HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry. Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry. The Journal of biological chemistry 2014 imex curation Snyder GA., Deredge D., Waldhuber A., Fresquez T., Wilkins DZ., Smith PT., Durr S., Cirl C., Jiang J., Jennings W., Luchetti T., Snyder N., Sundberg EJ., Wintrode P., Miethke T., Xiao TS. Gsnyder@som.umaryland.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Snyder GA., Deredge D., Waldhuber A., Fresquez T., Wilkins DZ., Smith PT., Durr S., Cirl C., Jiang J., Jennings W., Luchetti T., Snyder N., Sundberg EJ., Wintrode P., Miethke T., Xiao TS. Gsnyder@som.umaryland.edu Accepted 2017-NOV-28 AT 10:14 GMT AT 10:14 GMT by MGT The Journal of biological chemistry 2014 imex curation Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry. Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry. The Journal of biological chemistry 2014 imex curation Snyder GA., Deredge D., Waldhuber A., Fresquez T., Wilkins DZ., Smith PT., Durr S., Cirl C., Jiang J., Jennings W., Luchetti T., Snyder N., Sundberg EJ., Wintrode P., Miethke T., Xiao TS. Gsnyder@som.umaryland.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro h1-h2 ms mass spectrometry study of hydrogen/deuterium exchange h1-h2 ms predetermined predetermined participant predetermined Snyder GA., Deredge D., Waldhuber A., Fresquez T., Wilkins DZ., Smith PT., Durr S., Cirl C., Jiang J., Jennings W., Luchetti T., Snyder N., Sundberg EJ., Wintrode P., Miethke T., Xiao TS. Gsnyder@som.umaryland.edu Accepted 2017-NOV-28 AT 10:14 GMT AT 10:14 GMT by MGT The Journal of biological chemistry 2014 imex curation Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry. Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry. The Journal of biological chemistry 2014 imex curation Snyder GA., Deredge D., Waldhuber A., Fresquez T., Wilkins DZ., Smith PT., Durr S., Cirl C., Jiang J., Jennings W., Luchetti T., Snyder N., Sundberg EJ., Wintrode P., Miethke T., Xiao TS. Gsnyder@som.umaryland.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Snyder GA., Deredge D., Waldhuber A., Fresquez T., Wilkins DZ., Smith PT., Durr S., Cirl C., Jiang J., Jennings W., Luchetti T., Snyder N., Sundberg EJ., Wintrode P., Miethke T., Xiao TS. Gsnyder@som.umaryland.edu Accepted 2017-NOV-28 AT 10:14 GMT AT 10:14 GMT by MGT The Journal of biological chemistry 2014 imex curation Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry. Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry. The Journal of biological chemistry 2014 imex curation Snyder GA., Deredge D., Waldhuber A., Fresquez T., Wilkins DZ., Smith PT., Durr S., Cirl C., Jiang J., Jennings W., Luchetti T., Snyder N., Sundberg EJ., Wintrode P., Miethke T., Xiao TS. Gsnyder@som.umaryland.edu 19-Dec-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Snyder GA., Deredge D., Waldhuber A., Fresquez T., Wilkins DZ., Smith PT., Durr S., Cirl C., Jiang J., Jennings W., Luchetti T., Snyder N., Sundberg EJ., Wintrode P., Miethke T., Xiao TS. Gsnyder@som.umaryland.edu Accepted 2017-NOV-28 AT 10:14 GMT AT 10:14 GMT by MGT The Journal of biological chemistry 2014 imex curation q8yf53_brume TIR domain-containing protein BMEI1674 protein protein brume Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) MSKEKQAQSKAHKAQQAISSAKSLSTQKSKMSELERATRDGAAIGKKRADIAKKIADKAKQLSSYQAKQFKADEQAVKKVAQEQKRLSDERTKHEAFIKQSLSSMRTTASATMEAEEEYDFFISHASEDKEAFVQDLVAALRDLGAKIFYDAYTLKVGDSLRRKIDQGLANSKFGIVVLSEHFFSKQWPARELDGLTAMEIGGQTRILPIWHKVSYDEVRRFSPSLADKVALNTSLKSVEEIAKELHSLI 8A3C0788BD26780F tirap_human Toll/interleukin-1 receptor domain-containing adapter protein MyD88 adapter-like protein Adaptor protein Wyatt TIRAP MAL protein protein human Homo sapiens Human MASSTSLPAPGSRPKKPLGKMADWFRQTLLKKPKKRPNSPESTSSDASQPTSQDSPLPPSLSSVTSPSLPPTHASDSGSSRWSKDYDVCVCHSEEDLVAAQDLVSYLEGSTASLRCFLQLRDATPGGAIVSELCQALSSSHCRVLLITPGFLQDPWCKYQMLQALTEAPGAEGCTIPLLSGLSRAAYPPELRFMYYVDGRGPDGGFRQVKEAVMRYLQTLS DIP protein P58753 original sequence version: 119 44A8454D04704540 tcpb-tirap-2 3 6 unspecified role unspecified role prey prey myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2 tcpb-tcpb-1 4 5 unspecified role unspecified role neutral component neutral component purified purified TIR domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8YF53:p.[Ser124Ile;Ser127Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S I Fri Jul 22 11:13:54 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser124ala_ser127ile' Wed May 02 15:42:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser124ile,ser127ala' Tue Jun 30 19:28:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser124Ile;Ser127Ala]' c terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction table 1 2.3 Å Rfree=0.263, Rworking=0.210 tirap-1 4 6 unspecified role unspecified role neutral component neutral component TIR domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction table 1 2.45 Å Rwork=0.207, RFree=0.218 tcpb-tcpb-2 1 5 unspecified role unspecified role unspecified role unspecified role c terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role unspecified role unspecified role c terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 5B Size exclusion chromatography of the full-length wild type and mutant TcpB samples revealed dimeric and oligomeric species. tcpb-tcpb-3 1 5 unspecified role unspecified role neutral component neutral component purified purified TIR_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8YF53:p.[Ser124Ile;Ser127Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S I Fri Jul 22 11:00:25 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser124ala_ser127ile' Wed May 02 16:35:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser124ile,ser127ala' Tue Jun 30 18:42:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser124Ile;Ser127Ala]' c terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified TIR_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8YF53:p.[Ser124Ile;Ser127Ala] mutation mutation certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S I Fri Jul 22 10:56:22 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser124ala_ser127ile' Wed May 02 19:29:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser124ile,ser127ala' Tue Jun 30 20:15:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser124Ile;Ser127Ala]' c terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 5B The chymotrypsin-treated TcpBS124A/S127I yielded a TIR domain fragment that eluted at a volume consistent with a primarily monomeric state. tcpb-tcpb-5 2 5 unspecified role unspecified role neutral component neutral component purified purified c terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified c terminus strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 5C and 6