UniProt UniProt http://www.uniprot.org A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. The Journal of cell biology 2012 imex curation Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp Accepted 2016-OCT-06 AT 09:46 BST AT 09:46 BST by ORCHARD The Journal of cell biology 2012 imex curation A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. The Journal of cell biology 2012 imex curation Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-testis Mus musculus testis testis anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp Accepted 2016-OCT-06 AT 09:46 BST AT 09:46 BST by ORCHARD The Journal of cell biology 2012 imex curation A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. The Journal of cell biology 2012 imex curation Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp Accepted 2016-OCT-06 AT 09:46 BST AT 09:46 BST by ORCHARD mouse testis cDNA The Journal of cell biology 2012 imex curation A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. The Journal of cell biology 2012 imex curation Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp Accepted 2016-OCT-06 AT 09:46 BST AT 09:46 BST by ORCHARD The Journal of cell biology 2012 imex curation A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. The Journal of cell biology 2012 imex curation Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp 17-Oct-2016: Contacted by IntAct-Help. Only protein-protein interactions mouse-spermatocyte Mus musculus spermatocyte Spermatocyte fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Morimoto A., Shibuya H., Zhu X., Kim J., Ishiguro K., Han M., Watanabe Y. ywatanab@iam.u-tokyo.ac.jp Accepted 2016-OCT-06 AT 09:46 BST AT 09:46 BST by ORCHARD The Journal of cell biology 2012 imex curation sun2_mouse SUN domain-containing protein 2 Sad1/unc-84 protein-like 2 Sun2 Unc84b Protein unc-84 homolog B protein protein mouse Mus musculus Mouse MSRRSQRLTRYSQDDNDGGSSSSGASSVAGSQGTVFKDSPLRTLKRKSSNMKHLSPAPQLGPSSDSHTSYYSESVVRESYIGSPRAVSLARSALLDDHLHSEPYWSGDLRGRRRRGTGGSESSKANGLTAESKASEDFFGSSSGYSSEDDLAGYTDSDQHSSGSRLRSAASRAGSFVWTLVTFPGRLFGLLYWWIGTTWYRLTTAASLLDVFVLTRSRHFSLNLKSFLWFLLLLLLLTGLTYGAWHFYPLGLQTLQPAVVSWWAAKESRKQPEVWESRDASQHFQAEQRVLSRVHSLERRLEALAADFSSNWQKEAIRLERLELRQGAAGHGGGSSLSHEDALSLLEGLVSRREATLKEDLRRDTVAHIQEELATLRAEHHQDSEDLFKKIVQASQESEARVQQLKTEWKSMTQEAFQESSVKELGRLEAQLASLRQELAALTLKQNSVADEVGLLPQKIQAARADVESQFPDWIRQFLLGDRGARSGLLQRDEMHAQLQELENKILTKMAEMQGKSAREAAASLGQILQKEGIVGVTEEQVHRIVKQALQRYSEDRIGMVDYALESGGASVISTRCSETYETKTALLSLFGIPLWYHSQSPRVILQPDVHPGNCWAFQGPQGFAVVRLSARIRPTAVTLEHVPKALSPNSTISSAPKDFAIFGFDEDLQQEGTLLGTFAYDQDGEPIQTFYFQASKMATYQVVELRILTNWGHPEYTCIYRFRVHGEPAH 67830D40C33E3DBA sun1_mouse SUN domain-containing protein 1 Sun1 Unc84a Protein unc-84 homolog A Sad1/unc-84 protein-like 1 protein protein mouse Mus musculus Mouse MDFSRLHTYTPPQCVPENTGYTYALSSSYSSDALDFETEHKLEPVFDSPRMSRRSLRLVTTASYSSGDSQAIDSHISTSRATPAKGRETRTVKQRRSASKPAFSINHLSGKGLSSSTSHDSSCSLRSATVLRHPVLDESLIREQTKVDHFWGLDDDGDLKGGNKAATQGNGELAAEVASSNGYTCRDCRMLSARTDALTAHSAIHGTTSRVYSRDRTLKPRGVSFYLDRTLWLAKSTSSSFASFIVQLFQVVLMKLNFETYKLKGYESRAYESQSYETKSHESEAHLGHCGRMTAGELSRVDGESLCDDCKGKKHLEIHTATHSQLPQPHRVAGAMGRLCIYTGDLLVQALRRTRAAGWSVAEAVWSVLWLAVSAPGKAASGTFWWLGSGWYQFVTLISWLNVFLLTRCLRNICKVFVLLLPLLLLLGAGVSLWGQGNFFSLLPVLNWTAMQPTQRVDDSKGMHRPGPLPPSPPPKVDHKASQWPQESDMGQKVASLSAQCHNHDERLAELTVLLQKLQIRVDQVDDGREGLSLWVKNVVGQHLQEMGTIEPPDAKTDFMTFHHDHEVRLSNLEDVLRKLTEKSEAIQKELEETKLKAGSRDEEQPLLDRVQHLELELNLLKSQLSDWQHLKTSCEQAGARIQETVQLMFSEDQQGGSLEWLLEKLSSRFVSKDELQVLLHDLELKLLQNITHHITVTGQAPTSEAIVSAVNQAGISGITEAQAHIIVNNALKLYSQDKTGMVDFALESGGGSILSTRCSETYETKTALLSLFGVPLWYFSQSPRVVIQPDIYPGNCWAFKGSQGYLVVRLSMKIYPTTFTMEHIPKTLSPTGNISSAPKDFAVYGLETEYQEEGQPLGRFTYDQEGDSLQMFHTLERPDQAFQIVELRVLSNWGHPEYTCLYRFRVHGEPIQ DIP protein Q9D666 original sequence version: 111 B9872C8F2E044964 dctn1_mouse Dynactin subunit 1 150 kDa dynein-associated polypeptide DAP-150 p150-glued Dctn1 protein protein mouse Mus musculus Mouse MAQSRRHMSSRTPSGSRMSTEASARPLRVGSRVEVIGKGHRGTVAYVGATLFATGKWVGVILDEAKGKNDGTVQGRKYFTCDEGHGIFVRQSQIQVFEDGADTTSPETPDSSASKVLKREGADAAAKTSKLRGLKPKKAPTARKTTTRRPKPTRPASTGVAGPSSSLGPSGSASAGELSSSEPSTPAQTPLAAPIIPTPALTSPGAAPPLPSPSKEEEGLRAQVRDLEEKLETLRLKRSEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQPPPETFDFKIKFAETKAHAKAIEMELRQMEVAQANRHMSLLTAFMPDSFLRPGGDHDCVLVLLLMPRLICKAELIRKQAQEKFDLSENCSERPGLRGAAGEQLSFAAGLVYSLSLLQATLHRYEHALSQCSVDVYKKVGSLYPEMSAHERSLDFLIELLHKDQLDETVNVEPLTKAIKYYQHLYSIHLAEQPEDSTMQLADHIKFTQSALDCMGVEVGRLRAFLQGGQEATDIALLLRDLETSCSDTRQFCKKIRRRMPGTDAPGIPAALAFGSQVSDTLLDCRKHLTWVVAVLQEVAAAAAQLIAPLAENEGLPVAALEELAFKASEQIYGSPSSSPYECLRQSCTILISTMNKLATAMQEGEYDAERPPSKPPPVELRAAALRAEITDAEGLGLKLEDRETVIKELKKSLKIKGEELSEANVRLSLLEKKLDSAAKDADERIEKVQTRLDETQTLLRKKEKDFEETMDALQADIDQLEAEKAELKQRLNSQSKRTIEGLRGPPPSGIATLVSGIAGEEPQRGGAPGQAPGALPGPGLVKDSPLLLQQISAMRLHISQLQHENSILRGAQMKASLAALPPLHVAKLSLPPHEGPGGNLVAGALYRKTSQLLEKLNQLSTHTHVVDITRSSPAAKSPSAQLMEQVAQLKSLSDTIEKLKDEVLKETVTQRPGATVPTDFATFPSSAFLRAKEEQQDDTVYMGKVTFSCAAGLGQRHRLVLTQEQLHQLHSRLIS DIP protein O08788 original sequence version: 106 A6CEDCAAA7022EB5 sun1_human SUN domain-containing protein 1 KIAA0810 UNC84A Protein unc-84 homolog A Sad1/unc-84 protein-like 1 SUN1 protein protein human Homo sapiens Human MDFSRLHMYSPPQCVPENTGYTYALSSSYSSDALDFETEHKLDPVFDSPRMSRRSLRLATTACTLGDGEAVGADSGTSSAVSLKNRAARTTKQRRSTNKSAFSINHVSRQVTSSGVSHGGTVSLQDAVTRRPPVLDESWIREQTTVDHFWGLDDDGDLKGGNKAAIQGNGDVGAAAATAHNGFSCSNCSMLSERKDVLTAHPAAPGPVSRVYSRDRNQKCYFLLQILRRIGAVGQAVSRTAWSALWLAVVAPGKAASGVFWWLGIGWYQFVTLISWLNVFLLTRCLRNICKFLVLLIPLFLLLAGLSLRGQGNFFSFLPVLNWASMHRTQRVDDPQDVFKPTTSRLKQPLQGDSEAFPWHWMSGVEQQVASLSGQCHHHGENLRELTTLLQKLQARVDQMEGGAAGPSASVRDAVGQPPRETDFMAFHQEHEVRMSHLEDILGKLREKSEAIQKELEQTKQKTISAVGEQLLPTVEHLQLELDQLKSELSSWRHVKTGCETVDAVQERVDVQVREMVKLLFSEDQQGGSLEQLLQRFSSQFVSKGDLQTMLRDLQLQILRNVTHHVSVTKQLPTSEAVVSAVSEAGASGITEAQARAIVNSALKLYSQDKTGMVDFALESGGGSILSTRCSETYETKTALMSLFGIPLWYFSQSPRVVIQPDIYPGNCWAFKGSQGYLVVRLSMMIHPAAFTLEHIPKTLSPTGNISSAPKDFAVYGLENEYQEEGQLLGQFTYDQDGESLQMFQALKRPDDTAFQIVELRIFSNWGHPEYTCLYRFRVHGEPVK 1099A50C9540ECF5 sgo2_mouse Shugoshin 2 Sgo2 Sgol2 Sgol2a Shugoshin-2 Shugoshin-like 2 protein protein mouse Mus musculus Mouse MEYPGIKVDTVTSGIQRRVKGRIAKTNLNVSLASKIKAKILNNSSIFKISLKHNNRALARALSKEKENSRRITTEKMQLQKEVEKLNFENTFLRLKLNTLNKKLVEIESHVSNDLLTAIEISSLSEFHQGSFLLSATKKQRNSKQCKPAHLPYARVLLTSENDDDDGADDKWQTKCNNRTISKTSPDSTSSVSRQPSSLHQCNLKAFPPKEDNQKTCGSGHLEHTSSVDILPNESHSDQSPKSSLSEMKTAPSPSLRREKLSHGNVTMRKKCVSSTPDILYVTDLDHQPTSSPGSNWNNEIHGHTNETSNNTQRNAECFLDLPSESSSEPDAKRMELVQKNTDSFHFQKTVYDAADMELTATDIGKIVAVSKSKKNQNKKKADCRKETFRKVKGASSDKKRESSKRECKDGSEVGAEEEADAARAERGAGVLDGRGDSEEPNCISSTEQPSQVNTQKKRTLQNSSDQENIQNTKRRQTYTTDEQEETNPFSRHSVKFLQDGKFDLCQKTLHHNLSKPSRQTFVIRKSEKDNLFPNQEDKDTISENLEVTNEFHIDDLSIEANENVCDHETQTMLDLKKSVSAQQNQTKINKTKQKINRRTKIISVMSQVYEDNDKDIHVLEKDNFPFHTQANKETTSGNLESSKEFESPLLFTRDNGSLRDCKTQNVLDLHKQIPDLYPDRNESQISKIPRQKVNRKTEVISGVKCFSNDQGVHCSEKDKSLLLQKDKDFPGTLKDLSEFDTPAFCNKDSAKSCDYKSEMLLGLKKHDPNMQPACQDDSKAGKKLRQKVNRKTEIISKITQIHENDRGSTHDSLNKKLCQKVNISKIISQMNQIYETINEDGNGFKSSIKDCEDIKSCDFGEINSNKKENYDPIQDPCTLVKKTKRKGSCKAGSSLAGAKNRCGLQLTDSSQVQSVPLDSGLRHHPNEADSGPGEQTNLPKMQKQSAGRSLGDAFSVSLGKEGSRPAKAVSKMTPKSKKRKLPLGCSPETHGTVEITPNTDLAKAVDSQQTEKENYLEKEKIAKRKPDFCTKVLKPLSETCSSNIKNSSLDSMCKSSLPLSISSRKTLMLEESSSLESTCIFQVGDAAHEKITTGTRNPHHRTQKSTPGSRTSLVLVDTSSVSDTNPANPENESEGQSSHPMRRKRQCVPLNLTEPSLRSKMRR 64BCDCE5738576D6 kash5_mouse Protein KASH5 Kash5 Ccdc155 Coiled-coil domain-containing protein 155 KASH domain-containing protein 5 protein protein mouse Mus musculus Mouse MHSILRSSLSSREALRMRQLKGLRKERPGRHPLGVRLRAIWTSFLFPNPPHSGGKLRASTAAVEEHQEWSMDLPEGQAGGPTAQMYLWEQPEEASSRPLLSLEEQILNSTFEACDPHKTGTVTVAHLLAYLEAVTGQGPQDVRLQTLARSLDPYGEGAGATVELDTFLVVMRDWIAACQLQGGLERAEETAYEGALASPHLPSVCPEAEESANLESFGGEDPRPEGPATAELLSNLEDLELSNRRLAGENAKLQRSVETAEEGSARLGEEITALRKQLRSTQQALQVAKALDEELEDLKTLAKSLEEQNRSLMAQARHTEKEQQHLAAEVETLQEENEKLLAERDGVKRRSEELATEKDALKRQLCECERLICQREAVLSERTRHAESLARTLEEYRTTTQELRQEISNLEEQLSQSQEGPEELLEGAEAGRVGWIMALPPSLDLEIQAIRQEQDVASAGLSSPLYGVWQWEEVEPEPEPEPEPEPEPEPQEVEFPSEDPARQQTDLQREPVRALEGSRAPCLRLSRSQEEEEEEEESWVLADPSSPLGTYHHKLAPGSSRESCHIVPEMHQALMPVVRDLVPVERSRTQHCLHPQHSPGIRISQHPLVPTPVLGLLLLLLLSILLFSQSPPPTWPHLQLYYLQPPPV DIP protein Q80VJ8 original sequence version: 77 65CC342EB6F9DA51 sun1-ccdc155-1 2 7 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 3A ccdc155-sun1-1 1 11 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q80VJ8:p.Pro645_Pro647delinsAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain PPP AAA Fri Jul 22 11:08:13 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'pro645ala,pr0646ala,pro647ala' Wed May 02 19:53:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_pro_pro645-647ala_ala_ala' Tue Jun 30 18:58:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro645_Pro647delinsAlaAlaAla' 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 17 20 physical association physical association 3B ccdc155-sun2-1 1 11 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q80VJ8:p.Pro645_Pro647delinsAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain PPP AAA Fri Jul 22 11:00:36 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'pro645ala,pr0646ala,pro647ala' Wed May 02 16:50:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_pro_pro645-647ala_ala_ala' Tue Jun 30 19:32:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro645_Pro647delinsAlaAlaAla' 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 23 26 physical association physical association 3B sun1-ccdc155-2 2 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey association association 5A sun1-ccdc155-3 5 7 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization 2C sgo2-ccdc155-1 3 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association no figure ccdc155-sun1-2 4 9 unspecified role unspecified role neutral component neutral component 11 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q80VJ8:p.Pro645_Pro647delinsAlaAlaAla mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain PPP AAA Wed May 02 15:10:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_pro_pro645-647ala_ala_ala' Tue Jun 30 20:10:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro645_Pro647delinsAlaAlaAla' Q80VJ8:p.Pro645_Pro647del mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain PPP ... Wed May 02 16:28:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_pro_pro645-647del_del_del' Tue Jun 30 15:43:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro645_Pro647del' colocalization colocalization supplemental S3B and S3A