UniProt UniProt http://www.uniprot.org Samp1 is a component of TAN lines and is required for nuclear movement. Samp1 is a component of TAN lines and is required for nuclear movement. Journal of cell science 2012 imex curation Borrego-Pinto J., Jegou T., Osorio DS., Auradé F., Gorjánácz M., Koch B., Mattaj IW., Gomes ER. edgar.gomes@upmc.fr 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Borrego-Pinto J., Jegou T., Osorio DS., Auradé F., Gorjánácz M., Koch B., Mattaj IW., Gomes ER. GFP-SUN2 was provided by Howard Worman (according materials and methods section) who described the plasmid in PMID 19843581. GFP-mini-nesprin-2G was provieded by Gregg Gundersen who described the contruct in PMID 20724637. edgar.gomes@upmc.fr Accepted 2016-DEC-12 AT 13:36 GMT AT 13:36 GMT by MGT Journal of cell science 2012 imex curation Samp1 is a component of TAN lines and is required for nuclear movement. Samp1 is a component of TAN lines and is required for nuclear movement. Journal of cell science 2012 imex curation Borrego-Pinto J., Jegou T., Osorio DS., Auradé F., Gorjánácz M., Koch B., Mattaj IW., Gomes ER. edgar.gomes@upmc.fr 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Borrego-Pinto J., Jegou T., Osorio DS., Auradé F., Gorjánácz M., Koch B., Mattaj IW., Gomes ER. Fusion construct GFP-mini-nesprin2G provided by Gregg Gundersen who described the construct in PMID 20724637 edgar.gomes@upmc.fr Accepted 2016-DEC-12 AT 13:36 GMT AT 13:36 GMT by MGT Journal of cell science 2012 imex curation Samp1 is a component of TAN lines and is required for nuclear movement. Samp1 is a component of TAN lines and is required for nuclear movement. Journal of cell science 2012 imex curation Borrego-Pinto J., Jegou T., Osorio DS., Auradé F., Gorjánácz M., Koch B., Mattaj IW., Gomes ER. edgar.gomes@upmc.fr 21-Mar-2017: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Borrego-Pinto J., Jegou T., Osorio DS., Auradé F., Gorjánácz M., Koch B., Mattaj IW., Gomes ER. Fusion construct GFP-mini-nesprin2G provided by Gregg Gundersen who described the construct in PMID 20724637 edgar.gomes@upmc.fr Accepted 2016-DEC-12 AT 13:36 GMT AT 13:36 GMT by MGT Journal of cell science 2012 imex curation syne2_mouse Nesprin-2 Nuclear envelope spectrin repeat protein 2 Nucleus and actin connecting element protein Synaptic nuclear envelope protein 2 KASH domain-containing protein 2 Syne2 protein protein mouse Mus musculus Mouse MAASPVLPTEDGEGFLGIDDLHFSLQAEQEDTQKKTFTCWINSQLAKHTPPSVVSDLFADIKKGHVLLDLLEVLSGQQLPRDKGSNTFQCRINIEHALTFLKNRSIKLINIHVADIVEGNPSIILGLIWTIILHFHIEKLAQTLSCDYNQPSPEVVSVAASSPTSSPPTKKCSKAQAQARWQWSAKKALLQWAQEQCARSESVNVTDFKSSWRNGMAFLAVIHALRPDLIDMDSMRHRSNKDNLKEAFRIAEHELKIPKLLEPEDVDVVNPDEKSIMTYVAQFLKYSKDAPGPGDSTQAKVRDALVWLTLQEKRFQKMLKDSASETYCNKYHSLLSFMESLNEEKESFIDVLSLKGRMGELNEDESRLRQGWTSLMHQVAAWRAQLDDALPSPLKETEAWLKDIEGVVQEGVPTSQSYSEARTLIQGKLSSFKSLMGSFDYHSDVLMAFQSNAEKSLPAVPPVKLEEMTRRINNVLGKNFIPLLEFHDSKCSVLALLDEAKAKLDVWNGTYESKESVEVLLEDWHKFTGEKKFLIQLDASFQKCEEMYKNSARECESIREEYMMLEKNVHSCRQYIHNTKATLQRALMSWATFEEDLALLKASFDLTKKEQIKEVPVETLLQWNTKHTSLNEVGSFLIGVSSREVAASISKELRRLNKRWRKFITKTPLLKLPLVKIQDQPPGNSSGTSLSKESAMAAEPGGSRGEDVKAAEKQEVEDEESAGQLKVNEEVEGLIKQVTIWESQTKSILDLLQHGDHADGSSADTLQHLIAKGSVYEELLARTEDTLQMDVQSPSNLEPFQNVLRAGLQAKIQEAKQGVQITMVELSAVLKNLSDEPLELDLGLKVEEAQKELEVSILRAEQLLGQRERPGGFLLKYKEALEILNTNSLAKYLRAVEELKRTVPGGAKLQLEEQSRVASAKWEPLRHEISLYLQQLKIAIEEEKLRDNIARLEKQINKEKKLIRRGRTRGLRKEHEACLSPESIKCQLEHHVGVLRVLCEELTSPEDQQELKRALRDYEQKIARLLKCASEIHTTLQSSQGGALEERSALITTENGRRDADGEVPLEIPDNQLSTEKAMEPIKNFSQTSELKPQQEESIMEKEGKDCSASLSDLQERYDTQRGLLEQHLQDSKSRVTSDFASEQERSSACLQSKLAELQVLLADTDAHWEKFEITSLNLRRLMSDAEKPVLNQERDLLKGNEQVLHGLLNTRMESLEMALQIVLPLEKECSLLCASDLPLCTVAVQDLHPVEIDGVYQNLRDIRDSIAKQIRVCTSLEEPSNSVPRELHTLDQCAIQDIVLKCRLQLETMNQKVEMREDALDALEGFLASLRAAKLSAELPADRPAPKAPEVLSEDILLMKEKAGPLDERLRTLGINIKDAEGGENTTCERLVGALSVNLVAMDGQSKEEGPPEDKKLLEACSSKNLELFKNIQDLQNQISKIGLKDPTAPAVKHRKKSLLRLDKDLDGLEEEKVRIQKIAGSLPRFKDGSEKNVIQQCEDTAALWESTKASVTESLEQCGSALELLRQYQNIKNNLTALIQKEEGIISQQASYMGKDNLKKKIAEIETVKEEFSDHLEVVDKINQICKNLQYHLNKMKTFEDPPFEKEANAIVDRWLDINEKTEEYGENLGRALALWDKLFIIKNNIDEWTEQILGKAESHELTEEDRGRLKEELKVLEEQSAEFSRRVADIQSLLQSNEKPLELQVMESSVLSKMKDVKTHVAGGSNSYAPSGSTAELREDLDQAKTQMGMTESLLNALSPSDSLEIFTKLEEIQQQIFQQKHSMTVLENQIGCLTPELSELKRQYASVSNLFNTKKNALQDHFATFLNEQCKNFNDWFSNVKTNLQECFEPPETKLSLEQRLQKLSDFLTLGGGNSKIQQVETVLQHVKMLLPKAHVKELDSWLRSQELELENMESICQARAGELNNSFQQLLRLEDDCRSLSKWLTNQEENWGKMEVSGERMDLFSQALTRKREQFETVAQLSDSLKEHGLTEGEETIKESTHLIDRYQALWRQLHEIEEEDKLPAAEDQSFNDLADDVIHWIKEIKESLMALNSSEGKMPLEERIQKIKEIIALKPEGDAKIQMVMRQAEHCEAPLAQETFTDLSNQWDSTLHLANTYLSHQEKLVLEGEKYLQSKEDLRLMLTELKKQQEAGFALQPGLPEKQAQLKIYKKFLQKAQDLTSLLEELKSQGNYLLECTKNPSFSEEPWLEVKHLHESLLQQLQDSVQKLEGHVQEHSSYQVCLTDLSSTLDDISKEYFSLCDGSKDQIMAKERMQKLQELESRLRFQGGALKKASALAKSIKQNTSSVGQKIIKDDIRSLKYKQKDLENRIESAKQETENGLNSILKSKSSTEKHVKFSLPVEEMPATSEVPKPTRESAAVGESGGARETNTNSAVEMILSKQLSLNVQESMQNAQDEREVNELQNQPLELDIMLRNEQLKGMEELSTHLEARRAAIELLEQSQHLNQTEEQALVLPAARPSVCHLGSLQQELHTLKKTKERQYGLLSGFQDQLVMAEASLNTSLAEVESLKIGSLDSATYLGKIKKFLGSVENQQGSLSKLRTEWAHLSSLLAAADQKLVESQMKHLEHGWELVEQLAHRKCFQQATEHSELTCLLEKLQDLKVSLHQQQQRLTLSLNSPGQQAAIVDMVTPAAELQAIKCEFSGLKWQAELHMKRLWGEKDKKTLEDAINNLNKQMEALEPLNREVENRIKKCELQNRIKETLSWVKNTMAELVVPIALLPDNILSQIRKCKLIHDGILGNQQAVELLVEEVRGITPSLAPCEGDGLNALLEDLQSQHQALLLKSTERSQQLELKLEEKSKLFAIIGKVQLTLEESETLMSPTGDRASTEAELERRLAILKASQQQLQDTESALSAHLQELTNAYKDANVFERLFLDDQLKNLKARTNRTQRFLQNNGSELKQKMESYREFHDKAAVLQKEAECILHGGLLPLRQELEQDAKEQLGNLRDKLAAIRGSLSQVLTSEEVFDTIGLSWDGSLLARLQTQVLEREREVEGKIKQLDTFLIARDRHQASISKIRAVDLQIKKGAESLLKVPSMSPESTLLNAQTLIQKIEKSKRLRDEIIRKLSKNEAFDDSFKESEMQRLKLCAEENSRLQEALQNMLLELQPREMGEKEFREKLENALHVLKQIQSRLQQPLCVNLGVQHIQHEKETWEAFGEQVEAEMCGLRAVRITEEQREENDSGTGGMEAKLRDIEGLHMELSKSISLRADVLNDAYDSANRYDELVAGALRIITSLEATLLSYRVDLHNPQKTLELAHLKQEELQSSVADLRSLTETLGAISSPEAKEQLRCTLEVLAAKNSALKAGLEAQEAEEERCLENYKCFRKMKEEICSRLRKMEMDLGQSIFPLPRSYKEALARLEQSKALTSNLLSTKEDLVKLRQLLRHLRCRSTENDATCALGVASALWEKWLSLLEAAREWQQWGGELKREWKFISEEIEREAIILETLQEDLPEISKTNAAPTEELWQLLDSLCQHQESVEKQQLLLALLLQRVRSIQNIPEGTETGETIPALQEIGSMQERCDRLLHTTRKNKDLVQAEIQAQQSFLKEIKDVKRVFEQISTSFPNLAPEGHPERAEQFEELRSILQKGKLSFENIMEKLRIKYSEMYSIVPAEIGSQVEECRSALEDAEEKMSSEVSKSSPSSIMRRKIERINNGLHCVEKMLQQKSRNIEEAHEIQKKIWDELDLWHSKLNELDSEVQDFVEQDPGQAQEWMDNLMAPFQQHQQVSQRAESRTSQLNKATIKMEEYNDLLKSTEVWIEKTSCLLANPACYDSSRTLSHRASTLQMALEDSEQKHSLLHSIFTDLEDLSIIFETDDLIQTIHELSDQVAALQQQIMEALPHVQQVADDVVAIESEVKAMEKKVAKIKAILLSKEIFDFPPEEHLKHGEVILENIHPMKKTIAEIMTYQVELRLPQTGTKPLPVFQRTSQLLQDVKLLENVTQEQNELLKVVIKQTAECDEEIDSLKQMLTNYSAEISPEHVSQNQVADLPSLQGEMERLEKQILNLNQKKEDLLVDLKTAVLNLHEHLKQEQQEVGDKPSAGASECTVAERDASERKLSRTNSMSFLPVVKEEAEESSVKSEDGRRRTEPPSASWSFLGKHSKDLEGDGASSSSSATIVQDADGRISTCDSSMVHIIAPDSGSTEEGPAPSPRLSQTDEGATPPIEAALLDFPREQGAFESTVERSRPRPADILRVCKTQVAKLELWLQQANVAFEPETVDADMQQVVEEELAGCQAMLTEIEYKVASLLETCKDQGLGDCGTTQQEAEALSWKLKTVKCNLEKVQMVLQEKFSEDQHPSTLKKPSEPHDVDQPAGLSELDSVLTERPQFSRQKDAPQPQILELKPSEQKDLIKFTELNAKKTWLQGHQENEDANRQSASSSKVPSPGNAASDSTLPLQAQSGDKWQYLHHELTSRPNPSVPQLVEPQVALTTSTLPSVSVYNFRCPTADELQAYTTQLEELRQEANTIQTQGSMTEETYISLDKRLFELFLSLSRCLGSVEGLLQRPGLLREDACAQQVFFQKLALELKKLYLALGDKKDDFLKAVTWPGKEATLLPECIDALTVSLESVQSRAAWRDASLKAGLEHSRSYQNEVKRLYSQLIKKKTALQQSLNEISGQSISKQLQKADVHTAELQNSEKQVAKLRDEGERLRFPHGLLQDVYKLEDVLDSMWGILRARYLELSSPFLSKSLQTLLQGMAELVSIGKGKLAADPLQHAKSKAALQAQLQDHKAFFQKLVADMLLIQTYSATMFPPSLQKGEGFGAEQVAEVRALEEEACLRGAQLQSMLQKWEEFDDNYASLEKDLEALISSLPSVSLVEETEERLLERISFYQQIKRNIDGKHARLCQTLNEGRQLAASVSCPEPEGQIARLEEQWLSLNKRIDQELHRLQTLLKHLLSYSRDSDELTRWLETSQQTLNYWKEQSLNVSQDLNTIRSNIDRFFKFSKEVDERSSLKSAVMSTGNQLLHLKEADTATLRASLAQFEQKWTVLITQLPDIQEKLHQLQMEKLPSREAISEMISWMNAVEPQAAGKDTELSKSSASQVKHLLQKLKEFRMEMDYKQWVVDFVNQSLLQLSTCDVESKRYERTEFAEHLGEMNRQWQRVHGTLNRKIQHLEQLLESITENENKVQNLNSWLEAQEERLKMLQKPESAVSMEKLLLDCQDIENQLALKSKALDELRQSSLTMDGGDVPLLEDMASGIVELFQKKNNVTSQVHQLRASVQSVLQEWKACDKLYDEATMRTTQLTYSMEHSKPAVLSLQALACQVQNLEALQDEAENGERSWEKLQEVIGRLKASCPSMAGIIEEKCQDAHSRWTQVNQDLADQLQEARGQLQLWKAPHNAHAEAAAWLQQQEAKFQQLANTNLSGDNLADILPRALKDIKGLQSDLQKTKEAFLENSTLSDQLPQPEERSTPGLHSGQRHSLQTAAYLEKMLLAKSNEFEIVLAQFKDFTDRLAYSKDLIVHKEENLNKLYHEEKEEVPDLFLNHVLALTAQSPDIERLNEESLRLPLSDVTIKTLQSLNRQWIRATATALDHYSELQGNGLNEKFLHYCERWIQVLEKIQESLSVEVAHSLPALLEQQKTYEILEAEVSTNQAVADAYVTQSLQLLDTAEIEKRPEFVSEFSKLSDQWQRAARGVRQRKCDISRLVTQWRFFTTSVEDLLRFLADTSQLLSAVKEQDCYSLCQTRRLVHELKSKEIHLQRWRTTYALALEAGEKLRNTPSPETREFVDGQISRLQESWKDTELSLGEVISRLQSTAETWDQCKKKIKKLKKRLQALKAQSEDPLPELHEALHEEKELIKEVEKSLANWTHSLKELQTMKADLSQHILAEDVTVLKEQIQLLHRQWEDLCLRVAIRKQEIEDRLNSWIVFNEKNKELCAWLVQMENKVLQTADVSIEEMIEKLQKDCMEEISLFTENKLQLKQMGDQLIKASSKAKAAELEEKLSKINDRWQHLFDVIGSRVKKLKETFAFIQQLDKNMSNLRTWLARIESELSKPVVYDVCDNQEIQKRLAEQQDLQRDIEQHSAGVESVFNICDVLLHDSDACANETECDSIQQTTRSLDRRWRNICAMSMERRMKIEETWRLWQKFLDDYSRFEDWLKSAERTAACPNSSEVLYTNAKEELKRFEAFQRQIHERLTQLELINKQYRRLARENRTDTASKLKQMVHEGNQRWDNLQKRVTAILRRLRYFTNQREEFEGTRESILVWLTEMDLQLTNVEHFSESDAEDKMRQLNGFQQEITLNTNKIDQLIVFGEQLIQKSEPLDAVLIEDELEELHRYCQEVFGRVSRFHRRLTSHTPGLDDEKEASENETDIEDPREIQADSWRKRRESEEPTSPQSLCHLVPPALGHERSGCETPVSVDSIPLEWDHTGDVGGSSSHEDDEEGPFYSALSGKSISEGHPWHVPDSPSHSKHHYKHMEGDRTEAPVPTDASTPFKSDYVKLLLRQGTDDSKEGLKEAQQEDEQLATLTGQQPGAFDRWELIQAQELHSKLRLKQTVQQLKSDIGSIAAWLGKTEAELEALKLAEPPSDIQEIALRVKRLQEILKAFDTYKALMVSVNVSHKEYLPSQSPEATELQNRLHQLSLSWDSVQGVLDSWRGDLRQSLMQCQDFHQLSQDLLLWLATAESRRQKAHVTSPEADRQVLLECQKDLMRLEKELVARQPQVSSLREISSSLLVKGQGEDYIEAEEKVHVIEKKLKQLQEQVAQDLMSLQRSLDPDASLTSFDEVDSGEQLPAAFAKSPRPRWTFLEEEEEEEETDSRMPHLDSPGSSQPRRSFLSRVIRAALPLQLLLLLLLLLACLLPASEDDYSCTQANNFARSFYPMLRYTNGPPPT 379792E42B95B7DB tm201_mouse Transmembrane protein 201 Net5 Samp1 Spindle-associated membrane protein 1 Tmem201 D4Ertd429e protein protein mouse Mus musculus Mouse MEGVSALLASCPTAGLAGGLGVTACAAAGVVLYRIARRVKPTHTMVNCWFCNHDTLVPYGNRNCWDCPHCEQYNGFQENGDYNKPIPAQYMEHLNHVVSSVPSPRDPAQPQQWVSSQVLLCRRCSHHQTTKIKQLAAFTPREEGRYDEEIEVYRHHLEQMYKLCRPCQAAVEYYIKHQNRQLRALLLSHQFRRREADQAHGQSFSSSAVKAPFQVILLRALAFLACAFLLFTTLYGPSEPFTPGAALPPALPPGGNSSAASDNTTSQAEGWQQLLGLLPEHATEKLHEAWAFGQSHQTSIVAVGLLTCLLAMLLAGRIRLRRIDAFSTCLWALLLGLHLAEHYLQAASPGWLDTLKFSTTSLCCLVGFTAAVATRKSTGPRRFRPRRYFSGDSASLFPSSPSLAVPYPSVTSSPASLFIPTPPGFLPLTKQQLFRSPRRVSPSSLPGRLSRALSLGTIPPLTRTDSGYLFSGSRPPSRVSPAGEVSLSDYFSLLSSSFPASPLPSPAPSVASSVASSSGSLRHRRPLISPARLNLKGQKLLLFSSPGEAPNTPSSSEEFSPPNGSLFIESPQLPQRNHTRDTKHTMEMRSMLARDSARSSHSIKKEDESSQSSTCVVDTTTKGCSEETTPWKARVSPSLVRGLLAVSLAVNALFTSAYLYQSLR mint FF1207C8C15B33C2 lmna_mouse Prelamin-A/C Lmna Lmn1 protein protein mouse Mus musculus Mouse METPSQRRATRSGAQASSTPLSPTRITRLQEKEDLQELNDRLAVYIDRVRSLETENAGLRLRITESEEVVSREVSGIKAAYEAELGDARKTLDSVAKERARLQLELSKVREEFKELKARNTKKEGDLLAAQARLKDLEALLNSKEAALSTALSEKRTLEGELHDLRGQVAKLEAALGEAKKQLQDEMLRRVDAENRLQTLKEELDFQKNIYSEELRETKRRHETRLVEIDNGKQREFESRLADALQELRAQHEDQVEQYKKELEKTYSAKLDNARQSAERNSNLVGAAHEELQQSRIRIDSLSAQLSQLQKQLAAKEAKLRDLEDSLARERDTSRRLLAEKEREMAEMRARMQQQLDEYQELLDIKLALDMEIHAYRKLLEGEEERLRLSPSPTSQRSRGRASSHSSQSQGGGSVTKKRKLESSESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIRRQNGDDPLMTYRFPPKFTLKAGQVVTIWASGAGATHSPPTDLVWKAQNTWGCGSSLRTALINSTGEEVAMRKLVRSLTMVEDNEDDDEDGEELLHHHRGSHCSGSGDPAEYNLRSRTVLCGTCGQPADKAAGGAGAQVGGSISSGSSASSVTVTRSFRSVGGSGGGSFGDNLVTRSYLLGNSSPRSQSSQNCSIM DIP protein P48678 original sequence version: 150 5434F574803FCB15 sun2_mouse SUN domain-containing protein 2 Sad1/unc-84 protein-like 2 Sun2 Unc84b Protein unc-84 homolog B protein protein mouse Mus musculus Mouse MSRRSQRLTRYSQDDNDGGSSSSGASSVAGSQGTVFKDSPLRTLKRKSSNMKHLSPAPQLGPSSDSHTSYYSESVVRESYIGSPRAVSLARSALLDDHLHSEPYWSGDLRGRRRRGTGGSESSKANGLTAESKASEDFFGSSSGYSSEDDLAGYTDSDQHSSGSRLRSAASRAGSFVWTLVTFPGRLFGLLYWWIGTTWYRLTTAASLLDVFVLTRSRHFSLNLKSFLWFLLLLLLLTGLTYGAWHFYPLGLQTLQPAVVSWWAAKESRKQPEVWESRDASQHFQAEQRVLSRVHSLERRLEALAADFSSNWQKEAIRLERLELRQGAAGHGGGSSLSHEDALSLLEGLVSRREATLKEDLRRDTVAHIQEELATLRAEHHQDSEDLFKKIVQASQESEARVQQLKTEWKSMTQEAFQESSVKELGRLEAQLASLRQELAALTLKQNSVADEVGLLPQKIQAARADVESQFPDWIRQFLLGDRGARSGLLQRDEMHAQLQELENKILTKMAEMQGKSAREAAASLGQILQKEGIVGVTEEQVHRIVKQALQRYSEDRIGMVDYALESGGASVISTRCSETYETKTALLSLFGIPLWYHSQSPRVILQPDVHPGNCWAFQGPQGFAVVRLSARIRPTAVTLEHVPKALSPNSTISSAPKDFAIFGFDEDLQQEGTLLGTFAYDQDGEPIQTFYFQASKMATYQVVELRILTNWGHPEYTCIYRFRVHGEPAH 67830D40C33E3DBA a2a8u2-3 Transmembrane protein 201 Spindle-associated membrane protein 1 Samp1b Tmem201 D4Ertd429e Net5 Samp1 protein protein mouse Mus musculus Mouse MEGVSALLASCPTAGLAGGLGVTACAAAGVVLYRIARRVKPTHTMVNCWFCNHDTLVPYGNRNCWDCPHCEQYNGFQENGDYNKPIPAQYMEHLNHVVSSVPSPRDPAQPQQWVSSQVLLCRRCSHHQTTKIKQLAAFTPREEGRYDEEIEVYRHHLEQMYKLCRPCQAAVEYYIKHQNRQLRALLLSHQFRRREADQAHGQSFSSSAVKAPFQVILLRALAFLACAFLLFTTLYGPSEPFTPGAALPPALPPGGNSSAASDNTTSQAEGWQQLLGLLPEHATEKLHEAWAFGQSHQTSIVAVGLLTCLLAMLLAGRIRLRRIDAFSTCLWALLLGLHLAEHYLQAASPGWLDTLKFSTTSLCCLVGFTAAVATRKSTGPRRFRPRRYFSGDSASLFPSSPSLAVPYPSVTSSPASLFIPTPPGFLPLTKQQLFRSPRRVSPSSLPGRLSRALSLGTIPPLTRTDSGYLFSGSRPPSRVSPAGEVSLSDYFSLLSSSFPASPLPSPAPSVASSVASSSGSLRHRRPLISPARLNLKGQKLLLFSSPGEAPNTPSSSEEFSPPNGSLFIESPQLPQRNHTRDTKHTMEMRSMLARDSARSSHSIKKEDESSQSSTCVVDTTTKGCSEETTPWKGL 61A5102FA5DD600D tmem201-sun2-1 3 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 3B tmem201-sun2-2 1 7 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 western blot western blot Immuno blot Immuno blot anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey association association 3C tmem201-lmna-1 3 8 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey physical association physical association 3E tmem201-sun2-4 2 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection c_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 3A F-actin is colocalized (staining with rhodamine-phalloidin). syne2-sun2-3 3 4 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain a fusion protein construct (GFP-mini-nesprin2G) built from the two indicated regions has been used region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association 3B syne2-tmem201-1 2 5 unspecified role unspecified role neutral component neutral component 4 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain a fusion protein construct (GFP-mini-nesprin2G) built from the two indicated regions has been used region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 2B F-actin is colocalized (staining with rhodamine-phalloidin).