IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Inter-α-inhibitor impairs TSG-6-induced hyaluronan cross-linking. Inter-α-inhibitor impairs TSG-6-induced hyaluronan cross-linking. The Journal of biological chemistry 2013 imex curation Baranova NS., Foulcer SJ., Briggs DC., Tilakaratna V., Enghild JJ., Milner CM., Day AJ., Richter RP. It has not been possible to annotate interactions involving Inter-alpha-inhibitor prepared from serum, as the exact composition of IAI cannot be determined from ref. 19. Dark space = 31? Prep of "Inter-α-inhibitor " from Human serum: I cannot read details in Ref 19 (1989) describing the preparation of IAI - probably not possible to work out exact mols involved anyway, as there are many possible combinations. (maybe your eyesight is better than mine??!) rrichter@cicbiomagune.es,anthony.day@manchester.ac.uk 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Baranova NS., Foulcer SJ., Briggs DC., Tilakaratna V., Enghild JJ., Milner CM., Day AJ., Richter RP. It has not been possible to annotate interactions involving Inter-alpha-inhibitor prepared from serum, as the exact composition of IAI cannot be determined from ref. 19. Dark space = 31? Prep of "Inter-α-inhibitor " from Human serum: I cannot read details in Ref 19 (1989) describing the preparation of IAI - probably not possible to work out exact mols involved anyway, as there are many possible combinations. (maybe your eyesight is better than mine??!) Hyaluronic Acid films were formed on a streptavidin monolayer formed by exposure of streptavidin to a gold surface that had been functionalized with a biotinylated oligoethylene glycol monolayer. Binding of HA was detected by Ellipsometry;this measures changes in the polarization of light upon reflection at a planar surface. rrichter@cicbiomagune.es,anthony.day@manchester.ac.uk Accepted 2016-JUN-13 AT 09:17 BST AT 09:17 BST by ORCHARD The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation Inter-α-inhibitor impairs TSG-6-induced hyaluronan cross-linking. Inter-α-inhibitor impairs TSG-6-induced hyaluronan cross-linking. The Journal of biological chemistry 2013 imex curation Baranova NS., Foulcer SJ., Briggs DC., Tilakaratna V., Enghild JJ., Milner CM., Day AJ., Richter RP. It has not been possible to annotate interactions involving Inter-alpha-inhibitor prepared from serum, as the exact composition of IAI cannot be determined from ref. 19. Dark space = 31? Prep of "Inter-α-inhibitor " from Human serum: I cannot read details in Ref 19 (1989) describing the preparation of IAI - probably not possible to work out exact mols involved anyway, as there are many possible combinations. (maybe your eyesight is better than mine??!) rrichter@cicbiomagune.es,anthony.day@manchester.ac.uk 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Baranova NS., Foulcer SJ., Briggs DC., Tilakaratna V., Enghild JJ., Milner CM., Day AJ., Richter RP. It has not been possible to annotate interactions involving Inter-alpha-inhibitor prepared from serum, as the exact composition of IAI cannot be determined from ref. 19. Dark space = 31? Prep of "Inter-α-inhibitor " from Human serum: I cannot read details in Ref 19 (1989) describing the preparation of IAI - probably not possible to work out exact mols involved anyway, as there are many possible combinations. (maybe your eyesight is better than mine??!) rrichter@cicbiomagune.es,anthony.day@manchester.ac.uk Accepted 2016-JUN-13 AT 09:17 BST AT 09:17 BST by ORCHARD The Journal of biological chemistry 2013 Only protein-protein interactions imex curation imex curation hyaluronan Hyaluronan small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) q06033-pro_0000016530 Inter-alpha-trypsin inhibitor heavy chain H3 ITIH3 Serum-derived hyaluronan-associated protein protein protein human Homo sapiens Human LPEGVANGIEVYSTKINSKVTSRFAHNVVTMRAVNRADTAKEVSFDVELPKTAFITNFTLTIDGVTYPGNVKEKEVAKKQYEKAVSQGKTAGLVKASGRKLEKFTVSVNVAAGSKVTFELTYEELLKRHKGKYEMYLKVQPKQLVKHFEIEVDIFEPQGISMLDAEASFITNDLLGSALTKSFSGKKGHVSFKPSLDQQRSCPTCTDSLLNGDFTITYDVNRESPGNVQIVNGYFVHFFAPQGLPVVPKNVAFVIDISGSMAGRKLEQTKEALLRILEDMQEEDYLNFILFSGDVSTWKEHLVQATPENLQEARTFVKSMEDKGMTNINDGLLRGISMLNKAREEHRIPERSTSIVIMLTDGDANVGESRPEKIQENVRNAIGGKFPLYNLGFGNNLNYNFLENMALENHGFARRIYEDSDADLQLQGFYEEVANPLLTGVEMEYPENAILDLTQNTYQHFYDGSEIVVAGRLVDEDMNSFKADVKGHGATNDLTFTEEVDMKEMEKALQERDYIFGNYIERLWAYLTIEQLLEKRKNAHGEEKENLTARALDLSLKYHFVTPLTSMVVTKPEDNEDERAIADKPGEDAEATPVSPAMSYLTSYQPPQNPYYYVDGD 35 651 0A88739936D723C8 tsg6_human Tumor necrosis factor-inducible gene 6 protein TSG6 TNFAIP6 Hyaluronate-binding protein TNF-stimulated gene 6 protein Tumor necrosis factor alpha-induced protein 6 protein protein human Homo sapiens Human MIILIYLFLLLWEDTQGWGFKDGIFHNSIWLERAAGVYHREARSGKYKLTYAEAKAVCEFEGGHLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGVFTDPKQIFKSPGFPNEYEDNQICYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPDDIISTGNVMTLKFLSDASVTAGGFQIKYVAMDPVSKSSQGKNTSTTSTGNKNFLAGRFSHL E2B3AEB76353A782 p19823-pro_0000016518 Inter-alpha-trypsin inhibitor heavy chain H2 ITIH2 IGHEP2 Inter-alpha-trypsin inhibitor complex component II Serum-derived hyaluronan-associated protein protein protein human Homo sapiens Human SLPGESEEMMEEVDQVTLYSYKVQSTITSRMATTMIQSKVVNNSPQPQNVVFDVQIPKGAFISNFSMTVDGKTFRSSIKEKTVGRALYAQARAKGKTAGLVRSSALDMENFRTEVNVLPGAKVQFELHYQEVKWRKLGSYEHRIYLQPGRLAKHLEVDVWVIEPQGLRFLHVPDTFEGHFDGVPVISKGQQKAHVSFKPTVAQQRICPNCRETAVDGELVVLYDVKREEKAGELEVFNGYFVHFFAPDNLDPIPKNILFVIDVSGSMWGVKMKQTVEAMKTILDDLRAEDHFSVIDFNQNIRTWRNDLISATKTQVADAKRYIEKIQPSGGTNINEALLRAIFILNEANNLGLLDPNSVSLIILVSDGDPTVGELKLSKIQKNVKENIQDNISLFSLGMGFDVDYDFLKRLSNENHGIAQRIYGNQDTSSQLKKFYNQVSTPLLRNVQFNYPHTSVTDVTQNNFHNYFGGSEIVVAGKFDPAKLDQIESVITATSANTQLVLETLAQMDDLQDFLSKDKHADPDFTRKLWAYLTINQLLAERSLAPTAAAKRRITRSILQMSLDHHIVTPLTSLVIENEAGDERMLADAPPQDPSCCSGALYYGSKVVPDSTPSWANPSPTPVISMLAQGSQVLESTPPPHVMRVEND 55 702 8B1D2AEF5146B778 p19827-pro_0000016507 Inter-alpha-trypsin inhibitor heavy chain H1 ITIH1 IGHEP1 Inter-alpha-trypsin inhibitor complex component III Serum-derived hyaluronan-associated protein protein protein human Homo sapiens Human SKSSEKRQAVDTAVDGVFIRSLKVNCKVTSRFAHYVVTSQVVNTANEAREVAFDLEIPKTAFISDFAVTADGNAFIGDIKDKVTAWKQYRKAAISGENAGLVRASGRTMEQFTIHLTVNPQSKVTFQLTYEEVLKRNHMQYEIVIKVKPKQLVHHFEIDVDIFEPQGISKLDAQASFLPKELAAQTIKKSFSGKKGHVLFRPTVSQQQSCPTCSTSLLNGHFKVTYDVSRDKICDLLVANNHFAHFFAPQNLTNMNKNVVFVIDISGSMRGQKVKQTKEALLKILGDMQPGDYFDLVLFGTRVQSWKGSLVQASEANLQAAQDFVRGFSLDEATNLNGGLLRGIEILNQVQESLPELSNHASILIMLTDGDPTEGVTDRSQILKNVRNAIRGRFPLYNLGFGHNVDFNFLEVMSMENNGRAQRIYEDHDATQQLQGFYSQVAKPLLVDVDLQYPQDAVLALTQNHHKQYYEGSEIVVAGRIADNKQSSFKADVQAHGEGQEFSITCLVDEEEMKKLLRERGHMLENHVERLWAYLTIQELLAKRMKVDREERANLSSQALQMSLDYGFVTPLTSMSIRGMADQDGLKPTIDKPSEDSPPLEMLGPRRTFVLSALQPSPTHSSSNTQRLPDRVTGVDTD 35 672 0BEAE288471B9C4F hyaluronan-tnfaip6-1 1 5 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 3 unspecified role unspecified role bait bait N_acetyl_glucosamine_NH-link biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 1, 4 The addition of Inter-alpha-trypsin inhibitor (exact composition unknown) from human serum initially inhibited this reaction. itih1-tnfaip6-1 2 7 unspecified role unspecified role bait bait n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 2 itih1-tnfaip6-2 2 6 unspecified role unspecified role bait bait n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 2-2 itih2-tnfaip6-1 2 4 unspecified role unspecified role bait bait n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. direct interaction direct interaction 2-3