role unspecified role bait bait 21 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 4E zbtb17-atoh1_mouse_gene-1 3 20 unspecified role unspecified role bait bait 21 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 4E-2 23541-1 6 11 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5D, Supp 3E/5E The Miz1_delta POZ protein still binds to Myc. mycn-zbtb17-2 6 11 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P04198:p.Val421Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V D Wed May 02 15:14:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val421asp' Tue Jun 30 17:28:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val421Asp' physical association physical association 5Di/Supp 5A/5D mycn-zbtb17-1 7 11 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5E zbtb17-myc-2 7 11 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5E zbtb17-egr1_mouse_gene-1 3 20 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5F-1 zbtb17-foxg1-1 3 20 unspecified role unspecified role bait bait 17 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5F-2 zbtb17-vps72-1 3 20 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain 18 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5F-3, Supp 3C/F zbtb17-vps72-2 5 20 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 5F-4 myc-vps72-1 3 10 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp 3C myc-odc1-1 3 10 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp 3Ci myc-odc1-2 3 9 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role bait bait association association Supp 3Cii myc-camk2n1-1 3 10 unspecified role unspecified role bait bait 22 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp 3Ciii zbtb17-camk2n1-1 3 20 unspecified role unspecified role bait bait 22 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp 3Civ zbtb17-myc-3 2 11 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey P01106:p.Val394Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V D Wed May 02 17:22:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val394asp' Tue Jun 30 18:35:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val394Asp' physical association physical association Supp 3D myc-zbtb17-4 2 13 unspecified role unspecified role bait bait P01106:p.Val394Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V D Wed May 02 19:31:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val394asp' Tue Jun 30 16:03:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val394Asp' 11 unspecified role unspecified role prey prey physical association physical association Supp 3Di zbtb17-vps72-3 3 20 unspecified role unspecified role bait bait poz_domain binding region binding-associated region binding region range range range range 15 unspecified role unspecified role prey prey association association Supp 3F zbtb17-mouse_ccnd1_gene-1 3 20 unspecified role unspecified role bait bait poz_domain binding region binding-associated region binding region range range range range 16 unspecified role unspecified role prey prey association association Supp 3F-1 zbtb17-mouse_ccnd1_gene-2 3 20 unspecified role unspecified role bait bait poz_domain binding region binding-associated region binding region range range range range 12 unspecified role unspecified role prey prey association association Supp 3F-2 zbtb17-myc-4 4 13 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp 5B zbtb17-mycn-1 4 14 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp 5Bi Very weak interaction.