IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. The Journal of experimental medicine 2012 imex curation Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu Accepted 2016-MAY-16 AT 12:35 BST AT 12:35 BST by ORCHARD The Journal of experimental medicine 2012 Only protein-protein interactions imex curation imex curation Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. The Journal of experimental medicine 2012 imex curation Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-m059k Human glioblastoma M059JK cells m059k Human glioblastoma M059K cells These cells express DNA-dependent protein kinase (DNA-PK) http://www.lgcstandards-atcc.org/products/all/CRL-2365.aspx?geo_country=gb#generalinformation anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu Accepted 2016-MAY-16 AT 12:35 BST AT 12:35 BST by ORCHARD The Journal of experimental medicine 2012 Only protein-protein interactions imex curation imex curation Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. The Journal of experimental medicine 2012 imex curation Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps tag fluorescence tag visualisation by fluorescence tag fluorescence Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu Accepted 2016-MAY-16 AT 12:35 BST AT 12:35 BST by ORCHARD The Journal of experimental medicine 2012 Only protein-protein interactions imex curation imex curation Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. The Journal of experimental medicine 2012 imex curation Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-n114p2 human N114P2- Ligase IV–deficient pre-B cell line Human n114p2 N114P2- Ligase IV–deficient pre-B cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu Accepted 2016-MAY-16 AT 12:35 BST AT 12:35 BST by ORCHARD The Journal of experimental medicine 2012 Only protein-protein interactions imex curation imex curation Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. The Journal of experimental medicine 2012 imex curation Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-nalm_6 Homo sapiens NALM-6 B cell precursor leukemia cells nalm_6 Human B cell precursor leukemia NALM-6 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu Accepted 2016-MAY-16 AT 12:35 BST AT 12:35 BST by ORCHARD The Journal of experimental medicine 2012 Only protein-protein interactions imex curation imex curation Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. The Journal of experimental medicine 2012 imex curation Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-m059j M059J-Human glioblastoma cell line m059j M059J-Human glioblastoma cell line http://www.lgcstandards-atcc.org/products/all/CRL-2366.aspx lacks DNA-dependent protein kinase activity. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu Accepted 2016-MAY-16 AT 12:35 BST AT 12:35 BST by ORCHARD The Journal of experimental medicine 2012 Only protein-protein interactions imex curation imex curation Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. Artemis C-terminal region facilitates V(D)J recombination through its interactions with DNA Ligase IV and DNA-PKcs. The Journal of experimental medicine 2012 imex curation Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. Dark Space = no 23? patricia.cortes@mssm.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Malu S., De Ioannes P., Kozlov M., Greene M., Francis D., Hanna M., Pena J., Escalante CR., Kurosawa A., Erdjument-Bromage H., Tempst P., Adachi N., Vezzoni P., Villa A., Aggarwal AK., Cortes P. I might be "seeing things" but Fig 2A (2B) has Artemis with a C term Flag tag, but Fig 3A (3B) shows an N- term Flag tag? Dark Space = no 23? patricia.cortes@mssm.edu Accepted 2016-MAY-16 AT 12:35 BST AT 12:35 BST by ORCHARD The Journal of experimental medicine 2012 Only protein-protein interactions imex curation imex curation prkdc_human DNA-dependent protein kinase catalytic subunit PRKDC HYRC HYRC1 DNPK1 p460 protein protein human Homo sapiens Human MAGSGAGVRCSLLRLQETLSAADRCGAALAGHQLIRGLGQECVLSSSPAVLALQTSLVFSRDFGLLVFVRKSLNSIEFRECREEILKFLCIFLEKMGQKIAPYSVEIKNTCTSVYTKDRAAKCKIPALDLLIKLLQTFRSSRLMDEFKIGELFSKFYGELALKKKIPDTVLEKVYELLGLLGEVHPSEMINNAENLFRAFLGELKTQMTSAVREPKLPVLAGCLKGLSSLLCNFTKSMEEDPQTSREIFNFVLKAIRPQIDLKRYAVPSAGLRLFALHASQFSTCLLDNYVSLFEVLLKWCAHTNVELKKAALSALESFLKQVSNMVAKNAEMHKNKLQYFMEQFYGIIRNVDSNNKELSIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTQTDTGDDRVYQMPSFLQSVASVLLYLDTVPEVYTPVLEHLVVMQIDSFPQYSPKMQLVCCRAIVKVFLALAAKGPVLRNCISTVVHQGLIRICSKPVVLPKGPESESEDHRASGEVRTGKWKVPTYKDYVDLFRHLLSSDQMMDSILADEAFFSVNSSSESLNHLLYDEFVKSVLKIVEKLDLTLEIQTVGEQENGDEAPGVWMIPTSDPAANLHPAKPKDFSAFINLVEFCREILPEKQAEFFEPWVYSFSYELILQSTRLPLISGFYKLLSITVRNAKKIKYFEGVSPKSLKHSPEDPEKYSCFALFVKFGKEVAVKMKQYKDELLASCLTFLLSLPHNIIELDVRAYVPALQMAFKLGLSYTPLAEVGLNALEEWSIYIDRHVMQPYYKDILPCLDGYLKTSALSDETKNNWEVSALSRAAQKGFNKVVLKHLKKTKNLSSNEAISLEEIRIRVVQMLGSLGGQINKNLLTVTSSDEMMKSYVAWDREKRLSFAVPFREMKPVIFLDVFLPRVTELALTASDRQTKVAACELLHSMVMFMLGKATQMPEGGQGAPPMYQLYKRTFPVLLRLACDVDQVTRQLYEPLVMQLIHWFTNNKKFESQDTVALLEAILDGIVDPVDSTLRDFCGRCIREFLKWSIKQITPQQQEKSPVNTKSLFKRLYSLALHPNAFKRLGASLAFNNIYREFREEESLVEQFVFEALVIYMESLALAHADEKSLGTIQQCCDAIDHLCRIIEKKHVSLNKAKKRRLPRGFPPSASLCLLDLVKWLLAHCGRPQTECRHKSIELFYKFVPLLPGNRSPNLWLKDVLKEEGVSFLINTFEGGGCGQPSGILAQPTLLYLRGPFSLQATLCWLDLLLAALECYNTFIGERTVGALQVLGTEAQSSLLKAVAFFLESIAMHDIIAAEKCFGTGAAGNRTSPQEGERYNYSKCTVVVRIMEFTTTLLNTSPEGWKLLKKDLCNTHLMRVLVQTLCEPASIGFNIGDVQVMAHLPDVCVNLMKALKMSPYKDILETHLREKITAQSIEELCAVNLYGPDAQVDRSRLAAVVSACKQLHRAGLLHNILPSQSTDLHHSVGTELLSLVYKGIAPGDERQCLPSLDLSCKQLASGLLELAFAFGGLCERLVSLLLNPAVLSTASLGSSQGSVIHFSHGEYFYSLFSETINTELLKNLDLAVLELMQSSVDNTKMVSAVLNGMLDQSFRERANQKHQGLKLATTILQHWKKCDSWWAKDSPLETKMAVLALLAKILQIDSSVSFNTSHGSFPEVFTTYISLLADTKLDLHLKGQAVTLLPFFTSLTGGSLEELRRVLEQLIVAHFPMQSREFPPGTPRFNNYVDCMKKFLDALELSQSPMLLELMTEVLCREQQHVMEELFQSSFRRIARRGSCVTQVGLLESVYEMFRKDDPRLSFTRQSFVDRSLLTLLWHCSLDALREFFSTIVVDAIDVLKSRFTKLNESTFDTQITKKMGYYKILDVMYSRLPKDDVHAKESKINQVFHGSCITEGNELTKTLIKLCYDAFTENMAGENQLLERRRLYHCAAYNCAISVICCVFNELKFYQGFLFSEKPEKNLLIFENLIDLKRRYNFPVEVEVPMERKKKYIEIRKEAREAANGDSDGPSYMSSLSYLADSTLSEEMSQFDFSTGVQSYSYSSQDPRPATGRFRRREQRDPTVHDDVLELEMDELNRHECMAPLTALVKHMHRSLGPPQGEEDSVPRDLPSWMKFLHGKLGNPIVPLNIRLFLAKLVINTEEVFRPYAKHWLSPLLQLAASENNGGEGIHYMVVEIVATILSWTGLATPTGVPKDEVLANRLLNFLMKHVFHPKRAVFRHNLEIIKTLVECWKDCLSIPYRLIFEKFSGKDPNSKDNSVGIQLLGIVMANDLPPYDPQCGIQSSEYFQALVNNMSFVRYKEVYAAAAEVLGLILRYVMERKNILEESLCELVAKQLKQHQNTMEDKFIVCLNKVTKSFPPLADRFMNAVFFLLPKFHGVLKTLCLEVVLCRVEGMTELYFQLKSKDFVQVMRHRDDERQKVCLDIIYKMMPKLKPVELRELLNPVVEFVSHPSTTCREQMYNILMWIHDNYRDPESETDNDSQEIFKLAKDVLIQGLIDENPGLQLIIRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTSMSPDYPNPMFEHPLSECEFQEYTIDSDWRFRSTVLTPMFVETQASQGTLQTRTQEGSLSARWPVAGQIRATQQQHDFTLTQTADGRSSFDWLTGSSTDPLVDHTSPSSDSLLFAHKRSERLQRAPLKSVGPDFGKKRLGLPGDEVDNKVKGAAGRTDLLRLRRRFMRDQEKLSLMYARKGVAEQKREKEIKSELKMKQDAQVVLYRSYRHGDLPDIQIKHSSLITPLQAVAQRDPIIAKQLFSSLFSGILKEMDKFKTLSEKNNITQKLLQDFNRFLNTTFSFFPPFVSCIQDISCQHAALLSLDPAAVSAGCLASLQQPVGIRLLEEALLRLLPAELPAKRVRGKARLPPDVLRWVELAKLYRSIGEYDVLRGIFTSEIGTKQITQSALLAEARSDYSEAAKQYDEALNKQDWVDGEPTEAEKDFWELASLDCYNHLAEWKSLEYCSTASIDSENPPDLNKIWSEPFYQETYLPYMIRSKLKLLLQGEADQSLLTFIDKAMHGELQKAILELHYSQELSLLYLLQDDVDRAKYYIQNGIQSFMQNYSSIDVLLHQSRLTKLQSVQALTEIQEFISFISKQGNLSSQVPLKRLLNTWTNRYPDAKMDPMNIWDDIITNRCFFLSKIEEKLTPLPEDNSMNVDQDGDPSDRMEVQEQEEDISSLIRSCKFSMKMKMIDSARKQNNFSLAMKLLKELHKESKTRDDWLVSWVQSYCRLSHCRSRSQGCSEQVLTVLKTVSLLDENNVSSYLSKNILAFRDQNILLGTTYRIIANALSSEPACLAEIEEDKARRILELSGSSSEDSEKVIAGLYQRAFQHLSEAVQAAEEEAQPPSWSCGPAAGVIDAYMTLADFCDQQLRKEEENASVIDSAELQAYPALVVEKMLKALKLNSNEARLKFPRLLQIIERYPEETLSLMTKEISSVPCWQFISWISHMVALLDKDQAVAVQHSVEEITDNYPQAIVYPFIISSESYSFKDTSTGHKNKEFVARIKSKLDQGGVIQDFINALDQLSNPELLFKDWSNDVRAELAKTPVNKKNIEKMYERMYAALGDPKAPGLGAFRRKFIQTFGKEFDKHFGKGGSKLLRMKLSDFNDITNMLLLKMNKDSKPPGNLKECSPWMSDFKVEFLRNELEIPGQYDGRGKPLPEYHVRIAGFDERVTVMASLRRPKRIIIRGHDEREHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSACSQRALQLRTYSVVPMTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTKMSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPEAFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHAFGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDPGLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKICYAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPESGLSEETQVKCLMDQATDPNILGRTWEGWEPWM DIP protein P78527 original sequence version: 118 DIP protein P78527-1 original sequence version: 117 DIP protein P78527 original sequence version: 170 DIP protein P78527 original sequence version: 179 DIP protein P78527 original sequence version: 114 DIP protein P78527 original sequence version: 125 AC6E747FEB09F3E5 dnli4_human DNA ligase 4 DNA ligase IV Polydeoxyribonucleotide synthase [ATP] 4 LIG4 protein protein human Homo sapiens Human MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAIADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNEAIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI DIP protein P49917 original sequence version: 127 DIP protein P49917 original sequence version: 151 DIP protein P49917 original sequence version: 144 2122813E1EFA63B9 xrcc4_human DNA repair protein XRCC4 X-ray repair cross-complementing protein 4 XRCC4 protein protein human Homo sapiens Human MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI DIP protein Q13426 original sequence version: 140 DIP protein Q13426-1 original sequence version: 116 BE5FB99153479A4E dcr1c_human Protein artemis DNA cross-link repair 1C protein Protein A-SCID SNM1 homolog C SNM1-like protein DCLRE1C ARTEMIS ASCID SCIDA SNM1C protein protein human Homo sapiens Human MSSFEGQMAEYPTISIDRFDRENLRARAYFLSHCHKDHMKGLRAPTLKRRLECSLKVYLYCSPVTKELLLTSPKYRFWKKRIISIEIETPTQISLVDEASGEKEEIVVTLLPAGHCPGSVMFLFQGNNGTVLYTGDFRLAQGEAARMELLHSGGRVKDIQSVYLDTTFCDPRFYQIPSREECLSGVLELVRSWITRSPYHVVWLNCKAAYGYEYLFTNLSEELGVQVHVNKLDMFRNMPEILHHLTTDRNTQIHACRHPKAEEYFQWSKLPCGITSRNRIPLHIISIKPSTMWFGERSRKTNVIVRTGESSYRACFSFHSSYSEIKDFLSYLCPVNAYPNVIPVGTTMDKVVEILKPLCRSSQSTEPKYKPLGKLKRARTVHRDSEEEDDYLFDDPLPIPLRHKVPYPETFHPEVFSMTAVSEKQPEKLRQTPGCCRAECMQSSRFTNFVDCEESNSESEEEVGIPASLQGDLGSVLHLQKADGDVPQWEVFFKRNDEITDESLENFPSSTVAGGSQSPKLFSDSDGESTHISSQNSSQSTHITEQGSQGWDSQSDTVLLSSQERNSGDITSLDKADYRPTIKENIPASLMEQNVICPKDTYSDLKSRDKDVTIVPSTGEPTTLSSETHIPEEKSLLNLSTNADSQSSSDFEVPSTPEAELPKREHLQYLYEKLATGESIAVKKRKCSLLDT 24B857F5B473637B 678s475e6ye-1 5 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association 1A dclre1c-lig4-3 4 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association 1A dclre1c-lig4-5 6 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 1B dclre1c-lig4-6 2 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey association association 1B dclre1c-lig4-7 7 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2B, dclre1c-lig4-8 7 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait n_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2 D/F lig4-dclre1c-3 7 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait c_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 2Di dclre1c-lig4-9 1 9 unspecified role unspecified role prey prey dbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 44 48 direct interaction direct interaction 2E dclre1c-lig4-10 7 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 3B, 3D dclre1c-lig4-11 7 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3D dclre1c-lig4-12 7 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q96SD1:p.[Leu401_Arg402delinsGlyAsn;Trp489Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain LR GN certain certain sequence position certain certain certain sequence position certain W A Fri Jul 22 10:51:14 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu401gly,arg402asn,trp489ala' Wed May 02 16:18:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_arg401-402gly_asn,trp489ala' Tue Jun 30 18:21:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu401_Arg402delinsGlyAsn;Trp489Ala]' association association 3F, 4A dclre1c-lig4-13 7 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q96SD1:p.Phe492Ala mutation mutation certain certain sequence position certain certain certain sequence position certain F A Wed May 02 16:06:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe492ala' Tue Jun 30 19:47:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe492Ala' Q96SD1:p.Trp489Ala mutation mutation certain certain sequence position certain certain certain sequence position certain W A Wed May 02 18:26:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp489ala' Tue Jun 30 18:05:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp489Ala' Q96SD1:p.Phe493Ala mutation mutation certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:03:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe493ala' Tue Jun 30 17:44:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe493Ala' physical association physical association 3Fi, 4A dclre1c-1 3 9 unspecified role unspecified role neutral component neutral component dbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role neutral component neutral component Q96SD1:p.Trp489Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain W A Wed May 02 20:13:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp489ala' Tue Jun 30 19:41:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp489Ala' region fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q96SD1:p.Phe492Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 16:55:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe492ala' Tue Jun 30 16:09:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe492Ala' Q96SD1:p.Phe493Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain F A Wed May 02 18:59:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe493ala' Tue Jun 30 18:05:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe493Ala' 80 76 direct interaction direct interaction 3H dclre1c-lig4-14 7 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q96SD1:p.Leu401_Arg402delinsGlyAsn mutation mutation certain certain sequence position certain certain certain sequence position certain LR GN Fri Jul 22 10:58:41 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu401gly,arg402asn' Wed May 02 20:12:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_arg401-402gly_asn' Tue Jun 30 16:05:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu401_Arg402delinsGlyAsn' association association 4A