IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. PLoS genetics 2015 imex curation Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J., Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J. wujr@sibs.ac.cn 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J., Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J. wujr@sibs.ac.cn Accepted 2016-APR-08 AT 09:48 BST AT 09:48 BST by MGT PLoS genetics 2015 Only protein-protein interactions imex curation imex curation CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. PLoS genetics 2015 imex curation Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J., Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J. wujr@sibs.ac.cn 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J., Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J. wujr@sibs.ac.cn Accepted 2016-APR-08 AT 09:48 BST AT 09:48 BST by MGT PLoS genetics 2015 Only protein-protein interactions imex curation imex curation CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. PLoS genetics 2015 imex curation Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J., Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J. wujr@sibs.ac.cn 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-testis Mus musculus testis testis confocal microscopy confocal microscopy immunohistochemistry immunohistochemistry Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J., Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J. wujr@sibs.ac.cn Accepted 2016-APR-08 AT 09:48 BST AT 09:48 BST by MGT PLoS genetics 2015 Only protein-protein interactions imex curation imex curation CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. PLoS genetics 2015 imex curation Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J., Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J. wujr@sibs.ac.cn 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J., Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J. wujr@sibs.ac.cn Accepted 2016-APR-08 AT 09:48 BST AT 09:48 BST by MGT PLoS genetics 2015 Only protein-protein interactions imex curation imex curation CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. CCNYL1, but Not CCNY, Cooperates with CDK16 to Regulate Spermatogenesis in Mouse. PLoS genetics 2015 imex curation Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J., Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J. wujr@sibs.ac.cn 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-testis Mus musculus testis testis anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J., Zi Z., Zhang Z., Li Q., An W., Zeng L., Gao D., Yang Y., Zhu X., Zeng R., Shum WW., Wu J. wujr@sibs.ac.cn Accepted 2016-APR-08 AT 09:48 BST AT 09:48 BST by MGT PLoS genetics 2015 Only protein-protein interactions imex curation imex curation d3yuj3_mouse Ccnyl1 protein protein mouse Mus musculus Mouse METRIRAAQLKGRGGAFPKLGRRAGPAEPDYESEVYEAAAGDAVAVAPAPAAAVEPAELDFGAGEGHHLQHISDREMPEDLALESNPSDHPRASTIFLSKSQTDVREKRKSNHLNHVSPGQLTKKYSSCSTIFLDDSTVSQPNLRTTIKCVTLAIYYHIKNRDANRSLDIFDERSHPLTREKVPEEYFKHDPEHKFIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPTNWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNEMERHFLELLQFNINVPASVYAKYYFDLRSLADDNNLNFLFAPLSKERAQNLEAISRLCEDKYKDLCRAAMRRSLSADNFIGIQRSNAILS C161CD6B38BEE3F8 cdk16_mouse Cyclin-dependent kinase 16 CRK5 Cell division protein kinase 16 PCTAIRE-motif protein kinase 1 Serine/threonine-protein kinase PCTAIRE-1 Cdk16 Crk5 Pctaire1 Pctk1 protein protein mouse Mus musculus Mouse MDRMKKIKRQLSMTLRGGRGIDKTNGVPEQIGLDESGGGGGSDLGEAPTRIAPGELRSVRGPLSSAPEIVHEDMKMGSDGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPEGYLEKLTLNSPIFDKPLSRRLRRVSLSEIGFGKLETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNVINMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFRTYNYPKYRAEALLSHAPRLDSDGADLLTKLLQFEGRNRISAEDARKHPFFLSLGERIHKLPDTTSIFALKEVQLQKEANIRSTSMPDSGRPAFRVVDTEF 0746036524B1FB5F ccny_mouse Cyclin-Y Cyclin fold protein 1 Ccny Cfp1 protein protein mouse Mus musculus Mouse MGNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENIDDLNMEFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQTSRKYSSCSTIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRERAHKLEAISRLCEDKYKDLRKPMRKRSASADNLILPRWSPAIIS 6F16CE5B14559FCB cdk16-1 3 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 5A ccnyl1-cdk16-1 5 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig. 5C cdk16-ccnyl1-4 2 7 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5D ccnyl1-cdk16-2 2 7 unspecified role unspecified role prey prey c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5D cdk16-ccnyl1-5 4 7 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5E, 5F Deletion of either the N terminal domain, the cyclin domain, or the C-terminal domain of CCNYL1 abrogated or greatly impaired the interaction cdk16-ccny-7 4 7 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role prey prey n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5E Weak interaction cdk16-ccnyl1-6 4 7 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range pctaire region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q04735:p.Ser78Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:09:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser78ala' Tue Jun 30 18:26:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser78Ala' Q04735:p.Thr175Asp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain T D Wed May 02 16:34:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr175asp' Tue Jun 30 18:50:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr175Asp' Q04735:p.Thr175Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Wed May 02 18:47:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr175ala' Tue Jun 30 15:47:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr175Ala' Q04735:p.Ser146Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:42:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser146ala' Tue Jun 30 18:13:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser146Ala' Q04735:p.Ser146Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 15:50:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser146asp' Tue Jun 30 17:09:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser146Asp' Q04735:p.Ser110Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:37:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser110ala' Tue Jun 30 18:37:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser110Ala' Q04735:p.Ser110Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 20:32:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser110asp' Tue Jun 30 20:21:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser110Asp' Q04735:p.Ser119Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:37:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser119ala' Tue Jun 30 20:03:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser119Ala' Q04735:p.Ser153Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:35:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser153ala' Tue Jun 30 17:29:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser153Ala' Q04735:p.Ser153Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S D Wed May 02 20:08:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser153asp' Tue Jun 30 16:49:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser153Asp' Q04735:p.[Pro205Ala;Thr207Ala;Arg210_Glu211delinsAlaAla] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain P A certain certain sequence position certain certain certain sequence position certain RE AA Fri Jul 22 10:50:46 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'pro205ala,thr207ala,arg210ala,glu211ala' Wed May 02 20:01:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro205ala,thr207ala,arg_glu210-211ala_ala' Tue Jun 30 18:13:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Pro205Ala;Thr207Ala;Arg210_Glu211delinsAlaAla]' 6 unspecified role unspecified role prey prey n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 5G, 7D, 7E, Supp fig 7. Both the N-terminal and C-terminal of CDK-16 were essential for CCNYL1 binding. ccnyl1-cdk16-3 1 7 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role neutral component neutral component n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization Supp Fig. 6