IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Molecular and cellular biology 2012 imex curation Mikolcevic P., Sigl R., Rauch V., Hess MW., Pfaller K., Barisic M., Pelliniemi LJ., Boesl M., Geley S. stephan.geley@i-med.ac.at 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mikolcevic P., Sigl R., Rauch V., Hess MW., Pfaller K., Barisic M., Pelliniemi LJ., Boesl M., Geley S. stephan.geley@i-med.ac.at Accepted 2016-MAR-21 AT 11:03 GMT AT 11:03 GMT by MGT Molecular and cellular biology 2012 Only protein-protein interactions imex curation imex curation I'm not clear which you want me to add - BC041636 and BC028580 are not in UniProt and i have deliberately not added the promiscuous domain proteins and known false positive, for obvious reasons.; I've added a comment as the author appears to be regarding this as a negative expt in both orientations. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Molecular and cellular biology 2012 imex curation Mikolcevic P., Sigl R., Rauch V., Hess MW., Pfaller K., Barisic M., Pelliniemi LJ., Boesl M., Geley S. stephan.geley@i-med.ac.at 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-testis Mus musculus testis testis anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mikolcevic P., Sigl R., Rauch V., Hess MW., Pfaller K., Barisic M., Pelliniemi LJ., Boesl M., Geley S. stephan.geley@i-med.ac.at Accepted 2016-MAR-21 AT 11:03 GMT AT 11:03 GMT by MGT Molecular and cellular biology 2012 Only protein-protein interactions imex curation imex curation I'm not clear which you want me to add - BC041636 and BC028580 are not in UniProt and i have deliberately not added the promiscuous domain proteins and known false positive, for obvious reasons.; I've added a comment as the author appears to be regarding this as a negative expt in both orientations. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Molecular and cellular biology 2012 imex curation Mikolcevic P., Sigl R., Rauch V., Hess MW., Pfaller K., Barisic M., Pelliniemi LJ., Boesl M., Geley S. stephan.geley@i-med.ac.at 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Mikolcevic P., Sigl R., Rauch V., Hess MW., Pfaller K., Barisic M., Pelliniemi LJ., Boesl M., Geley S. stephan.geley@i-med.ac.at Accepted 2016-MAR-21 AT 11:03 GMT AT 11:03 GMT by MGT Human testis cDNA Molecular and cellular biology 2012 Only protein-protein interactions imex curation imex curation I'm not clear which you want me to add - BC041636 and BC028580 are not in UniProt and i have deliberately not added the promiscuous domain proteins and known false positive, for obvious reasons.; I've added a comment as the author appears to be regarding this as a negative expt in both orientations. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Molecular and cellular biology 2012 imex curation Mikolcevic P., Sigl R., Rauch V., Hess MW., Pfaller K., Barisic M., Pelliniemi LJ., Boesl M., Geley S. stephan.geley@i-med.ac.at 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mikolcevic P., Sigl R., Rauch V., Hess MW., Pfaller K., Barisic M., Pelliniemi LJ., Boesl M., Geley S. stephan.geley@i-med.ac.at Accepted 2016-MAR-21 AT 11:03 GMT AT 11:03 GMT by MGT Molecular and cellular biology 2012 Only protein-protein interactions imex curation imex curation I'm not clear which you want me to add - BC041636 and BC028580 are not in UniProt and i have deliberately not added the promiscuous domain proteins and known false positive, for obvious reasons.; I've added a comment as the author appears to be regarding this as a negative expt in both orientations. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Cyclin-dependent kinase 16/PCTAIRE kinase 1 is activated by cyclin Y and is essential for spermatogenesis. Molecular and cellular biology 2012 imex curation Mikolcevic P., Sigl R., Rauch V., Hess MW., Pfaller K., Barisic M., Pelliniemi LJ., Boesl M., Geley S. stephan.geley@i-med.ac.at 11-Apr-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293a Homo sapiens hek293a hek293a anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Mikolcevic P., Sigl R., Rauch V., Hess MW., Pfaller K., Barisic M., Pelliniemi LJ., Boesl M., Geley S. stephan.geley@i-med.ac.at Accepted 2016-MAR-21 AT 11:03 GMT AT 11:03 GMT by MGT Molecular and cellular biology 2012 Only protein-protein interactions imex curation imex curation I'm not clear which you want me to add - BC041636 and BC028580 are not in UniProt and i have deliberately not added the promiscuous domain proteins and known false positive, for obvious reasons.; I've added a comment as the author appears to be regarding this as a negative expt in both orientations. ccyl1_human Cyclin-Y-like protein 1 CCNYL1 protein protein human Homo sapiens Human MGNTLTCCVSPNASPKLGRRAGSAELYCASDIYEAVSGDAVAVAPAVVEPAELDFGEGEGHHLQHISDREMPEDLALESNPSDHPRASTIFLSKSQTDVREKRKSNHLNHVSPGQLTKKYSSCSTIFLDDSTVSQPNLRTTVKCVTLAIYYHIKNRDANRSLDIFDERSHPLTREKVPEEYFKHDPEHKFIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPTNWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNEMERHFLELLQFNINVPASVYAKYYFDLRSLADDNNLNFLFAPLSKERAQNLEAISRLCEDKDLCRAAMRRSFSADNFIGIQRSKAILS 76381F33FE5C890C mi4gd_human MIF4G domain-containing protein SLIP1 SLBP-interacting protein 1 MIF4GD protein protein human Homo sapiens Human MGEPSREEYKIQSFDAETQQLLKTALKDPGAVDLEKVANVIVDHSLQDCVFSKEAGRMCYAIIQAESKQAGQSVFRRGLLNRLQQEYQAREQLRARSLQGWVCYVTFICNIFDYLRVNNMPMMALVNPVYDCLFRLAQPDSLSKEEEVDCLVLQLHRVGEQLEKMNGQRMDELFVLIRDGFLLPTGLSSLAQLLLLEIIEFRAAGWKTTPAAHKYYYSEVSD 07E5B651D638AC9D spdya_human Speedy protein A SPDYA SPDY1 SPY1 Speedy-1 Rapid inducer of G2/M progression in oocytes A protein protein human Homo sapiens Human MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQDMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEETKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYTGSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE mint F511EECB26245EEC ptps_human 6-pyruvoyl tetrahydrobiopterin synthase PTS protein protein human Homo sapiens Human MSTEGGGRRCQAQVSRRISFSASHRLYSKFLSDEENLKLFGKCNNPNGHGHNYKVVVTVHGEIDPATGMVMNLADLKKYMEEAIMQPLDHKNLDMDVPYFADVVSTTENVAVYIWDNLQKVLPVGVLYKVKVYETDNNIVVYKGE A1CD0DC2F83187E0 cdk16_mouse Cyclin-dependent kinase 16 CRK5 Cell division protein kinase 16 PCTAIRE-motif protein kinase 1 Serine/threonine-protein kinase PCTAIRE-1 Cdk16 Crk5 Pctaire1 Pctk1 protein protein mouse Mus musculus Mouse MDRMKKIKRQLSMTLRGGRGIDKTNGVPEQIGLDESGGGGGSDLGEAPTRIAPGELRSVRGPLSSAPEIVHEDMKMGSDGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPEGYLEKLTLNSPIFDKPLSRRLRRVSLSEIGFGKLETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNVINMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFRTYNYPKYRAEALLSHAPRLDSDGADLLTKLLQFEGRNRISAEDARKHPFFLSLGERIHKLPDTTSIFALKEVQLQKEANIRSTSMPDSGRPAFRVVDTEF 0746036524B1FB5F cdk16_human Cyclin-dependent kinase 16 PCTAIRE-motif protein kinase 1 CDK16 PCTK1 Serine/threonine-protein kinase PCTAIRE-1 Cell division protein kinase 16 PCTAIRE1 protein protein human Homo sapiens Human MDRMKKIKRQLSMTLRGGRGIDKTNGAPEQIGLDESGGGGGSDPGEAPTRAAPGELRSARGPLSSAPEIVHEDLKMGSDGESDQASATSSDEVQSPVRVRMRNHPPRKISTEDINKRLSLPADIRLPEGYLEKLTLNSPIFDKPLSRRLRRVSLSEIGFGKLETYIKLDKLGEGTYATVYKGKSKLTDNLVALKEIRLEHEEGAPCTAIREVSLLKDLKHANIVTLHDIIHTEKSLTLVFEYLDKDLKQYLDDCGNIINMHNVKLFLFQLLRGLAYCHRQKVLHRDLKPQNLLINERGELKLADFGLARAKSIPTKTYSNEVVTLWYRPPDILLGSTDYSTQIDMWGVGCIFYEMATGRPLFPGSTVEEQLHFIFRILGTPTEETWPGILSNEEFKTYNYPKYRAEALLSHAPRLDSDGADLLTKLLQFEGRNRISAEDAMKHPFFLSLGERIHKLPDTTSIFALKEIQLQKEASLRSSSMPDSGRPAFRVVDTEF 9A5DDD34A5E5CBBB ccni_human Cyclin-I CCNI protein protein human Homo sapiens Human MKFPGPLENQRLSFLLEKAITREAQMWKVNVRKMPSNQNVSPSQRDEVIQWLAKLKYQFNLYPETFALASSLLDRFLATVKAHPKYLSCIAISCFFLAAKTVEEDERIPVLKVLARDSFCGCSSSEILRMERIILDKLNWDLHTATPLDFLHIFHAIAVSTRPQLLFSLPKLSPSQHLAVLTKQLLHCMACNQLLQFRGSMLALAMVSLEMEKLIPDWLSLTIELLQKAQMDSSQLIHCRELVAHHLSTLQSSLPLNSVYVYRPLKHTLVTCDKGVFRLHPSSVPGPDFSKDNSKPEVPVRGTAAFYHHLPAASGCKQTSTKRKVEEMEVDDFYDGIKRLYNEDNVSENVGSVCGTDLSRQEGHASPCPPLQPVSVM 2DE84EFA74698F6C ccny_human Cyclin-Y Cyclin fold protein 1 Cyclin box protein 1 CCNY C10orf9 CBCP1 CFP1 cyclin-X protein protein human Homo sapiens Human MGNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENIDDLNMEFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQIARKYSSCSTIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRERAHKLEAISRLCEDKYKDLRRSARKRSASADNLTLPRWSPAIIS 949FDE1EE45C2C6E cf20d_human Protein CFAP20DC CFAP20DC C3orf67 Uncharacterized protein C3orf67 protein protein human Homo sapiens Human MIKRKIWCNLCIDLVAFTSEIFKGAVFQSLDGIVVSANCKLRKIFTLKSKPQDTADKDAVYGVPFSTDEPTDIIPRSCQLMTDVPHVTQLLNMTKLRQTEIKFGGHPLRSAESDQFINRGTSITRNSKNQDVCHIAFGSKVLGPPPLSGRRNNMKISSETVRSVGSKNNRSCQPSTVEKCVNGTEMSALLIPESEEQGNKENIHQIKQTVPIHAANLHIMHPHPPQEPSADKNNNRRRLRLKSTSRERTETPSGSSSGNNRIEDKASTILTTVSQQGAELLNSGTLGPQSPDQSDEWIFPENADHISYLASSRQSLLLGDDSCNPSHLWLEASKESEHDQQAEESQSVPKDIFTFSSRPRSAPHGKTQTMSPEELSFILDLKEDNSVTSRDTQSEDDFYGGDSSEEGNHSIQGSRGPTTGPSELTQLTLESLLGKAAKRTSKEYLRSAYTEAGATESQDSSMEQIDRNNFEMSLLPTTCLSPTGRRCGSCQKTPEPVIKAKDLSAQQVPASLNKTSLKEISGERLSSIPEASEYDWRNYQPSQMSESELQMLASLRWQQNEELEDAGTSHGLSASQVDNCNVSISTSSDDTTTWNSCLPPPVNQGRHYQKEMNPPSPSNPRDWLNMLSPPIVPPSQQPAEQRPDSCESLSVQGEEDLSVEEDEEVLTLLYDPCLNCYFDPQTGKYYELV 0CA8DEB6C9730DF8 tmm99_human Uncharacterized protein KRT10-AS1 KRT10-AS1 TMEM99 Transmembrane protein 99 protein protein human Homo sapiens Human MVGILPLCCSGCVPSLCCSSYVPSVAPTAAHSVRVPHSAGHCGQRVLACSLPQVFLKPWIFVEHFSSWLSLELFSFLRYLGTLLCACGHRLREGLLLPCLLGVGSWLLFNNWTGGSWFSLHLQQVSLSQGSHVAAFLPEAIGPGVPVPVSGESTSAQQSHAGWQLSAEADACPSVLYSEVLEWNKNINTYTSFHDFCLILGIFLFCFVLAVIGLPYIKPGLSLSVALLWQSLILLSSLVQQDSQVHTWGCLFSTFTST 857594EF02E0FA38 uap1_human UDP-N-acetylhexosamine pyrophosphorylase UAP1 Antigen X SPAG2 Sperm-associated antigen 2 protein protein human Homo sapiens Human MNINDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSHQKNVDARMEPVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQAERILKLQQVAEKYYGNKCIIPWYIMTSGRTMESTKEFFTKHKYFGLKKENVIFFQQGMLPAMSFDGKIILEEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVVEKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQKRSSDGRLLFNAGNIANHFFTVPFLRDVVNVYEPQLQHHVAQKKIPYVDTQGQLIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKDNPTTARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRSATNGKSETITADVNHNLKDANDVPIQCEISPLISYAGEGLESYVADKEFHAPLIIDENGVHELVKNGI 8A69B36D852B9472 q8nd76-1 Cyclin-Y Cyclin box protein 1 Cyclin fold protein 1 cyclin-X b CCNY C10orf9 CBCP1 CFP1 protein protein human Homo sapiens Human MGNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENIDDLNMEFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQIARKYSSCSTIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRERAHKLEAISRLCEDKYKDLRRSARKRSASADNLTLPRWSPAIIS 949FDE1EE45C2C6E ccnk_human Cyclin-K CCNK CPR4 protein protein human Homo sapiens Human MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQPTPQVPQVQQSQPSQSSEPSQPQQKDPQQPAQQQQPAQQPKKPSPQPSSPRQVKRAVVVSPKEENKAAEPPPPKIPKIETTHPPLPPAHPPPDRKPPLAAALGEAEPPGPVDATDLPKVQIPPPAHPAPVHQPPPLPHRPPPPPPSSYMTGMSTTSSYMSGEGYQSLQSMMKTEGPSYGALPPAYGPPAHLPYHPHVYPPNPPPPPVPPPPASFPPPAIPPPTPGYPPPPPTYNPNFPPPPPRLPPTHAVPPHPPPGLGLPPASYPPPAVPPGGQPPVPPPIPPPGMPPVGGLGRAAWMR DIP protein O75909 original sequence version: 158 8A945E90359AD9F8 ccny_mouse Cyclin-Y Cyclin fold protein 1 Ccny Cfp1 protein protein mouse Mus musculus Mouse MGNTTSCCVSSSPKLRRNAHSRLESYRPDTDLSREDTGCNLQHISDRENIDDLNMEFNPSDHPRASTIFLSKSQTDVREKRKSLFINHHPPGQTSRKYSSCSTIFLDDSTVSQPNLKYTIKCVALAIYYHIKNRDPDGRMLLDIFDENLHPLSKSEVPPDYDKHNPEQKQIYRFVRTLFSAAQLTAECAIVTLVYLERLLTYAEIDICPANWKRIVLGAILLASKVWDDQAVWNVDYCQILKDITVEDMNELERQFLELLQFNINVPSSVYAKYYFDLRSLAEANNLSFPLEPLSRERAHKLEAISRLCEDKYKDLRKPMRKRSASADNLILPRWSPAIIS 6F16CE5B14559FCB fbx8_human F-box only protein 8 FBXO8 FBS FBX8 DC10 UNQ1877/PRO4320 F-box/SEC7 protein FBS protein protein human Homo sapiens Human MGQGLWRVVRNQQLQQEGYSEQGYLTREQSRRMAASNISNTNHRKQVQGGIDIYHLLKARKSKEQEGFINLEMLPPELSFTILSYLNATDLCLASCVWQDLANDELLWQGLCKSTWGHCSIYNKNPPLGFSFRKLYMQLDEGSLTFNANPDEGVNYFMSKGILDDSPKEIAKFIFCTRTLNWKKLRIYLDERRDVLDDLVTLHNFRNQFLPNALREFFRHIHAPEERGEYLETLITKFSHRFCACNPDLMRELGLSPDAVYVLCYSLILLSIDLTSPHVKNKMSKREFIRNTRRAAQNISEDFVGHLYDNIYLIGHVAA 125D6D6B57CA7C79 cdk16-ccni-2 3 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-ccnk-1 3 18 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-ccny-1 3 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-ccnyl1-3 3 6 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-spdya-1 3 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-fbxo8-1 3 20 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-mif4gd-1 3 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-c3orf67-1 3 14 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-pts-1 3 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-tmem99-1 3 15 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-uap1-1 3 16 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Table 1 cdk16-ccny-2 5 17 unspecified role unspecified role prey prey n_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8ND76-1:p.Leu222_Ser224delinsAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain LAS AAA Wed May 02 20:29:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_ala_ser222-224ala_ala_ala' Tue Jun 30 16:20:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu222_Ser224delinsAlaAlaAla' 11 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q00536:p.Ser119Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:50:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser119ala' Tue Jun 30 16:54:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser119Ala' undefined mutation mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:02:36 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'ser153ala' undefined mutation mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:02:34 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'ser153asp' undefined mutation mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:02:34 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'gly158asp' undefined mutation mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:02:32 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'ile209thr' undefined mutation mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:02:33 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'lys216glu' ser-153 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain disrupting-ptm ptm disrupting an interaction disrupting-ptm physical association physical association Fig. 1B, 1C, 2C, 2D, 2E, 3B, 6D, 7A Staurosporine (CHEBI:15738) Forskolin (CHEBI:42471) ccny-cdk16-1 5 17 unspecified role unspecified role bait bait n_terminal_region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain n-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8ND76-1:p.Leu222_Ser224delinsAlaAlaAla mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain LAS AAA Wed May 02 19:37:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu_ala_ser222-224ala_ala_ala' Tue Jun 30 18:41:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu222_Ser224delinsAlaAlaAla' 11 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 3B ccny-cdk16-2 5 17 unspecified role unspecified role bait bait n-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain Q00536:p.Ser119Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:54:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser119ala' Tue Jun 30 18:44:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser119Ala' undefined mutation mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:47:16 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'ser153ala' undefined mutation mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:47:08 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'ser153asp' undefined mutation mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:47:09 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'gly158asp' undefined mutation mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:47:07 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'ile209thr' undefined mutation mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:47:10 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'lys216glu' physical association physical association Fig. 1B, 1C, 2C, 2D, 2E. ccny-cdk16-4 5 17 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 11 unspecified role unspecified role prey prey n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 6E ccny-cdk16-5 4 11 putative self putative self putative self putative self region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q00536:p.Ser153Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:13:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser153ala' Tue Jun 30 18:23:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser153Ala' ecoli Escherichia coli (strain K12) 17 enzyme regulator enzyme regulator neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 7B ccny-cdk16-6 4 17 enzyme regulator enzyme regulator neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q00536:p.Ser153Ala mutation mutation certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:09:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser153ala' Tue Jun 30 19:48:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser153Ala' ecoli Escherichia coli (strain K12) 17 enzyme target enzyme target substrate substrate neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation Fig. 7Bi cdk16-ccny-3 1 10 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey physical association physical association Fig. 8C cdk16-ccny-4 2 10 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey physical association physical association Fig. 8C cdk16-ccny-5 5 17 unspecified role unspecified role prey prey c-terminal yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 11 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Fig. 1B