HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Identification of Mycoplasma suis MSG1 interaction proteins on porcine erythrocytes. Identification of Mycoplasma suis MSG1 interaction proteins on porcine erythrocytes. Archives of microbiology 2015 imex curation Zhang Y., Zou Y., Ma P., Muhammad HM., Li Y., Jiang P. yufengli@njau.edu.cn 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom ms participant Identification by mass spectrometry ms participant Zhang Y., Zou Y., Ma P., Muhammad HM., Li Y., Jiang P. yufengli@njau.edu.cn Accepted 2017-JAN-16 AT 13:39 GMT AT 13:39 GMT by MGT Archives of microbiology 2015 imex curation Identification of Mycoplasma suis MSG1 interaction proteins on porcine erythrocytes. Identification of Mycoplasma suis MSG1 interaction proteins on porcine erythrocytes. Archives of microbiology 2015 imex curation Zhang Y., Zou Y., Ma P., Muhammad HM., Li Y., Jiang P. yufengli@njau.edu.cn 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Zhang Y., Zou Y., Ma P., Muhammad HM., Li Y., Jiang P. yufengli@njau.edu.cn Accepted 2017-JAN-16 AT 13:39 GMT AT 13:39 GMT by MGT Archives of microbiology 2015 imex curation Identification of Mycoplasma suis MSG1 interaction proteins on porcine erythrocytes. Identification of Mycoplasma suis MSG1 interaction proteins on porcine erythrocytes. Archives of microbiology 2015 imex curation Zhang Y., Zou Y., Ma P., Muhammad HM., Li Y., Jiang P. yufengli@njau.edu.cn 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation pig-erythrocyte pig erythrocyte Pig pig erythrocyte pig erythrocyte confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Zhang Y., Zou Y., Ma P., Muhammad HM., Li Y., Jiang P. yufengli@njau.edu.cn Accepted 2017-JAN-16 AT 13:39 GMT AT 13:39 GMT by MGT Archives of microbiology 2015 imex curation q05g10_9molu Glyceraldehyde-3-phosphate dehydrogenase g1 protein protein Mycoplasma suis Mycoplasma suis MTIHKVAINGFGRIGRLLFRNLLSSQGVQVVAVNDVVDIKVLTHLLVYDSAQGKLKDWEVSCDSEYIRLKNVNTGEVREVRVFNFNTEKIYHWGELEIDCVVECSGRFLTKEAVKCHLDAGAQKVLISAPAKDDTKTVVYNVNHTQITSSDNVISGASCTTNALAPIVKIIHRKFGINSGFMTTVHAFTSDQRLQDSPHSDLRRARAAAGSIIPTTTGAAAAIGRVIPELNGKLDGIAHRVPVLTGSLVDLCLKINKSVSAEEINEAIKDGENETLAYVEDPIVSADIIGDTHGSVFDSSLTKVLPTGEVKLYAWYDNESSYVNQLARTLKYYISL E8DE644079AB10C2 f1rqy6_pig Anion exchange protein SLC4A1 protein protein pig Sus scrofa (Pig) Pig MDEKNQEMQWMERARWVRLEENLGKDGTWGRPHLSYLTFWNLLELQKAFTKGTVLLDLPEKSLPGVVVQLLDRFIFEGQIRPEHREPLLRTLLLKHSHARDIEVLGEMKPALLTPAGPSQPLLPQQPSLEVELFCEQGEGSMEGHSSEILGKSPENLDTLVLVGQVSFLERPVLGFVRLKEPMEMEQETEQEMEQKPEETEALAVPLRFLMVLLGPEDPHIDYNQLGRAAATLLSEKVFHAEARVAQSKEELVHSLGGFLDCSLVLPPSDTYSEKDLLSLIPVQRALLKRRKLPSPAKQEPSFYMGLDVDGGIEDKDDPLRRTGKLFGGLVRDIRRRYPRYLSDITDALSPQVLSAIIFIYFAALSPAITFGGLLGEKTQNLMGVSELLISTSAQGIVFSLLGAQPLLVVGFSGPLLVFEEAFYSFCQSNGLEYIVGRVWIGYWLIFLVVLVVAFEGSFLVRFISRYTQEIFSFLISLIFIFETFKKLYKIFEEHPLKKDYSAAGPSQPNTALLSLVLMAGTFFLAMLLRKFKNSSYFPGKLRRVIGDFGVPISILIMVLVDVFIEETYTQKLSVPSGFTVSNSSARGWLIHPLGQGGSFPIWMMFASVLPAMLVFILIFLETQITTLIVSKPERKMVKGSGFHLDLLLIMGMGGVAAIFGLPWLSATTVRSVTHANALTVMSKSSIPGAASQIQEVKEQRISGLLVAVLVGLSILMGPILSHIPLAVLFGIFLYMGVTSLSGIQLFDRILLLFKPSKYHPDIPYAKRVRTWRMHLYTLTQIICLVVLWTVKFFPSTSLALPFVLILTVPLRLFLLPLIFRKLELQCLDADDAKPNLDEEHGRDEYDEVHMPV The sequence has been withdrawn from uniprot. 14CE2152551DEE1C actb_pig Actin, cytoplasmic 1 Beta-actin ACTB protein protein pig Sus scrofa (Pig) Pig MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 6AFD05CA94E360E2 g1-actb-1 1 4 unspecified role unspecified role bait bait his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey pig-erythrocyte pig erythrocyte Pig pig erythrocyte pig erythrocyte 5 unspecified role unspecified role prey prey pig-erythrocyte pig erythrocyte Pig pig erythrocyte pig erythrocyte physical association physical association 1 g1-actb-2 3 4 unspecified role unspecified role neutral component neutral component his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 4A actb-g1-1 3 4 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 4B actb-12 2 4 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 5