IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural basis of DNA ligase IV-Artemis interaction in nonhomologous end-joining. Structural basis of DNA ligase IV-Artemis interaction in nonhomologous end-joining. Cell reports 2012 imex curation De Ioannes P., Malu S., Cortes P., Aggarwal AK. Dark space = no 22? Patricia.Cortes@mssm.edu,Aneel.Aggarwal@mssm.edu 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined De Ioannes P., Malu S., Cortes P., Aggarwal AK. Dark space = no 22? Patricia.Cortes@mssm.edu,Aneel.Aggarwal@mssm.edu Accepted 2017-AUG-09 AT 15:15 BST AT 15:15 BST by PPORRAS Cell reports 2012 Only protein-protein interactions imex curation imex curation Structural basis of DNA ligase IV-Artemis interaction in nonhomologous end-joining. Structural basis of DNA ligase IV-Artemis interaction in nonhomologous end-joining. Cell reports 2012 imex curation De Ioannes P., Malu S., Cortes P., Aggarwal AK. Dark space = no 22? Patricia.Cortes@mssm.edu,Aneel.Aggarwal@mssm.edu 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined De Ioannes P., Malu S., Cortes P., Aggarwal AK. Dark space = no 22? Patricia.Cortes@mssm.edu,Aneel.Aggarwal@mssm.edu Accepted 2017-AUG-09 AT 15:15 BST AT 15:15 BST by PPORRAS Cell reports 2012 Only protein-protein interactions imex curation imex curation Structural basis of DNA ligase IV-Artemis interaction in nonhomologous end-joining. Structural basis of DNA ligase IV-Artemis interaction in nonhomologous end-joining. Cell reports 2012 imex curation De Ioannes P., Malu S., Cortes P., Aggarwal AK. Dark space = no 22? Patricia.Cortes@mssm.edu,Aneel.Aggarwal@mssm.edu 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined De Ioannes P., Malu S., Cortes P., Aggarwal AK. Dark space = no 22? Patricia.Cortes@mssm.edu,Aneel.Aggarwal@mssm.edu Accepted 2017-AUG-09 AT 15:15 BST AT 15:15 BST by PPORRAS Cell reports 2012 Only protein-protein interactions imex curation imex curation Structural basis of DNA ligase IV-Artemis interaction in nonhomologous end-joining. Structural basis of DNA ligase IV-Artemis interaction in nonhomologous end-joining. Cell reports 2012 imex curation De Ioannes P., Malu S., Cortes P., Aggarwal AK. Dark space = no 22? Patricia.Cortes@mssm.edu,Aneel.Aggarwal@mssm.edu 05-Sep-2017: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot De Ioannes P., Malu S., Cortes P., Aggarwal AK. Dark space = no 22? Patricia.Cortes@mssm.edu,Aneel.Aggarwal@mssm.edu Accepted 2017-AUG-09 AT 15:15 BST AT 15:15 BST by PPORRAS Cell reports 2012 Only protein-protein interactions imex curation imex curation dnli4_human DNA ligase 4 DNA ligase IV Polydeoxyribonucleotide synthase [ATP] 4 LIG4 protein protein human Homo sapiens Human MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAIADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNEAIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI DIP protein P49917 original sequence version: 127 DIP protein P49917 original sequence version: 151 DIP protein P49917 original sequence version: 144 2122813E1EFA63B9 xrcc4_human DNA repair protein XRCC4 X-ray repair cross-complementing protein 4 XRCC4 protein protein human Homo sapiens Human MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI DIP protein Q13426 original sequence version: 140 DIP protein Q13426-1 original sequence version: 116 BE5FB99153479A4E dcr1c_human Protein artemis DNA cross-link repair 1C protein Protein A-SCID SNM1 homolog C SNM1-like protein DCLRE1C ARTEMIS ASCID SCIDA SNM1C protein protein human Homo sapiens Human MSSFEGQMAEYPTISIDRFDRENLRARAYFLSHCHKDHMKGLRAPTLKRRLECSLKVYLYCSPVTKELLLTSPKYRFWKKRIISIEIETPTQISLVDEASGEKEEIVVTLLPAGHCPGSVMFLFQGNNGTVLYTGDFRLAQGEAARMELLHSGGRVKDIQSVYLDTTFCDPRFYQIPSREECLSGVLELVRSWITRSPYHVVWLNCKAAYGYEYLFTNLSEELGVQVHVNKLDMFRNMPEILHHLTTDRNTQIHACRHPKAEEYFQWSKLPCGITSRNRIPLHIISIKPSTMWFGERSRKTNVIVRTGESSYRACFSFHSSYSEIKDFLSYLCPVNAYPNVIPVGTTMDKVVEILKPLCRSSQSTEPKYKPLGKLKRARTVHRDSEEEDDYLFDDPLPIPLRHKVPYPETFHPEVFSMTAVSEKQPEKLRQTPGCCRAECMQSSRFTNFVDCEESNSESEEEVGIPASLQGDLGSVLHLQKADGDVPQWEVFFKRNDEITDESLENFPSSTVAGGSQSPKLFSDSDGESTHISSQNSSQSTHITEQGSQGWDSQSDTVLLSSQERNSGDITSLDKADYRPTIKENIPASLMEQNVICPKDTYSDLKSRDKDVTIVPSTGEPTTLSSETHIPEEKSLLNLSTNADSQSSSDFEVPSTPEAELPKREHLQYLYEKLATGESIAVKKRKCSLLDT 24B857F5B473637B 364wedtr-1 1 7 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role neutral component neutral component dbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 2, table S1 2.25A Rwork - 0.196 R free - 0.249 dclre1c-lig4-1 3 7 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role neutral component neutral component purified purified dbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P49917:p.Phe42Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:10:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe42ala' Tue Jun 30 19:58:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe42Ala' P49917:p.Asp18His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D H Wed May 02 19:01:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp18his' Tue Jun 30 17:23:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp18His' P49917:p.Phe49Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 19:12:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe49ala' Tue Jun 30 20:18:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe49Ala' P49917:p.Val14Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V A Wed May 02 15:31:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val14ala' Tue Jun 30 16:51:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val14Ala' P49917:p.[Phe42Ala;Phe49Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain F A Wed May 02 20:20:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe42ala,phe49ala' Tue Jun 30 18:31:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe42Ala;Phe49Ala]' ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 3 dclre1c-lig4-2 4 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 3H lig4-dclre1c-1 4 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait P49917:p.Phe42Ala mutation mutation certain certain sequence position certain certain certain sequence position certain F A Wed May 02 17:36:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe42ala' Tue Jun 30 16:57:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe42Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 3Hi Interaction with Artemis is much reduced. lig4-dclre1c-2 4 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait P49917:p.Phe49Ala mutation mutation certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:43:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe49ala' Tue Jun 30 18:45:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe49Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 3Hii dclre1c-lig4-4 2 7 unspecified role unspecified role neutral component neutral component purified purified region fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role neutral component neutral component purified purified dbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 4A